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The map label for this gene is cobB [H]

Identifier: 159898977

GI number: 159898977

Start: 3141214

End: 3141729

Strand: Reverse

Name: cobB [H]

Synonym: Haur_2458

Alternate gene names: 159898977

Gene position: 3141729-3141214 (Counterclockwise)

Preceding gene: 159898978

Following gene: 159898976

Centisome position: 49.5

GC content: 55.04

Gene sequence:

>516_bases
ATGCTTGATGTTCAGCCGAATGCTGGCCACGTTGCTTTAGCCCAACTTGAGCAGCATATCCCAAGTGTGACGATTATTAC
CCAAAATATTGATGGACTGCATCAGCGGGCTGGCTCAACGAGGGTGATCGAGTTGCATGGCACGATTAATACGGTGAGTT
GTAGCGCCGCTGAACATGGCAGTTTGGCTTGGCCAGATAGCCCAAATTTGCCGTTTTGTTCAGTTTGTGCTGCACCGCTG
CGTCCCGATGTGGTCTGGTTTGGTGAACGTTTGGATCTTGCCAAGATTCAGGCGGCTGAATTAGCCAGCCAAACCTGTGA
TGTATTTCTGGCGATCGGCACATCGGGCGTGGTCGCGCCAGCCGCGACCTTTCCGATGACTGCCCGTGCCCATCGTGCCC
GTTTGATCGATCTCAACCTTGAGGATACGCCGTTGAGTCGCCATGCTCGCCATCGTTTACGCGGCACGGCGGCGCAGCTA
TTGCCAGCCTTGCTGGCTGCGACTTGGGCTGAGTAA

Upstream 100 bases:

>100_bases
GCTGTGAAATACGATTGATGTTGAGCAAGTTGCAACTTTAGCAGGCTATTTGCAGCAACCTGAGGCCGTTTGGGCTTGGT
ATCAGGCGTATCGTCAGCAG

Downstream 100 bases:

>100_bases
TGATTCAATCTTGAAGGCTTGATTATAAGCTTGTATACTGTGCATGCCTAACAACCCTTGAAATTGAATTGTGAGTTTGA
CTGATGAATCAATCCCAGAT

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MLDVQPNAGHVALAQLEQHIPSVTIITQNIDGLHQRAGSTRVIELHGTINTVSCSAAEHGSLAWPDSPNLPFCSVCAAPL
RPDVVWFGERLDLAKIQAAELASQTCDVFLAIGTSGVVAPAATFPMTARAHRARLIDLNLEDTPLSRHARHRLRGTAAQL
LPALLAATWAE

Sequences:

>Translated_171_residues
MLDVQPNAGHVALAQLEQHIPSVTIITQNIDGLHQRAGSTRVIELHGTINTVSCSAAEHGSLAWPDSPNLPFCSVCAAPL
RPDVVWFGERLDLAKIQAAELASQTCDVFLAIGTSGVVAPAATFPMTARAHRARLIDLNLEDTPLSRHARHRLRGTAAQL
LPALLAATWAE
>Mature_171_residues
MLDVQPNAGHVALAQLEQHIPSVTIITQNIDGLHQRAGSTRVIELHGTINTVSCSAAEHGSLAWPDSPNLPFCSVCAAPL
RPDVVWFGERLDLAKIQAAELASQTCDVFLAIGTSGVVAPAATFPMTARAHRARLIDLNLEDTPLSRHARHRLRGTAAQL
LPALLAATWAE

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=180, Percent_Identity=35.5555555555556, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI6912664, Length=194, Percent_Identity=34.5360824742268, Blast_Score=101, Evalue=4e-22,
Organism=Homo sapiens, GI13787215, Length=179, Percent_Identity=34.6368715083799, Blast_Score=94, Evalue=6e-20,
Organism=Homo sapiens, GI6912662, Length=194, Percent_Identity=30.4123711340206, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI300797577, Length=179, Percent_Identity=27.3743016759777, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI63054862, Length=157, Percent_Identity=29.9363057324841, Blast_Score=64, Evalue=5e-11,
Organism=Homo sapiens, GI6912660, Length=157, Percent_Identity=29.9363057324841, Blast_Score=64, Evalue=7e-11,
Organism=Escherichia coli, GI308199517, Length=168, Percent_Identity=41.6666666666667, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571445, Length=117, Percent_Identity=32.4786324786325, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571443, Length=117, Percent_Identity=32.4786324786325, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571441, Length=117, Percent_Identity=32.4786324786325, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 18274; Mature: 18274

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDVQPNAGHVALAQLEQHIPSVTIITQNIDGLHQRAGSTRVIELHGTINTVSCSAAEHG
CCCCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHCCCCCEEEEECCCEEEEEECCCCCC
SLAWPDSPNLPFCSVCAAPLRPDVVWFGERLDLAKIQAAELASQTCDVFLAIGTSGVVAP
CEECCCCCCCCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
AATFPMTARAHRARLIDLNLEDTPLSRHARHRLRGTAAQLLPALLAATWAE
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDVQPNAGHVALAQLEQHIPSVTIITQNIDGLHQRAGSTRVIELHGTINTVSCSAAEHG
CCCCCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHCCCCCEEEEECCCEEEEEECCCCCC
SLAWPDSPNLPFCSVCAAPLRPDVVWFGERLDLAKIQAAELASQTCDVFLAIGTSGVVAP
CEECCCCCCCCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCC
AATFPMTARAHRARLIDLNLEDTPLSRHARHRLRGTAAQLLPALLAATWAE
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]