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The map label for this gene is rpfG [H]

Identifier: 159899218

GI number: 159899218

Start: 3453139

End: 3454125

Strand: Direct

Name: rpfG [H]

Synonym: Haur_2699

Alternate gene names: 159899218

Gene position: 3453139-3454125 (Clockwise)

Preceding gene: 159899217

Following gene: 159899219

Centisome position: 54.41

GC content: 53.09

Gene sequence:

>987_bases
ATGTCGGAGATCATTTTAGTTGTTGATGATGAGGCTCGTAGTATTGATACCTTGCGACGGGTGCTTGAACGTGAGCGCTA
TACCGTGCGCATCGCTCGTGATGGTGTCGAGGCACTAGCTGACATTCAAGCATGTATGCCTGATTTGGTCTTGCTCGATG
TAACTATGCCCGTTATGAATGGGTTTGAGGTCTGTCGGCGGTTGCGCGACCGCGAGGAAACCCAATTGTTGCCAGTTGCC
ATGCTCACCGGCCTTAACGATGATGAATCGCGGATTTTGGGCTTGGAGATGGGCGCTGATGATTTTATCAACAAGCCGTT
CGAGCGCACTGTGCTCTTAGCTCGCATTCGCTCGCTGCTCCGAGTGCGCCGCCTCACCGAGGAACTTGAAAGCACTGAGC
GGGTGATTTTCACCCTCGCAACCACAGTCGAATCGCGTGATCCCTACACTGAAGGCCACTTGCGCCGACTAGCTTCGTAT
AGTTATGCCTTGGCGACGGCCTGCGGCTGCGATGCCAAAGAGTCGCGTTTGATTCGCTATGGTGGTTTGTTGCATGACAT
CGGCAAAATCGCCATTCGCGAAACGGTGCTGCAAAAAGAGGGCAAACTCACCAGCGAAGAATACGACGAGATGAAGAAGC
ATCCGCAAATTGGGGCCAATATTGTCGCGCCAATGCGCTTTTCGGTTTCGGTTGGGCCAATGGTTTTACACCATCATGAA
CGGTGGGATGGCAAAGGCTACCCGCATGGAATCGCTGGTGATACAATTCCGCTAGGGGCGCGGATCATTGCGGTGGTTGA
TAGCTTCGATGCAATGACGACTGATCGACCTTATCGCAAAGGTATGAGCCGTGCCGAAGCAGTGCAAGTGCTTCAATCGG
GGCGCAACACCCAATGGCAAGCCGAATTGGTCGATCTCTTCGTCGAATTGATCGAACATGGCCATGTGGCTTCGGATGAT
CTTAGTTGGGTGCTGCGCAACGCCTAA

Upstream 100 bases:

>100_bases
ACCATGCAAGCACTGGTTGATGATCAGGCTAAGCATCCGCCGCAATTGCGTTTCGTTTCAGCTTCCGATCCACTTTGCAA
TCAACATTGAGGGTATCGTC

Downstream 100 bases:

>100_bases
ACTCAATGCGCCTTAAACGGGCGTTTCAGTGAGGTAGCTATGCATCCTGATTTGCTTCCATGGTTGCGTTGTCCGGTTTC
ACAAACCAGCCTTAGCCTGC

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSEIILVVDDEARSIDTLRRVLERERYTVRIARDGVEALADIQACMPDLVLLDVTMPVMNGFEVCRRLRDREETQLLPVA
MLTGLNDDESRILGLEMGADDFINKPFERTVLLARIRSLLRVRRLTEELESTERVIFTLATTVESRDPYTEGHLRRLASY
SYALATACGCDAKESRLIRYGGLLHDIGKIAIRETVLQKEGKLTSEEYDEMKKHPQIGANIVAPMRFSVSVGPMVLHHHE
RWDGKGYPHGIAGDTIPLGARIIAVVDSFDAMTTDRPYRKGMSRAEAVQVLQSGRNTQWQAELVDLFVELIEHGHVASDD
LSWVLRNA

Sequences:

>Translated_328_residues
MSEIILVVDDEARSIDTLRRVLERERYTVRIARDGVEALADIQACMPDLVLLDVTMPVMNGFEVCRRLRDREETQLLPVA
MLTGLNDDESRILGLEMGADDFINKPFERTVLLARIRSLLRVRRLTEELESTERVIFTLATTVESRDPYTEGHLRRLASY
SYALATACGCDAKESRLIRYGGLLHDIGKIAIRETVLQKEGKLTSEEYDEMKKHPQIGANIVAPMRFSVSVGPMVLHHHE
RWDGKGYPHGIAGDTIPLGARIIAVVDSFDAMTTDRPYRKGMSRAEAVQVLQSGRNTQWQAELVDLFVELIEHGHVASDD
LSWVLRNA
>Mature_327_residues
SEIILVVDDEARSIDTLRRVLERERYTVRIARDGVEALADIQACMPDLVLLDVTMPVMNGFEVCRRLRDREETQLLPVAM
LTGLNDDESRILGLEMGADDFINKPFERTVLLARIRSLLRVRRLTEELESTERVIFTLATTVESRDPYTEGHLRRLASYS
YALATACGCDAKESRLIRYGGLLHDIGKIAIRETVLQKEGKLTSEEYDEMKKHPQIGANIVAPMRFSVSVGPMVLHHHER
WDGKGYPHGIAGDTIPLGARIIAVVDSFDAMTTDRPYRKGMSRAEAVQVLQSGRNTQWQAELVDLFVELIEHGHVASDDL
SWVLRNA

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=119, Percent_Identity=39.4957983193277, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=38.8429752066116, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=36.7521367521368, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790863, Length=207, Percent_Identity=31.4009661835749, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789809, Length=176, Percent_Identity=32.9545454545455, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI2367329, Length=135, Percent_Identity=40.7407407407407, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788394, Length=129, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=36.6666666666667, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=32.4786324786325, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790552, Length=162, Percent_Identity=32.0987654320988, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37040; Mature: 36909

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIILVVDDEARSIDTLRRVLERERYTVRIARDGVEALADIQACMPDLVLLDVTMPVMN
CCCEEEEECCCCHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GFEVCRRLRDREETQLLPVAMLTGLNDDESRILGLEMGADDFINKPFERTVLLARIRSLL
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHH
RVRRLTEELESTERVIFTLATTVESRDPYTEGHLRRLASYSYALATACGCDAKESRLIRY
HHHHHHHHHHHHHHEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GGLLHDIGKIAIRETVLQKEGKLTSEEYDEMKKHPQIGANIVAPMRFSVSVGPMVLHHHE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEECEEEEEECCCHHEECCC
RWDGKGYPHGIAGDTIPLGARIIAVVDSFDAMTTDRPYRKGMSRAEAVQVLQSGRNTQWQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHCCCCCCHH
AELVDLFVELIEHGHVASDDLSWVLRNA
HHHHHHHHHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SEIILVVDDEARSIDTLRRVLERERYTVRIARDGVEALADIQACMPDLVLLDVTMPVMN
CCEEEEECCCCHHHHHHHHHHHCCHHEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GFEVCRRLRDREETQLLPVAMLTGLNDDESRILGLEMGADDFINKPFERTVLLARIRSLL
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHH
RVRRLTEELESTERVIFTLATTVESRDPYTEGHLRRLASYSYALATACGCDAKESRLIRY
HHHHHHHHHHHHHHEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GGLLHDIGKIAIRETVLQKEGKLTSEEYDEMKKHPQIGANIVAPMRFSVSVGPMVLHHHE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEECEEEEEECCCHHEECCC
RWDGKGYPHGIAGDTIPLGARIIAVVDSFDAMTTDRPYRKGMSRAEAVQVLQSGRNTQWQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHCCCCCCHH
AELVDLFVELIEHGHVASDDLSWVLRNA
HHHHHHHHHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]