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The map label for this gene is potD [C]

Identifier: 159898990

GI number: 159898990

Start: 3161287

End: 3162459

Strand: Direct

Name: potD [C]

Synonym: Haur_2471

Alternate gene names: 159898990

Gene position: 3161287-3162459 (Clockwise)

Preceding gene: 159898988

Following gene: 159898991

Centisome position: 49.81

GC content: 50.64

Gene sequence:

>1173_bases
ATGGCTCAATCGAAGCTTGGTGCTTTTCGGCTACGTGCTGCTAAATTAGCGGCCTTATTCCTCGTCGTTTCGTTGATGAT
TAGTGCGTGTGGCTCAGCTGCCAGCGAAAGCACCACCGCCCCAACCGCTGGCTCAGGTGGCACATCATCAACTATGGATG
CTTTGATTGCTGCTGCCAAAGCCGAAGGCGAATTAACCGTGATCGCCTTGCCCCACAATTGGCTCAACTACGGCGAAATG
ATCGAAAACTTCTCGAAAAAATATGGCATCAAAATCAACGAACTGAATCCTGATGCTGGTTCGGGCGACGAAATCGAAGC
GATCAAGGCCAATAAAGATAACAAAGGCCCACAAGCCCCCGATGTGATCGACGTTGGCTTTGCTTTTGGTCCATCCGCCA
AGCAAGAAAATCTCTTGCAGCCCTATAAAGTTTCAACCTGGGATACGATTCCTAACGAGTTGAAAGATCCTGAGGGCTAT
TGGTTTGGCGATTACTATGGCGTGTTGGCGTTTGCCGTCAACAAAGATGTGGTCAAAAATGTGCCGCAGGATTGGGCCGA
CCTCTTGAAGCCTGAATACAAAGGCCAAGTCGCCTTGGCAGGCGATCCACGGGTCTCAAACTTGGCGATTCAGTCAGTCT
ACGCCGCAGCACTTGCTAATGGTGGTAGCTTGGATAACGTTCAACCTGGCTTGGATTTCTTCAGCAAATTGAACCAAGCT
GGCAACTTTGTGCCAGTTATCGCCAAACAAGGTACCTTGGCCCAGGGCGAAACCCCAATTATGATCACCTGGGACTATTT
GGCAATCAGCGCTCGCGATGAATTGGGCGGTAACCCTGAAATCGAAGTGGTTGTGCCAAAATCAGGCGTGCTTGGTGGCG
TGTATGTTCAAGCGATCAGCGCTTATGCTCCGCACCCAAATGCTGCCAAATTGTGGATGGAATACCTCTACTCCGACGAA
GGCCAATTGACTTGGCTCAAAGGCGGCGGCCACCCAGTGCGCTACAACGATTTGGTTGCTCGCAATGTAATTCCAGCCGA
AATTGCGGAAAAATTGCCACCAGCCGAACTCTACGCCAACGCCGTATTCCCAACCTTGGCGCAGCTCGAAGCTGCCAAAA
AAGTTATCGTCGATGGTTGGGATACCACGGTCAACGTCGATGTCAAAGAATAA

Upstream 100 bases:

>100_bases
CCCCCGTAGCATCAACCCAGCCCATTTTTTGTGGTTACACTCGTCAATAACCCGACAGATTCTCTTGCATCGTGGTCTAG
CTTGCTAGGAGATTTAACCC

Downstream 100 bases:

>100_bases
TTAAGGAAGGATCGCCGCTCTGTGGCAGAACAAGCTTCAGAACGTGGTCGGGGTGCGCAATGGCAGCACCTCGACCTTCG
TCGCATTAATTGGAGTTGGC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MAQSKLGAFRLRAAKLAALFLVVSLMISACGSAASESTTAPTAGSGGTSSTMDALIAAAKAEGELTVIALPHNWLNYGEM
IENFSKKYGIKINELNPDAGSGDEIEAIKANKDNKGPQAPDVIDVGFAFGPSAKQENLLQPYKVSTWDTIPNELKDPEGY
WFGDYYGVLAFAVNKDVVKNVPQDWADLLKPEYKGQVALAGDPRVSNLAIQSVYAAALANGGSLDNVQPGLDFFSKLNQA
GNFVPVIAKQGTLAQGETPIMITWDYLAISARDELGGNPEIEVVVPKSGVLGGVYVQAISAYAPHPNAAKLWMEYLYSDE
GQLTWLKGGGHPVRYNDLVARNVIPAEIAEKLPPAELYANAVFPTLAQLEAAKKVIVDGWDTTVNVDVKE

Sequences:

>Translated_390_residues
MAQSKLGAFRLRAAKLAALFLVVSLMISACGSAASESTTAPTAGSGGTSSTMDALIAAAKAEGELTVIALPHNWLNYGEM
IENFSKKYGIKINELNPDAGSGDEIEAIKANKDNKGPQAPDVIDVGFAFGPSAKQENLLQPYKVSTWDTIPNELKDPEGY
WFGDYYGVLAFAVNKDVVKNVPQDWADLLKPEYKGQVALAGDPRVSNLAIQSVYAAALANGGSLDNVQPGLDFFSKLNQA
GNFVPVIAKQGTLAQGETPIMITWDYLAISARDELGGNPEIEVVVPKSGVLGGVYVQAISAYAPHPNAAKLWMEYLYSDE
GQLTWLKGGGHPVRYNDLVARNVIPAEIAEKLPPAELYANAVFPTLAQLEAAKKVIVDGWDTTVNVDVKE
>Mature_389_residues
AQSKLGAFRLRAAKLAALFLVVSLMISACGSAASESTTAPTAGSGGTSSTMDALIAAAKAEGELTVIALPHNWLNYGEMI
ENFSKKYGIKINELNPDAGSGDEIEAIKANKDNKGPQAPDVIDVGFAFGPSAKQENLLQPYKVSTWDTIPNELKDPEGYW
FGDYYGVLAFAVNKDVVKNVPQDWADLLKPEYKGQVALAGDPRVSNLAIQSVYAAALANGGSLDNVQPGLDFFSKLNQAG
NFVPVIAKQGTLAQGETPIMITWDYLAISARDELGGNPEIEVVVPKSGVLGGVYVQAISAYAPHPNAAKLWMEYLYSDEG
QLTWLKGGGHPVRYNDLVARNVIPAEIAEKLPPAELYANAVFPTLAQLEAAKKVIVDGWDTTVNVDVKE

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41645; Mature: 41513

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSKLGAFRLRAAKLAALFLVVSLMISACGSAASESTTAPTAGSGGTSSTMDALIAAAK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
AEGELTVIALPHNWLNYGEMIENFSKKYGIKINELNPDAGSGDEIEAIKANKDNKGPQAP
CCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
DVIDVGFAFGPSAKQENLLQPYKVSTWDTIPNELKDPEGYWFGDYYGVLAFAVNKDVVKN
CEEEECCCCCCCCCHHCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHCHHHHHC
VPQDWADLLKPEYKGQVALAGDPRVSNLAIQSVYAAALANGGSLDNVQPGLDFFSKLNQA
CCHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
GNFVPVIAKQGTLAQGETPIMITWDYLAISARDELGGNPEIEVVVPKSGVLGGVYVQAIS
CCEEEEEECCCCCCCCCCCEEEEEEEEEEEEHHHCCCCCCEEEEECCCCCCHHHHHHHHH
AYAPHPNAAKLWMEYLYSDEGQLTWLKGGGHPVRYNDLVARNVIPAEIAEKLPPAELYAN
HCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHCCCHHHHHHHCCHHHHHHH
AVFPTLAQLEAAKKVIVDGWDTTVNVDVKE
HHHHHHHHHHHHHHEEEECCCCEEEEEECC
>Mature Secondary Structure 
AQSKLGAFRLRAAKLAALFLVVSLMISACGSAASESTTAPTAGSGGTSSTMDALIAAAK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
AEGELTVIALPHNWLNYGEMIENFSKKYGIKINELNPDAGSGDEIEAIKANKDNKGPQAP
CCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
DVIDVGFAFGPSAKQENLLQPYKVSTWDTIPNELKDPEGYWFGDYYGVLAFAVNKDVVKN
CEEEECCCCCCCCCHHCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHCHHHHHC
VPQDWADLLKPEYKGQVALAGDPRVSNLAIQSVYAAALANGGSLDNVQPGLDFFSKLNQA
CCHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
GNFVPVIAKQGTLAQGETPIMITWDYLAISARDELGGNPEIEVVVPKSGVLGGVYVQAIS
CCEEEEEECCCCCCCCCCCEEEEEEEEEEEEHHHCCCCCCEEEEECCCCCCHHHHHHHHH
AYAPHPNAAKLWMEYLYSDEGQLTWLKGGGHPVRYNDLVARNVIPAEIAEKLPPAELYAN
HCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHCCCHHHHHHHCCHHHHHHH
AVFPTLAQLEAAKKVIVDGWDTTVNVDVKE
HHHHHHHHHHHHHHEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]