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The map label for this gene is braE [H]

Identifier: 159899523

GI number: 159899523

Start: 3792895

End: 3794646

Strand: Reverse

Name: braE [H]

Synonym: Haur_3004

Alternate gene names: 159899523

Gene position: 3794646-3792895 (Counterclockwise)

Preceding gene: 159899524

Following gene: 159899522

Centisome position: 59.79

GC content: 52.0

Gene sequence:

>1752_bases
ATGCCAAATACAAAGCGCTATTTGATAACTGGTGGAATTGGCTTTGCAGTTGGGACATTCTTTTTATGGAATTACCCGAA
TCTTGCGCTTGGCTCGGCAATAATTGGTGGTCTGGTCATTGCCGCGCTCTGTTTGCTCTATAGTGCGCCCTTACCCGATA
TGGTTAAAGGTGGCTTAATTGCTGGTTTGTTCGGCGCAGTCATGACAATTGTCTATGGCCCCGATAAGCCAGGGGTGGCG
ATTATTGCGGGCATGCTCAGTGGTTTGGGCGCTGGTTTGCTGGCATTTCAACGCAAACCACAAGCAACGCCCCAAGCTGG
GGCTGCTTTGGGCGCTGGGGCTGGCGCAATTGCTGGCGCTTGGACGGCCCTCGCCCTCTTTTTAACTGCCTTTGTGATCG
CTCCCGCCGCTGGGGTTGATGCTAGCGAGTTTGCCGAGCATTTATCCGAAACCGTGATTTTGGCTGATGCGATGGGCTTA
GCCAGCGATATTCTGATTTTGCTGTTTGTGTTGGTGTTTTGTACCATCACTGGCGCATTATTGGGGGCGGCAGCGGGTGC
AGCACGGGCTTTGCCGTTGCGGCCTAATATCCCACTGTTAGCGGTGATTGCCCTGCCAGTGATCTTGATTCCCTTGATTG
ATCGTGCTGGCAACTTGCTCTTGCTTGATGCCTTGATTCCGATTTATATCTTTATTCTCCTGGCCTTAGGCTTGAACATT
GTGGTGGGTTATGCGGGCTTGCTTGATTTAGGTTATGCAGCCTTCTTTGCAATTGGTGCTTACACCACCGCCATGCTCTC
ATCGCCGCACCTTGGCATCAACATTAGTTTTTGGTTGGTAATTTGGGTGGCGGCGGCGGTCGCGGCAATTGCTGGTTTAG
CCTTGGGTGCGCCCACGCTACCCTTACGAGGCGACTATTTGGCGATTGTGACCCTTGGTTTCGGCGAAATTGTGCCGATT
CTCTTCCGCAACCTTGGTGGTGGCCCACCCACAGGAACCTTGACCTTGCGCTTCTTCGGCCTGCCAATTGGGATTGAACA
GATGGACTTGACTGGTGGTAATAAGGGGATTAACCCAATCTCGCCGCCAAATTTGCCGTTGATTGGCAATTTCGAGCCAT
CGAACAAAATTCCTTGGTATTACCTGTTGATTATCATCATGGGCTTGGCGATTTTCTTCATCAACCGCCTGCGTGATTCA
CGCCAAGGCCGCGCTTGGATGGCCATGCGCGAGGATGAATTGGCCGCTGATGCCATGGGTATCAACGTAGTACGCACCAA
GTTGTTAGCCTTCTCGATGGGGGCAATGTTCTCAGGCTTTGGTGGTGCGTTCTATGGCGCATTCATCGGAGCGATTTTCC
CAAGCTCATTCGACTTTAGCGTTTCAATCATCTTGCTCTGTATGGTGATTCTTGGTGGTTTGGGCAATATGGCCGGGGTG
ATTGTCGGTGGCTTGCTAATTCAGGGCGCTGATAAGATGTTTATCCCCAAAGGTGCCGAATTGTTCCGCGATATTGCTGA
CCAGAGAGCCCTCACCACCGGTGGTAGTACGGTCGGGGTTTCCGCTCTACAAGATTTGACCCAACAACGTTTATTGTTGT
TTGGCATCGTGTTGGTGGTGATGATGCTGATTCGACCAGAAGGCCTGTTGCCCAACGAGCGCCGTAAAGCCGAATTACAT
TCCGATGATGATCCGATCAATATCAATAGCGTGGCAGCCGAGGCGGATGCTGATCCGTCGTTGGCGGCGTAG

Upstream 100 bases:

>100_bases
AATGCGGTGATTTTCGCCATTCTTGTCTTGATTTTGCTCTTCCGACCAACTGGTTTGCTCGGACAAGAAGGTGGCCAAAA
AGCCTAGAAACGAGGATTGT

Downstream 100 bases:

>100_bases
GAGACCTGCATGGCACCTTTGCTGCTAGCTCGCGGCATTACCAAGCGTTTTGGTGGCCTAACCGCCGTGAGCGATGTTTC
ATTTGAGATTGAAAAAGGTT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MPNTKRYLITGGIGFAVGTFFLWNYPNLALGSAIIGGLVIAALCLLYSAPLPDMVKGGLIAGLFGAVMTIVYGPDKPGVA
IIAGMLSGLGAGLLAFQRKPQATPQAGAALGAGAGAIAGAWTALALFLTAFVIAPAAGVDASEFAEHLSETVILADAMGL
ASDILILLFVLVFCTITGALLGAAAGAARALPLRPNIPLLAVIALPVILIPLIDRAGNLLLLDALIPIYIFILLALGLNI
VVGYAGLLDLGYAAFFAIGAYTTAMLSSPHLGINISFWLVIWVAAAVAAIAGLALGAPTLPLRGDYLAIVTLGFGEIVPI
LFRNLGGGPPTGTLTLRFFGLPIGIEQMDLTGGNKGINPISPPNLPLIGNFEPSNKIPWYYLLIIIMGLAIFFINRLRDS
RQGRAWMAMREDELAADAMGINVVRTKLLAFSMGAMFSGFGGAFYGAFIGAIFPSSFDFSVSIILLCMVILGGLGNMAGV
IVGGLLIQGADKMFIPKGAELFRDIADQRALTTGGSTVGVSALQDLTQQRLLLFGIVLVVMMLIRPEGLLPNERRKAELH
SDDDPININSVAAEADADPSLAA

Sequences:

>Translated_583_residues
MPNTKRYLITGGIGFAVGTFFLWNYPNLALGSAIIGGLVIAALCLLYSAPLPDMVKGGLIAGLFGAVMTIVYGPDKPGVA
IIAGMLSGLGAGLLAFQRKPQATPQAGAALGAGAGAIAGAWTALALFLTAFVIAPAAGVDASEFAEHLSETVILADAMGL
ASDILILLFVLVFCTITGALLGAAAGAARALPLRPNIPLLAVIALPVILIPLIDRAGNLLLLDALIPIYIFILLALGLNI
VVGYAGLLDLGYAAFFAIGAYTTAMLSSPHLGINISFWLVIWVAAAVAAIAGLALGAPTLPLRGDYLAIVTLGFGEIVPI
LFRNLGGGPPTGTLTLRFFGLPIGIEQMDLTGGNKGINPISPPNLPLIGNFEPSNKIPWYYLLIIIMGLAIFFINRLRDS
RQGRAWMAMREDELAADAMGINVVRTKLLAFSMGAMFSGFGGAFYGAFIGAIFPSSFDFSVSIILLCMVILGGLGNMAGV
IVGGLLIQGADKMFIPKGAELFRDIADQRALTTGGSTVGVSALQDLTQQRLLLFGIVLVVMMLIRPEGLLPNERRKAELH
SDDDPININSVAAEADADPSLAA
>Mature_582_residues
PNTKRYLITGGIGFAVGTFFLWNYPNLALGSAIIGGLVIAALCLLYSAPLPDMVKGGLIAGLFGAVMTIVYGPDKPGVAI
IAGMLSGLGAGLLAFQRKPQATPQAGAALGAGAGAIAGAWTALALFLTAFVIAPAAGVDASEFAEHLSETVILADAMGLA
SDILILLFVLVFCTITGALLGAAAGAARALPLRPNIPLLAVIALPVILIPLIDRAGNLLLLDALIPIYIFILLALGLNIV
VGYAGLLDLGYAAFFAIGAYTTAMLSSPHLGINISFWLVIWVAAAVAAIAGLALGAPTLPLRGDYLAIVTLGFGEIVPIL
FRNLGGGPPTGTLTLRFFGLPIGIEQMDLTGGNKGINPISPPNLPLIGNFEPSNKIPWYYLLIIIMGLAIFFINRLRDSR
QGRAWMAMREDELAADAMGINVVRTKLLAFSMGAMFSGFGGAFYGAFIGAIFPSSFDFSVSIILLCMVILGGLGNMAGVI
VGGLLIQGADKMFIPKGAELFRDIADQRALTTGGSTVGVSALQDLTQQRLLLFGIVLVVMMLIRPEGLLPNERRKAELHS
DDDPININSVAAEADADPSLAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=352, Percent_Identity=36.0795454545455, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 60567; Mature: 60436

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00066 HMG_COA_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNTKRYLITGGIGFAVGTFFLWNYPNLALGSAIIGGLVIAALCLLYSAPLPDMVKGGLI
CCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AGLFGAVMTIVYGPDKPGVAIIAGMLSGLGAGLLAFQRKPQATPQAGAALGAGAGAIAGA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHHH
WTALALFLTAFVIAPAAGVDASEFAEHLSETVILADAMGLASDILILLFVLVFCTITGAL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAAAGAARALPLRPNIPLLAVIALPVILIPLIDRAGNLLLLDALIPIYIFILLALGLNI
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
VVGYAGLLDLGYAAFFAIGAYTTAMLSSPHLGINISFWLVIWVAAAVAAIAGLALGAPTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCC
PLRGDYLAIVTLGFGEIVPILFRNLGGGPPTGTLTLRFFGLPIGIEQMDLTGGNKGINPI
CCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCC
SPPNLPLIGNFEPSNKIPWYYLLIIIMGLAIFFINRLRDSRQGRAWMAMREDELAADAMG
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
INVVRTKLLAFSMGAMFSGFGGAFYGAFIGAIFPSSFDFSVSIILLCMVILGGLGNMAGV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
IVGGLLIQGADKMFIPKGAELFRDIADQRALTTGGSTVGVSALQDLTQQRLLLFGIVLVV
HHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MMLIRPEGLLPNERRKAELHSDDDPININSVAAEADADPSLAA
HHHHCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PNTKRYLITGGIGFAVGTFFLWNYPNLALGSAIIGGLVIAALCLLYSAPLPDMVKGGLI
CCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AGLFGAVMTIVYGPDKPGVAIIAGMLSGLGAGLLAFQRKPQATPQAGAALGAGAGAIAGA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHHH
WTALALFLTAFVIAPAAGVDASEFAEHLSETVILADAMGLASDILILLFVLVFCTITGAL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAAAGAARALPLRPNIPLLAVIALPVILIPLIDRAGNLLLLDALIPIYIFILLALGLNI
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
VVGYAGLLDLGYAAFFAIGAYTTAMLSSPHLGINISFWLVIWVAAAVAAIAGLALGAPTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCC
PLRGDYLAIVTLGFGEIVPILFRNLGGGPPTGTLTLRFFGLPIGIEQMDLTGGNKGINPI
CCCCCEEEEEEECHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCC
SPPNLPLIGNFEPSNKIPWYYLLIIIMGLAIFFINRLRDSRQGRAWMAMREDELAADAMG
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
INVVRTKLLAFSMGAMFSGFGGAFYGAFIGAIFPSSFDFSVSIILLCMVILGGLGNMAGV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
IVGGLLIQGADKMFIPKGAELFRDIADQRALTTGGSTVGVSALQDLTQQRLLLFGIVLVV
HHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MMLIRPEGLLPNERRKAELHSDDDPININSVAAEADADPSLAA
HHHHCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]