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The map label for this gene is lacF [H]

Identifier: 159900157

GI number: 159900157

Start: 4578289

End: 4579185

Strand: Reverse

Name: lacF [H]

Synonym: Haur_3640

Alternate gene names: 159900157

Gene position: 4579185-4578289 (Counterclockwise)

Preceding gene: 159900158

Following gene: 159900156

Centisome position: 72.15

GC content: 51.06

Gene sequence:

>897_bases
ATGGGGTCGCAGCTACTTACACCAGCAGAGCGCCGCCACCAATGGCGACGGCGACTAACCCCGTACATGTTTTTACTGCC
AGCATTGTTGATTTTTGGCATCTTTATGCTCTACCCAATTATTGCCTCGTTGCAATTGAGCTTTGAGAGCCAACTCAACC
CTAACAGCGGCTTCAGTCTCGATAACTATCGGCGCTTGTTCGGCGACACGGTGTTTCGTAAAGCACTAATCAACACGGTG
TTTTTGCTGGTATTTCAAGTGCCACTGCAACTTGGCCTCGCCATGGTCTTGGCGGTGTTGCTCAATAGCGCCGTGCTCAA
ATTTCGCACAGCCTTTCGCTTAATTTATTTTCTGCCAGCAATTACCGCCTTATTCGCGGTAGCCTTGATCTTTCGGCTGC
TGCTGAATGATGAAAAAGGCCTGATCAACTATGTGCTCAATGGCTTGGGCGTGCAGCCTGTGCCCTGGCTTACTAATGCT
TGGCCCGCTAAATTCTCGCTGGTTATGGCAATTACCTGGCGCTGGACGGGCTATAACATGGTGATTTATCTCGCCAGTTT
GCAGAGTATCCCAACCGAATTGTACGAGGCGGCAGAGCTTGATGGCGCAGGCGCATGGGCCAAATTCTGGGCGATTACCG
TACCGATGATGCGCCCCACGATTTTGCTAACCACGGTGCTTTCAACCATTGGTACGTTGCAAATTTTCGATGAGCCATAT
CTGCTGACCCGCAGCGGGCCATCTAATGAAACCCTCAGCATGGCCACCTTGCTCTATCGCACGGCCTTCCAAAATGCCGA
GTTTAATTATGCTTCAGCAATTGCCTACGCCATGGTCTTGATCATCGCCGTCTTTTCGCTTTTACAATTTCGCATCGCCC
AGCGCGGGGAGGACTAA

Upstream 100 bases:

>100_bases
GCCGCGAAATCGCCCAATAGGTCATACCCTCACCCCAACCCCTCTCCCGTATGGCGGGAGAGGGGAGTTTGAATTAATGG
CCTTTGCCTAGGAGGGTTCT

Downstream 100 bases:

>100_bases
GCGATGGCAAAAACCAAGCTTTCCGATGAACTTGTGCCTAAGCCCTTTGTGAGCCGCTTCCAGCGTGGCGCGATCTATCT
CTTATTGGTCGTTGGGGCAT

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MGSQLLTPAERRHQWRRRLTPYMFLLPALLIFGIFMLYPIIASLQLSFESQLNPNSGFSLDNYRRLFGDTVFRKALINTV
FLLVFQVPLQLGLAMVLAVLLNSAVLKFRTAFRLIYFLPAITALFAVALIFRLLLNDEKGLINYVLNGLGVQPVPWLTNA
WPAKFSLVMAITWRWTGYNMVIYLASLQSIPTELYEAAELDGAGAWAKFWAITVPMMRPTILLTTVLSTIGTLQIFDEPY
LLTRSGPSNETLSMATLLYRTAFQNAEFNYASAIAYAMVLIIAVFSLLQFRIAQRGED

Sequences:

>Translated_298_residues
MGSQLLTPAERRHQWRRRLTPYMFLLPALLIFGIFMLYPIIASLQLSFESQLNPNSGFSLDNYRRLFGDTVFRKALINTV
FLLVFQVPLQLGLAMVLAVLLNSAVLKFRTAFRLIYFLPAITALFAVALIFRLLLNDEKGLINYVLNGLGVQPVPWLTNA
WPAKFSLVMAITWRWTGYNMVIYLASLQSIPTELYEAAELDGAGAWAKFWAITVPMMRPTILLTTVLSTIGTLQIFDEPY
LLTRSGPSNETLSMATLLYRTAFQNAEFNYASAIAYAMVLIIAVFSLLQFRIAQRGED
>Mature_297_residues
GSQLLTPAERRHQWRRRLTPYMFLLPALLIFGIFMLYPIIASLQLSFESQLNPNSGFSLDNYRRLFGDTVFRKALINTVF
LLVFQVPLQLGLAMVLAVLLNSAVLKFRTAFRLIYFLPAITALFAVALIFRLLLNDEKGLINYVLNGLGVQPVPWLTNAW
PAKFSLVMAITWRWTGYNMVIYLASLQSIPTELYEAAELDGAGAWAKFWAITVPMMRPTILLTTVLSTIGTLQIFDEPYL
LTRSGPSNETLSMATLLYRTAFQNAEFNYASAIAYAMVLIIAVFSLLQFRIAQRGED

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787570, Length=272, Percent_Identity=31.25, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789861, Length=297, Percent_Identity=28.2828282828283, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1790465, Length=246, Percent_Identity=29.2682926829268, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33712; Mature: 33581

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSQLLTPAERRHQWRRRLTPYMFLLPALLIFGIFMLYPIIASLQLSFESQLNPNSGFSL
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
DNYRRLFGDTVFRKALINTVFLLVFQVPLQLGLAMVLAVLLNSAVLKFRTAFRLIYFLPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITALFAVALIFRLLLNDEKGLINYVLNGLGVQPVPWLTNAWPAKFSLVMAITWRWTGYNM
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCHH
VIYLASLQSIPTELYEAAELDGAGAWAKFWAITVPMMRPTILLTTVLSTIGTLQIFDEPY
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCE
LLTRSGPSNETLSMATLLYRTAFQNAEFNYASAIAYAMVLIIAVFSLLQFRIAQRGED
EEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GSQLLTPAERRHQWRRRLTPYMFLLPALLIFGIFMLYPIIASLQLSFESQLNPNSGFSL
CCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
DNYRRLFGDTVFRKALINTVFLLVFQVPLQLGLAMVLAVLLNSAVLKFRTAFRLIYFLPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITALFAVALIFRLLLNDEKGLINYVLNGLGVQPVPWLTNAWPAKFSLVMAITWRWTGYNM
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEEEEEEECCCHH
VIYLASLQSIPTELYEAAELDGAGAWAKFWAITVPMMRPTILLTTVLSTIGTLQIFDEPY
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCE
LLTRSGPSNETLSMATLLYRTAFQNAEFNYASAIAYAMVLIIAVFSLLQFRIAQRGED
EEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]