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The map label for this gene is puuB [H]

Identifier: 159899610

GI number: 159899610

Start: 3900461

End: 3901606

Strand: Reverse

Name: puuB [H]

Synonym: Haur_3091

Alternate gene names: 159899610

Gene position: 3901606-3900461 (Counterclockwise)

Preceding gene: 159899611

Following gene: 159899609

Centisome position: 61.48

GC content: 55.76

Gene sequence:

>1146_bases
ATGAGTGTTGGGCTGTGGCAAACCCCTGAGCCAACAGCCCAGCCCCAGCTTGTACAAACCTTGGTGATTGGTGCGGGCTT
GATTGGCTCGTACTTAGCGCTGCAAAGCCAAGCCGACGTTGTGCTCGATGCCCGTCACGTTGCTGGCGGCGCTTCGGGGC
GCAACGGTGGCTTGCTATTGACTGGCATTGCCAATTCGTATCAGGCGGCTTGCGCTGAATATGGCCGCGATTTAGCCCAA
AGCTTGTGGCGCACAACGATTCGTAATCGTGAGTTGATGATCGAATGGGCGACCAAGCTTGGCTCACCGGTACGGCGTTG
TGGTTCGTTAATTTTTGCCTATAGCGAGCAAGAACATGCCGAGCTAGCCCATGCGGCAGCCTTGATGCATGAAGATGGTT
TTAGCACCGAATGGCACGCCGAAGATCCAACCAAGCGTGGTTTTTGGGGCGCGTTGCACAACCCCGATGATGGTGCAATT
CAAGCCGCCTTGTTGACTGCGGCGCTCCTACGCGAAAGCGGCGCACAGCTCTATGAAGCTAGCGAGGTTTACGCGCTTGA
ATCAACTGCTGATGGCGTGTTGGTGCGGTCACGGACTGGCAATTGGCTAGCCCAACGGGTTTTGGTGGCAACTAACGCTT
GGACAGGCCTGCTGTTGCCTGAATTTAGTCAGTTGGTGATTCCGGCCCGTGGCCAGTTGTTGGCAACCGCTCCAATCGAG
CCAATTTTGAGCCAAGCCTGCTATTGCGATTATGGCTTTACCTATTTTCAGCAAACGCCTGCTGGCAATTTGGTGCTGGG
TGGTTTTCGTAATCGCGCGGTTGCTGAAGAGCAAACCTTTGCCGATTGCACAACACCGTTGATCCAAGATTTGCTTGATG
GCTTTTTGCAGCGCCACTTCCCTGAGGCTGCCGCTGCGCCGATTGAACGACGTTGGGCGGGCACAATGGGCTTTGCACCC
GATCATCGCCCCTTGGTTGGGCGTTTACGCCGCGATGACCGAATTGCCTTTGCTGTGGGGTTTAGCGGCCATGGCCTTGG
CTTAGGCTTGGTTGCCGCCGATGAACTGCTAGCCGAGTTGGCTGGCACACCTGCTGCCTTGTTTAGCGCTCGACGTTTTA
GCGAAACTGCCCACCTGTTCAGTTGA

Upstream 100 bases:

>100_bases
TCGAGCGAACTGCCCGCACTGCTGGCGTTGATTACTTCCTGACCAAGCCTTTCTCGCTTGATCGCCTGGAGCAAATTATC
AAGGAAGTGCTGGGCAAAGT

Downstream 100 bases:

>100_bases
TCAGGCTCAAACATCGGTCGCACGACCGTTGAGTGATTGTGCTCTGTGCATTACACTCTGCGTTCGTGCGATTGTTGTTT
TAGGAGGATCACCCTGATGA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSVGLWQTPEPTAQPQLVQTLVIGAGLIGSYLALQSQADVVLDARHVAGGASGRNGGLLLTGIANSYQAACAEYGRDLAQ
SLWRTTIRNRELMIEWATKLGSPVRRCGSLIFAYSEQEHAELAHAAALMHEDGFSTEWHAEDPTKRGFWGALHNPDDGAI
QAALLTAALLRESGAQLYEASEVYALESTADGVLVRSRTGNWLAQRVLVATNAWTGLLLPEFSQLVIPARGQLLATAPIE
PILSQACYCDYGFTYFQQTPAGNLVLGGFRNRAVAEEQTFADCTTPLIQDLLDGFLQRHFPEAAAAPIERRWAGTMGFAP
DHRPLVGRLRRDDRIAFAVGFSGHGLGLGLVAADELLAELAGTPAALFSARRFSETAHLFS

Sequences:

>Translated_381_residues
MSVGLWQTPEPTAQPQLVQTLVIGAGLIGSYLALQSQADVVLDARHVAGGASGRNGGLLLTGIANSYQAACAEYGRDLAQ
SLWRTTIRNRELMIEWATKLGSPVRRCGSLIFAYSEQEHAELAHAAALMHEDGFSTEWHAEDPTKRGFWGALHNPDDGAI
QAALLTAALLRESGAQLYEASEVYALESTADGVLVRSRTGNWLAQRVLVATNAWTGLLLPEFSQLVIPARGQLLATAPIE
PILSQACYCDYGFTYFQQTPAGNLVLGGFRNRAVAEEQTFADCTTPLIQDLLDGFLQRHFPEAAAAPIERRWAGTMGFAP
DHRPLVGRLRRDDRIAFAVGFSGHGLGLGLVAADELLAELAGTPAALFSARRFSETAHLFS
>Mature_380_residues
SVGLWQTPEPTAQPQLVQTLVIGAGLIGSYLALQSQADVVLDARHVAGGASGRNGGLLLTGIANSYQAACAEYGRDLAQS
LWRTTIRNRELMIEWATKLGSPVRRCGSLIFAYSEQEHAELAHAAALMHEDGFSTEWHAEDPTKRGFWGALHNPDDGAIQ
AALLTAALLRESGAQLYEASEVYALESTADGVLVRSRTGNWLAQRVLVATNAWTGLLLPEFSQLVIPARGQLLATAPIEP
ILSQACYCDYGFTYFQQTPAGNLVLGGFRNRAVAEEQTFADCTTPLIQDLLDGFLQRHFPEAAAAPIERRWAGTMGFAPD
HRPLVGRLRRDDRIAFAVGFSGHGLGLGLVAADELLAELAGTPAALFSARRFSETAHLFS

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=380, Percent_Identity=27.8947368421053, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41084; Mature: 40953

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVGLWQTPEPTAQPQLVQTLVIGAGLIGSYLALQSQADVVLDARHVAGGASGRNGGLLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCEEE
TGIANSYQAACAEYGRDLAQSLWRTTIRNRELMIEWATKLGSPVRRCGSLIFAYSEQEHA
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHCHHHHHHHHEEEEECCHHHH
ELAHAAALMHEDGFSTEWHAEDPTKRGFWGALHNPDDGAIQAALLTAALLRESGAQLYEA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEH
SEVYALESTADGVLVRSRTGNWLAQRVLVATNAWTGLLLPEFSQLVIPARGQLLATAPIE
HHEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCEEEECCHH
PILSQACYCDYGFTYFQQTPAGNLVLGGFRNRAVAEEQTFADCTTPLIQDLLDGFLQRHF
HHHHHHHHCCCCCHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
PEAAAAPIERRWAGTMGFAPDHRPLVGRLRRDDRIAFAVGFSGHGLGLGLVAADELLAEL
CHHHCCCHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHH
AGTPAALFSARRFSETAHLFS
CCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVGLWQTPEPTAQPQLVQTLVIGAGLIGSYLALQSQADVVLDARHVAGGASGRNGGLLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCCCCCEEE
TGIANSYQAACAEYGRDLAQSLWRTTIRNRELMIEWATKLGSPVRRCGSLIFAYSEQEHA
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHCHHHHHHHHEEEEECCHHHH
ELAHAAALMHEDGFSTEWHAEDPTKRGFWGALHNPDDGAIQAALLTAALLRESGAQLYEA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHEEH
SEVYALESTADGVLVRSRTGNWLAQRVLVATNAWTGLLLPEFSQLVIPARGQLLATAPIE
HHEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCEEEECCHH
PILSQACYCDYGFTYFQQTPAGNLVLGGFRNRAVAEEQTFADCTTPLIQDLLDGFLQRHF
HHHHHHHHCCCCCHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
PEAAAAPIERRWAGTMGFAPDHRPLVGRLRRDDRIAFAVGFSGHGLGLGLVAADELLAEL
CHHHCCCHHHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHH
AGTPAALFSARRFSETAHLFS
CCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]