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The map label for this gene is ntaB [H]

Identifier: 159900208

GI number: 159900208

Start: 4643757

End: 4644233

Strand: Reverse

Name: ntaB [H]

Synonym: Haur_3691

Alternate gene names: 159900208

Gene position: 4644233-4643757 (Counterclockwise)

Preceding gene: 159900212

Following gene: 159900207

Centisome position: 73.18

GC content: 50.94

Gene sequence:

>477_bases
ATGACGCTTGACCCTGCTTTGTTTCGGCAAGCAATGAGCCGTTTTGCCAGCGGTGTAACTGTAGTGACAACGATTGTGGA
TGCGAAGCCCTATGGTTTAACGGTTAGCGCCTTTGCCTCGCTCTCTTTAGATCCAGCTTTGGCGATTATCGCGATTGATA
AACGCTCGCCAATGCACGATCTGTTGCTCCAAGCTCAGGCTTTTGCGATCAATATCTTGAGCAACGAGCAAGAATCGTTG
TCGCGCCACTTTGCTGGCCCGCAAAAACACGATTGGTCGGAAATTGCGGCGGCGGCTGGCAATCGCAGTATTCCATTGCT
CGACGGAGCTTTGGCTAACATGGAATGCGGGCTGTTTCAGGCAGTTGATGGCGGCGATCATACGATTTTTATTGGTCAAG
TTGAGCATGTAACCCTGCGCGATGGTCTACCCTTGCTCTATTATCGTGGCGCTTACCACGATCTTGTTCAGCGTTGA

Upstream 100 bases:

>100_bases
CTTCGTGGATCAAACTGGCCCCTGATCCCTAGCCCCTAGCCTTGCATTGACATTTGAACCTCGTTACAATAGGCGTAGCA
ATCTGTATGAGGAATATTCG

Downstream 100 bases:

>100_bases
AACGAGCACGCAGCATGTGGTTGACCTTAATCTTATTTGTGGTTGGGCTGGGGCTGGCATTTGGCTTAGGAGTGATGGTT
GGCCGTCAACGCGGCTTCGC

Product: flavin reductase domain-containing protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTLDPALFRQAMSRFASGVTVVTTIVDAKPYGLTVSAFASLSLDPALAIIAIDKRSPMHDLLLQAQAFAINILSNEQESL
SRHFAGPQKHDWSEIAAAAGNRSIPLLDGALANMECGLFQAVDGGDHTIFIGQVEHVTLRDGLPLLYYRGAYHDLVQR

Sequences:

>Translated_158_residues
MTLDPALFRQAMSRFASGVTVVTTIVDAKPYGLTVSAFASLSLDPALAIIAIDKRSPMHDLLLQAQAFAINILSNEQESL
SRHFAGPQKHDWSEIAAAAGNRSIPLLDGALANMECGLFQAVDGGDHTIFIGQVEHVTLRDGLPLLYYRGAYHDLVQR
>Mature_157_residues
TLDPALFRQAMSRFASGVTVVTTIVDAKPYGLTVSAFASLSLDPALAIIAIDKRSPMHDLLLQAQAFAINILSNEQESLS
RHFAGPQKHDWSEIAAAAGNRSIPLLDGALANMECGLFQAVDGGDHTIFIGQVEHVTLRDGLPLLYYRGAYHDLVQR

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=159, Percent_Identity=28.3018867924528, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17118; Mature: 16987

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDPALFRQAMSRFASGVTVVTTIVDAKPYGLTVSAFASLSLDPALAIIAIDKRSPMHD
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHCCCCCEEEEEEECCCCCHHH
LLLQAQAFAINILSNEQESLSRHFAGPQKHDWSEIAAAAGNRSIPLLDGALANMECGLFQ
HHHHHHHHEEEEECCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCEEE
AVDGGDHTIFIGQVEHVTLRDGLPLLYYRGAYHDLVQR
EECCCCCEEEEEEEEEEEECCCCCEEEECCCHHHHHCC
>Mature Secondary Structure 
TLDPALFRQAMSRFASGVTVVTTIVDAKPYGLTVSAFASLSLDPALAIIAIDKRSPMHD
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHCCCCCEEEEEEECCCCCHHH
LLLQAQAFAINILSNEQESLSRHFAGPQKHDWSEIAAAAGNRSIPLLDGALANMECGLFQ
HHHHHHHHEEEEECCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEECCCCCCCCCCEEE
AVDGGDHTIFIGQVEHVTLRDGLPLLYYRGAYHDLVQR
EECCCCCEEEEEEEEEEEECCCCCEEEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]