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The map label for this gene is gmd [H]

Identifier: 159900259

GI number: 159900259

Start: 4704929

End: 4705891

Strand: Reverse

Name: gmd [H]

Synonym: Haur_3742

Alternate gene names: 159900259

Gene position: 4705891-4704929 (Counterclockwise)

Preceding gene: 159900260

Following gene: 159900257

Centisome position: 74.15

GC content: 53.27

Gene sequence:

>963_bases
ATGCGTGTTTTGATTACTGGCATTACTGGGCCAGTTGGTAGTTTTCTCGCCGATTATCTGTTAACCTTGCCTGATCTCGA
AATTCATGCGCTCAAGCGTTGGCGCTCCGATCCACGGCCAATTGAGCATTTGCAAGGCAAAGTAACCTTCCACGAAGGCG
ATATTGAAGATGCCTTTGCGATGGCTACCTTGCTGCGGCGCGTGCAGCCAGAGCGCATTTTTCACCTTGCGGCCCAAAGC
TACCCTAGCGCCTCATGGGATGCTCCAGTTACCACCTTACGTGCCAACACCGAAGGAACCTTAAATATTTTAGAGGCGGT
ACGGGTTACCGTACCCCAAGCCCGTTTGCATATTGCTTGCACCAGCGCCCAGTATGGCATCGTTAATCCGGCTGATGTGC
CGATTAAGGAGAGCCACCCCTTGCGCCCAGGCAGCCCATATGGCGTGAGCAAGGCCGCCGCCGAAATGTTGGGTTTGCAA
TATCATCAATCATATGGCTTGCATGTGGTGGTTACACGCTCGTTTAATCATGTTGGGCCACGCCAAGGCGATCGCTGCTC
GATTCAAACATTTTGCCAGCAGATGGCGGCAATTGAAGCAGGCCAGAGCGAGCCAGTGATCCATGTTGGCAATTTGGAGC
CACAGCGCGATTTCAGCCATTTCAGCGATGTGGCCCGGGCTTTGTGGCTGCTGCTCGAAAAAGCGCCTCCTGGCGAGGTT
TACAATCTGTGTTCAGGCGTGGCTACCCGCATTGGCGATATTGTGGAGTTGGTGCGACGATTCGGGCGTGTGCCTATCGA
AGTTAGGCTCGACCCAAGCCGTTTACGCCCAGTCGATGAGCCAATTTTGCAAGGCGATAACAGCAAATTGCGCCAAGCCA
CTGGCTGGGAGCCACAAATTGGTATGGAGCAAATTGTGCAAGAAGTGCTGGATTTTTGGCGCGAACGCTACGGTATTTTG
TAG

Upstream 100 bases:

>100_bases
GCACAACCTTGGTTTTGCCAGCAAGTTTGGGCGCATACACCTACGAAGTTGCCAACTCGGTGACCCTGCTACGCTGCTAT
GTACCTACTGAGGAACTCTA

Downstream 100 bases:

>100_bases
CGTTCGTGCTGGTGCTCAGCCTGCCACACATTGATGCTATGTAGGTTGTTGATTGAGCACCATCTTGGCGTAAACTCCAT
TAGTGCCCACTAACTCGCTA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRVLITGITGPVGSFLADYLLTLPDLEIHALKRWRSDPRPIEHLQGKVTFHEGDIEDAFAMATLLRRVQPERIFHLAAQS
YPSASWDAPVTTLRANTEGTLNILEAVRVTVPQARLHIACTSAQYGIVNPADVPIKESHPLRPGSPYGVSKAAAEMLGLQ
YHQSYGLHVVVTRSFNHVGPRQGDRCSIQTFCQQMAAIEAGQSEPVIHVGNLEPQRDFSHFSDVARALWLLLEKAPPGEV
YNLCSGVATRIGDIVELVRRFGRVPIEVRLDPSRLRPVDEPILQGDNSKLRQATGWEPQIGMEQIVQEVLDFWRERYGIL

Sequences:

>Translated_320_residues
MRVLITGITGPVGSFLADYLLTLPDLEIHALKRWRSDPRPIEHLQGKVTFHEGDIEDAFAMATLLRRVQPERIFHLAAQS
YPSASWDAPVTTLRANTEGTLNILEAVRVTVPQARLHIACTSAQYGIVNPADVPIKESHPLRPGSPYGVSKAAAEMLGLQ
YHQSYGLHVVVTRSFNHVGPRQGDRCSIQTFCQQMAAIEAGQSEPVIHVGNLEPQRDFSHFSDVARALWLLLEKAPPGEV
YNLCSGVATRIGDIVELVRRFGRVPIEVRLDPSRLRPVDEPILQGDNSKLRQATGWEPQIGMEQIVQEVLDFWRERYGIL
>Mature_320_residues
MRVLITGITGPVGSFLADYLLTLPDLEIHALKRWRSDPRPIEHLQGKVTFHEGDIEDAFAMATLLRRVQPERIFHLAAQS
YPSASWDAPVTTLRANTEGTLNILEAVRVTVPQARLHIACTSAQYGIVNPADVPIKESHPLRPGSPYGVSKAAAEMLGLQ
YHQSYGLHVVVTRSFNHVGPRQGDRCSIQTFCQQMAAIEAGQSEPVIHVGNLEPQRDFSHFSDVARALWLLLEKAPPGEV
YNLCSGVATRIGDIVELVRRFGRVPIEVRLDPSRLRPVDEPILQGDNSKLRQATGWEPQIGMEQIVQEVLDFWRERYGIL

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=337, Percent_Identity=30.8605341246291, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=25.4601226993865, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=28.3987915407855, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=29.971181556196, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=27.0348837209302, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=26.8011527377522, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786974, Length=343, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=337, Percent_Identity=30.2670623145401, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17507723, Length=337, Percent_Identity=30.8605341246291, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI133901788, Length=337, Percent_Identity=30.2670623145401, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17539422, Length=337, Percent_Identity=30.2670623145401, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17539424, Length=337, Percent_Identity=30.2670623145401, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI133901790, Length=337, Percent_Identity=30.2670623145401, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=28.3132530120482, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=25.0746268656716, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=30.4733727810651, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=27.6595744680851, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 35715; Mature: 35715

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLITGITGPVGSFLADYLLTLPDLEIHALKRWRSDPRPIEHLQGKVTFHEGDIEDAFA
CEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCEEEEECCCHHHHHH
MATLLRRVQPERIFHLAAQSYPSASWDAPVTTLRANTEGTLNILEAVRVTVPQARLHIAC
HHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
TSAQYGIVNPADVPIKESHPLRPGSPYGVSKAAAEMLGLQYHQSYGLHVVVTRSFNHVGP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RQGDRCSIQTFCQQMAAIEAGQSEPVIHVGNLEPQRDFSHFSDVARALWLLLEKAPPGEV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
YNLCSGVATRIGDIVELVRRFGRVPIEVRLDPSRLRPVDEPILQGDNSKLRQATGWEPQI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHCCCCHHHHHCCCCCCCC
GMEQIVQEVLDFWRERYGIL
CHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVLITGITGPVGSFLADYLLTLPDLEIHALKRWRSDPRPIEHLQGKVTFHEGDIEDAFA
CEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHCCEEEEECCCHHHHHH
MATLLRRVQPERIFHLAAQSYPSASWDAPVTTLRANTEGTLNILEAVRVTVPQARLHIAC
HHHHHHHHCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEE
TSAQYGIVNPADVPIKESHPLRPGSPYGVSKAAAEMLGLQYHQSYGLHVVVTRSFNHVGP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RQGDRCSIQTFCQQMAAIEAGQSEPVIHVGNLEPQRDFSHFSDVARALWLLLEKAPPGEV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
YNLCSGVATRIGDIVELVRRFGRVPIEVRLDPSRLRPVDEPILQGDNSKLRQATGWEPQI
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHCCCCHHHHHCCCCCCCC
GMEQIVQEVLDFWRERYGIL
CHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]