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The map label for this gene is proS

Identifier: 159900080

GI number: 159900080

Start: 4478386

End: 4480086

Strand: Direct

Name: proS

Synonym: Haur_3563

Alternate gene names: 159900080

Gene position: 4478386-4480086 (Clockwise)

Preceding gene: 159900074

Following gene: 159900081

Centisome position: 70.56

GC content: 52.62

Gene sequence:

>1701_bases
ATGCGGATGAGTAGTGGTTTCGGGCGCACCTTGCGCGAGGCTCCAAGCGAAGCAGAATTAGCTGCACATCAATTAATTTT
ACGGGCTGGCTTAGCACGGCAATTATTAGCTGGTGGCATGGCGCTGTTGCCACTGGGCATGCGAGTATTTCGGCGGATTG
AAGCAATTATGCATGCTGAATTAGCTGCTATCGGTGCTGGTGAATTTCGCACGCCAGTTGTGCATGCTGCCAGTTTATGG
GAGCAAACCGGACGTTATGCCCAATATGGCGAGGCTATGCTACGCTTCAACAATCGCAATCAACAGGCTTTATTATTTGC
GCCAACCCACGAAGAGGCGGTTGCCGAGCTAGCCCGTCGCGAGGTTGATTCGTATCGCCAACTGCCAAGCCTGCTCTACC
AAATTCATACCAAATATCGCGATGAATTGCGGGTTCGTGGTGGTTTGTTGCGGCTACGCGAATTTACCATGCTTGATGCC
TATTCGCTTGATACCGATTGGGCGGGCTTGGATATGGTTTATGATCGGGTTGCGCTCGCTTTCGAAACGATCTTTGAGCG
GTGTGGCGTGCGTTTTACCGCTGTCGAAGCCGATGGCGGCGAGATGGGCGGCCGTGAACCACGCGAATATATGGCGTTTT
CGAGCAGCGGCGAAGATAGCTTGGTGGTTTGCCCGCTCTGCAGTTACGCCGCCAATAGCGAGGTTGCGGTGCGCGGCCAA
GCTGCTGCCAATGATGATGTCGTACCAGCTATGAGCGAAATCGCCACTCCAGCCTGCACCACGATTGCCGAACTCGCCAC
ATTTTTGCAGGTGAGCGAAGCCCAAACTGCCAAAGCAGTCTTTTTTAACTCAGCCGAAAAGGGCTTGATTTTCGTGGTGG
TGCGCGGTGATCGTGAAGTCAATGAAATTAAATTACGAGCGGCGGCAGGTGTTTCGGCGCTTGAGCCAGCCACGCTTGAG
CAAATTAGCGCGGTTGGGGCAGTCGCAGGCTATGCATCGCCAGTTGGCCTGAGCAATGTTACAGTAATCGCCGATCATTC
GGTGGTTGGCGTTGGTGGCTTGGTTGCTGGAGCCAATCGCACAGGCTATCACTTACAAAACGTCGTGTATGGCCGTGATT
GGCAAGCAACCGTGGTTGCTGATATTGCCAATGTCGAGGAAGGCGATGCTTGCCCTGTCTGTGGCGCAGCTTTGAGCTTG
GAACGGGGCATCGAAATTGGTCATATCTTTAAATTAGGCACTCGTTACACCGAAGCGCTCGGCGCAACTTACCTTGACCC
ACAAGGCCAAGCTCAGCCAATCGTCATGGGTTCGTATGGCATTGGCCTCGAACGTTTGTTGCAAGTCATTATCGAGCAGC
ATCACGATGAAAAAGGCATTGTTTGGCCTGCATCGGTCGCACCATTCGATCTGCATTTGGTGCAACTTGGTGCTAGTGCC
ACGGTCAGCGAGGCCGCTAATCAACTTTATCAACAATTGAGCGAAGCTGGTCTCAGCGTGCTCTACGACGATCGCAATGA
ATCGGCGGGAGTCAAATTTAACGATGCTGATTTATTGGGCATGCCGTTGCGGCTTACGGTTGGCGAACGTGGCCTCAAGC
AAAATGTTGTCGAGTTACGCCAACGAGCAACTGGGGTAGTCGAGACAATCGCGCTTGATCAAGTGGTGAAGAGTATTAAG
AACATAGAGCATAGAGCATAG

Upstream 100 bases:

>100_bases
ATCTGAGCTTCAACTAGGGTGGTACCGCGGTTTTTTTAGCCGTCCCTAGATGCTGTTGCATCTGGGGACGGTTTTTTTAT
TGGCTGCAAGGAGGTCGGTT

Downstream 100 bases:

>100_bases
ATGTAATGGTTTATCGCATGAACAACCGCCAGCGTTCCCTCACCCCCTGACCCCCTCTCCCGCCCGCGAGGCGAGGGGGA
ACCGCTCCAGGAGTGCTCCC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MRMSSGFGRTLREAPSEAELAAHQLILRAGLARQLLAGGMALLPLGMRVFRRIEAIMHAELAAIGAGEFRTPVVHAASLW
EQTGRYAQYGEAMLRFNNRNQQALLFAPTHEEAVAELARREVDSYRQLPSLLYQIHTKYRDELRVRGGLLRLREFTMLDA
YSLDTDWAGLDMVYDRVALAFETIFERCGVRFTAVEADGGEMGGREPREYMAFSSSGEDSLVVCPLCSYAANSEVAVRGQ
AAANDDVVPAMSEIATPACTTIAELATFLQVSEAQTAKAVFFNSAEKGLIFVVVRGDREVNEIKLRAAAGVSALEPATLE
QISAVGAVAGYASPVGLSNVTVIADHSVVGVGGLVAGANRTGYHLQNVVYGRDWQATVVADIANVEEGDACPVCGAALSL
ERGIEIGHIFKLGTRYTEALGATYLDPQGQAQPIVMGSYGIGLERLLQVIIEQHHDEKGIVWPASVAPFDLHLVQLGASA
TVSEAANQLYQQLSEAGLSVLYDDRNESAGVKFNDADLLGMPLRLTVGERGLKQNVVELRQRATGVVETIALDQVVKSIK
NIEHRA

Sequences:

>Translated_566_residues
MRMSSGFGRTLREAPSEAELAAHQLILRAGLARQLLAGGMALLPLGMRVFRRIEAIMHAELAAIGAGEFRTPVVHAASLW
EQTGRYAQYGEAMLRFNNRNQQALLFAPTHEEAVAELARREVDSYRQLPSLLYQIHTKYRDELRVRGGLLRLREFTMLDA
YSLDTDWAGLDMVYDRVALAFETIFERCGVRFTAVEADGGEMGGREPREYMAFSSSGEDSLVVCPLCSYAANSEVAVRGQ
AAANDDVVPAMSEIATPACTTIAELATFLQVSEAQTAKAVFFNSAEKGLIFVVVRGDREVNEIKLRAAAGVSALEPATLE
QISAVGAVAGYASPVGLSNVTVIADHSVVGVGGLVAGANRTGYHLQNVVYGRDWQATVVADIANVEEGDACPVCGAALSL
ERGIEIGHIFKLGTRYTEALGATYLDPQGQAQPIVMGSYGIGLERLLQVIIEQHHDEKGIVWPASVAPFDLHLVQLGASA
TVSEAANQLYQQLSEAGLSVLYDDRNESAGVKFNDADLLGMPLRLTVGERGLKQNVVELRQRATGVVETIALDQVVKSIK
NIEHRA
>Mature_566_residues
MRMSSGFGRTLREAPSEAELAAHQLILRAGLARQLLAGGMALLPLGMRVFRRIEAIMHAELAAIGAGEFRTPVVHAASLW
EQTGRYAQYGEAMLRFNNRNQQALLFAPTHEEAVAELARREVDSYRQLPSLLYQIHTKYRDELRVRGGLLRLREFTMLDA
YSLDTDWAGLDMVYDRVALAFETIFERCGVRFTAVEADGGEMGGREPREYMAFSSSGEDSLVVCPLCSYAANSEVAVRGQ
AAANDDVVPAMSEIATPACTTIAELATFLQVSEAQTAKAVFFNSAEKGLIFVVVRGDREVNEIKLRAAAGVSALEPATLE
QISAVGAVAGYASPVGLSNVTVIADHSVVGVGGLVAGANRTGYHLQNVVYGRDWQATVVADIANVEEGDACPVCGAALSL
ERGIEIGHIFKLGTRYTEALGATYLDPQGQAQPIVMGSYGIGLERLLQVIIEQHHDEKGIVWPASVAPFDLHLVQLGASA
TVSEAANQLYQQLSEAGLSVLYDDRNESAGVKFNDADLLGMPLRLTVGERGLKQNVVELRQRATGVVETIALDQVVKSIK
NIEHRA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=251, Percent_Identity=34.2629482071713, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1786392, Length=576, Percent_Identity=40.4513888888889, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI115532348, Length=214, Percent_Identity=36.4485981308411, Blast_Score=126, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320931, Length=535, Percent_Identity=24.1121495327103, Blast_Score=166, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24656200, Length=215, Percent_Identity=35.3488372093023, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_HERA2 (A9B562)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546327.1
- ProteinModelPortal:   A9B562
- SMR:   A9B562
- GeneID:   5735422
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3563
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- BioCyc:   HAUR316274:HAUR_3563-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 61144; Mature: 61144

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMSSGFGRTLREAPSEAELAAHQLILRAGLARQLLAGGMALLPLGMRVFRRIEAIMHAE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LAAIGAGEFRTPVVHAASLWEQTGRYAQYGEAMLRFNNRNQQALLFAPTHEEAVAELARR
HHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHEECCCCCEEEEEECCCHHHHHHHHHH
EVDSYRQLPSLLYQIHTKYRDELRVRGGLLRLREFTMLDAYSLDTDWAGLDMVYDRVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHH
FETIFERCGVRFTAVEADGGEMGGREPREYMAFSSSGEDSLVVCPLCSYAANSEVAVRGQ
HHHHHHHHCCEEEEEECCCCCCCCCCCHHHHEECCCCCCCEEEEECHHCCCCCCEEEECC
AAANDDVVPAMSEIATPACTTIAELATFLQVSEAQTAKAVFFNSAEKGLIFVVVRGDREV
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCC
NEIKLRAAAGVSALEPATLEQISAVGAVAGYASPVGLSNVTVIADHSVVGVGGLVAGANR
CCEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCEEECCCC
TGYHLQNVVYGRDWQATVVADIANVEEGDACPVCGAALSLERGIEIGHIFKLGTRYTEAL
CCCCHHHEEECCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GATYLDPQGQAQPIVMGSYGIGLERLLQVIIEQHHDEKGIVWPASVAPFDLHLVQLGASA
CCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCH
TVSEAANQLYQQLSEAGLSVLYDDRNESAGVKFNDADLLGMPLRLTVGERGLKQNVVELR
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCEECCCEEEECCCCCHHHHHHHHH
QRATGVVETIALDQVVKSIKNIEHRA
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRMSSGFGRTLREAPSEAELAAHQLILRAGLARQLLAGGMALLPLGMRVFRRIEAIMHAE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LAAIGAGEFRTPVVHAASLWEQTGRYAQYGEAMLRFNNRNQQALLFAPTHEEAVAELARR
HHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHEECCCCCEEEEEECCCHHHHHHHHHH
EVDSYRQLPSLLYQIHTKYRDELRVRGGLLRLREFTMLDAYSLDTDWAGLDMVYDRVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHH
FETIFERCGVRFTAVEADGGEMGGREPREYMAFSSSGEDSLVVCPLCSYAANSEVAVRGQ
HHHHHHHHCCEEEEEECCCCCCCCCCCHHHHEECCCCCCCEEEEECHHCCCCCCEEEECC
AAANDDVVPAMSEIATPACTTIAELATFLQVSEAQTAKAVFFNSAEKGLIFVVVRGDREV
CCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEECCCCC
NEIKLRAAAGVSALEPATLEQISAVGAVAGYASPVGLSNVTVIADHSVVGVGGLVAGANR
CCEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCEEECCCC
TGYHLQNVVYGRDWQATVVADIANVEEGDACPVCGAALSLERGIEIGHIFKLGTRYTEAL
CCCCHHHEEECCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GATYLDPQGQAQPIVMGSYGIGLERLLQVIIEQHHDEKGIVWPASVAPFDLHLVQLGASA
CCCEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCH
TVSEAANQLYQQLSEAGLSVLYDDRNESAGVKFNDADLLGMPLRLTVGERGLKQNVVELR
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCEECCCEEEECCCCCHHHHHHHHH
QRATGVVETIALDQVVKSIKNIEHRA
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA