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The map label for this gene is estA [H]

Identifier: 159899437

GI number: 159899437

Start: 3692240

End: 3692896

Strand: Reverse

Name: estA [H]

Synonym: Haur_2918

Alternate gene names: 159899437

Gene position: 3692896-3692240 (Counterclockwise)

Preceding gene: 159899438

Following gene: 159899435

Centisome position: 58.19

GC content: 49.77

Gene sequence:

>657_bases
ATGAGTCAAATTTCATTAACCCACGAAGTCGTTAAACCACGCCAAACCAGTGCCGAGGCTAAAGCGCCCTTGTTGGTCAT
GTTGCATGGCGTGGGAGCCAACGAGCATGATCTGTTGCCTTTGGCTCAATATCTCGACCCACGTTTGTGTGTCGTTTCGG
CTCGCGCACCCCATCGCTATCAATTTGGCGGCTTTTCGTGGTTCGATATTCATTGGCATGCCAAGGGTTTTAATATTGAT
ACCAATCAAGCCCAACAAAGCTGGGAGACGGTGCAGCGCTTTTTGGGTGAAGCATGCAGCGCCTACGATTGCGATCCTAA
ACAAATTTATTTGGGCGGTTTTTCGCAAGGCGCAATTATGAGTTTAGGTGCAACCCTGACCAAGCCCGAATTGATTGCTG
GCACAATTTTGATGAGTGGCCGTTGGATGCCCGAAGTTGGCCCGCAAACCGACCGCGAACACATCGCCAACAAGCCAATT
GTGGCGGTTCATGGCGTATACGATGAGGTTATTCCAATTCAATATGGGCGGGCGATTCGCGATTTTCTGCAAACCCTGCC
TGTGCAACTGGAATACCATGAATTTGCCATGGGCCACGAAATTAATTTGGATAGTTTGCAAGTGGTGGTCAAGTGGCTCA
AGCAGCAGCTTGGCTGA

Upstream 100 bases:

>100_bases
GCCAAAGCTGTGCGTTTTATCGTCGAATCGCCCTATTTTACCGGCGAAACCATCTTTGTTGATGGCGGTCGTCGCTGGGC
CTAAGGAGCATCCTACATCC

Downstream 100 bases:

>100_bases
TGCCCTCACCCCCTATCCTCCTCCTTACAAGAGTAGGTTTTAAGCTCCCTCACCCCCAACCCCTCTCCCGCCGAGCGGGA
GAGGGGGAAACTTGATCAAA

Product: phospholipase/carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSQISLTHEVVKPRQTSAEAKAPLLVMLHGVGANEHDLLPLAQYLDPRLCVVSARAPHRYQFGGFSWFDIHWHAKGFNID
TNQAQQSWETVQRFLGEACSAYDCDPKQIYLGGFSQGAIMSLGATLTKPELIAGTILMSGRWMPEVGPQTDREHIANKPI
VAVHGVYDEVIPIQYGRAIRDFLQTLPVQLEYHEFAMGHEINLDSLQVVVKWLKQQLG

Sequences:

>Translated_218_residues
MSQISLTHEVVKPRQTSAEAKAPLLVMLHGVGANEHDLLPLAQYLDPRLCVVSARAPHRYQFGGFSWFDIHWHAKGFNID
TNQAQQSWETVQRFLGEACSAYDCDPKQIYLGGFSQGAIMSLGATLTKPELIAGTILMSGRWMPEVGPQTDREHIANKPI
VAVHGVYDEVIPIQYGRAIRDFLQTLPVQLEYHEFAMGHEINLDSLQVVVKWLKQQLG
>Mature_217_residues
SQISLTHEVVKPRQTSAEAKAPLLVMLHGVGANEHDLLPLAQYLDPRLCVVSARAPHRYQFGGFSWFDIHWHAKGFNIDT
NQAQQSWETVQRFLGEACSAYDCDPKQIYLGGFSQGAIMSLGATLTKPELIAGTILMSGRWMPEVGPQTDREHIANKPIV
AVHGVYDEVIPIQYGRAIRDFLQTLPVQLEYHEFAMGHEINLDSLQVVVKWLKQQLG

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323147, Length=235, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24446; Mature: 24315

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQISLTHEVVKPRQTSAEAKAPLLVMLHGVGANEHDLLPLAQYLDPRLCVVSARAPHRY
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCE
QFGGFSWFDIHWHAKGFNIDTNQAQQSWETVQRFLGEACSAYDCDPKQIYLGGFSQGAIM
ECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
SLGATLTKPELIAGTILMSGRWMPEVGPQTDREHIANKPIVAVHGVYDEVIPIQYGRAIR
HHCCCCCCCHHHHEEEEECCCCCCCCCCCCCHHHHCCCCEEEEECCCHHHCCHHHHHHHH
DFLQTLPVQLEYHEFAMGHEINLDSLQVVVKWLKQQLG
HHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQISLTHEVVKPRQTSAEAKAPLLVMLHGVGANEHDLLPLAQYLDPRLCVVSARAPHRY
CCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCE
QFGGFSWFDIHWHAKGFNIDTNQAQQSWETVQRFLGEACSAYDCDPKQIYLGGFSQGAIM
ECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
SLGATLTKPELIAGTILMSGRWMPEVGPQTDREHIANKPIVAVHGVYDEVIPIQYGRAIR
HHCCCCCCCHHHHEEEEECCCCCCCCCCCCCHHHHCCCCEEEEECCCHHHCCHHHHHHHH
DFLQTLPVQLEYHEFAMGHEINLDSLQVVVKWLKQQLG
HHHHHCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA