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The map label for this gene is malS [C]

Identifier: 159899488

GI number: 159899488

Start: 3745721

End: 3747295

Strand: Reverse

Name: malS [C]

Synonym: Haur_2969

Alternate gene names: 159899488

Gene position: 3747295-3745721 (Counterclockwise)

Preceding gene: 159899489

Following gene: 159899485

Centisome position: 59.04

GC content: 50.16

Gene sequence:

>1575_bases
ATGAAAAAGCTTTTGAGTGGCCTGCTTTTAGCCGGGGTGATTGTGGGCTGTGGCGGCCAAGCAACTCCAACCGCTGTTCC
GGCAACAGTGACCAGCCAATCAACCCCAACCAGCCAAGCCTTGAATCCAACCGCTACTGCCGCTACTGTGGTTGATAACT
CAATTACGCCAACGCCATTGCCAACCAAACCAACAGCCCCAATCTTTACCGCTGACGATGAGCGTTGGGCTGGCCGTTCG
ATCTACTTTATTATGATCGATCGCTTTGCCAATGGCGACCCAAGCAACGACAACGCCGATGGCTTTGGGGCAGATCGTAG
CGATCCACGGCGTTGGCATGGCGGCGATTTTCGCGGCATTATCGAGCGGCTCGATTACATCAAAGGCATGGGCTTTGGCG
GCATTTGGATCACGCCAGTCAGTAAGCAAAATTCAACCAATGCCTACCATGGCTACTGGCAATACGACCCCTACCAAATT
GACCCGCATTTTGGCACGCTGGAAGAATTGCGCGAATTGGTCAGCGAAGCCCACAAACGCGATATATTGGTGATGCTCGA
TGTTGTGCCCAATCATATGGGCGATTTCTTGCCTGGCTCGAAAGCTGCCCCGCCATTCGATGACCCAACCTGGTATCACA
ACAAGGGCAACATTCAAAATTATGGCAATCAACAAGAGGTTGAAGATGGCGATTTGCTCGGGCTTGATGATTTAGATCAG
GATAATCCTGCTACCCGTGCTGAATTACTCAAATGGATTGCTTGGCTTAAAACCGAAACTGGGCTTGATGGCTTGCGAGT
TGATACGGCCAAACATTTGCCCAAAGATTTTCTCCGTGAGTTTGATCAAGCGGCCAATACGTTTTCGCTGGCTGAGGTAT
TTAGCAGCGATGCGGGCTATGTTGCGCCCTACACCGAATTTAACGACGCAATTTTGGATTACCCCTTGCACAGCGCCTTT
AAAGAAAGTTTAGTCGGTGGTCGCACGTTGTTGGTGATTCAGCGCGTGCTCGAAAATGCCGATCAACAGTATCGCAATGT
CCATGTCAACGGCACATTTCTCGATAATCACGATAACGAGCGCTTTTTATGCTTGGCAACTGGCGGCCCCAACGCCGATA
AAACCACTCAATTGCGGCAAGCTTTGGCGGTGCTCTATAGTTTGCGTGGCATTCCGATTGTCTATTATGGCACCGAGCAA
GAACTCAACGGCTGCAAAGATCCCTTCAACCGCGAAGATGCCTTTGAATTGAATGCGACTGATGTACCAGTCTATCAATG
GATCAGCCAACTCAACCAGATTCGCCAAGCCCATCCAGCCTTGCAACGTGGCACACTCGAAAGCCGCACAACTCCTAGCG
ATGCATGGGCCTTTCAACGCACGGCGGGCAACGATACGGTCGTAGTTTGCATCAATAACACATGGAAATCGCTCGACTTG
GCAGTAACTGGTTTGACTGAAATTGCTGATGGTGAGGTGTTGACTGATGCGCTTGGTAGCGGTCAAATGAGCGTTAAAAA
TGGCGAAATGAATTGTGCTCTACAACCAAAACAGGTGCTGATCTATACCCGTTAA

Upstream 100 bases:

>100_bases
GCCGCTGCGTTAAAATCCGATCCCCAAACCCCATCAACCGATCCCCGATTCTCAAATACCGCTACAATAACCCAAGCACT
CTGCTAGTGAAGGACTAAAT

Downstream 100 bases:

>100_bases
TTCTTGTGGTAAACCCAGCGCCAAGCCTGAAGCTGGTTTGGCGCTGGAGTTTGTTGGCTACAAAGCCTCTATTTCGCCGC
GCAAACGACCAAGCGCTTGC

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: Beta-amylase; Alpha-amylase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKKLLSGLLLAGVIVGCGGQATPTAVPATVTSQSTPTSQALNPTATAATVVDNSITPTPLPTKPTAPIFTADDERWAGRS
IYFIMIDRFANGDPSNDNADGFGADRSDPRRWHGGDFRGIIERLDYIKGMGFGGIWITPVSKQNSTNAYHGYWQYDPYQI
DPHFGTLEELRELVSEAHKRDILVMLDVVPNHMGDFLPGSKAAPPFDDPTWYHNKGNIQNYGNQQEVEDGDLLGLDDLDQ
DNPATRAELLKWIAWLKTETGLDGLRVDTAKHLPKDFLREFDQAANTFSLAEVFSSDAGYVAPYTEFNDAILDYPLHSAF
KESLVGGRTLLVIQRVLENADQQYRNVHVNGTFLDNHDNERFLCLATGGPNADKTTQLRQALAVLYSLRGIPIVYYGTEQ
ELNGCKDPFNREDAFELNATDVPVYQWISQLNQIRQAHPALQRGTLESRTTPSDAWAFQRTAGNDTVVVCINNTWKSLDL
AVTGLTEIADGEVLTDALGSGQMSVKNGEMNCALQPKQVLIYTR

Sequences:

>Translated_524_residues
MKKLLSGLLLAGVIVGCGGQATPTAVPATVTSQSTPTSQALNPTATAATVVDNSITPTPLPTKPTAPIFTADDERWAGRS
IYFIMIDRFANGDPSNDNADGFGADRSDPRRWHGGDFRGIIERLDYIKGMGFGGIWITPVSKQNSTNAYHGYWQYDPYQI
DPHFGTLEELRELVSEAHKRDILVMLDVVPNHMGDFLPGSKAAPPFDDPTWYHNKGNIQNYGNQQEVEDGDLLGLDDLDQ
DNPATRAELLKWIAWLKTETGLDGLRVDTAKHLPKDFLREFDQAANTFSLAEVFSSDAGYVAPYTEFNDAILDYPLHSAF
KESLVGGRTLLVIQRVLENADQQYRNVHVNGTFLDNHDNERFLCLATGGPNADKTTQLRQALAVLYSLRGIPIVYYGTEQ
ELNGCKDPFNREDAFELNATDVPVYQWISQLNQIRQAHPALQRGTLESRTTPSDAWAFQRTAGNDTVVVCINNTWKSLDL
AVTGLTEIADGEVLTDALGSGQMSVKNGEMNCALQPKQVLIYTR
>Mature_524_residues
MKKLLSGLLLAGVIVGCGGQATPTAVPATVTSQSTPTSQALNPTATAATVVDNSITPTPLPTKPTAPIFTADDERWAGRS
IYFIMIDRFANGDPSNDNADGFGADRSDPRRWHGGDFRGIIERLDYIKGMGFGGIWITPVSKQNSTNAYHGYWQYDPYQI
DPHFGTLEELRELVSEAHKRDILVMLDVVPNHMGDFLPGSKAAPPFDDPTWYHNKGNIQNYGNQQEVEDGDLLGLDDLDQ
DNPATRAELLKWIAWLKTETGLDGLRVDTAKHLPKDFLREFDQAANTFSLAEVFSSDAGYVAPYTEFNDAILDYPLHSAF
KESLVGGRTLLVIQRVLENADQQYRNVHVNGTFLDNHDNERFLCLATGGPNADKTTQLRQALAVLYSLRGIPIVYYGTEQ
ELNGCKDPFNREDAFELNATDVPVYQWISQLNQIRQAHPALQRGTLESRTTPSDAWAFQRTAGNDTVVVCINNTWKSLDL
AVTGLTEIADGEVLTDALGSGQMSVKNGEMNCALQPKQVLIYTR

Specific function: The precursor protein is proteolytically cleaved to produce multiform beta-amylases and a 48 kDa alpha-amylase after secretion [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI4502085, Length=434, Percent_Identity=23.2718894009217, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI10280622, Length=434, Percent_Identity=23.0414746543779, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789995, Length=132, Percent_Identity=37.8787878787879, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790687, Length=349, Percent_Identity=24.9283667621776, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586587, Length=237, Percent_Identity=28.2700421940928, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221330053, Length=104, Percent_Identity=38.4615384615385, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549022, Length=202, Percent_Identity=27.7227722772277, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17137384, Length=265, Percent_Identity=26.7924528301887, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586599, Length=104, Percent_Identity=33.6538461538462, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586591, Length=190, Percent_Identity=29.4736842105263, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR005085
- InterPro:   IPR008985
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR001554
- InterPro:   IPR018238
- InterPro:   IPR000125
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF03423 CBM_25; PF01373 Glyco_hydro_14 [H]

EC number: =3.2.1.2; =3.2.1.1 [H]

Molecular weight: Translated: 57858; Mature: 57858

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLSGLLLAGVIVGCGGQATPTAVPATVTSQSTPTSQALNPTATAATVVDNSITPTPL
CHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHCCCCCHHHHHEECCCCCCCCC
PTKPTAPIFTADDERWAGRSIYFIMIDRFANGDPSNDNADGFGADRSDPRRWHGGDFRGI
CCCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
IERLDYIKGMGFGGIWITPVSKQNSTNAYHGYWQYDPYQIDPHFGTLEELRELVSEAHKR
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCEEECCCCCCHHHHHHHHHHHHHC
DILVMLDVVPNHMGDFLPGSKAAPPFDDPTWYHNKGNIQNYGNQQEVEDGDLLGLDDLDQ
CEEEEEEHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEECCCCCCC
DNPATRAELLKWIAWLKTETGLDGLRVDTAKHLPKDFLREFDQAANTFSLAEVFSSDAGY
CCCHHHHHHHHHHHHHHHCCCCCCEEECHHHHCHHHHHHHHHHHCCHHHHHHHHCCCCCC
VAPYTEFNDAILDYPLHSAFKESLVGGRTLLVIQRVLENADQQYRNVHVNGTFLDNHDNE
CCCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCC
RFLCLATGGPNADKTTQLRQALAVLYSLRGIPIVYYGTEQELNGCKDPFNREDAFELNAT
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCEEECCC
DVPVYQWISQLNQIRQAHPALQRGTLESRTTPSDAWAFQRTAGNDTVVVCINNTWKSLDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCCCCEEEEEECCCCCEEEE
AVTGLTEIADGEVLTDALGSGQMSVKNGEMNCALQPKQVLIYTR
EEECHHHHCCCCHHHHHCCCCCEEEECCCEEEEECCCEEEEEEC
>Mature Secondary Structure
MKKLLSGLLLAGVIVGCGGQATPTAVPATVTSQSTPTSQALNPTATAATVVDNSITPTPL
CHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHCCCCCHHHHHEECCCCCCCCC
PTKPTAPIFTADDERWAGRSIYFIMIDRFANGDPSNDNADGFGADRSDPRRWHGGDFRGI
CCCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
IERLDYIKGMGFGGIWITPVSKQNSTNAYHGYWQYDPYQIDPHFGTLEELRELVSEAHKR
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCEEECCCCCCHHHHHHHHHHHHHC
DILVMLDVVPNHMGDFLPGSKAAPPFDDPTWYHNKGNIQNYGNQQEVEDGDLLGLDDLDQ
CEEEEEEHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEECCCCCCC
DNPATRAELLKWIAWLKTETGLDGLRVDTAKHLPKDFLREFDQAANTFSLAEVFSSDAGY
CCCHHHHHHHHHHHHHHHCCCCCCEEECHHHHCHHHHHHHHHHHCCHHHHHHHHCCCCCC
VAPYTEFNDAILDYPLHSAFKESLVGGRTLLVIQRVLENADQQYRNVHVNGTFLDNHDNE
CCCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCC
RFLCLATGGPNADKTTQLRQALAVLYSLRGIPIVYYGTEQELNGCKDPFNREDAFELNAT
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCCCEEECCC
DVPVYQWISQLNQIRQAHPALQRGTLESRTTPSDAWAFQRTAGNDTVVVCINNTWKSLDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCCCCEEEEEECCCCCEEEE
AVTGLTEIADGEVLTDALGSGQMSVKNGEMNCALQPKQVLIYTR
EEECHHHHCCCCHHHHHCCCCCEEEECCCEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2435707; 2464578; 2438660; 1827035 [H]