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The map label for this gene is rlmI [H]

Identifier: 159900218

GI number: 159900218

Start: 4654885

End: 4656069

Strand: Direct

Name: rlmI [H]

Synonym: Haur_3701

Alternate gene names: 159900218

Gene position: 4654885-4656069 (Clockwise)

Preceding gene: 159900217

Following gene: 159900219

Centisome position: 73.34

GC content: 54.18

Gene sequence:

>1185_bases
ATGTTGCGGTTTATTCTCAAACCTGATCGTGATAAATCAATTCGCCAACGCCATCCATGGGTTTTCAGCGGGGCGGTTGC
CCGTCGTTCTGGCCCAGTGCATCCTGGCGAAACCGTCGATATTATTAGCTCCGAGGGGGTGTGGTTGGCGCGGGGCACTG
CCAGCACCCACTCGCAAATGGTCGCCCGCCTGTGGACGTGGCAACGCGACGAGGAGCTAGACCCCAGTTTTATTCGCCAA
CGGATCGAACGAGCAATCAAAGGGCGCGAACAGCTCCATAATGATCCCCAAACCAATGCTTATCGCGTAATTTTTAGCGA
AAGCGATGGCTTGCCAGGCTTAATCGTCGATCGTTTTGCTGATTGGCTAGTGGTGCAATTGCTGACTCAAGGTGCAGTTG
CTCACACGGCGATTATCGTCGCAGCCTTGGCCGAATTGATGCCAGTCCGCGGGATTTACGAGCGCTCTGATGTTGAAATT
CGTGCTCGTGAAGGCTTGGGTGAGGCTGAAGGCCTGCTTTGGGGCGAAGCACCACCCGATCAATTAACTATTCGCGAAAA
TGGCTACGAGTTTGTGCTCGATTTGGCGGGCGGCCAAAAAACTGGCTGGTACGTTGATCAACGCATCAATCGCCAGCGCG
TGGCCCAATATGCCAAGGATGCCGAAGTGCTGGGGGTTTTCTCCTATACTGGCGGGTTTGAGGTGCTAGCGGCGGGCGCT
GGCGCTCAATCAATCACAGCGGTTGATAGTTCGGCGGCGGCCTTGCGTGGCTTACATACCAACCTCAGCCAAAATGGGCT
TAACACTCCAGTTACGGCGGTCGAAGGCGATGCCTTCAAGATTTTGCGCCAACTACGCGAAGAAGGCAAACAGTACGATC
TGATTGTGCTTGACCCACCCAAGTTTGCCCACTCGCAGAGCCAAATTGATCGAGCAACGCGTGGCTACAAAGATATCAAT
ATGCAAGCCTTCCACTTGCTACGACCAGGCGGCGTGTTGGCAACCTTCTCATGTTCAGGGCTGATTAGCGCCGATCTCTT
TCAAAAAGTGGTGTTTGGCGCAGCCTTGGATGCTGGTCGCGATGCCCAAATTATCGACACACTCACCCAAGGTGGTGATC
ACCCTGTCTTGTTGACCTTCCCAGAGGCGGCTTATCTCAAGGGATTAATCTGCCGCGTCTGGTAG

Upstream 100 bases:

>100_bases
ACTGCGTTATACTCTCACCAGAGGATTTCAGGATCGCGAAATTTCTCCCGTATCACTCAACCTGCTAGGCTCTGACCTAG
CCTTAAAGCGAGCAAAAGGT

Downstream 100 bases:

>100_bases
CCGTTGAGGATTTGCAATGGCGTATCGTTTGCTCAATGAAGGCTCGATCACAACGCCTACTGGCTTTCGCGTAACAGGTA
TTCACTGCGGCTTGAAAACC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLRFILKPDRDKSIRQRHPWVFSGAVARRSGPVHPGETVDIISSEGVWLARGTASTHSQMVARLWTWQRDEELDPSFIRQ
RIERAIKGREQLHNDPQTNAYRVIFSESDGLPGLIVDRFADWLVVQLLTQGAVAHTAIIVAALAELMPVRGIYERSDVEI
RAREGLGEAEGLLWGEAPPDQLTIRENGYEFVLDLAGGQKTGWYVDQRINRQRVAQYAKDAEVLGVFSYTGGFEVLAAGA
GAQSITAVDSSAAALRGLHTNLSQNGLNTPVTAVEGDAFKILRQLREEGKQYDLIVLDPPKFAHSQSQIDRATRGYKDIN
MQAFHLLRPGGVLATFSCSGLISADLFQKVVFGAALDAGRDAQIIDTLTQGGDHPVLLTFPEAAYLKGLICRVW

Sequences:

>Translated_394_residues
MLRFILKPDRDKSIRQRHPWVFSGAVARRSGPVHPGETVDIISSEGVWLARGTASTHSQMVARLWTWQRDEELDPSFIRQ
RIERAIKGREQLHNDPQTNAYRVIFSESDGLPGLIVDRFADWLVVQLLTQGAVAHTAIIVAALAELMPVRGIYERSDVEI
RAREGLGEAEGLLWGEAPPDQLTIRENGYEFVLDLAGGQKTGWYVDQRINRQRVAQYAKDAEVLGVFSYTGGFEVLAAGA
GAQSITAVDSSAAALRGLHTNLSQNGLNTPVTAVEGDAFKILRQLREEGKQYDLIVLDPPKFAHSQSQIDRATRGYKDIN
MQAFHLLRPGGVLATFSCSGLISADLFQKVVFGAALDAGRDAQIIDTLTQGGDHPVLLTFPEAAYLKGLICRVW
>Mature_394_residues
MLRFILKPDRDKSIRQRHPWVFSGAVARRSGPVHPGETVDIISSEGVWLARGTASTHSQMVARLWTWQRDEELDPSFIRQ
RIERAIKGREQLHNDPQTNAYRVIFSESDGLPGLIVDRFADWLVVQLLTQGAVAHTAIIVAALAELMPVRGIYERSDVEI
RAREGLGEAEGLLWGEAPPDQLTIRENGYEFVLDLAGGQKTGWYVDQRINRQRVAQYAKDAEVLGVFSYTGGFEVLAAGA
GAQSITAVDSSAAALRGLHTNLSQNGLNTPVTAVEGDAFKILRQLREEGKQYDLIVLDPPKFAHSQSQIDRATRGYKDIN
MQAFHLLRPGGVLATFSCSGLISADLFQKVVFGAALDAGRDAQIIDTLTQGGDHPVLLTFPEAAYLKGLICRVW

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=47.5826972010178, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787181, Length=264, Percent_Identity=31.0606060606061, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43439; Mature: 43439

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFILKPDRDKSIRQRHPWVFSGAVARRSGPVHPGETVDIISSEGVWLARGTASTHSQM
CEEEEECCCCCCHHHHHCCEEEECHHHHCCCCCCCCCEEEEEECCCEEEEECCCHHHHHH
VARLWTWQRDEELDPSFIRQRIERAIKGREQLHNDPQTNAYRVIFSESDGLPGLIVDRFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHH
DWLVVQLLTQGAVAHTAIIVAALAELMPVRGIYERSDVEIRAREGLGEAEGLLWGEAPPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCC
QLTIRENGYEFVLDLAGGQKTGWYVDQRINRQRVAQYAKDAEVLGVFSYTGGFEVLAAGA
CEEEECCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHCCCCEEEEEEECCCEEEEEECC
GAQSITAVDSSAAALRGLHTNLSQNGLNTPVTAVEGDAFKILRQLREEGKQYDLIVLDPP
CCCEEEECCCHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEECCC
KFAHSQSQIDRATRGYKDINMQAFHLLRPGGVLATFSCSGLISADLFQKVVFGAALDAGR
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCC
DAQIIDTLTQGGDHPVLLTFPEAAYLKGLICRVW
CHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MLRFILKPDRDKSIRQRHPWVFSGAVARRSGPVHPGETVDIISSEGVWLARGTASTHSQM
CEEEEECCCCCCHHHHHCCEEEECHHHHCCCCCCCCCEEEEEECCCEEEEECCCHHHHHH
VARLWTWQRDEELDPSFIRQRIERAIKGREQLHNDPQTNAYRVIFSESDGLPGLIVDRFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHH
DWLVVQLLTQGAVAHTAIIVAALAELMPVRGIYERSDVEIRAREGLGEAEGLLWGEAPPD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCC
QLTIRENGYEFVLDLAGGQKTGWYVDQRINRQRVAQYAKDAEVLGVFSYTGGFEVLAAGA
CEEEECCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHCCCCEEEEEEECCCEEEEEECC
GAQSITAVDSSAAALRGLHTNLSQNGLNTPVTAVEGDAFKILRQLREEGKQYDLIVLDPP
CCCEEEECCCHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEECCC
KFAHSQSQIDRATRGYKDINMQAFHLLRPGGVLATFSCSGLISADLFQKVVFGAALDAGR
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCC
DAQIIDTLTQGGDHPVLLTFPEAAYLKGLICRVW
CHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA