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The map label for this gene is rfaQ [H]

Identifier: 159899948

GI number: 159899948

Start: 4320066

End: 4321205

Strand: Reverse

Name: rfaQ [H]

Synonym: Haur_3431

Alternate gene names: 159899948

Gene position: 4321205-4320066 (Counterclockwise)

Preceding gene: 159899949

Following gene: 159899947

Centisome position: 68.09

GC content: 54.47

Gene sequence:

>1140_bases
ATGAAAGCTTTACTACGTTCGACAATGGTTCGAGGTTTGGCGCTGGCGTTGGCTGGTTTTACCCGCCCAGCACCAGCCCA
ACCTCAACGAATTTTGGTGATTAAGCCCGATCATTTGGGCGATGTTTTACTGTTGACCCCAGCGCTACGGGCCTTGCGCT
ATAGCCAACCGCACGCCCAGATCAGCGTTTTGGTTGGTTCATGGGCTACCCGCCTCTTGGCCGACAATCCTGATCTTGAT
GCGATTGAAACCTGTGAGTTTCCTGGTTTTGTGCGCGGTGCGCAACCCTCGACCTTGGCTCCTTATCGCTTGCTTTGGCG
CGAAGCTGCCCGTTTACGAAGCATGAATTTTGATACAGCCTTGATTGCCCGTGATGATCATTGGTGGGGCGGCTTGTTGG
CGCTTGGGGCTGGCTGTGGCCGCCGAATTGGTTTTGCCCATCCCTTGGTTGCGCCAACCTTAACCAAAGCTCTAGCGTGG
AATAGCAATGAGCATGTTACCAAGCAAGCCTTGGATTTGGTGGCAGCGCTCGGTTCAGATCAACCACAAACCCAAACGTT
GCGGTTTATGCCAAGTGCCGCTGAGCATGCTTGGGCTGAGGCTTGGCTGGCGCAGCATCAGATTCAAAAACCATTGGTGG
CAATTCAGGCTGGCAGTGGCGGCGCAGCTAAATTATGGCCGGCTGAGCGTTGGGCACAGGTCGCTGAACAATTGGCAAAT
CAAGCCCAAATTGTATTAACTGGTGGGCCAGCCGATGCTGTTGATGTGGCAGCAATCAGCCAACAGTTGCAAATTCCCCA
TTTGAATGCAGTTGGTCAGGCCAATTTGGGCCAATTGGCGGCCTTGTTTGGGCGTTGCGCTTTGGTGTTGGGCGTGGATA
ACGGCCCTTTGCATTTGGCCGTGAGTCAATCAACCCCAACCATTCATCTGTTTGGGCCAGGCGATAAGCGGCGTTTTGGG
CCTTGGGGCGACCCAACCCGCCACGTTGTGATCGATGCCGAATTAGCCTGCTCGCCATGTGGTGTGTTGACCCATTGCCC
ACGCCAAACCAAACCCAGCGAGTGCATGACCGCAATTTCCGTCCAGCACGTGATCGGTCACGCCAAACGTCTGCTTGATC
AGGCTGGAACCTCAATTTAG

Upstream 100 bases:

>100_bases
TTTTAAGGTCAAAAATCAGAAGTCAAAAGGCAGAATAGTTGGTGTAACTCGATTATAATAACCAAGGATTTTTGCCTTTT
GATTTTGCCTTTTGATTTCT

Downstream 100 bases:

>100_bases
CAGGCTACGTATCATCAAGGCAATTAATTTGTTTTGAGGAAAAGCGTTATGCAACAGTGGGAATATCATGTTGAACTTGT
GGATATTGGGGGCTTCTGGG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKALLRSTMVRGLALALAGFTRPAPAQPQRILVIKPDHLGDVLLLTPALRALRYSQPHAQISVLVGSWATRLLADNPDLD
AIETCEFPGFVRGAQPSTLAPYRLLWREAARLRSMNFDTALIARDDHWWGGLLALGAGCGRRIGFAHPLVAPTLTKALAW
NSNEHVTKQALDLVAALGSDQPQTQTLRFMPSAAEHAWAEAWLAQHQIQKPLVAIQAGSGGAAKLWPAERWAQVAEQLAN
QAQIVLTGGPADAVDVAAISQQLQIPHLNAVGQANLGQLAALFGRCALVLGVDNGPLHLAVSQSTPTIHLFGPGDKRRFG
PWGDPTRHVVIDAELACSPCGVLTHCPRQTKPSECMTAISVQHVIGHAKRLLDQAGTSI

Sequences:

>Translated_379_residues
MKALLRSTMVRGLALALAGFTRPAPAQPQRILVIKPDHLGDVLLLTPALRALRYSQPHAQISVLVGSWATRLLADNPDLD
AIETCEFPGFVRGAQPSTLAPYRLLWREAARLRSMNFDTALIARDDHWWGGLLALGAGCGRRIGFAHPLVAPTLTKALAW
NSNEHVTKQALDLVAALGSDQPQTQTLRFMPSAAEHAWAEAWLAQHQIQKPLVAIQAGSGGAAKLWPAERWAQVAEQLAN
QAQIVLTGGPADAVDVAAISQQLQIPHLNAVGQANLGQLAALFGRCALVLGVDNGPLHLAVSQSTPTIHLFGPGDKRRFG
PWGDPTRHVVIDAELACSPCGVLTHCPRQTKPSECMTAISVQHVIGHAKRLLDQAGTSI
>Mature_379_residues
MKALLRSTMVRGLALALAGFTRPAPAQPQRILVIKPDHLGDVLLLTPALRALRYSQPHAQISVLVGSWATRLLADNPDLD
AIETCEFPGFVRGAQPSTLAPYRLLWREAARLRSMNFDTALIARDDHWWGGLLALGAGCGRRIGFAHPLVAPTLTKALAW
NSNEHVTKQALDLVAALGSDQPQTQTLRFMPSAAEHAWAEAWLAQHQIQKPLVAIQAGSGGAAKLWPAERWAQVAEQLAN
QAQIVLTGGPADAVDVAAISQQLQIPHLNAVGQANLGQLAALFGRCALVLGVDNGPLHLAVSQSTPTIHLFGPGDKRRFG
PWGDPTRHVVIDAELACSPCGVLTHCPRQTKPSECMTAISVQHVIGHAKRLLDQAGTSI

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=314, Percent_Identity=26.4331210191083, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 40606; Mature: 40606

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALLRSTMVRGLALALAGFTRPAPAQPQRILVIKPDHLGDVLLLTPALRALRYSQPHAQ
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCE
ISVLVGSWATRLLADNPDLDAIETCEFPGFVRGAQPSTLAPYRLLWREAARLRSMNFDTA
EEEEHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LIARDDHWWGGLLALGAGCGRRIGFAHPLVAPTLTKALAWNSNEHVTKQALDLVAALGSD
EEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
QPQTQTLRFMPSAAEHAWAEAWLAQHQIQKPLVAIQAGSGGAAKLWPAERWAQVAEQLAN
CCCCHHEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
QAQIVLTGGPADAVDVAAISQQLQIPHLNAVGQANLGQLAALFGRCALVLGVDNGPLHLA
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCEEEEEECCCCCEEEE
VSQSTPTIHLFGPGDKRRFGPWGDPTRHVVIDAELACSPCGVLTHCPRQTKPSECMTAIS
EECCCCEEEEECCCCCCCCCCCCCCCCEEEEECHHCCCCCCHHHCCCCCCCHHHHHHHHH
VQHVIGHAKRLLDQAGTSI
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKALLRSTMVRGLALALAGFTRPAPAQPQRILVIKPDHLGDVLLLTPALRALRYSQPHAQ
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCE
ISVLVGSWATRLLADNPDLDAIETCEFPGFVRGAQPSTLAPYRLLWREAARLRSMNFDTA
EEEEHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LIARDDHWWGGLLALGAGCGRRIGFAHPLVAPTLTKALAWNSNEHVTKQALDLVAALGSD
EEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
QPQTQTLRFMPSAAEHAWAEAWLAQHQIQKPLVAIQAGSGGAAKLWPAERWAQVAEQLAN
CCCCHHEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
QAQIVLTGGPADAVDVAAISQQLQIPHLNAVGQANLGQLAALFGRCALVLGVDNGPLHLA
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCEEEEEECCCCCEEEE
VSQSTPTIHLFGPGDKRRFGPWGDPTRHVVIDAELACSPCGVLTHCPRQTKPSECMTAIS
EECCCCEEEEECCCCCCCCCCCCCCCCEEEEECHHCCCCCCHHHCCCCCCCHHHHHHHHH
VQHVIGHAKRLLDQAGTSI
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]