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The map label for this gene is yhcA [H]

Identifier: 159899280

GI number: 159899280

Start: 3515294

End: 3516931

Strand: Reverse

Name: yhcA [H]

Synonym: Haur_2761

Alternate gene names: 159899280

Gene position: 3516931-3515294 (Counterclockwise)

Preceding gene: 159899285

Following gene: 159899279

Centisome position: 55.41

GC content: 51.95

Gene sequence:

>1638_bases
ATGGCGGCGACAGCGCAAGATTCAGAGCAATCACCAAAAGGCGGTTTAGCCTACAAATGGAAGGTATTAATTTCGGTTAT
ATTCGGGATCTTCATGGTGATTCTGGATACGACTGTCGTAAACGTGGCATTTCGCACGGTGCAACAAGATCTGGGGGCGA
CGATCAACGAAGCCCAATGGATTTTGAGCATTTATGTCTTGATGTTGGGGATTAGCACGCCAATTTCGGGCTATATGGCT
GATCGCTTTGGCCTCAAGCGCACTTATGTGATCGGTCTTTCAATCTTTGTACTTGGCTCGTTTTTGTGTGGGGTCGCACC
AACAATTGAAACGTTGGTCGTGGCGCGGGCAATTCAGGGGCTTGGTGGTGGTTTATCAACTCCACTTGGCACGGCCTTCA
TCTATGGAGCTTTCCCTCCCAATGAGCGTGGCAAAGCCCTTGGGGTGTTTGGGATTGCCATGGTGCTTGGGCCTGCTTTA
GGGCCAATTCTCGGTGGCTTGTTGGTCGATAACGATCTTTGGCGCTGGATTTTCTTTATCAATGTGCCAATTGGCGTGCT
GGGTGTAAGTTTGGCGATTCGCTGGCTCAAGCCAACCCCACCAACGGGCAAACCCAAACTTGATGTGCTAGGCTTGATCA
CCTCCACGGTTGGATTTGGCTCGGTGCTGTATGCCGCCACGATTGCCGCCGAACAGGGCTGGGAATCGGGCAGCGTCACG
ATGTGGTTTATCATCGGTGGCATTAGCTTGCTCCTCTTTGGTTTGATTGAATTATTCGTGGCCAAAGAACCCCTACTCGA
TTTGCGCTTATTCAAAAAACCAACCTTTACTATGGCCAATTTGGTGGGCTGGGTTAGCGTCTTGGCATTGTTCGGGGCCG
AATTTTTGTTGCCCTTGTATTTGCAGGTGCTACGTGGGCGCACCGCTTTAGAAACTGGCTTTATCTTGCTGCCCTTGGCG
ATTGCTGCTGGCTTTGCTACTCCAACGGCTGGGCGTTTATACGACAAAATCGGCCCGCGCCCGTTGATTGTGACTGGCTA
TGCGATTTTGATTCTCAACACCTGGCAACTCTCGAAATTGCAAGCCGATACCTCAATTGGCTGGATTATGTTCTTGCTGG
CCTTACGTGGCTTGGCGCTGGGCATGACCGTGCAAACCACCTTCGTCACAGCACTATCGGATGTTGATTTGCCCAAAGTG
CCACGTGGTTCGGCTTTGATCAATGCTACGCGCCAAGTGATTCAATCGATTGGCGTGGCCGTGTTGGCGACGATTCTCGC
CAGCACAACCTCAGTCGCAACCAAAGAATTTCAAGAGCAATCGCAGGCTCGTGCCGCCGAGAATGCCCAAGCTGGAGTTG
CAACTGCCGCATTTGGCCTGTGCGAAACCCCAGGCGTTGACCCATCCAAGAATTTGCCAGCAGGCATGGAGCAAGCACCA
GCTGTGATGCAAGATCAAGTTCGGAGCAGCATTCAATTGGCTTGTGCTGAACAAGTTGCTGGCTTTGAAAAAACCTATCA
CTTCACCTTCTATGTAGCAATGGTAGCACTCTTCCTTGGCTTATTCCTGCCTGGTTGGCCGCTCAAGTGGGCCGGTCGCG
GTGGAACTGCCGGAGCGGCGGCAACTGCTAGCCATTAG

Upstream 100 bases:

>100_bases
TTGCAATCTATCAGCAGTAGTTTGCATGGGCTGGAAGCAACCAGAAACTGCTGCTTTTTGATGTGGAATAAATTGGACAT
GCTAGTTGCGAGGGTATTTC

Downstream 100 bases:

>100_bases
GATGTAATGTAAATGCGTACCCTCACCCCCTAGCCTCCTAGTCCGCCGGGGAACCATCAGCGATTCTCCCTTCTCCCGCC
GCAGTGGGAGAGGGGCTGGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MAATAQDSEQSPKGGLAYKWKVLISVIFGIFMVILDTTVVNVAFRTVQQDLGATINEAQWILSIYVLMLGISTPISGYMA
DRFGLKRTYVIGLSIFVLGSFLCGVAPTIETLVVARAIQGLGGGLSTPLGTAFIYGAFPPNERGKALGVFGIAMVLGPAL
GPILGGLLVDNDLWRWIFFINVPIGVLGVSLAIRWLKPTPPTGKPKLDVLGLITSTVGFGSVLYAATIAAEQGWESGSVT
MWFIIGGISLLLFGLIELFVAKEPLLDLRLFKKPTFTMANLVGWVSVLALFGAEFLLPLYLQVLRGRTALETGFILLPLA
IAAGFATPTAGRLYDKIGPRPLIVTGYAILILNTWQLSKLQADTSIGWIMFLLALRGLALGMTVQTTFVTALSDVDLPKV
PRGSALINATRQVIQSIGVAVLATILASTTSVATKEFQEQSQARAAENAQAGVATAAFGLCETPGVDPSKNLPAGMEQAP
AVMQDQVRSSIQLACAEQVAGFEKTYHFTFYVAMVALFLGLFLPGWPLKWAGRGGTAGAAATASH

Sequences:

>Translated_545_residues
MAATAQDSEQSPKGGLAYKWKVLISVIFGIFMVILDTTVVNVAFRTVQQDLGATINEAQWILSIYVLMLGISTPISGYMA
DRFGLKRTYVIGLSIFVLGSFLCGVAPTIETLVVARAIQGLGGGLSTPLGTAFIYGAFPPNERGKALGVFGIAMVLGPAL
GPILGGLLVDNDLWRWIFFINVPIGVLGVSLAIRWLKPTPPTGKPKLDVLGLITSTVGFGSVLYAATIAAEQGWESGSVT
MWFIIGGISLLLFGLIELFVAKEPLLDLRLFKKPTFTMANLVGWVSVLALFGAEFLLPLYLQVLRGRTALETGFILLPLA
IAAGFATPTAGRLYDKIGPRPLIVTGYAILILNTWQLSKLQADTSIGWIMFLLALRGLALGMTVQTTFVTALSDVDLPKV
PRGSALINATRQVIQSIGVAVLATILASTTSVATKEFQEQSQARAAENAQAGVATAAFGLCETPGVDPSKNLPAGMEQAP
AVMQDQVRSSIQLACAEQVAGFEKTYHFTFYVAMVALFLGLFLPGWPLKWAGRGGTAGAAATASH
>Mature_544_residues
AATAQDSEQSPKGGLAYKWKVLISVIFGIFMVILDTTVVNVAFRTVQQDLGATINEAQWILSIYVLMLGISTPISGYMAD
RFGLKRTYVIGLSIFVLGSFLCGVAPTIETLVVARAIQGLGGGLSTPLGTAFIYGAFPPNERGKALGVFGIAMVLGPALG
PILGGLLVDNDLWRWIFFINVPIGVLGVSLAIRWLKPTPPTGKPKLDVLGLITSTVGFGSVLYAATIAAEQGWESGSVTM
WFIIGGISLLLFGLIELFVAKEPLLDLRLFKKPTFTMANLVGWVSVLALFGAEFLLPLYLQVLRGRTALETGFILLPLAI
AAGFATPTAGRLYDKIGPRPLIVTGYAILILNTWQLSKLQADTSIGWIMFLLALRGLALGMTVQTTFVTALSDVDLPKVP
RGSALINATRQVIQSIGVAVLATILASTTSVATKEFQEQSQARAAENAQAGVATAAFGLCETPGVDPSKNLPAGMEQAPA
VMQDQVRSSIQLACAEQVAGFEKTYHFTFYVAMVALFLGLFLPGWPLKWAGRGGTAGAAATASH

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=433, Percent_Identity=28.8683602771363, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1790195, Length=399, Percent_Identity=31.8295739348371, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI87081983, Length=423, Percent_Identity=28.3687943262411, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788710, Length=444, Percent_Identity=26.5765765765766, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=27.4939172749392, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI48994889, Length=200, Percent_Identity=31, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI2367379, Length=174, Percent_Identity=29.8850574712644, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=488, Percent_Identity=22.7459016393443, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323735, Length=319, Percent_Identity=26.9592476489028, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=437, Percent_Identity=24.0274599542334, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321663, Length=212, Percent_Identity=26.8867924528302, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=393, Percent_Identity=24.4274809160305, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=284, Percent_Identity=25, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57816; Mature: 57685

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATAQDSEQSPKGGLAYKWKVLISVIFGIFMVILDTTVVNVAFRTVQQDLGATINEAQW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ILSIYVLMLGISTPISGYMADRFGLKRTYVIGLSIFVLGSFLCGVAPTIETLVVARAIQG
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LGGGLSTPLGTAFIYGAFPPNERGKALGVFGIAMVLGPALGPILGGLLVDNDLWRWIFFI
CCCCCCCCHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHEE
NVPIGVLGVSLAIRWLKPTPPTGKPKLDVLGLITSTVGFGSVLYAATIAAEQGWESGSVT
ECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
MWFIIGGISLLLFGLIELFVAKEPLLDLRLFKKPTFTMANLVGWVSVLALFGAEFLLPLY
EHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQVLRGRTALETGFILLPLAIAAGFATPTAGRLYDKIGPRPLIVTGYAILILNTWQLSKL
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECEEEEEEECHHHHHH
QADTSIGWIMFLLALRGLALGMTVQTTFVTALSDVDLPKVPRGSALINATRQVIQSIGVA
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
VLATILASTTSVATKEFQEQSQARAAENAQAGVATAAFGLCETPGVDPSKNLPAGMEQAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHH
AVMQDQVRSSIQLACAEQVAGFEKTYHFTFYVAMVALFLGLFLPGWPLKWAGRGGTAGAA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCC
ATASH
CCCCC
>Mature Secondary Structure 
AATAQDSEQSPKGGLAYKWKVLISVIFGIFMVILDTTVVNVAFRTVQQDLGATINEAQW
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ILSIYVLMLGISTPISGYMADRFGLKRTYVIGLSIFVLGSFLCGVAPTIETLVVARAIQG
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LGGGLSTPLGTAFIYGAFPPNERGKALGVFGIAMVLGPALGPILGGLLVDNDLWRWIFFI
CCCCCCCCHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHEE
NVPIGVLGVSLAIRWLKPTPPTGKPKLDVLGLITSTVGFGSVLYAATIAAEQGWESGSVT
ECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
MWFIIGGISLLLFGLIELFVAKEPLLDLRLFKKPTFTMANLVGWVSVLALFGAEFLLPLY
EHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQVLRGRTALETGFILLPLAIAAGFATPTAGRLYDKIGPRPLIVTGYAILILNTWQLSKL
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECEEEEEEECHHHHHH
QADTSIGWIMFLLALRGLALGMTVQTTFVTALSDVDLPKVPRGSALINATRQVIQSIGVA
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
VLATILASTTSVATKEFQEQSQARAAENAQAGVATAAFGLCETPGVDPSKNLPAGMEQAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHH
AVMQDQVRSSIQLACAEQVAGFEKTYHFTFYVAMVALFLGLFLPGWPLKWAGRGGTAGAA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCC
ATASH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]