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The map label for this gene is rpfG [H]

Identifier: 159900087

GI number: 159900087

Start: 4488588

End: 4490303

Strand: Reverse

Name: rpfG [H]

Synonym: Haur_3570

Alternate gene names: 159900087

Gene position: 4490303-4488588 (Counterclockwise)

Preceding gene: 159900088

Following gene: 159900086

Centisome position: 70.75

GC content: 50.93

Gene sequence:

>1716_bases
ATGACATCGCAGACGGTTGTGCAAAATAGTGCCTTTGAGTTGGAACAAGTGTTGGTCGTGATTGAGCGGGCTGATGCAAT
TGCTAGCAGTATGACCCTCGATCCGTTGCTTGATCGCATGCTGGCCTTGTTGATCGAGATTGCTGGCGGTGAGGCGGGTA
CGCTCTATTTGGTTGATGACGATGAGATCGTCTTTCAAGTTGTCCATGGCGATCCGGCGAGTCAACATTTGGTTGGCATG
CGTTTACCAATTAATATCGGTTTGGTTGGGGCTACCATTCGCGCAGCTCAACCATTGTGGATCGAAGATATCAACGCAGA
CCCACGTTGGGATCGACGGCTAGGCGAGGCCAACAGCCTGCAATTACGCTCGCTACTGTCGTTGCCGCTGCTGCTCGAAG
AACAGGTCGTTGGGGTGATTCAGATTTTCAATCCACAACGCTCTGAGCCGCGCTTGCTCAAACTCTTGGGCAATCGCATG
GCTGCCGAAGTCGAAAAAGCCCGCTTGTTGAAAAAAAGTCAGCAACGTGAATCACGCTTGAGTGCCTTAGTCGAGATTGT
GGGCCATATTGGTTCGACCCTCGACCGCACAACCTTGCTGAATTTGATCATCGATTATGCGCGAATTTTGCTTGATGCCG
AGGCTAGTTCGCTGTTTTTGACCGAGCCAGAATCGGGCGATTTGATTTTGCAGCATTCAACTGGCCATCTGCATGAACGC
GTTCAGTCGGTGCGTTTGGCCAAAGGTCGTGGCATCGCAGGCTACGTCGCTGAAACTGGCGAGACAATCTGCGTTGGCGA
TGTCAAGCGCGATCAACGCCACGATGGTAGTGTTGATCAGATGACTGGCTTTGAGACCCGTTCAATTTTGGCTGTGCCAT
TGCGCACTCGGTGCATTACTTTTGGCCGTGAACGAGCGCAATCCGAGGAACATCTGATTGGCTGCATCGAGGTTTTGAAT
AAACGTGATGGCCGTTTCGATGATGATGATAAATTGTTGTTGCAAACGTTGGCGCGGCAAGCTGCAACTGTGCTCGAAAT
TGCCGATTTGTATGCTGATGTCAACGAGCTATTTCTCGATGTTATTCAAGCCTTGACAGCGGCGATTGATGCCAAAGACC
CATATACCGAAGGCCATTCCAAGCGGGTTTCGGAATTTGCCGTGGCGATTGCTGAGGAATTAGAGCTAGAGCCAAGTTTT
ATTCATCATCTACGCATTGGCGCGATTTTGCACGATGTTGGCAAAATTGGCATTAGCGATGGGGTGCTGAAGAAACCTGG
GCGCTTGACCGACGATGAATTTGGCGAGATGAAATCGCATCCGGAAATTGGCGAGCGAATTCTCGGCCATATTCGCATGT
TGCAAACCGAGTTGCCTGTGATTATTCAACATCACGAGCGACTTGATGGTAGCGGCTACCCCTATGGCTTAGCCAAAGGC
CAAATTTCACTCGGCGGACGGATTGTTGCCGTGGCCGATGCCTTTGATGCGATGACCTCTGACCGACCGTATCGCAGAGG
CTTACCAACTGAAGAAGCGTTTCGACGTTTGCGGGCTGGAGCCGACACAGAATTTGATGCTGTCTGTGTTGAAGCGCTCT
GGCAAGCATTAAATCGTGGGCGAGTTCGCCCTCAACGTTTGCGTGATGATATTGGTGACCATCGAATTATCAACGATGCA
ACGCCATTTCGCACGCCACTAACACCATCGGCTTGA

Upstream 100 bases:

>100_bases
CAAGCTAAACCGCGCTCGCAGCGCCGGTATCTGGCTCAGGCTTCAATGCGCTAGCCGCTCAGCCGCCTGCGAGTGCTACC
CGACACAGTGGAGCATGAGT

Downstream 100 bases:

>100_bases
GCAGGCTGGGTTTTCGGGCATTTGCGCGGGCTATGGTGGGTTTGTTGGTCAACAGCATGGCTGTTTGGCCTGAATTTAGT
CTGATGAAAGGAAAACTCGT

Product: metal dependent phosphohydrolase

Products: pyruvate; proteinN-pros-phosphohistidine [C]

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTSQTVVQNSAFELEQVLVVIERADAIASSMTLDPLLDRMLALLIEIAGGEAGTLYLVDDDEIVFQVVHGDPASQHLVGM
RLPINIGLVGATIRAAQPLWIEDINADPRWDRRLGEANSLQLRSLLSLPLLLEEQVVGVIQIFNPQRSEPRLLKLLGNRM
AAEVEKARLLKKSQQRESRLSALVEIVGHIGSTLDRTTLLNLIIDYARILLDAEASSLFLTEPESGDLILQHSTGHLHER
VQSVRLAKGRGIAGYVAETGETICVGDVKRDQRHDGSVDQMTGFETRSILAVPLRTRCITFGRERAQSEEHLIGCIEVLN
KRDGRFDDDDKLLLQTLARQAATVLEIADLYADVNELFLDVIQALTAAIDAKDPYTEGHSKRVSEFAVAIAEELELEPSF
IHHLRIGAILHDVGKIGISDGVLKKPGRLTDDEFGEMKSHPEIGERILGHIRMLQTELPVIIQHHERLDGSGYPYGLAKG
QISLGGRIVAVADAFDAMTSDRPYRRGLPTEEAFRRLRAGADTEFDAVCVEALWQALNRGRVRPQRLRDDIGDHRIINDA
TPFRTPLTPSA

Sequences:

>Translated_571_residues
MTSQTVVQNSAFELEQVLVVIERADAIASSMTLDPLLDRMLALLIEIAGGEAGTLYLVDDDEIVFQVVHGDPASQHLVGM
RLPINIGLVGATIRAAQPLWIEDINADPRWDRRLGEANSLQLRSLLSLPLLLEEQVVGVIQIFNPQRSEPRLLKLLGNRM
AAEVEKARLLKKSQQRESRLSALVEIVGHIGSTLDRTTLLNLIIDYARILLDAEASSLFLTEPESGDLILQHSTGHLHER
VQSVRLAKGRGIAGYVAETGETICVGDVKRDQRHDGSVDQMTGFETRSILAVPLRTRCITFGRERAQSEEHLIGCIEVLN
KRDGRFDDDDKLLLQTLARQAATVLEIADLYADVNELFLDVIQALTAAIDAKDPYTEGHSKRVSEFAVAIAEELELEPSF
IHHLRIGAILHDVGKIGISDGVLKKPGRLTDDEFGEMKSHPEIGERILGHIRMLQTELPVIIQHHERLDGSGYPYGLAKG
QISLGGRIVAVADAFDAMTSDRPYRRGLPTEEAFRRLRAGADTEFDAVCVEALWQALNRGRVRPQRLRDDIGDHRIINDA
TPFRTPLTPSA
>Mature_570_residues
TSQTVVQNSAFELEQVLVVIERADAIASSMTLDPLLDRMLALLIEIAGGEAGTLYLVDDDEIVFQVVHGDPASQHLVGMR
LPINIGLVGATIRAAQPLWIEDINADPRWDRRLGEANSLQLRSLLSLPLLLEEQVVGVIQIFNPQRSEPRLLKLLGNRMA
AEVEKARLLKKSQQRESRLSALVEIVGHIGSTLDRTTLLNLIIDYARILLDAEASSLFLTEPESGDLILQHSTGHLHERV
QSVRLAKGRGIAGYVAETGETICVGDVKRDQRHDGSVDQMTGFETRSILAVPLRTRCITFGRERAQSEEHLIGCIEVLNK
RDGRFDDDDKLLLQTLARQAATVLEIADLYADVNELFLDVIQALTAAIDAKDPYTEGHSKRVSEFAVAIAEELELEPSFI
HHLRIGAILHDVGKIGISDGVLKKPGRLTDDEFGEMKSHPEIGERILGHIRMLQTELPVIIQHHERLDGSGYPYGLAKGQ
ISLGGRIVAVADAFDAMTSDRPYRRGLPTEEAFRRLRAGADTEFDAVCVEALWQALNRGRVRPQRLRDDIGDHRIINDAT
PFRTPLTPSA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Homo sapiens, GI116536085, Length=197, Percent_Identity=28.9340101522843, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI61744432, Length=184, Percent_Identity=31.5217391304348, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI61744430, Length=184, Percent_Identity=31.5217391304348, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI5729972, Length=289, Percent_Identity=25.6055363321799, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI61744435, Length=184, Percent_Identity=31.5217391304348, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194595488, Length=289, Percent_Identity=25.6055363321799, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI4505657, Length=350, Percent_Identity=24, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI226246591, Length=350, Percent_Identity=24, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI219802706, Length=350, Percent_Identity=24, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI116536087, Length=315, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193202333, Length=158, Percent_Identity=32.2784810126582, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161076955, Length=183, Percent_Identity=28.9617486338798, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI161076953, Length=183, Percent_Identity=28.9617486338798, Blast_Score=76, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: 2.7.3.9 [C]

Molecular weight: Translated: 63229; Mature: 63098

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQTVVQNSAFELEQVLVVIERADAIASSMTLDPLLDRMLALLIEIAGGEAGTLYLVDD
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DEIVFQVVHGDPASQHLVGMRLPINIGLVGATIRAAQPLWIEDINADPRWDRRLGEANSL
CCEEEEEECCCCCCCCEEEEECCEEEEEEEHHHHHCCCCEEEECCCCCCHHHHCCCCCHH
QLRSLLSLPLLLEEQVVGVIQIFNPQRSEPRLLKLLGNRMAAEVEKARLLKKSQQRESRL
HHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALVEIVGHIGSTLDRTTLLNLIIDYARILLDAEASSLFLTEPESGDLILQHSTGHLHER
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCHHHHH
VQSVRLAKGRGIAGYVAETGETICVGDVKRDQRHDGSVDQMTGFETRSILAVPLRTRCIT
HHHHHHHCCCCCEEEHCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCEEEECHHHHHHH
FGRERAQSEEHLIGCIEVLNKRDGRFDDDDKLLLQTLARQAATVLEIADLYADVNELFLD
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIQALTAAIDAKDPYTEGHSKRVSEFAVAIAEELELEPSFIHHLRIGAILHDVGKIGISD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
GVLKKPGRLTDDEFGEMKSHPEIGERILGHIRMLQTELPVIIQHHERLDGSGYPYGLAKG
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
QISLGGRIVAVADAFDAMTSDRPYRRGLPTEEAFRRLRAGADTEFDAVCVEALWQALNRG
CEECCCEEEEEEHHHHHHCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
RVRPQRLRDDIGDHRIINDATPFRTPLTPSA
CCCHHHHHHCCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TSQTVVQNSAFELEQVLVVIERADAIASSMTLDPLLDRMLALLIEIAGGEAGTLYLVDD
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DEIVFQVVHGDPASQHLVGMRLPINIGLVGATIRAAQPLWIEDINADPRWDRRLGEANSL
CCEEEEEECCCCCCCCEEEEECCEEEEEEEHHHHHCCCCEEEECCCCCCHHHHCCCCCHH
QLRSLLSLPLLLEEQVVGVIQIFNPQRSEPRLLKLLGNRMAAEVEKARLLKKSQQRESRL
HHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALVEIVGHIGSTLDRTTLLNLIIDYARILLDAEASSLFLTEPESGDLILQHSTGHLHER
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCHHHHH
VQSVRLAKGRGIAGYVAETGETICVGDVKRDQRHDGSVDQMTGFETRSILAVPLRTRCIT
HHHHHHHCCCCCEEEHCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCEEEECHHHHHHH
FGRERAQSEEHLIGCIEVLNKRDGRFDDDDKLLLQTLARQAATVLEIADLYADVNELFLD
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIQALTAAIDAKDPYTEGHSKRVSEFAVAIAEELELEPSFIHHLRIGAILHDVGKIGISD
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
GVLKKPGRLTDDEFGEMKSHPEIGERILGHIRMLQTELPVIIQHHERLDGSGYPYGLAKG
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
QISLGGRIVAVADAFDAMTSDRPYRRGLPTEEAFRRLRAGADTEFDAVCVEALWQALNRG
CEECCCEEEEEEHHHHHHCCCCCHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
RVRPQRLRDDIGDHRIINDATPFRTPLTPSA
CCCHHHHHHCCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.195 {phosphoenolpyruvate}} 0.0105 {histidine-containing} [C]

Substrates: Phosphoenolpyruvate; protein histidine [C]

Specific reaction: Phosphoenolpyruvate + protein histidine = pyruvate + proteinN-pros-phosphohistidine [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Hydroxy -3-butenoic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]