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The map label for this gene is glnH [H]

Identifier: 159899279

GI number: 159899279

Start: 3514174

End: 3515007

Strand: Reverse

Name: glnH [H]

Synonym: Haur_2760

Alternate gene names: 159899279

Gene position: 3515007-3514174 (Counterclockwise)

Preceding gene: 159899280

Following gene: 159899278

Centisome position: 55.38

GC content: 51.44

Gene sequence:

>834_bases
ATGAAACGCCTCAATGTTGTGGCTCTGTTGAGCTTACTGTTGGTTGTGCTGGGCGCATGTGGCGCAGAGTCAGCAACTCC
AACAACTGCTCCAACAACCGCTTCGACCACCGCTCCCGTCGCTGGCAAGATCGATCTTGCTGGCCGTGAAGTCAGCATCG
CCGTCGAAAACTTGTACCCACCATTCAACTACATCAACCCGCAAACGGGCAAAGGCGAAGGCTGGGATTACGATGCTTGG
ACAGCGATTTGTGCTGAGTTGAACTGTAAACCAGTTTTCAAAGAAACCTCATGGGAAGGCATGATCCAAGCGGTTGGCAA
TGGTCAATTCGACGCTGCTGCCGATGGGATCACGATCACGCCTGAACGAGCTGAGCAAGTGGCTTTCTCCGATGGCTATA
TTCAAATTAGCCAACGCCTGTTGGTGCGCAACGATGAAACTCGTTTCACCAGCATGGACGAATTCGCCAAAACTGAATTC
CGCTTAGGCACGCAAACTGGCACCAGCAACTACGAAACCGCCAAAGGTTTGCTCGATGAAGCCCGCATCCAAGGTTTTGA
AACCTTCCCATTTGCGGTAGCCGCCTTGATCAACGGCGACGTTGACGCGGTGATTATGGACGAAACCGCTGGCCAAGGCT
ATGTTGGCAACAACAGCGACCAATTGAAGCTGGTTGGCGACCCAATCAAGAGCGATCAATTGGGCTTTATTTTTCCAAAG
AACAGCGATTTGGTTGAGCCAGTGAATGCCGCCCTCAAGGCGCTGCGCGAAAATGGCAAGCTAGCTGAGCTTGAAAAGAA
ATATTTCGGCCCTGATTTCAAATTGCCCGAATAA

Upstream 100 bases:

>100_bases
CGCAATAAGCCCTAGCAATTTTTGATTTTAGGCCAAGATTTTGCAAAACAACCGTGCGATTGACGGTATAATACCAACTC
TATATCAGGAGGAACTGCAG

Downstream 100 bases:

>100_bases
GCTCATCAATTCAGTGGTGTGGTGCATTGAGGCGCTACACCACTGGGTTTATTCCATCCTCTCCTTAGGCATCACAAGGA
GTTCCCATGGCTGCCGACCT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKRLNVVALLSLLLVVLGACGAESATPTTAPTTASTTAPVAGKIDLAGREVSIAVENLYPPFNYINPQTGKGEGWDYDAW
TAICAELNCKPVFKETSWEGMIQAVGNGQFDAAADGITITPERAEQVAFSDGYIQISQRLLVRNDETRFTSMDEFAKTEF
RLGTQTGTSNYETAKGLLDEARIQGFETFPFAVAALINGDVDAVIMDETAGQGYVGNNSDQLKLVGDPIKSDQLGFIFPK
NSDLVEPVNAALKALRENGKLAELEKKYFGPDFKLPE

Sequences:

>Translated_277_residues
MKRLNVVALLSLLLVVLGACGAESATPTTAPTTASTTAPVAGKIDLAGREVSIAVENLYPPFNYINPQTGKGEGWDYDAW
TAICAELNCKPVFKETSWEGMIQAVGNGQFDAAADGITITPERAEQVAFSDGYIQISQRLLVRNDETRFTSMDEFAKTEF
RLGTQTGTSNYETAKGLLDEARIQGFETFPFAVAALINGDVDAVIMDETAGQGYVGNNSDQLKLVGDPIKSDQLGFIFPK
NSDLVEPVNAALKALRENGKLAELEKKYFGPDFKLPE
>Mature_277_residues
MKRLNVVALLSLLLVVLGACGAESATPTTAPTTASTTAPVAGKIDLAGREVSIAVENLYPPFNYINPQTGKGEGWDYDAW
TAICAELNCKPVFKETSWEGMIQAVGNGQFDAAADGITITPERAEQVAFSDGYIQISQRLLVRNDETRFTSMDEFAKTEF
RLGTQTGTSNYETAKGLLDEARIQGFETFPFAVAALINGDVDAVIMDETAGQGYVGNNSDQLKLVGDPIKSDQLGFIFPK
NSDLVEPVNAALKALRENGKLAELEKKYFGPDFKLPE

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=32.6612903225806, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=25.6756756756757, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787085, Length=229, Percent_Identity=25.764192139738, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787088, Length=229, Percent_Identity=25.764192139738, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29915; Mature: 29915

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLNVVALLSLLLVVLGACGAESATPTTAPTTASTTAPVAGKIDLAGREVSIAVENLYP
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCC
PFNYINPQTGKGEGWDYDAWTAICAELNCKPVFKETSWEGMIQAVGNGQFDAAADGITIT
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEC
PERAEQVAFSDGYIQISQRLLVRNDETRFTSMDEFAKTEFRLGTQTGTSNYETAKGLLDE
HHHHHHEEECCCHHEEEHHHEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHH
ARIQGFETFPFAVAALINGDVDAVIMDETAGQGYVGNNSDQLKLVGDPIKSDQLGFIFPK
HHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEECC
NSDLVEPVNAALKALRENGKLAELEKKYFGPDFKLPE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKRLNVVALLSLLLVVLGACGAESATPTTAPTTASTTAPVAGKIDLAGREVSIAVENLYP
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCC
PFNYINPQTGKGEGWDYDAWTAICAELNCKPVFKETSWEGMIQAVGNGQFDAAADGITIT
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEC
PERAEQVAFSDGYIQISQRLLVRNDETRFTSMDEFAKTEFRLGTQTGTSNYETAKGLLDE
HHHHHHEEECCCHHEEEHHHEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHH
ARIQGFETFPFAVAALINGDVDAVIMDETAGQGYVGNNSDQLKLVGDPIKSDQLGFIFPK
HHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEECC
NSDLVEPVNAALKALRENGKLAELEKKYFGPDFKLPE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]