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The map label for this gene is cca [H]

Identifier: 159899134

GI number: 159899134

Start: 3353614

End: 3354828

Strand: Reverse

Name: cca [H]

Synonym: Haur_2615

Alternate gene names: 159899134

Gene position: 3354828-3353614 (Counterclockwise)

Preceding gene: 159899135

Following gene: 159899133

Centisome position: 52.86

GC content: 51.03

Gene sequence:

>1215_bases
ATGTTGTATGATTTGAATCCTTTTTTACGCCAAGCAAGCGCTGAATTACAACAACGTTTACAATGGTTGATCCAAACTGC
CGATACTCAGGGGTTAGCCTGCTATCTGGTCGGTGGTTGTGTGCGCGATTGGTTGCTAGAGCGACCAGTTGGCGATATTG
ATATTGTGGTTGAAGGCGACGCGATTATGCTGGCCGAGATTTGGGCCACCGTTTGGCCAGATCAACTGCATATCCACCCG
CCCTTTGGCACCGCTACCCTGAGTTGGCAGGATGCAGCTGGTGTTTGCAGCTTGGATTTAATTTCAGCCCGCAGTGAATA
CTACCCCCAACCGGCGGCCTTACCAGTCGTTAGTTTTGCCAATTTAGCTGCTGATTTGGCCCGCCGCGATTTTACGATCA
ATTGTCTGGCTTTACGCCTCGCCAACCAGCCAACCCTATTAATTGATCCACAGCATGGTTATGCTGATCTCCAACAGAGT
TTGATTCGGGTGTTGCATGATCAGAGTTTCATCGATGACCCAACCCGCATTCTCCGTGCGATCCGCTTTGCTGCCCGTTT
GAATTTTGAACTTGAACCGCAGACCGCTACACTGTTGCAGCAAGCCTTGCCATGGCTTGATCAAACTACACCTGCGCGTT
TGTGGAATGAGCTAGGTTTGTTGCTCACCGAGCCAAGCGCTTTGGCGGCGTTAAGTTTGGCGGATAGTTGGGGTGTGCTT
GGGCAATTGTTTGGTAGCGGCTGGTCAAGCAGTCTCAATCAACAGGCTGAAATGGTGCTTGCCAGCCAGCCCGATCAGCA
ATTGGCGATTGTGTGGTTGCTGTTGATGAGCCAATTGTCCCCCAGCGCGGCCCAAACCCAAACCCAGCGGTTTAGTTTGG
CCAAGCCGATTCGCTTGATGGTTGAACAATGGCCTGAACTGGCGGCGCTTGTAGCAACGTTAAATCGAACCACGCTCGCG
GCAGGCCAACTTGATCGTTTGCTGAGTCCATTTGCGCCGTTGCTGCTTGAAGTATGGGCAAGTAATCAGGCAACATCAAT
TCAACGCCGAATTAAGTATTATTTGCAATACCTACGGCCAATTGCTAGCGCCTTGACTGGGCGTGATCTGCAAGCCCAAG
GAATTGCTGCTGGCCCACAGTATAAAACCTTGCTGGCCGAGGCTCGTGAAGGCCAACTTGATCAATTTGAACGAGAAAAC
CCGAATCTTTGCTAA

Upstream 100 bases:

>100_bases
CTTCATGCCATTGGTTAGCAAGTCGTAAGTTTTGACCAATTGTCGGTTACAATGCCCTCACTCAATGCGAGTGAGGGCAT
TTTTTTGTTGAGATCGCTCA

Downstream 100 bases:

>100_bases
GATAGTTGTGGATTATTTAAGGCTATGTATTCGCCCTTGAGCTAGGAGTAATGCCAATGACGACATTCGATTTAAGTGGC
AAGGTGGCAATCGTAACTGG

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLYDLNPFLRQASAELQQRLQWLIQTADTQGLACYLVGGCVRDWLLERPVGDIDIVVEGDAIMLAEIWATVWPDQLHIHP
PFGTATLSWQDAAGVCSLDLISARSEYYPQPAALPVVSFANLAADLARRDFTINCLALRLANQPTLLIDPQHGYADLQQS
LIRVLHDQSFIDDPTRILRAIRFAARLNFELEPQTATLLQQALPWLDQTTPARLWNELGLLLTEPSALAALSLADSWGVL
GQLFGSGWSSSLNQQAEMVLASQPDQQLAIVWLLLMSQLSPSAAQTQTQRFSLAKPIRLMVEQWPELAALVATLNRTTLA
AGQLDRLLSPFAPLLLEVWASNQATSIQRRIKYYLQYLRPIASALTGRDLQAQGIAAGPQYKTLLAEAREGQLDQFEREN
PNLC

Sequences:

>Translated_404_residues
MLYDLNPFLRQASAELQQRLQWLIQTADTQGLACYLVGGCVRDWLLERPVGDIDIVVEGDAIMLAEIWATVWPDQLHIHP
PFGTATLSWQDAAGVCSLDLISARSEYYPQPAALPVVSFANLAADLARRDFTINCLALRLANQPTLLIDPQHGYADLQQS
LIRVLHDQSFIDDPTRILRAIRFAARLNFELEPQTATLLQQALPWLDQTTPARLWNELGLLLTEPSALAALSLADSWGVL
GQLFGSGWSSSLNQQAEMVLASQPDQQLAIVWLLLMSQLSPSAAQTQTQRFSLAKPIRLMVEQWPELAALVATLNRTTLA
AGQLDRLLSPFAPLLLEVWASNQATSIQRRIKYYLQYLRPIASALTGRDLQAQGIAAGPQYKTLLAEAREGQLDQFEREN
PNLC
>Mature_404_residues
MLYDLNPFLRQASAELQQRLQWLIQTADTQGLACYLVGGCVRDWLLERPVGDIDIVVEGDAIMLAEIWATVWPDQLHIHP
PFGTATLSWQDAAGVCSLDLISARSEYYPQPAALPVVSFANLAADLARRDFTINCLALRLANQPTLLIDPQHGYADLQQS
LIRVLHDQSFIDDPTRILRAIRFAARLNFELEPQTATLLQQALPWLDQTTPARLWNELGLLLTEPSALAALSLADSWGVL
GQLFGSGWSSSLNQQAEMVLASQPDQQLAIVWLLLMSQLSPSAAQTQTQRFSLAKPIRLMVEQWPELAALVATLNRTTLA
AGQLDRLLSPFAPLLLEVWASNQATSIQRRIKYYLQYLRPIASALTGRDLQAQGIAAGPQYKTLLAEAREGQLDQFEREN
PNLC

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=225, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081691, Length=193, Percent_Identity=29.0155440414508, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=224, Percent_Identity=27.6785714285714, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 44898; Mature: 44898

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYDLNPFLRQASAELQQRLQWLIQTADTQGLACYLVGGCVRDWLLERPVGDIDIVVEGD
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECC
AIMLAEIWATVWPDQLHIHPPFGTATLSWQDAAGVCSLDLISARSEYYPQPAALPVVSFA
EEHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
NLAADLARRDFTINCLALRLANQPTLLIDPQHGYADLQQSLIRVLHDQSFIDDPTRILRA
HHHHHHHHCCCEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
IRFAARLNFELEPQTATLLQQALPWLDQTTPARLWNELGLLLTEPSALAALSLADSWGVL
HHHHHHCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
GQLFGSGWSSSLNQQAEMVLASQPDQQLAIVWLLLMSQLSPSAAQTQTQRFSLAKPIRLM
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VEQWPELAALVATLNRTTLAAGQLDRLLSPFAPLLLEVWASNQATSIQRRIKYYLQYLRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IASALTGRDLQAQGIAAGPQYKTLLAEAREGQLDQFERENPNLC
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC
>Mature Secondary Structure
MLYDLNPFLRQASAELQQRLQWLIQTADTQGLACYLVGGCVRDWLLERPVGDIDIVVEGD
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECC
AIMLAEIWATVWPDQLHIHPPFGTATLSWQDAAGVCSLDLISARSEYYPQPAALPVVSFA
EEHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
NLAADLARRDFTINCLALRLANQPTLLIDPQHGYADLQQSLIRVLHDQSFIDDPTRILRA
HHHHHHHHCCCEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
IRFAARLNFELEPQTATLLQQALPWLDQTTPARLWNELGLLLTEPSALAALSLADSWGVL
HHHHHHCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHH
GQLFGSGWSSSLNQQAEMVLASQPDQQLAIVWLLLMSQLSPSAAQTQTQRFSLAKPIRLM
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VEQWPELAALVATLNRTTLAAGQLDRLLSPFAPLLLEVWASNQATSIQRRIKYYLQYLRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IASALTGRDLQAQGIAAGPQYKTLLAEAREGQLDQFERENPNLC
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]