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The map label for this gene is cphA [H]

Identifier: 159900082

GI number: 159900082

Start: 4481447

End: 4483063

Strand: Direct

Name: cphA [H]

Synonym: Haur_3565

Alternate gene names: 159900082

Gene position: 4481447-4483063 (Clockwise)

Preceding gene: 159900081

Following gene: 159900083

Centisome position: 70.61

GC content: 53.31

Gene sequence:

>1617_bases
ATGCTGGAGTTTCGTGATATTCGGGCCTTGGAAGGGCCAAATGGCTATGATCATGCCCCGGTTGCTTTAGTTCGGCTGGT
TGGCGAAACTGTGGAGATCGATGCTTGGCTCAAGCAAGTGGCGCTGGCGTGTGCCAACTATGGCATTGCCCATACTGAGC
CTGTTTTACAACAACACCCTAGTTTGGGTGAATATGCGATTACTTGGCGCACCGCCACGCCCGAAAGTTTGGCTCTGCTG
CTTGAGCAATTGGCGGGCACGGATGCAGCCGCTAGCTCGGCTGAAATTCAGGCTATGTTGGAGCAGGAGCAAATTCCAAA
CCCATTGCACGATTTAGCCAAGCAACACTTGCCACTTTGGCGCGAGGCTGAAGCATGGTGGGTTGGCTATGGTCGTTATC
GCCAAAACGCTGCTCAATTCGACCTTAGCACGTACCAAACCTTGCCAATTATTGCGATTAGCGGTACTAACGGCAAAACC
ACCACGACCCGCTTGATCGATTTTAGCCTGCGCACGGCGGGCTACCAAACAGGCCGCACCGATACCGACGGCGTTTGGAT
TAACAACCAAGCAATCGAGCAAGGCGATTGGACAGGTTTTGGTGGAGCACAACGGGTGCTGAGCCATCCAGCGGTCGAGG
TGGCTGTGTTGGAAACTGCTCGCGGTGGTTTATTGCGCCGTGGCTTGGCCTTCAACCATTGCGAGATTGCGGTTTTAACC
AATGTTGCCGACGATCATCTGCCAGATCGCGGTTTGGCAACCGTCGCCGAAATGGCTCATTTCAAGGCCACCATCGTGCG
AGCCGCCCAAAAAGCCGTTATTTTGAATGCTGATGATCCAGTGTTAGTTGAGGTGGTTGCCCCTCAAGCAACAGCGCCAA
TTATCTGGTTTAGTTTGCAAGCCACCAATCCCTTGATTGTGCAAGCGCAACAGAACCAACAAGCCTGTTTGTATCTCGAT
GCTAACCAATTAATCCTGGCCAGTGCTGGCCAAACTCAAGCATTAATTGCGGTGGCTGAGTTGCCCTTGAGCTTTGGCGG
AGTGGCGCAGCATAATATCGCCAATGCTTTGGCTGCCGCTGCCGCACTGTTTGCGTTTGGATTAAGCGCTGGCCAGATTG
CGCTTGGCCTCGCCGGATTTCAACCAAACGCCGAGCATAATCCAGGCCGTTTGAATCAATTTCAACGCGATGGCATCACC
TTGTTAATTGATTATGCGCACAATAGCGATGGGCTGCGGGTATTATTAGCCAGCGCCGCGCCCCTTCGCGCCGCCAATCG
CCATATTGCCATGGTGTTCAGTGGCACTGGCGATCGTACCGACCAACAAATTCAAGAACAAATGACGATCATCGCTCAGC
ATGTCGATCGGTTGATCCTCAAACGTGATCCATGGTTTTTGCGTGGGCGCGAACCAGGCGTTTTAGAGCCATTGATGATC
GCTGCGGCAACTGCTGCTGGCCTTGATCCTGCCGCAATCGCTGAGCTAGAGGGCGACGAAGCTGCGTTTAATGAGCTGAC
CCAACCAGCCAAGGCGGGCGATGTGCTGATTTGGATTGTTTATAAAGATCGGGCTAGCAAAATTGCCATCGCCCAAGCGT
GGGAGCGCAACCCATGA

Upstream 100 bases:

>100_bases
AATGCTTAGGCTGTGATTGAGTAGCAGCAAATCAATTGCATTTTTCAGCTGTCCATGTACCATTAAGCCTAGATTCGTGC
TGAATTAAGGTTGGTGGCCT

Downstream 100 bases:

>100_bases
GCGAAGCCATGAGCGAGTCTGTAAGCATCCAAGAGGAATACCTGTGGAATAGCGCCCGTGAGGATCTGCCGCCCAAAAAA
CCATTGGTTGAAATTGGTTG

Product: Mur ligase middle domain-containing protein

Products: NA

Alternate protein names: Cyanophycin synthase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MLEFRDIRALEGPNGYDHAPVALVRLVGETVEIDAWLKQVALACANYGIAHTEPVLQQHPSLGEYAITWRTATPESLALL
LEQLAGTDAAASSAEIQAMLEQEQIPNPLHDLAKQHLPLWREAEAWWVGYGRYRQNAAQFDLSTYQTLPIIAISGTNGKT
TTTRLIDFSLRTAGYQTGRTDTDGVWINNQAIEQGDWTGFGGAQRVLSHPAVEVAVLETARGGLLRRGLAFNHCEIAVLT
NVADDHLPDRGLATVAEMAHFKATIVRAAQKAVILNADDPVLVEVVAPQATAPIIWFSLQATNPLIVQAQQNQQACLYLD
ANQLILASAGQTQALIAVAELPLSFGGVAQHNIANALAAAAALFAFGLSAGQIALGLAGFQPNAEHNPGRLNQFQRDGIT
LLIDYAHNSDGLRVLLASAAPLRAANRHIAMVFSGTGDRTDQQIQEQMTIIAQHVDRLILKRDPWFLRGREPGVLEPLMI
AAATAAGLDPAAIAELEGDEAAFNELTQPAKAGDVLIWIVYKDRASKIAIAQAWERNP

Sequences:

>Translated_538_residues
MLEFRDIRALEGPNGYDHAPVALVRLVGETVEIDAWLKQVALACANYGIAHTEPVLQQHPSLGEYAITWRTATPESLALL
LEQLAGTDAAASSAEIQAMLEQEQIPNPLHDLAKQHLPLWREAEAWWVGYGRYRQNAAQFDLSTYQTLPIIAISGTNGKT
TTTRLIDFSLRTAGYQTGRTDTDGVWINNQAIEQGDWTGFGGAQRVLSHPAVEVAVLETARGGLLRRGLAFNHCEIAVLT
NVADDHLPDRGLATVAEMAHFKATIVRAAQKAVILNADDPVLVEVVAPQATAPIIWFSLQATNPLIVQAQQNQQACLYLD
ANQLILASAGQTQALIAVAELPLSFGGVAQHNIANALAAAAALFAFGLSAGQIALGLAGFQPNAEHNPGRLNQFQRDGIT
LLIDYAHNSDGLRVLLASAAPLRAANRHIAMVFSGTGDRTDQQIQEQMTIIAQHVDRLILKRDPWFLRGREPGVLEPLMI
AAATAAGLDPAAIAELEGDEAAFNELTQPAKAGDVLIWIVYKDRASKIAIAQAWERNP
>Mature_538_residues
MLEFRDIRALEGPNGYDHAPVALVRLVGETVEIDAWLKQVALACANYGIAHTEPVLQQHPSLGEYAITWRTATPESLALL
LEQLAGTDAAASSAEIQAMLEQEQIPNPLHDLAKQHLPLWREAEAWWVGYGRYRQNAAQFDLSTYQTLPIIAISGTNGKT
TTTRLIDFSLRTAGYQTGRTDTDGVWINNQAIEQGDWTGFGGAQRVLSHPAVEVAVLETARGGLLRRGLAFNHCEIAVLT
NVADDHLPDRGLATVAEMAHFKATIVRAAQKAVILNADDPVLVEVVAPQATAPIIWFSLQATNPLIVQAQQNQQACLYLD
ANQLILASAGQTQALIAVAELPLSFGGVAQHNIANALAAAAALFAFGLSAGQIALGLAGFQPNAEHNPGRLNQFQRDGIT
LLIDYAHNSDGLRVLLASAAPLRAANRHIAMVFSGTGDRTDQQIQEQMTIIAQHVDRLILKRDPWFLRGREPGVLEPLMI
AAATAAGLDPAAIAELEGDEAAFNELTQPAKAGDVLIWIVYKDRASKIAIAQAWERNP

Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011810
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]

EC number: =6.3.2.29; =6.3.2.30 [H]

Molecular weight: Translated: 58081; Mature: 58081

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFRDIRALEGPNGYDHAPVALVRLVGETVEIDAWLKQVALACANYGIAHTEPVLQQHP
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHCC
SLGEYAITWRTATPESLALLLEQLAGTDAAASSAEIQAMLEQEQIPNPLHDLAKQHLPLW
CCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
REAEAWWVGYGRYRQNAAQFDLSTYQTLPIIAISGTNGKTTTTRLIDFSLRTAGYQTGRT
CCCCEEEEECHHHHCCHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEEEHHHCCCCCCCC
DTDGVWINNQAIEQGDWTGFGGAQRVLSHPAVEVAVLETARGGLLRRGLAFNHCEIAVLT
CCCCEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEHHCCCHHHCCCCCCCEEEEEEE
NVADDHLPDRGLATVAEMAHFKATIVRAAQKAVILNADDPVLVEVVAPQATAPIIWFSLQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCEEEEEEE
ATNPLIVQAQQNQQACLYLDANQLILASAGQTQALIAVAELPLSFGGVAQHNIANALAAA
CCCCEEEEECCCCCEEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
AALFAFGLSAGQIALGLAGFQPNAEHNPGRLNQFQRDGITLLIDYAHNSDGLRVLLASAA
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCEEEEEEECCCCCCEEEEEECCC
PLRAANRHIAMVFSGTGDRTDQQIQEQMTIIAQHVDRLILKRDPWFLRGREPGVLEPLMI
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
AAATAAGLDPAAIAELEGDEAAFNELTQPAKAGDVLIWIVYKDRASKIAIAQAWERNP
HHHHHCCCCHHHHEECCCCHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEHHCCCCC
>Mature Secondary Structure
MLEFRDIRALEGPNGYDHAPVALVRLVGETVEIDAWLKQVALACANYGIAHTEPVLQQHP
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCHHHHHCC
SLGEYAITWRTATPESLALLLEQLAGTDAAASSAEIQAMLEQEQIPNPLHDLAKQHLPLW
CCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCC
REAEAWWVGYGRYRQNAAQFDLSTYQTLPIIAISGTNGKTTTTRLIDFSLRTAGYQTGRT
CCCCEEEEECHHHHCCHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEEEHHHCCCCCCCC
DTDGVWINNQAIEQGDWTGFGGAQRVLSHPAVEVAVLETARGGLLRRGLAFNHCEIAVLT
CCCCEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEHHCCCHHHCCCCCCCEEEEEEE
NVADDHLPDRGLATVAEMAHFKATIVRAAQKAVILNADDPVLVEVVAPQATAPIIWFSLQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCCEEEEEEE
ATNPLIVQAQQNQQACLYLDANQLILASAGQTQALIAVAELPLSFGGVAQHNIANALAAA
CCCCEEEEECCCCCEEEEEECCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
AALFAFGLSAGQIALGLAGFQPNAEHNPGRLNQFQRDGITLLIDYAHNSDGLRVLLASAA
HHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHCCCCEEEEEEECCCCCCEEEEEECCC
PLRAANRHIAMVFSGTGDRTDQQIQEQMTIIAQHVDRLILKRDPWFLRGREPGVLEPLMI
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHH
AAATAAGLDPAAIAELEGDEAAFNELTQPAKAGDVLIWIVYKDRASKIAIAQAWERNP
HHHHHCCCCHHHHEECCCCHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]