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The map label for this gene is yitJ [H]

Identifier: 159900149

GI number: 159900149

Start: 4565990

End: 4567843

Strand: Direct

Name: yitJ [H]

Synonym: Haur_3632

Alternate gene names: 159900149

Gene position: 4565990-4567843 (Clockwise)

Preceding gene: 159900148

Following gene: 159900159

Centisome position: 71.94

GC content: 52.16

Gene sequence:

>1854_bases
ATGGCAAATCCATTTTTGGAACAACTAGGGCAGCGAGCCTTACTATGTGATGGCGCGATGGGTACGCAACTGTATGGTCG
CGGCATCGATTTTGACGAGTGCTTCGATGCCTTGAATCTGACCCAGCCAGATGTCGTGCGTGAAATTCACCAGAGTTATA
TTGAGGCTGGGGCCGATATTATTGAAACTAATACCTACGGCGCAAATCGCTTCAAATTGGAGCCATTTGGCTTGGCCGAT
AAAGTCCGCCAAATTAATCATCGTGGCATGAAGCTGGCCCGCGAAGCCCGCGAAATAGCTGGCACCAACACCTTAATTGC
CGGTGCGGTTGGCCCTTTGGGTGTTTTATTACAACCCTATGGACCCCTAACCGAGCAAGCAGCCCACGAAGCCTTTGCCG
AACAAATTGGCACCTTGCTGGAGCAGGGCGCTGATCTGTTGATGTTCGAGACATTTAGCGACTTGCGCGAGATGCTGATT
GCCGTCAAAGCTGCGAAGCAAGTTGGCGATTTGCCAATCGTGGCGCAGATGACCTTTGCCGAAGATGGCCGCACGGTGCT
GGGCAACACGCCTGAGGAAGTGGTGCGCAAATTGGTTGAGCTGGGCGTGGCAGTAGTTGGGGTTAATTGTTCGGTTGGCT
CGCAACGGGTTTTTCGCGTGGTACAAAGCATGCGAGCCGTCAATCCGACGATTCCGATCTCAGCACAACCAAATGCTGGC
TGGCCAACCGAGCGCCATAACCGCGTGTTTTATCCGTCGTCGCCTGAATATATGGCCGATTATGCCCGCCGCATGGTCGA
AGAATTGAATGTGCAAATTGTTGGCGGCTGTTGTGGCACTACTCCGCAGCATATTAGCAACATGCACAGCGCCTTGCAGG
ATCTGAATCCAGCTAGCACGCTGAATATTACGATTGTTGATGATGAAGTGCCAAGCGTCCAATTGCCCAGCAAGAGCGCC
GAAACGACCCAACTTGGCCGCATGTTGGCCGATGGTGCGTTTGTCACGAGTGTCGAAATCGATCCGCCTAAAGGCCATAA
TCCACGGCGTTGCCTCGAAGGTGCTCGCCAAATGCAGGCCGCTGGGGTCAATTTCATCAATGTTGCTGATAGCCCGATGG
CACGGGTGCGCATGAGTGCCTTGCCCATGTGTAACTTGATTCAGCAACAAGTTGGCATGGAAACGATTCTGCACTTTACC
ACCCGCGACCGCTCATTAATGGGCCTACAAAGCGATTTACTCGGTGCGCATGCCTTGGGTGTGCGCAACATCTTGGCATT
AAAGGGTGATCCGCCCTCGTTTGGAGCCTATCCTGGTACATCGGGGGTATTCGATGTTGATACCATCGGCTTGGTTAAGG
TAATTGCGGGTATGAATAGCGGGGTTGATACGGCTGGCAACGATATTGGCACACCAACCAACTTCTTAATTGGCGTAGCG
CTGAATATCAATGCCGAAGACCCCGATTGGGAAATTGATCGGTTGCATCAAAAAGTAGCAGCCGGTGCGCATTATGCCAT
GACCCAAATTTCATATGATGCAACAGAGCTTGATCGGTTCCTTGATAAGCTTGGCCCACTGCCAATTCCGATTATTTTGG
GCTTGATGCCAGTGCAGAGCTATCGCCATGCCTCATTCTTGCATAACGAAGTGCCAGGCATTACCTTGCCCAAGGATGTG
TTGGAGCGTATGAAAGAAGCTGGGGCCAATGGTCGGGCGGTCGGAGTACAAGTTTCACAAGAAATTTTGGCTGCTGCCTA
CGATCGCATTCAAGGGGTCTACATCATGCCATCGTTTGGGCGCTACGAAATGGCCGCCGAAGTGTTGGAAGTGTTGAAAG
CCGCTAACGCGTGA

Upstream 100 bases:

>100_bases
AAAGCGTATACCTGCAAATGTTAAGCAATATGCTATAGTTACTGGCGATCAATGGGGATCGTGCGACAACAACTTTACCA
ATGGACAAGGAACTAAGGCT

Downstream 100 bases:

>100_bases
GCATGCCCTCGCCCCCTGCCGTATAATGGTAGGTTGTTAACAATGGTTGGGTGGAATTTCCTGTCGATTAATATGCCCCC
ACCCCTGGCCCCTCTCCCGC

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MANPFLEQLGQRALLCDGAMGTQLYGRGIDFDECFDALNLTQPDVVREIHQSYIEAGADIIETNTYGANRFKLEPFGLAD
KVRQINHRGMKLAREAREIAGTNTLIAGAVGPLGVLLQPYGPLTEQAAHEAFAEQIGTLLEQGADLLMFETFSDLREMLI
AVKAAKQVGDLPIVAQMTFAEDGRTVLGNTPEEVVRKLVELGVAVVGVNCSVGSQRVFRVVQSMRAVNPTIPISAQPNAG
WPTERHNRVFYPSSPEYMADYARRMVEELNVQIVGGCCGTTPQHISNMHSALQDLNPASTLNITIVDDEVPSVQLPSKSA
ETTQLGRMLADGAFVTSVEIDPPKGHNPRRCLEGARQMQAAGVNFINVADSPMARVRMSALPMCNLIQQQVGMETILHFT
TRDRSLMGLQSDLLGAHALGVRNILALKGDPPSFGAYPGTSGVFDVDTIGLVKVIAGMNSGVDTAGNDIGTPTNFLIGVA
LNINAEDPDWEIDRLHQKVAAGAHYAMTQISYDATELDRFLDKLGPLPIPIILGLMPVQSYRHASFLHNEVPGITLPKDV
LERMKEAGANGRAVGVQVSQEILAAAYDRIQGVYIMPSFGRYEMAAEVLEVLKAANA

Sequences:

>Translated_617_residues
MANPFLEQLGQRALLCDGAMGTQLYGRGIDFDECFDALNLTQPDVVREIHQSYIEAGADIIETNTYGANRFKLEPFGLAD
KVRQINHRGMKLAREAREIAGTNTLIAGAVGPLGVLLQPYGPLTEQAAHEAFAEQIGTLLEQGADLLMFETFSDLREMLI
AVKAAKQVGDLPIVAQMTFAEDGRTVLGNTPEEVVRKLVELGVAVVGVNCSVGSQRVFRVVQSMRAVNPTIPISAQPNAG
WPTERHNRVFYPSSPEYMADYARRMVEELNVQIVGGCCGTTPQHISNMHSALQDLNPASTLNITIVDDEVPSVQLPSKSA
ETTQLGRMLADGAFVTSVEIDPPKGHNPRRCLEGARQMQAAGVNFINVADSPMARVRMSALPMCNLIQQQVGMETILHFT
TRDRSLMGLQSDLLGAHALGVRNILALKGDPPSFGAYPGTSGVFDVDTIGLVKVIAGMNSGVDTAGNDIGTPTNFLIGVA
LNINAEDPDWEIDRLHQKVAAGAHYAMTQISYDATELDRFLDKLGPLPIPIILGLMPVQSYRHASFLHNEVPGITLPKDV
LERMKEAGANGRAVGVQVSQEILAAAYDRIQGVYIMPSFGRYEMAAEVLEVLKAANA
>Mature_616_residues
ANPFLEQLGQRALLCDGAMGTQLYGRGIDFDECFDALNLTQPDVVREIHQSYIEAGADIIETNTYGANRFKLEPFGLADK
VRQINHRGMKLAREAREIAGTNTLIAGAVGPLGVLLQPYGPLTEQAAHEAFAEQIGTLLEQGADLLMFETFSDLREMLIA
VKAAKQVGDLPIVAQMTFAEDGRTVLGNTPEEVVRKLVELGVAVVGVNCSVGSQRVFRVVQSMRAVNPTIPISAQPNAGW
PTERHNRVFYPSSPEYMADYARRMVEELNVQIVGGCCGTTPQHISNMHSALQDLNPASTLNITIVDDEVPSVQLPSKSAE
TTQLGRMLADGAFVTSVEIDPPKGHNPRRCLEGARQMQAAGVNFINVADSPMARVRMSALPMCNLIQQQVGMETILHFTT
RDRSLMGLQSDLLGAHALGVRNILALKGDPPSFGAYPGTSGVFDVDTIGLVKVIAGMNSGVDTAGNDIGTPTNFLIGVAL
NINAEDPDWEIDRLHQKVAAGAHYAMTQISYDATELDRFLDKLGPLPIPIILGLMPVQSYRHASFLHNEVPGITLPKDVL
ERMKEAGANGRAVGVQVSQEILAAAYDRIQGVYIMPSFGRYEMAAEVLEVLKAANA

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=322, Percent_Identity=30.4347826086957, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI157266337, Length=274, Percent_Identity=25.9124087591241, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI13162290, Length=272, Percent_Identity=26.4705882352941, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI87240000, Length=314, Percent_Identity=28.0254777070064, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1790450, Length=324, Percent_Identity=32.0987654320988, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1786456, Length=310, Percent_Identity=30, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535405, Length=321, Percent_Identity=30.8411214953271, Blast_Score=139, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324982, Length=315, Percent_Identity=21.9047619047619, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322966, Length=309, Percent_Identity=24.2718446601942, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325234, Length=269, Percent_Identity=26.7657992565056, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129603, Length=341, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 66737; Mature: 66606

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50970 HCY ; PS00079 MULTICOPPER_OXIDASE1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPFLEQLGQRALLCDGAMGTQLYGRGIDFDECFDALNLTQPDVVREIHQSYIEAGADI
CCCHHHHHHCCCEEEECCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCE
IETNTYGANRFKLEPFGLADKVRQINHRGMKLAREAREIAGTNTLIAGAVGPLGVLLQPY
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHCCC
GPLTEQAAHEAFAEQIGTLLEQGADLLMFETFSDLREMLIAVKAAKQVGDLPIVAQMTFA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
EDGRTVLGNTPEEVVRKLVELGVAVVGVNCSVGSQRVFRVVQSMRAVNPTIPISAQPNAG
CCCCEECCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
WPTERHNRVFYPSSPEYMADYARRMVEELNVQIVGGCCGTTPQHISNMHSALQDLNPAST
CCCCCCCEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCE
LNITIVDDEVPSVQLPSKSAETTQLGRMLADGAFVTSVEIDPPKGHNPRRCLEGARQMQA
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
AGVNFINVADSPMARVRMSALPMCNLIQQQVGMETILHFTTRDRSLMGLQSDLLGAHALG
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VRNILALKGDPPSFGAYPGTSGVFDVDTIGLVKVIAGMNSGVDTAGNDIGTPTNFLIGVA
HHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEE
LNINAEDPDWEIDRLHQKVAAGAHYAMTQISYDATELDRFLDKLGPLPIPIILGLMPVQS
EEECCCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
YRHASFLHNEVPGITLPKDVLERMKEAGANGRAVGVQVSQEILAAAYDRIQGVYIMPSFG
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCEEEECCCC
RYEMAAEVLEVLKAANA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANPFLEQLGQRALLCDGAMGTQLYGRGIDFDECFDALNLTQPDVVREIHQSYIEAGADI
CCHHHHHHCCCEEEECCCCCCHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCE
IETNTYGANRFKLEPFGLADKVRQINHRGMKLAREAREIAGTNTLIAGAVGPLGVLLQPY
EECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHCCC
GPLTEQAAHEAFAEQIGTLLEQGADLLMFETFSDLREMLIAVKAAKQVGDLPIVAQMTFA
CCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
EDGRTVLGNTPEEVVRKLVELGVAVVGVNCSVGSQRVFRVVQSMRAVNPTIPISAQPNAG
CCCCEECCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
WPTERHNRVFYPSSPEYMADYARRMVEELNVQIVGGCCGTTPQHISNMHSALQDLNPAST
CCCCCCCEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCE
LNITIVDDEVPSVQLPSKSAETTQLGRMLADGAFVTSVEIDPPKGHNPRRCLEGARQMQA
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
AGVNFINVADSPMARVRMSALPMCNLIQQQVGMETILHFTTRDRSLMGLQSDLLGAHALG
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VRNILALKGDPPSFGAYPGTSGVFDVDTIGLVKVIAGMNSGVDTAGNDIGTPTNFLIGVA
HHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEE
LNINAEDPDWEIDRLHQKVAAGAHYAMTQISYDATELDRFLDKLGPLPIPIILGLMPVQS
EEECCCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
YRHASFLHNEVPGITLPKDVLERMKEAGANGRAVGVQVSQEILAAAYDRIQGVYIMPSFG
HHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCEEEECCCC
RYEMAAEVLEVLKAANA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]