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The map label for this gene is alkK [H]

Identifier: 159899865

GI number: 159899865

Start: 4221766

End: 4223394

Strand: Reverse

Name: alkK [H]

Synonym: Haur_3348

Alternate gene names: 159899865

Gene position: 4223394-4221766 (Counterclockwise)

Preceding gene: 159899866

Following gene: 159899860

Centisome position: 66.55

GC content: 52.85

Gene sequence:

>1629_bases
ATGCTACGTGGCCTGATGATGGACTATCCGCTGACCGTTGATCGGTTGCTCGACCACGCTTATAAACTCTACCCCCACAA
ACGCGTGACAACCAAGCAACCTGATGGCTCAATCCACCGCTATTCGTTCGCCGATCTCTACCACCGTGTGAAGCGCTTAG
GCAATGTGCTGCACAAACTTGGCATCAACCAAGGCGATCGCGTCGGCACGTTTGCTTGGAACAATTACCAACATCTTGAA
CTCTACTATGCAATTCCTTGTGCTGGCGCGGTTTGTCATACCTTGAATATTCGGCTTTCAACCGAGCAACTGGCCTATAT
CATCAATCATGCCGAAGATAAGGTGATTTTTGTTGATGCAACCTTGTTGCCATTGTTCTCCAAGCTTGCCGATAATATTC
CTGCGGTCGAAACCATCGTTTTGATCAATGCCCAACCTGGCATCGAAACACCCTTCCCGAATGTGTTGCATTACGAAGAT
TTGATGGCCCAAGTCGAGGCTGAATTCGACTGGCCTGTCACTGACGAGCGCCAAGCGATGGGCTTGTGCTACACCAGCGG
CACAACCGGCAATCCCAAAGGCGTGTTATATAGCCATCGTTCGCTCTATTTGCACACGATGGGCGAAAATCAAGCCACAG
CCTTAGCCTTTACTCCCGATGATATTGTGATGCCCGTCGTGCCTCAGTTTCATGCGATGGCGTGGGGATTGCCCTACTCG
GCCATGTTTGCTGGCGCAGATTTGGTGATGCCTGGCTTGCACTTGAATCCAGTGGCCTTGGCCGATTTGATTGCCGACGA
AAAAATCACCTTCCCCGCTGGTGTGCCGACCATTTGGACGGCGATGTATCAAGAATTACGCGCCAATCCCCGCGATTTTT
CGCATGTGCGCTGTTTGGCAGTTGGTGGTGCAGCTATGCCACGCGGCTTGATCGAAGCCTACGAACGCGATTTCGGCGTG
CCTGTGTTGCATGCTTGGGGCATGACCGAGCTTTCGCCCTTGGGCACAATTTCAAGCTTGCAACCGCAACATCGCCAACT
AAGCGACCACGAACGCTGGGATTTACGTGCCAAACAGGGTTATCCAATCGGCGGAGTCGAATTGCGGATCGTCAATGATG
CTGGCGAGGAATTGCCGTGGGATGGTACAACGGTTGGCGAGTTGCAGGCGCGTGGCCCGTGGGTTACCGCTGGCTATTAC
AAAGTTGAGCCAACCGCCGAGCATTTTACCGCCGATGGTTGGTTTCGCACTGGCGACGTGGCTACAATCAACCACGAAGG
CTATTTGGGAATTACCGACCGCACCAAAGATTTGGTTAAGAGCGGCGGCGAATGGATCTCATCGGTTGAGCTGGAAAATA
CCTTGATGGGCCATGCCAAGGTCACTGAGGCGGCGGTGATCGCGATTCCCGATGAGCGTTGGAGCGAGCGCCCGTTGGCC
TGTGTGGTGCTGACCAAAGATGCAGGCGAGGTTGAGCCGAGTGAACTCTTGGAGTATCTTGAGCCGTTGGTGGCCAAATT
CTGGCTACCTGAACGAGTTGTGTTTGTAAGCGAAATTCCCAAAACCAGCGTGGGCAAATTCGATAAAAAAGTGCTACGTG
CCCGCTATGCCAATGGTGAATTAGGCTAA

Upstream 100 bases:

>100_bases
GCGTTAAATTTCATCCTTCATCCTTTCTGCTCTATGGTATGATGGCGCAAATCGATAGCTGAGAGCCAGCCCCCGTTTTG
GAGTGCAAGGAGGCATTGCA

Downstream 100 bases:

>100_bases
TTAAACAAAAACAGGGCGGATGTTTCATCCGCCCTGTTGATCTATTAATTGGGGTTTAACAGACTGTTTCCCCAAAACAT
GCTTCGAGGAGGCTACGAAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLRGLMMDYPLTVDRLLDHAYKLYPHKRVTTKQPDGSIHRYSFADLYHRVKRLGNVLHKLGINQGDRVGTFAWNNYQHLE
LYYAIPCAGAVCHTLNIRLSTEQLAYIINHAEDKVIFVDATLLPLFSKLADNIPAVETIVLINAQPGIETPFPNVLHYED
LMAQVEAEFDWPVTDERQAMGLCYTSGTTGNPKGVLYSHRSLYLHTMGENQATALAFTPDDIVMPVVPQFHAMAWGLPYS
AMFAGADLVMPGLHLNPVALADLIADEKITFPAGVPTIWTAMYQELRANPRDFSHVRCLAVGGAAMPRGLIEAYERDFGV
PVLHAWGMTELSPLGTISSLQPQHRQLSDHERWDLRAKQGYPIGGVELRIVNDAGEELPWDGTTVGELQARGPWVTAGYY
KVEPTAEHFTADGWFRTGDVATINHEGYLGITDRTKDLVKSGGEWISSVELENTLMGHAKVTEAAVIAIPDERWSERPLA
CVVLTKDAGEVEPSELLEYLEPLVAKFWLPERVVFVSEIPKTSVGKFDKKVLRARYANGELG

Sequences:

>Translated_542_residues
MLRGLMMDYPLTVDRLLDHAYKLYPHKRVTTKQPDGSIHRYSFADLYHRVKRLGNVLHKLGINQGDRVGTFAWNNYQHLE
LYYAIPCAGAVCHTLNIRLSTEQLAYIINHAEDKVIFVDATLLPLFSKLADNIPAVETIVLINAQPGIETPFPNVLHYED
LMAQVEAEFDWPVTDERQAMGLCYTSGTTGNPKGVLYSHRSLYLHTMGENQATALAFTPDDIVMPVVPQFHAMAWGLPYS
AMFAGADLVMPGLHLNPVALADLIADEKITFPAGVPTIWTAMYQELRANPRDFSHVRCLAVGGAAMPRGLIEAYERDFGV
PVLHAWGMTELSPLGTISSLQPQHRQLSDHERWDLRAKQGYPIGGVELRIVNDAGEELPWDGTTVGELQARGPWVTAGYY
KVEPTAEHFTADGWFRTGDVATINHEGYLGITDRTKDLVKSGGEWISSVELENTLMGHAKVTEAAVIAIPDERWSERPLA
CVVLTKDAGEVEPSELLEYLEPLVAKFWLPERVVFVSEIPKTSVGKFDKKVLRARYANGELG
>Mature_542_residues
MLRGLMMDYPLTVDRLLDHAYKLYPHKRVTTKQPDGSIHRYSFADLYHRVKRLGNVLHKLGINQGDRVGTFAWNNYQHLE
LYYAIPCAGAVCHTLNIRLSTEQLAYIINHAEDKVIFVDATLLPLFSKLADNIPAVETIVLINAQPGIETPFPNVLHYED
LMAQVEAEFDWPVTDERQAMGLCYTSGTTGNPKGVLYSHRSLYLHTMGENQATALAFTPDDIVMPVVPQFHAMAWGLPYS
AMFAGADLVMPGLHLNPVALADLIADEKITFPAGVPTIWTAMYQELRANPRDFSHVRCLAVGGAAMPRGLIEAYERDFGV
PVLHAWGMTELSPLGTISSLQPQHRQLSDHERWDLRAKQGYPIGGVELRIVNDAGEELPWDGTTVGELQARGPWVTAGYY
KVEPTAEHFTADGWFRTGDVATINHEGYLGITDRTKDLVKSGGEWISSVELENTLMGHAKVTEAAVIAIPDERWSERPLA
CVVLTKDAGEVEPSELLEYLEPLVAKFWLPERVVFVSEIPKTSVGKFDKKVLRARYANGELG

Specific function: Unknown

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=543, Percent_Identity=28.9134438305709, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI187761343, Length=543, Percent_Identity=28.9134438305709, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI156151445, Length=532, Percent_Identity=27.4436090225564, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=25.531914893617, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=22.8515625, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI58082049, Length=517, Percent_Identity=22.8239845261122, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI122937307, Length=511, Percent_Identity=23.4833659491194, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI157311624, Length=510, Percent_Identity=22.3529411764706, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI157311622, Length=510, Percent_Identity=22.3529411764706, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI13375727, Length=536, Percent_Identity=23.6940298507463, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=523, Percent_Identity=23.3269598470363, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI28416953, Length=546, Percent_Identity=24.7252747252747, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI145693145, Length=501, Percent_Identity=28.3433133732535, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI221142682, Length=530, Percent_Identity=26.6037735849057, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788107, Length=525, Percent_Identity=25.3333333333333, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786810, Length=362, Percent_Identity=30.3867403314917, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1789201, Length=559, Percent_Identity=24.3291592128801, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790505, Length=533, Percent_Identity=25.140712945591, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788595, Length=495, Percent_Identity=22.4242424242424, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786801, Length=442, Percent_Identity=23.7556561085973, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786529, Length=543, Percent_Identity=22.2836095764273, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=509, Percent_Identity=27.3084479371316, Blast_Score=165, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17531443, Length=530, Percent_Identity=25.8490566037736, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17560308, Length=528, Percent_Identity=27.4621212121212, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=26.3969171483622, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=510, Percent_Identity=27.843137254902, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=516, Percent_Identity=27.3255813953488, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=515, Percent_Identity=25.8252427184466, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=561, Percent_Identity=24.777183600713, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=561, Percent_Identity=24.777183600713, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=561, Percent_Identity=24.777183600713, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI25147511, Length=505, Percent_Identity=24.950495049505, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=508, Percent_Identity=25.1968503937008, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=360, Percent_Identity=26.6666666666667, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=509, Percent_Identity=22.3968565815324, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319699, Length=361, Percent_Identity=32.9639889196676, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319264, Length=570, Percent_Identity=22.6315789473684, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324893, Length=519, Percent_Identity=22.9287090558767, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=554, Percent_Identity=23.2851985559567, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355181, Length=528, Percent_Identity=26.3257575757576, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24581924, Length=523, Percent_Identity=27.5334608030593, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18859661, Length=397, Percent_Identity=30.9823677581864, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19922652, Length=512, Percent_Identity=24.21875, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356947, Length=493, Percent_Identity=24.9492900608519, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653035, Length=551, Percent_Identity=23.2304900181488, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648253, Length=543, Percent_Identity=21.5469613259669, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648255, Length=543, Percent_Identity=21.5469613259669, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24656500, Length=536, Percent_Identity=23.5074626865672, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358303, Length=510, Percent_Identity=22.7450980392157, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355225, Length=558, Percent_Identity=22.9390681003584, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648260, Length=507, Percent_Identity=24.65483234714, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161076582, Length=561, Percent_Identity=22.1033868092692, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356441, Length=508, Percent_Identity=23.4251968503937, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648257, Length=552, Percent_Identity=22.8260869565217, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62472339, Length=583, Percent_Identity=23.4991423670669, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667955, Length=564, Percent_Identity=23.4042553191489, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281365686, Length=511, Percent_Identity=20.7436399217221, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550496, Length=555, Percent_Identity=22.1621621621622, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552801, Length=555, Percent_Identity=21.981981981982, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24667959, Length=393, Percent_Identity=24.1730279898219, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 60399; Mature: 60399

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGLMMDYPLTVDRLLDHAYKLYPHKRVTTKQPDGSIHRYSFADLYHRVKRLGNVLHKL
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
GINQGDRVGTFAWNNYQHLELYYAIPCAGAVCHTLNIRLSTEQLAYIINHAEDKVIFVDA
CCCCCCCEEEEEECCEEEEEEEEEECCCCCEEEEEEEEECHHHHHHHHCCCCCCEEEEEH
TLLPLFSKLADNIPAVETIVLINAQPGIETPFPNVLHYEDLMAQVEAEFDWPVTDERQAM
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
GLCYTSGTTGNPKGVLYSHRSLYLHTMGENQATALAFTPDDIVMPVVPQFHAMAWGLPYS
EEEEECCCCCCCCCEEEECCEEEEEECCCCCEEEEEECCCCCEECCCCCHHHHHHCCCHH
AMFAGADLVMPGLHLNPVALADLIADEKITFPAGVPTIWTAMYQELRANPRDFSHVRCLA
HHHCCCHHCCCCCCCCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
VGGAAMPRGLIEAYERDFGVPVLHAWGMTELSPLGTISSLQPQHRQLSDHERWDLRAKQG
ECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHCCCCHHHHCCCCCHHCCCCCCC
YPIGGVELRIVNDAGEELPWDGTTVGELQARGPWVTAGYYKVEPTAEHFTADGWFRTGDV
CCCCCEEEEEECCCCCCCCCCCCEECCEECCCCEEECCEEEECCCHHHEECCCCEECCCE
ATINHEGYLGITDRTKDLVKSGGEWISSVELENTLMGHAKVTEAAVIAIPDERWSERPLA
EEECCCCEEEECCCHHHHHHCCCHHHHHEEHHHHHHCCCEECCEEEEEECCCCCCCCCEE
CVVLTKDAGEVEPSELLEYLEPLVAKFWLPERVVFVSEIPKTSVGKFDKKVLRARYANGE
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCC
LG
CC
>Mature Secondary Structure
MLRGLMMDYPLTVDRLLDHAYKLYPHKRVTTKQPDGSIHRYSFADLYHRVKRLGNVLHKL
CCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
GINQGDRVGTFAWNNYQHLELYYAIPCAGAVCHTLNIRLSTEQLAYIINHAEDKVIFVDA
CCCCCCCEEEEEECCEEEEEEEEEECCCCCEEEEEEEEECHHHHHHHHCCCCCCEEEEEH
TLLPLFSKLADNIPAVETIVLINAQPGIETPFPNVLHYEDLMAQVEAEFDWPVTDERQAM
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
GLCYTSGTTGNPKGVLYSHRSLYLHTMGENQATALAFTPDDIVMPVVPQFHAMAWGLPYS
EEEEECCCCCCCCCEEEECCEEEEEECCCCCEEEEEECCCCCEECCCCCHHHHHHCCCHH
AMFAGADLVMPGLHLNPVALADLIADEKITFPAGVPTIWTAMYQELRANPRDFSHVRCLA
HHHCCCHHCCCCCCCCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
VGGAAMPRGLIEAYERDFGVPVLHAWGMTELSPLGTISSLQPQHRQLSDHERWDLRAKQG
ECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHCCCCHHHHCCCCCHHCCCCCCC
YPIGGVELRIVNDAGEELPWDGTTVGELQARGPWVTAGYYKVEPTAEHFTADGWFRTGDV
CCCCCEEEEEECCCCCCCCCCCCEECCEECCCCEEECCEEEECCCHHHEECCCCEECCCE
ATINHEGYLGITDRTKDLVKSGGEWISSVELENTLMGHAKVTEAAVIAIPDERWSERPLA
EEECCCCEEEECCCHHHHHHCCCHHHHHEEHHHHHHCCCEECCEEEEEECCCCCCCCCEE
CVVLTKDAGEVEPSELLEYLEPLVAKFWLPERVVFVSEIPKTSVGKFDKKVLRARYANGE
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCC
LG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]