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The map label for this gene is appA [H]

Identifier: 159899176

GI number: 159899176

Start: 3410085

End: 3411938

Strand: Direct

Name: appA [H]

Synonym: Haur_2657

Alternate gene names: 159899176

Gene position: 3410085-3411938 (Clockwise)

Preceding gene: 159899175

Following gene: 159899177

Centisome position: 53.73

GC content: 49.95

Gene sequence:

>1854_bases
ATGTTCGAACGAAGCAAACGCTTAGCCGCCATCGTGCTTACTGGCGCGATTTTGGCAGCATGCGGTAGCGGCACCAGCAC
CACACAACCAACCACTGGCAGCAGCGAGGCAACCGCCGTCGTTGAGCCAGGTACTGATACTGGGTCGCAACCCAGCAGCG
ATGGCATTGTAATCATTGGGATGAGCCAATCGCCAGACACCTTATTTGGGATGGAATCGCAATCGAGCGCCACCACCCAA
GTGTTAAACTCAGTGCAACCAGCCTGTGTAACGACCCTCAGCTACGATTATCAACCAGTCTGTTTCGCTGAATTGCCCAC
CTTTGAAAATGGCGGCGCAGTCGAAGAAATGGTAACAGTTGATCAAAGCTATACCGGCCCATTCGTGATCGAGAATGAAC
TGATCACTGATACCAGCGTTTTGACTGGGCCAATCGAATTGCCCCAAGTTAAAGTGACATGGAAGTTGATCGATGGCATT
ACTTGGGAAGATGGCACACCAGTAACCGCCGATGACTTTTTGTTTGCTGCTGAATTGTATGCTAACCCAGGCACCAAAGT
CGCCAGCCGCTTCACCATCGAGCACACCGCCAAATACGAAAAAGTTGATGATCAAACTTTCTCATGGTATGGCGTGCCAG
GCTATCAAGATTCAACCTATTTCTTGAACTACGCAGGTGGCGCACTTGGCCCTGAACCCAAGCATGTGCTCGGCAGCGTC
GATGCAGCAACCATCGGCGGTAGCGATTATTCCAGCAAACCGTTAGCTTATGGCCCGTACAAAATTGACGAATATGTGCC
GCAAGAACGGGTGACAATGAGTGCTAATCCACATTATTGGGGAGCAAAGCAAGGCCTACCCAAAATTAGCAATGTAATCT
ATAAATTCGTCACCAGCGAAGATCAAATTTTGCAACAATTGCAATCAGGCGAAATCGATGTCGTTGGCCAAATTGGCTTA
TCGCTAGCTCAAGCGCCATCACTTGATGAATTGCAAGCAGGCGCTGAATTCGATATTCAATATGTGCCAGCCACGGTTTG
GGAACATATTGACTTTGCTGTTGAGCGCGGCGATGGCGTTGCAACCCCATTTGCTGATGCCAAAGTGCGTCAAGCAGTCG
CCTATGCAATCAACCGCCAAGAAATCATCGATCAGATCTTGTTTGGTAAAACCGTCGCAATGAACAGCTTCATGCCCGAT
GATCACTGGGCCTATCCCAGCGATGCCAGCGTGATCAACTCTTATGCCTTTGATCCCAGCAAAGCGATTCAATTGCTGAA
TGAAGCTGGTTGGGTTGCTGGCGATGATGGCATTTTGGTCAAAGATGGCGAACCATTCAAGGTTGAATTCTTCACCACCG
AAGGCAACGATACCCGTCAAGCAGTCGCCCAATTGGTGCAAGAATACTTGCGCGATGTCGGGATCGACGTTGAATTAAAG
TTTGTCGCTGGCACTGATGTGCTGTTCAAAAATGGCTCGGAAGGTATTTTGGCAGGTCGCCGCTACGATATGGCATTGTA
TGCTTGGGTCAGTGGCCCTGAGCCATCAACGCCGTTGTATCTGTGCAGCCAAGTGCCAACAGCAGCCAATGGCTACGCTG
GCCAAAACAACACTGGCTACTGCAACCCCGATTACGATAAAGTAGCGCTCGAAAGCCAAAGCATCATCGAACGGGCTGGC
CGCTTGCCATTGCTTAGCCAAGCCCAACAAATCTTCAACCGCGATTTGCCAACCTTGCCGTTATATCAACGGATCAACGT
TGGGGCTGCCCGCAAAACAATCAGCGGCTTCAAGCTCGATCCAACCAGCCAACAAGATTTCTACAACATCGAAACCTGGG
AATTAGCCCAATAA

Upstream 100 bases:

>100_bases
CAGCGAAAAAAAGCGAGGCGTGCGTTCCGGGAAGACGCAGGCGCAGCTTTTTTCGTTTTTAGTTGGCAGATTTTTGGATA
CAGGAAAGAGGATTCCAGTC

Downstream 100 bases:

>100_bases
CGTTTTGATCCGTTAACCACGAAGAGCACGAAGGATCAGCTTTCGTGCTCTTTACGATTAATGACCCATGAAGGACACGA
AGAGCACAAAGGCTTTAATT

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MFERSKRLAAIVLTGAILAACGSGTSTTQPTTGSSEATAVVEPGTDTGSQPSSDGIVIIGMSQSPDTLFGMESQSSATTQ
VLNSVQPACVTTLSYDYQPVCFAELPTFENGGAVEEMVTVDQSYTGPFVIENELITDTSVLTGPIELPQVKVTWKLIDGI
TWEDGTPVTADDFLFAAELYANPGTKVASRFTIEHTAKYEKVDDQTFSWYGVPGYQDSTYFLNYAGGALGPEPKHVLGSV
DAATIGGSDYSSKPLAYGPYKIDEYVPQERVTMSANPHYWGAKQGLPKISNVIYKFVTSEDQILQQLQSGEIDVVGQIGL
SLAQAPSLDELQAGAEFDIQYVPATVWEHIDFAVERGDGVATPFADAKVRQAVAYAINRQEIIDQILFGKTVAMNSFMPD
DHWAYPSDASVINSYAFDPSKAIQLLNEAGWVAGDDGILVKDGEPFKVEFFTTEGNDTRQAVAQLVQEYLRDVGIDVELK
FVAGTDVLFKNGSEGILAGRRYDMALYAWVSGPEPSTPLYLCSQVPTAANGYAGQNNTGYCNPDYDKVALESQSIIERAG
RLPLLSQAQQIFNRDLPTLPLYQRINVGAARKTISGFKLDPTSQQDFYNIETWELAQ

Sequences:

>Translated_617_residues
MFERSKRLAAIVLTGAILAACGSGTSTTQPTTGSSEATAVVEPGTDTGSQPSSDGIVIIGMSQSPDTLFGMESQSSATTQ
VLNSVQPACVTTLSYDYQPVCFAELPTFENGGAVEEMVTVDQSYTGPFVIENELITDTSVLTGPIELPQVKVTWKLIDGI
TWEDGTPVTADDFLFAAELYANPGTKVASRFTIEHTAKYEKVDDQTFSWYGVPGYQDSTYFLNYAGGALGPEPKHVLGSV
DAATIGGSDYSSKPLAYGPYKIDEYVPQERVTMSANPHYWGAKQGLPKISNVIYKFVTSEDQILQQLQSGEIDVVGQIGL
SLAQAPSLDELQAGAEFDIQYVPATVWEHIDFAVERGDGVATPFADAKVRQAVAYAINRQEIIDQILFGKTVAMNSFMPD
DHWAYPSDASVINSYAFDPSKAIQLLNEAGWVAGDDGILVKDGEPFKVEFFTTEGNDTRQAVAQLVQEYLRDVGIDVELK
FVAGTDVLFKNGSEGILAGRRYDMALYAWVSGPEPSTPLYLCSQVPTAANGYAGQNNTGYCNPDYDKVALESQSIIERAG
RLPLLSQAQQIFNRDLPTLPLYQRINVGAARKTISGFKLDPTSQQDFYNIETWELAQ
>Mature_617_residues
MFERSKRLAAIVLTGAILAACGSGTSTTQPTTGSSEATAVVEPGTDTGSQPSSDGIVIIGMSQSPDTLFGMESQSSATTQ
VLNSVQPACVTTLSYDYQPVCFAELPTFENGGAVEEMVTVDQSYTGPFVIENELITDTSVLTGPIELPQVKVTWKLIDGI
TWEDGTPVTADDFLFAAELYANPGTKVASRFTIEHTAKYEKVDDQTFSWYGVPGYQDSTYFLNYAGGALGPEPKHVLGSV
DAATIGGSDYSSKPLAYGPYKIDEYVPQERVTMSANPHYWGAKQGLPKISNVIYKFVTSEDQILQQLQSGEIDVVGQIGL
SLAQAPSLDELQAGAEFDIQYVPATVWEHIDFAVERGDGVATPFADAKVRQAVAYAINRQEIIDQILFGKTVAMNSFMPD
DHWAYPSDASVINSYAFDPSKAIQLLNEAGWVAGDDGILVKDGEPFKVEFFTTEGNDTRQAVAQLVQEYLRDVGIDVELK
FVAGTDVLFKNGSEGILAGRRYDMALYAWVSGPEPSTPLYLCSQVPTAANGYAGQNNTGYCNPDYDKVALESQSIIERAG
RLPLLSQAQQIFNRDLPTLPLYQRINVGAARKTISGFKLDPTSQQDFYNIETWELAQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=361, Percent_Identity=21.0526315789474, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787052, Length=457, Percent_Identity=23.8512035010941, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787551, Length=360, Percent_Identity=23.3333333333333, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787762, Length=466, Percent_Identity=22.9613733905579, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789887, Length=231, Percent_Identity=26.8398268398268, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66981; Mature: 66981

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERSKRLAAIVLTGAILAACGSGTSTTQPTTGSSEATAVVEPGTDTGSQPSSDGIVIIG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEE
MSQSPDTLFGMESQSSATTQVLNSVQPACVTTLSYDYQPVCFAELPTFENGGAVEEMVTV
CCCCCCCEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHHHHH
DQSYTGPFVIENELITDTSVLTGPIELPQVKVTWKLIDGITWEDGTPVTADDFLFAAELY
CCCCCCCEEEECCEECCCCEEECCCCCCEEEEEEEEECCEECCCCCCCCHHHHEEEEHHH
ANPGTKVASRFTIEHTAKYEKVDDQTFSWYGVPGYQDSTYFLNYAGGALGPEPKHVLGSV
CCCCCHHHHEEEEHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHCCC
DAATIGGSDYSSKPLAYGPYKIDEYVPQERVTMSANPHYWGAKQGLPKISNVIYKFVTSE
CEEEECCCCCCCCCCEECCEEHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCH
DQILQQLQSGEIDVVGQIGLSLAQAPSLDELQAGAEFDIQYVPATVWEHIDFAVERGDGV
HHHHHHHHCCCEEEEEHHCHHHHCCCCHHHHHCCCCEEEEEECHHHHHHHHHEEECCCCC
ATPFADAKVRQAVAYAINRQEIIDQILFGKTVAMNSFMPDDHWAYPSDASVINSYAFDPS
CCCCCHHHHHHHHHHHHCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCHH
KAIQLLNEAGWVAGDDGILVKDGEPFKVEFFTTEGNDTRQAVAQLVQEYLRDVGIDVELK
HHHHHHHHCCCEECCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
FVAGTDVLFKNGSEGILAGRRYDMALYAWVSGPEPSTPLYLCSQVPTAANGYAGQNNTGY
EEECCEEEEECCCCCEEECCEECEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCC
CNPDYDKVALESQSIIERAGRLPLLSQAQQIFNRDLPTLPLYQRINVGAARKTISGFKLD
CCCCHHHEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHCCCCCCC
PTSQQDFYNIETWELAQ
CCCCCCCCCCCEEECCC
>Mature Secondary Structure
MFERSKRLAAIVLTGAILAACGSGTSTTQPTTGSSEATAVVEPGTDTGSQPSSDGIVIIG
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEE
MSQSPDTLFGMESQSSATTQVLNSVQPACVTTLSYDYQPVCFAELPTFENGGAVEEMVTV
CCCCCCCEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHHHHH
DQSYTGPFVIENELITDTSVLTGPIELPQVKVTWKLIDGITWEDGTPVTADDFLFAAELY
CCCCCCCEEEECCEECCCCEEECCCCCCEEEEEEEEECCEECCCCCCCCHHHHEEEEHHH
ANPGTKVASRFTIEHTAKYEKVDDQTFSWYGVPGYQDSTYFLNYAGGALGPEPKHVLGSV
CCCCCHHHHEEEEHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHCCC
DAATIGGSDYSSKPLAYGPYKIDEYVPQERVTMSANPHYWGAKQGLPKISNVIYKFVTSE
CEEEECCCCCCCCCCEECCEEHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCH
DQILQQLQSGEIDVVGQIGLSLAQAPSLDELQAGAEFDIQYVPATVWEHIDFAVERGDGV
HHHHHHHHCCCEEEEEHHCHHHHCCCCHHHHHCCCCEEEEEECHHHHHHHHHEEECCCCC
ATPFADAKVRQAVAYAINRQEIIDQILFGKTVAMNSFMPDDHWAYPSDASVINSYAFDPS
CCCCCHHHHHHHHHHHHCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHCCCCHH
KAIQLLNEAGWVAGDDGILVKDGEPFKVEFFTTEGNDTRQAVAQLVQEYLRDVGIDVELK
HHHHHHHHCCCEECCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
FVAGTDVLFKNGSEGILAGRRYDMALYAWVSGPEPSTPLYLCSQVPTAANGYAGQNNTGY
EEECCEEEEECCCCCEEECCEECEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCC
CNPDYDKVALESQSIIERAGRLPLLSQAQQIFNRDLPTLPLYQRINVGAARKTISGFKLD
CCCCHHHEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHCCCCCCC
PTSQQDFYNIETWELAQ
CCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]