Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 159900068

GI number: 159900068

Start: 4468259

End: 4469548

Strand: Direct

Name: hemL

Synonym: Haur_3551

Alternate gene names: 159900068

Gene position: 4468259-4469548 (Clockwise)

Preceding gene: 159900067

Following gene: 159900069

Centisome position: 70.4

GC content: 54.11

Gene sequence:

>1290_bases
GTGATCAACGATGCTTCTAGCGCGGCGTTTGAACGCGCCCAAGCACTTTTACCAGGCGGAGTGAATAGCCCAGTGCGGGC
TTTTCGGGGCGTTGGCGGCGTGCCACGCTTTATCGATCATGGCGCAGGAGCCTATCTCTACGACATCGATGGCAATCAAT
ATATCGATTATGTTTTGTCGTGGGGGCCGTTAATTCTGGGCCACGCCTATCCAGCAGTAGTCGAGGCAATTTGTGCCCAA
GCTCAACGTGGCACAAGCTTTGGTGCACCAACCGAGCTTGAAAGCGAATTGGCCGAGTTGGTGATCGCCGCAGTACCAAG
TGTCGAGATGGTGCGCTTTGTTTCGTCGGGCACTGAAGCCGCGATGAGCGCAATTCGTTTGGCGCGGGCTTACACCCAAC
GCGAGAAAATTATCAAATTTGAGGGTTGCTACCACGGCCATGCTGATCCATTTTTGGTGCAAGCTGGCTCAGGTGTGGCA
ACCTTAGGCTTGCCCGATAGCCCAGGCGTTTTGAAAAGCGCTACCAGCAACACCCTGACCGCACCATTTAACGATCTTGA
AGCAGTCGAAGCGCTATTTAAAGCCAATGCTGGGCAAGTTGCCGCCTTGGTAATCGAGCCTGTGGCAGGCAATATGGGCT
TTGTACTGCCACGCGAAGGCTATCTTGCAGGCCTGCGCCAACTTTGCGATCAATACGGGGCATTATTGATTTTCGACGAA
GTAATGACGGGCTTTCGCGTGGCCTACGGTGGAGCACAAGCCTACTTCAACGTGATGCCCGATTTGACCTGCTTGGGCAA
AGTAGTAGGCGGCGGTTTGCCAGCGGCGGCCTATGGCGGACGACGCGAGATTATGCAGATGGTCGCTCCAGCTGGCACAA
TGTATCAAGCTGGCACGCTTTCGGGCAACCCACTGGCGATGGTCGCTGGCATTGTAACTTTACGCGAAATTGCCAAGCCC
GAAGTTTTCGAGCGCTTAACTGGTGTAACTTCGACGCTGTGTCAAGGCTTTTGGAAGGCCGCCTTCAAAAATGGCATTCC
CTTCCAAGCGCATAAAGCTGGCAGTATGTGGGGCTTCTTCTTTGCTGGCGATGAGGTTTATGATTTCACATCGGCCAAGC
GGGCTGATACCGCCATGTTTGGCAAATTCTTCCATGCCATGCTGGAGCAAGGCGTGTATCTTGCGCCGTCGCAATTTGAG
GCCGCCTTTGTCTCAACGGCCCATACCGACGAACTCGTCGCTCAAACGATAAATGCAGCCCAAGCGGCTTTTGCCAGCAT
TCGCAGCTAA

Upstream 100 bases:

>100_bases
CAGGGTTTCTTCGTGCTCTTCGTGTTCTTCGTGGTTTCAAAACCGATCCCCAGCCCCCGATCCCCGATCCCCGAGACATA
ACATATGGAGACAGAAAAAC

Downstream 100 bases:

>100_bases
AATAACAAGCAGCGCCAGTTTAATTAAGCTGGCGCTCGTTTTTCTCAACGGGTCAAATACCAAGCAAATCCTGCAATTAT
TATCACCAAAGCTACTGAAA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MINDASSAAFERAQALLPGGVNSPVRAFRGVGGVPRFIDHGAGAYLYDIDGNQYIDYVLSWGPLILGHAYPAVVEAICAQ
AQRGTSFGAPTELESELAELVIAAVPSVEMVRFVSSGTEAAMSAIRLARAYTQREKIIKFEGCYHGHADPFLVQAGSGVA
TLGLPDSPGVLKSATSNTLTAPFNDLEAVEALFKANAGQVAALVIEPVAGNMGFVLPREGYLAGLRQLCDQYGALLIFDE
VMTGFRVAYGGAQAYFNVMPDLTCLGKVVGGGLPAAAYGGRREIMQMVAPAGTMYQAGTLSGNPLAMVAGIVTLREIAKP
EVFERLTGVTSTLCQGFWKAAFKNGIPFQAHKAGSMWGFFFAGDEVYDFTSAKRADTAMFGKFFHAMLEQGVYLAPSQFE
AAFVSTAHTDELVAQTINAAQAAFASIRS

Sequences:

>Translated_429_residues
MINDASSAAFERAQALLPGGVNSPVRAFRGVGGVPRFIDHGAGAYLYDIDGNQYIDYVLSWGPLILGHAYPAVVEAICAQ
AQRGTSFGAPTELESELAELVIAAVPSVEMVRFVSSGTEAAMSAIRLARAYTQREKIIKFEGCYHGHADPFLVQAGSGVA
TLGLPDSPGVLKSATSNTLTAPFNDLEAVEALFKANAGQVAALVIEPVAGNMGFVLPREGYLAGLRQLCDQYGALLIFDE
VMTGFRVAYGGAQAYFNVMPDLTCLGKVVGGGLPAAAYGGRREIMQMVAPAGTMYQAGTLSGNPLAMVAGIVTLREIAKP
EVFERLTGVTSTLCQGFWKAAFKNGIPFQAHKAGSMWGFFFAGDEVYDFTSAKRADTAMFGKFFHAMLEQGVYLAPSQFE
AAFVSTAHTDELVAQTINAAQAAFASIRS
>Mature_429_residues
MINDASSAAFERAQALLPGGVNSPVRAFRGVGGVPRFIDHGAGAYLYDIDGNQYIDYVLSWGPLILGHAYPAVVEAICAQ
AQRGTSFGAPTELESELAELVIAAVPSVEMVRFVSSGTEAAMSAIRLARAYTQREKIIKFEGCYHGHADPFLVQAGSGVA
TLGLPDSPGVLKSATSNTLTAPFNDLEAVEALFKANAGQVAALVIEPVAGNMGFVLPREGYLAGLRQLCDQYGALLIFDE
VMTGFRVAYGGAQAYFNVMPDLTCLGKVVGGGLPAAAYGGRREIMQMVAPAGTMYQAGTLSGNPLAMVAGIVTLREIAKP
EVFERLTGVTSTLCQGFWKAAFKNGIPFQAHKAGSMWGFFFAGDEVYDFTSAKRADTAMFGKFFHAMLEQGVYLAPSQFE
AAFVSTAHTDELVAQTINAAQAAFASIRS

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=31.6498316498317, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI24119277, Length=352, Percent_Identity=25.5681818181818, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI37574042, Length=371, Percent_Identity=25.6064690026954, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI284507298, Length=215, Percent_Identity=33.4883720930233, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI226442709, Length=350, Percent_Identity=25.4285714285714, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI226442705, Length=371, Percent_Identity=24.7978436657682, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786349, Length=423, Percent_Identity=56.2647754137116, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1789016, Length=329, Percent_Identity=33.434650455927, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787560, Length=335, Percent_Identity=34.6268656716418, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789759, Length=293, Percent_Identity=33.4470989761092, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI145693181, Length=409, Percent_Identity=29.8288508557457, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788044, Length=295, Percent_Identity=32.8813559322034, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786991, Length=292, Percent_Identity=30.1369863013699, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25144271, Length=297, Percent_Identity=29.6296296296296, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71992977, Length=362, Percent_Identity=27.9005524861878, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25144274, Length=148, Percent_Identity=37.1621621621622, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323470, Length=311, Percent_Identity=26.6881028938907, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324432, Length=331, Percent_Identity=26.2839879154079, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21357415, Length=296, Percent_Identity=28.3783783783784, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356575, Length=311, Percent_Identity=29.5819935691318, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_HERA2 (A9B550)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546315.1
- ProteinModelPortal:   A9B550
- SMR:   A9B550
- GeneID:   5735410
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3551
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- ProtClustDB:   PRK00062
- BioCyc:   HAUR316274:HAUR_3551-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45394; Mature: 45394

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDASSAAFERAQALLPGGVNSPVRAFRGVGGVPRFIDHGAGAYLYDIDGNQYIDYVLS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHCCCCEEEEECCCCHHHHHHHH
WGPLILGHAYPAVVEAICAQAQRGTSFGAPTELESELAELVIAAVPSVEMVRFVSSGTEA
HCHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
AMSAIRLARAYTQREKIIKFEGCYHGHADPFLVQAGSGVATLGLPDSPGVLKSATSNTLT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCHHHHCCCCCCC
APFNDLEAVEALFKANAGQVAALVIEPVAGNMGFVLPREGYLAGLRQLCDQYGALLIFDE
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCEEEHHH
VMTGFRVAYGGAQAYFNVMPDLTCLGKVVGGGLPAAAYGGRREIMQMVAPAGTMYQAGTL
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMVAGIVTLREIAKPEVFERLTGVTSTLCQGFWKAAFKNGIPFQAHKAGSMWGFF
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEE
FAGDEVYDFTSAKRADTAMFGKFFHAMLEQGVYLAPSQFEAAFVSTAHTDELVAQTINAA
EECCHHHHHHCHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHCCHHHHHHHHHHHH
QAAFASIRS
HHHHHHHCC
>Mature Secondary Structure
MINDASSAAFERAQALLPGGVNSPVRAFRGVGGVPRFIDHGAGAYLYDIDGNQYIDYVLS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHCCCCEEEEECCCCHHHHHHHH
WGPLILGHAYPAVVEAICAQAQRGTSFGAPTELESELAELVIAAVPSVEMVRFVSSGTEA
HCHHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
AMSAIRLARAYTQREKIIKFEGCYHGHADPFLVQAGSGVATLGLPDSPGVLKSATSNTLT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEECCCCEEEECCCCCCCHHHHCCCCCCC
APFNDLEAVEALFKANAGQVAALVIEPVAGNMGFVLPREGYLAGLRQLCDQYGALLIFDE
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHCCEEEHHH
VMTGFRVAYGGAQAYFNVMPDLTCLGKVVGGGLPAAAYGGRREIMQMVAPAGTMYQAGTL
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMVAGIVTLREIAKPEVFERLTGVTSTLCQGFWKAAFKNGIPFQAHKAGSMWGFF
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEE
FAGDEVYDFTSAKRADTAMFGKFFHAMLEQGVYLAPSQFEAAFVSTAHTDELVAQTINAA
EECCHHHHHHCHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHCCHHHHHHHHHHHH
QAAFASIRS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA