Click here to switch to the map view.

The map label for this gene is nucA [H]

Identifier: 159899190

GI number: 159899190

Start: 3424400

End: 3425812

Strand: Direct

Name: nucA [H]

Synonym: Haur_2671

Alternate gene names: 159899190

Gene position: 3424400-3425812 (Clockwise)

Preceding gene: 159899182

Following gene: 159899191

Centisome position: 53.96

GC content: 54.0

Gene sequence:

>1413_bases
ATGAAGGATCGTCGGCTGATTACCCTGCTCGCGTTGTTCGTCCTTGGGTTTGTTGGGGCTAGCTTGGTACAACCAACGCA
GGCCAAAACCGTTAGTAGTGATAACTTGGTCTTGGGCAATCCCAGCGGGGCCGTCGCCAGCAGCAGCTATCCGACCAATT
ATTTAATTCAACGCAACCAATATGCGCTCTCGTATCACCGCGATAATGGGATTGCCAATTGGGTCAGTTGGCACCTCGAT
AGCGGCGATATTGGTAGCGTTTCACGCAGCGATTTTCAAACCGACACTAGCTTGCCCAGTGGTTGGTATCGCGTTGCCAC
TGGCGATTACAGCGGCAGCGGCTATGATCGCGGCCATATGACTCCCTCAGGCGATCGCACCGCCACCACCGCCGATAACC
AAGCCACCTTCTACATGACCAACATTATTCCCCAAGCGCCCGATAACAACCAAGGCCCATGGGTTGACCTCGAAACCTAT
GCTCGCGAGTTGGTCAGCGCTGGCAACGAGTTGTATATTATCAGCGGCGGGGCTGGCTCACGTGGCACAATCGCCAGTGG
CAAGGTGCGAATTCCGAATTCAACCTGGAAAATCATCGTCGTGCTTAGCCAAGGCAGTAACGACCTCAGCCGCGTCAGCA
ACAACACCCGCGTCATCGCGATCAACATGCCCAATGTGCAAGGTATTCGCGATAACGATTGGCGCGATTATCTGACCACG
GTTGATGCTCTCGAAAGCTTGACGGGCTATAACTTCCTTTCAAATGTCTCAACCAACATCCAAAATGTGATTGAAGCCCG
CGTCGATGGCTCGACCACACCAATTCCGACAGCTGTACCAACCTCGGGAACCAACCCAACCGCCACTCCAGTACGCACGG
CCACCCCAACCCCCAGCACCGGCTGTACATCGAGCCGCCTGTTCTTCTCAGAATATGTCGAAGGCAGCAGCAACAACAAA
GCTTTGGAACTTTACAATAATACTGGAGCCAGCGTCAGCCTCAGTGGCTATAGCATTCAGTTGTATGCCAACGGCTCGAC
CAGCGCCAGCAGTAGCGTGAATTTGAGTGGCTCGGTCGCCAATGGCGCAACCTATGTGATTGCCAACGCCTCGGCATCAA
GTAGCGTACAGAATCTTGCTAACATCACCAGCAGTGTGGCCAACTTCAATGGCAATGATGCACTTGTGCTGACCTACAAT
GGCACGGTGGTTGATAGCTTTGGCCAAGTTGGCAACGACCCAGGTAGCAGCGGTTGGGGTGGCACAACCACCGATCGCAC
GTTGCGCCGTAAAGCAACAATCAGCGCAGGCGATACCAATCGCAGCGATAGCTTCACCCCAAGCAGCACCTGGGATAGCT
ATAGCCTTGATACATTCAGTGGCTTGGGCAACCACAGTGTCAGTTGCCCATAG

Upstream 100 bases:

>100_bases
TAATAAATTATTAAGTATGGTATTCTATAGTACCAAACTAGGAACAAAGAATAACGAAACGCCACATCAGATCAGCAATT
CCATTCTGAAAGGAAGTTAC

Downstream 100 bases:

>100_bases
GCCGCAAATCGTGCTGAATTGAATATCTGCAAAGGGCTGCTGGAGGAAAATCTAGCAGCCCTTTCATTCTTAAGGACGAG
GGCGGTTCGCTCTCGAATAC

Product: DNA/RNA non-specific endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKDRRLITLLALFVLGFVGASLVQPTQAKTVSSDNLVLGNPSGAVASSSYPTNYLIQRNQYALSYHRDNGIANWVSWHLD
SGDIGSVSRSDFQTDTSLPSGWYRVATGDYSGSGYDRGHMTPSGDRTATTADNQATFYMTNIIPQAPDNNQGPWVDLETY
ARELVSAGNELYIISGGAGSRGTIASGKVRIPNSTWKIIVVLSQGSNDLSRVSNNTRVIAINMPNVQGIRDNDWRDYLTT
VDALESLTGYNFLSNVSTNIQNVIEARVDGSTTPIPTAVPTSGTNPTATPVRTATPTPSTGCTSSRLFFSEYVEGSSNNK
ALELYNNTGASVSLSGYSIQLYANGSTSASSSVNLSGSVANGATYVIANASASSSVQNLANITSSVANFNGNDALVLTYN
GTVVDSFGQVGNDPGSSGWGGTTTDRTLRRKATISAGDTNRSDSFTPSSTWDSYSLDTFSGLGNHSVSCP

Sequences:

>Translated_470_residues
MKDRRLITLLALFVLGFVGASLVQPTQAKTVSSDNLVLGNPSGAVASSSYPTNYLIQRNQYALSYHRDNGIANWVSWHLD
SGDIGSVSRSDFQTDTSLPSGWYRVATGDYSGSGYDRGHMTPSGDRTATTADNQATFYMTNIIPQAPDNNQGPWVDLETY
ARELVSAGNELYIISGGAGSRGTIASGKVRIPNSTWKIIVVLSQGSNDLSRVSNNTRVIAINMPNVQGIRDNDWRDYLTT
VDALESLTGYNFLSNVSTNIQNVIEARVDGSTTPIPTAVPTSGTNPTATPVRTATPTPSTGCTSSRLFFSEYVEGSSNNK
ALELYNNTGASVSLSGYSIQLYANGSTSASSSVNLSGSVANGATYVIANASASSSVQNLANITSSVANFNGNDALVLTYN
GTVVDSFGQVGNDPGSSGWGGTTTDRTLRRKATISAGDTNRSDSFTPSSTWDSYSLDTFSGLGNHSVSCP
>Mature_470_residues
MKDRRLITLLALFVLGFVGASLVQPTQAKTVSSDNLVLGNPSGAVASSSYPTNYLIQRNQYALSYHRDNGIANWVSWHLD
SGDIGSVSRSDFQTDTSLPSGWYRVATGDYSGSGYDRGHMTPSGDRTATTADNQATFYMTNIIPQAPDNNQGPWVDLETY
ARELVSAGNELYIISGGAGSRGTIASGKVRIPNSTWKIIVVLSQGSNDLSRVSNNTRVIAINMPNVQGIRDNDWRDYLTT
VDALESLTGYNFLSNVSTNIQNVIEARVDGSTTPIPTAVPTSGTNPTATPVRTATPTPSTGCTSSRLFFSEYVEGSSNNK
ALELYNNTGASVSLSGYSIQLYANGSTSASSSVNLSGSVANGATYVIANASASSSVQNLANITSSVANFNGNDALVLTYN
GTVVDSFGQVGNDPGSSGWGGTTTDRTLRRKATISAGDTNRSDSFTPSSTWDSYSLDTFSGLGNHSVSCP

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI224451073, Length=224, Percent_Identity=28.125, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI53759134, Length=217, Percent_Identity=27.1889400921659, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI224451075, Length=217, Percent_Identity=26.2672811059908, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322253, Length=238, Percent_Identity=24.7899159663866, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581065, Length=213, Percent_Identity=29.1079812206573, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20129899, Length=216, Percent_Identity=28.2407407407407, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: NA

Molecular weight: Translated: 49633; Mature: 49633

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRRLITLLALFVLGFVGASLVQPTQAKTVSSDNLVLGNPSGAVASSSYPTNYLIQRNQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCCCCCCCCEEEECCC
YALSYHRDNGIANWVSWHLDSGDIGSVSRSDFQTDTSLPSGWYRVATGDYSGSGYDRGHM
EEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TPSGDRTATTADNQATFYMTNIIPQAPDNNQGPWVDLETYARELVSAGNELYIISGGAGS
CCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCCCC
RGTIASGKVRIPNSTWKIIVVLSQGSNDLSRVSNNTRVIAINMPNVQGIRDNDWRDYLTT
CCCCCCCEEECCCCCEEEEEEEECCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHH
VDALESLTGYNFLSNVSTNIQNVIEARVDGSTTPIPTAVPTSGTNPTATPVRTATPTPST
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC
GCTSSRLFFSEYVEGSSNNKALELYNNTGASVSLSGYSIQLYANGSTSASSSVNLSGSVA
CCCCCHHHHHHHHCCCCCCCEEEEEECCCCEEEECCEEEEEEECCCCCCCCEEEECCEEC
NGATYVIANASASSSVQNLANITSSVANFNGNDALVLTYNGTVVDSFGQVGNDPGSSGWG
CCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECHHHCCCCCCCCCCC
GTTTDRTLRRKATISAGDTNRSDSFTPSSTWDSYSLDTFSGLGNHSVSCP
CCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MKDRRLITLLALFVLGFVGASLVQPTQAKTVSSDNLVLGNPSGAVASSSYPTNYLIQRNQ
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCCCCCCCCEEEECCC
YALSYHRDNGIANWVSWHLDSGDIGSVSRSDFQTDTSLPSGWYRVATGDYSGSGYDRGHM
EEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TPSGDRTATTADNQATFYMTNIIPQAPDNNQGPWVDLETYARELVSAGNELYIISGGAGS
CCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCCCC
RGTIASGKVRIPNSTWKIIVVLSQGSNDLSRVSNNTRVIAINMPNVQGIRDNDWRDYLTT
CCCCCCCEEECCCCCEEEEEEEECCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHH
VDALESLTGYNFLSNVSTNIQNVIEARVDGSTTPIPTAVPTSGTNPTATPVRTATPTPST
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCC
GCTSSRLFFSEYVEGSSNNKALELYNNTGASVSLSGYSIQLYANGSTSASSSVNLSGSVA
CCCCCHHHHHHHHCCCCCCCEEEEEECCCCEEEECCEEEEEEECCCCCCCCEEEECCEEC
NGATYVIANASASSSVQNLANITSSVANFNGNDALVLTYNGTVVDSFGQVGNDPGSSGWG
CCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECHHHCCCCCCCCCCC
GTTTDRTLRRKATISAGDTNRSDSFTPSSTWDSYSLDTFSGLGNHSVSCP
CCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]