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The map label for this gene is pgaC [H]

Identifier: 159899208

GI number: 159899208

Start: 3446848

End: 3447843

Strand: Reverse

Name: pgaC [H]

Synonym: Haur_2689

Alternate gene names: 159899208

Gene position: 3447843-3446848 (Counterclockwise)

Preceding gene: 159899209

Following gene: 159899201

Centisome position: 54.33

GC content: 54.52

Gene sequence:

>996_bases
ATGAATTGTTCTATCATTATTTTAAATTGGAATGGCCGAGCACTGCTCGCTGATTGCCTCAACGCCTTATTGCCCCAATG
CGATGCTTCAATCGAAGTGTTGGTGGTGGATAATGGCTCACATGATGGCTCGGCGGCGTGGCTGCATCAACATTATCCCC
AAGTGCGCTTGTTGGCGCTCACCAACAATCGTGGATTTAGCGGTGGGGTCAATGTTGGCTTGCATGTGGCGCGTGGCGAT
GTGCTGTTGTTGTTGAATAATGATGCAATCGTTGAGCCAAATTTTATCTCGGCGATTCTCGCGCCGTTTCAGCACCAACC
AACGCTTGCTGCTAGCGCTGGTCTGATGACGTTCGCGCATCGACCTGAAATCATCGCCTCAGCCGGAATTCAGCTCTATC
GCGATGGCGTGGCAACTGATGCTGGGTTGTTGCAGCCAGTTGCTCAATTAGCCAGCCAACCAAGCCCAATTTGGGGTGGC
AGCGGTGGAGCGGTAGCCTATCGACGTGCCGCTTTAGCCGATGTCGGCATGTTCGATGAAGGCTATTTTGCCTATTTGGA
AGATGTCGATTTGGCTTGGCGTTTGCAGTTGCGCGAATGGCAAACCGTGCTAGCCCCGCAGGCCGTCGCTCGGCATATCT
ACTCGGCCACTGGCGGCGAAGGTTCGCCCTTTAAGGATTGGCTGATTGCGCGTAATCGCTGGCGGGTAATTTTGCGTTGC
TGGCCAACGCCGTTGTTGGCCCGCGCTCTCCCATTAATGCTAGCTTACGATGGTTTGGCTTGTGCACAGGCTATCGTGCG
CCGCCGCTGGACAACGGTCAGCGGGCGTTTGCATGCCTTGCGCCAACTGCCCCAACTACGCCAACAGCGCCAAGCAATTC
AAGCTCGCCGCACGGCTAGCATCGCTGAGCTTGATCATTGGATTAAACCAGCCCGTTCACCACTGGCAATTTGGCGTGAA
AATCAAGCACTCGGCCAATTGATCGCTCAACGCTAG

Upstream 100 bases:

>100_bases
TGGACTCAACGCCCAATTGGCGGCGTTGGCTGGAAGTGTTATTTGATTCTTAATTTCGGATGAAGGATCGAGCGATTGGG
TAACCAATCGCTTTTTTTGT

Downstream 100 bases:

>100_bases
CCGTTGCTGGCCTCGATCATTCGTTGCATACGCTGATTGGCGCTGCCAGTGTCGATGGCCTGTTGAGCTTGACGCAAAGC
CTCGCCAAACGAATCGGCGC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNCSIIILNWNGRALLADCLNALLPQCDASIEVLVVDNGSHDGSAAWLHQHYPQVRLLALTNNRGFSGGVNVGLHVARGD
VLLLLNNDAIVEPNFISAILAPFQHQPTLAASAGLMTFAHRPEIIASAGIQLYRDGVATDAGLLQPVAQLASQPSPIWGG
SGGAVAYRRAALADVGMFDEGYFAYLEDVDLAWRLQLREWQTVLAPQAVARHIYSATGGEGSPFKDWLIARNRWRVILRC
WPTPLLARALPLMLAYDGLACAQAIVRRRWTTVSGRLHALRQLPQLRQQRQAIQARRTASIAELDHWIKPARSPLAIWRE
NQALGQLIAQR

Sequences:

>Translated_331_residues
MNCSIIILNWNGRALLADCLNALLPQCDASIEVLVVDNGSHDGSAAWLHQHYPQVRLLALTNNRGFSGGVNVGLHVARGD
VLLLLNNDAIVEPNFISAILAPFQHQPTLAASAGLMTFAHRPEIIASAGIQLYRDGVATDAGLLQPVAQLASQPSPIWGG
SGGAVAYRRAALADVGMFDEGYFAYLEDVDLAWRLQLREWQTVLAPQAVARHIYSATGGEGSPFKDWLIARNRWRVILRC
WPTPLLARALPLMLAYDGLACAQAIVRRRWTTVSGRLHALRQLPQLRQQRQAIQARRTASIAELDHWIKPARSPLAIWRE
NQALGQLIAQR
>Mature_331_residues
MNCSIIILNWNGRALLADCLNALLPQCDASIEVLVVDNGSHDGSAAWLHQHYPQVRLLALTNNRGFSGGVNVGLHVARGD
VLLLLNNDAIVEPNFISAILAPFQHQPTLAASAGLMTFAHRPEIIASAGIQLYRDGVATDAGLLQPVAQLASQPSPIWGG
SGGAVAYRRAALADVGMFDEGYFAYLEDVDLAWRLQLREWQTVLAPQAVARHIYSATGGEGSPFKDWLIARNRWRVILRC
WPTPLLARALPLMLAYDGLACAQAIVRRRWTTVSGRLHALRQLPQLRQQRQAIQARRTASIAELDHWIKPARSPLAIWRE
NQALGQLIAQR

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1216

COG function: function code R; Predicted glycosyltransferases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=210, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 36409; Mature: 36409

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCSIIILNWNGRALLADCLNALLPQCDASIEVLVVDNGSHDGSAAWLHQHYPQVRLLAL
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEE
TNNRGFSGGVNVGLHVARGDVLLLLNNDAIVEPNFISAILAPFQHQPTLAASAGLMTFAH
ECCCCCCCCCCEEEEEECCCEEEEECCCCEECCHHHHHHHHCCCCCCCHHHHCCHHHHCC
RPEIIASAGIQLYRDGVATDAGLLQPVAQLASQPSPIWGGSGGAVAYRRAALADVGMFDE
CCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
GYFAYLEDVDLAWRLQLREWQTVLAPQAVARHIYSATGGEGSPFKDWLIARNRWRVILRC
CCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEEE
WPTPLLARALPLMLAYDGLACAQAIVRRRWTTVSGRLHALRQLPQLRQQRQAIQARRTAS
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAELDHWIKPARSPLAIWRENQALGQLIAQR
HHHHHHHHCCCCCCHHEEECCHHHHHHHHCC
>Mature Secondary Structure
MNCSIIILNWNGRALLADCLNALLPQCDASIEVLVVDNGSHDGSAAWLHQHYPQVRLLAL
CCEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEE
TNNRGFSGGVNVGLHVARGDVLLLLNNDAIVEPNFISAILAPFQHQPTLAASAGLMTFAH
ECCCCCCCCCCEEEEEECCCEEEEECCCCEECCHHHHHHHHCCCCCCCHHHHCCHHHHCC
RPEIIASAGIQLYRDGVATDAGLLQPVAQLASQPSPIWGGSGGAVAYRRAALADVGMFDE
CCHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
GYFAYLEDVDLAWRLQLREWQTVLAPQAVARHIYSATGGEGSPFKDWLIARNRWRVILRC
CCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEEE
WPTPLLARALPLMLAYDGLACAQAIVRRRWTTVSGRLHALRQLPQLRQQRQAIQARRTAS
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAELDHWIKPARSPLAIWRENQALGQLIAQR
HHHHHHHHCCCCCCHHEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]