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The map label for this gene is pyk1 [H]

Identifier: 159899121

GI number: 159899121

Start: 3341993

End: 3343408

Strand: Reverse

Name: pyk1 [H]

Synonym: Haur_2602

Alternate gene names: 159899121

Gene position: 3343408-3341993 (Counterclockwise)

Preceding gene: 159899122

Following gene: 159899117

Centisome position: 52.68

GC content: 51.62

Gene sequence:

>1416_bases
ATGCGCCGAACCAAAATTGTTGCCACGCTCGGGCCAGCCTCTGATACTGAGGAAAAAATGACAGCGTTGGCATTAGCGGG
AATGAATGTTGCTCGCTTAAATTTCTCCCATGGTACTCATAACGACCATTCAGAACGCATTCGGCTCCTGCGTCAAATTT
CGATTAAGCTCAATCGCCCGATTGCAGTGCTGCAAGACTTGCAAGGGCCAAAAATTCGCACTGGCAAATTAGTTGATAAA
CAGCCGATTCCGCTCAAGGCCGGCGAAAGTTTCGTTATTACGATTGAAAATGAAGTTGGTCGGGTTGGCCGCGTCAACAC
CACCTATAAAAATTTACCCACCGATTGTAAGCCTGGCGACCGAATTTTGCTCTCCGATGGTTTGATCGAGTTGCGCGTAC
AAGAATGCACCAAAACTGAAGTGATCACTGAAGTGGTAACTGGCGGGATGTTGCGCGAGAATCAAGGCATCAACTTGCCT
GGCGTAGCCGTTAGTGCTCCGGCCATGACCGAAAAAGATCGTGAAGATTTGATCTTTGGCTTGCACGAAGGGGTTGATTA
TGTGGCAATCTCGTTTGTGCGCCGCGCCGAAGATGTGCAATTGGTTAAGCAAGAAATTGCTGGGGCTGGCTATCGCACCC
CAGTGATCGCCAAAATTGAAAAACCCGAAGCGGTTGATAATATCGAATCAATCGTTGCCGTCGCTGACGGGATCATGGTT
GCGCGGGGTGATTTGGGCGTAGAAATGCCGCCTGAAGAAGTGCCATTGATTCAAAAATTGATCATTGCTTCCGCCAATCG
CGCTGGAATTCCGGTGATCACTGCGACCCAAATGCTCGAATCGATGATTGTCAACCCACGGCCAACTCGCGCCGAAGCCA
GCGACGTGGCTAACGCGATTCTCGATGGCACTGATGCAGTGATGCTTAGTGGTGAAACCGCCAACGGAGCCTATCCAGTT
GAAGCGGTGACGATTATGGCGCGAATTGCCGAAGTAACTGAGCGCGGCGCACCACCACGTGATGCCCAACAACGTAACGC
GTCTGATGCCCCCGAAACCTACTCGCACGCAATCAGCCATGCTGCTTGTGCAATTGCCGAATCGATCGATATTGCGGCGA
TTGTGGCATTTACTTCATCGGGCTTTACAGCGCGGTTGGTCGCCCGCGATCGCCCGCGAGTGCCGATTATCGCCATGACC
CACGAATACGATGTCTATCATCGTTTGGCCTTGCTTTGGGGAGTTGAATCCTTGCTGTGCCCATTCGTGACGCGGCTTGA
CGATTTGAGCAACTTGGCACGGGCAATTATGATCGAGCGTCAAGTAATTAAGCCCGGCGATTCGGTCGTGATTACGGGCG
GTCACCCCTTGGCGGTGCGTGGCACAACCAACTTCCTCAAAATTCTTTCAATCTAA

Upstream 100 bases:

>100_bases
ATAATTGGAGCGAATTGAATTAGTCATCACTTGAGAACTTTAGGTCACTGTTTCGCCAAAGATGCCGACGCGGTGTTCCG
CACTTAACACGAGGTCATCA

Downstream 100 bases:

>100_bases
ACAAAAAAGCCCTAGGCCGCTCAATAACGAGCGGCCTAGGGCTTTCCATTCAGCAAATTTAACGAACCGTCAACGGTGCA
TAGACTGCATAGCTTGGCGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRRTKIVATLGPASDTEEKMTALALAGMNVARLNFSHGTHNDHSERIRLLRQISIKLNRPIAVLQDLQGPKIRTGKLVDK
QPIPLKAGESFVITIENEVGRVGRVNTTYKNLPTDCKPGDRILLSDGLIELRVQECTKTEVITEVVTGGMLRENQGINLP
GVAVSAPAMTEKDREDLIFGLHEGVDYVAISFVRRAEDVQLVKQEIAGAGYRTPVIAKIEKPEAVDNIESIVAVADGIMV
ARGDLGVEMPPEEVPLIQKLIIASANRAGIPVITATQMLESMIVNPRPTRAEASDVANAILDGTDAVMLSGETANGAYPV
EAVTIMARIAEVTERGAPPRDAQQRNASDAPETYSHAISHAACAIAESIDIAAIVAFTSSGFTARLVARDRPRVPIIAMT
HEYDVYHRLALLWGVESLLCPFVTRLDDLSNLARAIMIERQVIKPGDSVVITGGHPLAVRGTTNFLKILSI

Sequences:

>Translated_471_residues
MRRTKIVATLGPASDTEEKMTALALAGMNVARLNFSHGTHNDHSERIRLLRQISIKLNRPIAVLQDLQGPKIRTGKLVDK
QPIPLKAGESFVITIENEVGRVGRVNTTYKNLPTDCKPGDRILLSDGLIELRVQECTKTEVITEVVTGGMLRENQGINLP
GVAVSAPAMTEKDREDLIFGLHEGVDYVAISFVRRAEDVQLVKQEIAGAGYRTPVIAKIEKPEAVDNIESIVAVADGIMV
ARGDLGVEMPPEEVPLIQKLIIASANRAGIPVITATQMLESMIVNPRPTRAEASDVANAILDGTDAVMLSGETANGAYPV
EAVTIMARIAEVTERGAPPRDAQQRNASDAPETYSHAISHAACAIAESIDIAAIVAFTSSGFTARLVARDRPRVPIIAMT
HEYDVYHRLALLWGVESLLCPFVTRLDDLSNLARAIMIERQVIKPGDSVVITGGHPLAVRGTTNFLKILSI
>Mature_471_residues
MRRTKIVATLGPASDTEEKMTALALAGMNVARLNFSHGTHNDHSERIRLLRQISIKLNRPIAVLQDLQGPKIRTGKLVDK
QPIPLKAGESFVITIENEVGRVGRVNTTYKNLPTDCKPGDRILLSDGLIELRVQECTKTEVITEVVTGGMLRENQGINLP
GVAVSAPAMTEKDREDLIFGLHEGVDYVAISFVRRAEDVQLVKQEIAGAGYRTPVIAKIEKPEAVDNIESIVAVADGIMV
ARGDLGVEMPPEEVPLIQKLIIASANRAGIPVITATQMLESMIVNPRPTRAEASDVANAILDGTDAVMLSGETANGAYPV
EAVTIMARIAEVTERGAPPRDAQQRNASDAPETYSHAISHAACAIAESIDIAAIVAFTSSGFTARLVARDRPRVPIIAMT
HEYDVYHRLALLWGVESLLCPFVTRLDDLSNLARAIMIERQVIKPGDSVVITGGHPLAVRGTTNFLKILSI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=42.0408163265306, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=42.0408163265306, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=42.4489795918367, Blast_Score=335, Evalue=8e-92,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=41.3441955193483, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=41.3441955193483, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=43.9024390243902, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=43.9024390243902, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=41.2765957446808, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=41.2765957446808, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI310128738, Length=211, Percent_Identity=41.7061611374408, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=45.4935622317597, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=40.9185803757829, Blast_Score=320, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=40.7407407407407, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=43.1211498973306, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=43.1211498973306, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=43.1211498973306, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=43.1211498973306, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=37.8099173553719, Blast_Score=303, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=38.0368098159509, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24648964, Length=501, Percent_Identity=40.3193612774451, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI28571814, Length=501, Percent_Identity=40.3193612774451, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=42.997542997543, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24581235, Length=494, Percent_Identity=31.9838056680162, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24646914, Length=254, Percent_Identity=42.9133858267717, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50906; Mature: 50906

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKIVATLGPASDTEEKMTALALAGMNVARLNFSHGTHNDHSERIRLLRQISIKLNRP
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
IAVLQDLQGPKIRTGKLVDKQPIPLKAGESFVITIENEVGRVGRVNTTYKNLPTDCKPGD
HHHHHCCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCCC
RILLSDGLIELRVQECTKTEVITEVVTGGMLRENQGINLPGVAVSAPAMTEKDREDLIFG
EEEEECCEEEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCCHHHHHHH
LHEGVDYVAISFVRRAEDVQLVKQEIAGAGYRTPVIAKIEKPEAVDNIESIVAVADGIMV
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEE
ARGDLGVEMPPEEVPLIQKLIIASANRAGIPVITATQMLESMIVNPRPTRAEASDVANAI
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHH
LDGTDAVMLSGETANGAYPVEAVTIMARIAEVTERGAPPRDAQQRNASDAPETYSHAISH
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHH
AACAIAESIDIAAIVAFTSSGFTARLVARDRPRVPIIAMTHEYDVYHRLALLWGVESLLC
HHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PFVTRLDDLSNLARAIMIERQVIKPGDSVVITGGHPLAVRGTTNFLKILSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEECC
>Mature Secondary Structure
MRRTKIVATLGPASDTEEKMTALALAGMNVARLNFSHGTHNDHSERIRLLRQISIKLNRP
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
IAVLQDLQGPKIRTGKLVDKQPIPLKAGESFVITIENEVGRVGRVNTTYKNLPTDCKPGD
HHHHHCCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCCC
RILLSDGLIELRVQECTKTEVITEVVTGGMLRENQGINLPGVAVSAPAMTEKDREDLIFG
EEEEECCEEEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCCHHHHHHH
LHEGVDYVAISFVRRAEDVQLVKQEIAGAGYRTPVIAKIEKPEAVDNIESIVAVADGIMV
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEE
ARGDLGVEMPPEEVPLIQKLIIASANRAGIPVITATQMLESMIVNPRPTRAEASDVANAI
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHH
LDGTDAVMLSGETANGAYPVEAVTIMARIAEVTERGAPPRDAQQRNASDAPETYSHAISH
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHH
AACAIAESIDIAAIVAFTSSGFTARLVARDRPRVPIIAMTHEYDVYHRLALLWGVESLLC
HHHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
PFVTRLDDLSNLARAIMIERQVIKPGDSVVITGGHPLAVRGTTNFLKILSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]