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The map label for this gene is thiM

Identifier: 159898965

GI number: 159898965

Start: 3128265

End: 3129077

Strand: Reverse

Name: thiM

Synonym: Haur_2444

Alternate gene names: 159898965

Gene position: 3129077-3128265 (Counterclockwise)

Preceding gene: 159898966

Following gene: 159898964

Centisome position: 49.3

GC content: 53.63

Gene sequence:

>813_bases
TTGTTGACACCAAGCAGCATTGCCGATTGTTGGCATTCATTACGCCAGCAACGCCCGTTGGTGCATGTTGTGCCAAATTT
GGTGACGGCCAATGATCTGGCCAATGCCTTGCTGGCGGTTGGTGCTGCGCCAATTATGGCGATCGAGCCAGCCGAATTTA
GCCAGTTGCCCAATCGCGCCCTTGTTTTGAGCATGGGCACGCCAACGATTGATCGAATGCAACTACTTGCCCAAGCCGGA
CGAGCTGCCCAAGCCAAAAATCTGCCAATTGTGCTTGATCCGGTTGGAGTTGGTGCGACCGTTTGGCGCAAACAAGCGGC
CTTGGCATTAATCGCTACGGTGCAGCCAACGATTCTGCGCTTGAATTTGGGCGAAGCCTTGGCTTTGCTCGATCAAACGG
GTGTGGCCCATGGCGTTGATGTCGGTCACGCTTGGCATGATCCAAGGCTGGTGGCAGGCCAATTGGCACGGCGCTATGGC
TGTGTAGTTGGCCTAACGGGCGTAATCGATGTGGTTAGTGATGGTATGAACTGGATTCAGCTTGAGCATGGGCATCAATG
GCTGAGCCAAATTACTGGCGCTGGTTGCATTGTAACCAGCCTGATTGGCGCACTTGCTGCGGTTATCAATGATGTTATGT
TGGCGACTGTCAGCGCAATCGCTGGCTTCGGAATGGCGGCTGAAGTTGCGGCTATGCATGCGCTTGGCCCAGCCAGTTTT
CGGGTGGCCCTGTTTGATCAGCTTGGGGCAATTGCTGAATTAATTGATAACAGTCGGCTTAGCTATCGTATGGAGCAGCA
TGATGCAGATTGA

Upstream 100 bases:

>100_bases
GCGACGATTCCAAGCCTTGTGAGCGCTGGATTGATCTATTGGGGGTTAGGCAACATTGGTTTGTTTACCAAGCAATCGGC
CTTGCAACGTGCGGCCTAAA

Downstream 100 bases:

>100_bases
TTGGCGTTTGTATGCGGTGTTGGATACCGCCACGCTTGGCTCGCGCGACCCTTTGGCCATGACGGCGGCGCTGCTGGCTG
GCGGGATTGGGGTATTACAA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLTPSSIADCWHSLRQQRPLVHVVPNLVTANDLANALLAVGAAPIMAIEPAEFSQLPNRALVLSMGTPTIDRMQLLAQAG
RAAQAKNLPIVLDPVGVGATVWRKQAALALIATVQPTILRLNLGEALALLDQTGVAHGVDVGHAWHDPRLVAGQLARRYG
CVVGLTGVIDVVSDGMNWIQLEHGHQWLSQITGAGCIVTSLIGALAAVINDVMLATVSAIAGFGMAAEVAAMHALGPASF
RVALFDQLGAIAELIDNSRLSYRMEQHDAD

Sequences:

>Translated_270_residues
MLTPSSIADCWHSLRQQRPLVHVVPNLVTANDLANALLAVGAAPIMAIEPAEFSQLPNRALVLSMGTPTIDRMQLLAQAG
RAAQAKNLPIVLDPVGVGATVWRKQAALALIATVQPTILRLNLGEALALLDQTGVAHGVDVGHAWHDPRLVAGQLARRYG
CVVGLTGVIDVVSDGMNWIQLEHGHQWLSQITGAGCIVTSLIGALAAVINDVMLATVSAIAGFGMAAEVAAMHALGPASF
RVALFDQLGAIAELIDNSRLSYRMEQHDAD
>Mature_270_residues
MLTPSSIADCWHSLRQQRPLVHVVPNLVTANDLANALLAVGAAPIMAIEPAEFSQLPNRALVLSMGTPTIDRMQLLAQAG
RAAQAKNLPIVLDPVGVGATVWRKQAALALIATVQPTILRLNLGEALALLDQTGVAHGVDVGHAWHDPRLVAGQLARRYG
CVVGLTGVIDVVSDGMNWIQLEHGHQWLSQITGAGCIVTSLIGALAAVINDVMLATVSAIAGFGMAAEVAAMHALGPASF
RVALFDQLGAIAELIDNSRLSYRMEQHDAD

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=250, Percent_Identity=33.6, Blast_Score=116, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325042, Length=277, Percent_Identity=29.6028880866426, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_HERA2 (A9AZE0)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001545212.1
- ProteinModelPortal:   A9AZE0
- SMR:   A9AZE0
- GeneID:   5734325
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_2444
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- BioCyc:   HAUR316274:HAUR_2444-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28423; Mature: 28423

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: BINDING 46-46 BINDING 120-120 BINDING 166-166 BINDING 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPSSIADCWHSLRQQRPLVHVVPNLVTANDLANALLAVGAAPIMAIEPAEFSQLPNRA
CCCCHHHHHHHHHHHHCCCEEEHHCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCE
LVLSMGTPTIDRMQLLAQAGRAAQAKNLPIVLDPVGVGATVWRKQAALALIATVQPTILR
EEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
LNLGEALALLDQTGVAHGVDVGHAWHDPRLVAGQLARRYGCVVGLTGVIDVVSDGMNWIQ
EEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LEHGHQWLSQITGAGCIVTSLIGALAAVINDVMLATVSAIAGFGMAAEVAAMHALGPASF
EHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
RVALFDQLGAIAELIDNSRLSYRMEQHDAD
HHHHHHHHHHHHHHHCCCHHHEEHHCCCCC
>Mature Secondary Structure
MLTPSSIADCWHSLRQQRPLVHVVPNLVTANDLANALLAVGAAPIMAIEPAEFSQLPNRA
CCCCHHHHHHHHHHHHCCCEEEHHCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCE
LVLSMGTPTIDRMQLLAQAGRAAQAKNLPIVLDPVGVGATVWRKQAALALIATVQPTILR
EEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
LNLGEALALLDQTGVAHGVDVGHAWHDPRLVAGQLARRYGCVVGLTGVIDVVSDGMNWIQ
EEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LEHGHQWLSQITGAGCIVTSLIGALAAVINDVMLATVSAIAGFGMAAEVAAMHALGPASF
EHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
RVALFDQLGAIAELIDNSRLSYRMEQHDAD
HHHHHHHHHHHHHHHCCCHHHEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA