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The map label for this gene is thiE

Identifier: 159898964

GI number: 159898964

Start: 3127658

End: 3128278

Strand: Reverse

Name: thiE

Synonym: Haur_2443

Alternate gene names: 159898964

Gene position: 3128278-3127658 (Counterclockwise)

Preceding gene: 159898965

Following gene: 159898963

Centisome position: 49.29

GC content: 54.59

Gene sequence:

>621_bases
ATGATGCAGATTGATTGGCGTTTGTATGCGGTGTTGGATACCGCCACGCTTGGCTCGCGCGACCCTTTGGCCATGACGGC
GGCGCTGCTGGCTGGCGGGATTGGGGTATTACAACTTCGTGCCAAAAATCTGTCCGTGCGGCAAACCGCTCAGCTGGCCC
AAGCGATCCTGCCACTCACCAAGATTGCCCAAATTCCCTTGATTATCAATGACGATTTAGGCTTAGCCTTGGCGGTTGGG
GCTGATGGCGTGCATTTGGGCGTTGATGATTTGCCCTTGGATTTGGCTCGCGCCAGTTTCAAGGGCTTGATTGGCTACTC
GCCTGAGGGAGTGGCCGATGCCCAACGGGCTGAAAAATTGGGCGTTGATTATCTTGGGGTTGGGCCATTTGCTGCCACCA
CTACCAAACTTGATGCTGGTGCGCCCTTAGGTCAGGCAGGCTTGCGAGCAATTGTTGAGGCGGTGGATTGCCCAGTTGTG
GCGATTGGCGGAATTGCTCAACATAACGTTGCAGAAGTGCGAGCTTGCGGCGTGGCTGGCATTGTGGTGGTTTCGGCCTT
GTTGAATGCGACCAATCCAACCCAAGCTTGTCGTGAATTTCTCGCACATTATAAGGAATGA

Upstream 100 bases:

>100_bases
GCGCTTGGCCCAGCCAGTTTTCGGGTGGCCCTGTTTGATCAGCTTGGGGCAATTGCTGAATTAATTGATAACAGTCGGCT
TAGCTATCGTATGGAGCAGC

Downstream 100 bases:

>100_bases
TAGATGACCAGTTTAATGCAAAAACAGCGTCAATCAACGCGACGCTTGGCCTATAGCATTGTTTTGGCAGCCTTGGGCTT
AGCACTCTCGCCCATTAGCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MMQIDWRLYAVLDTATLGSRDPLAMTAALLAGGIGVLQLRAKNLSVRQTAQLAQAILPLTKIAQIPLIINDDLGLALAVG
ADGVHLGVDDLPLDLARASFKGLIGYSPEGVADAQRAEKLGVDYLGVGPFAATTTKLDAGAPLGQAGLRAIVEAVDCPVV
AIGGIAQHNVAEVRACGVAGIVVVSALLNATNPTQACREFLAHYKE

Sequences:

>Translated_206_residues
MMQIDWRLYAVLDTATLGSRDPLAMTAALLAGGIGVLQLRAKNLSVRQTAQLAQAILPLTKIAQIPLIINDDLGLALAVG
ADGVHLGVDDLPLDLARASFKGLIGYSPEGVADAQRAEKLGVDYLGVGPFAATTTKLDAGAPLGQAGLRAIVEAVDCPVV
AIGGIAQHNVAEVRACGVAGIVVVSALLNATNPTQACREFLAHYKE
>Mature_206_residues
MMQIDWRLYAVLDTATLGSRDPLAMTAALLAGGIGVLQLRAKNLSVRQTAQLAQAILPLTKIAQIPLIINDDLGLALAVG
ADGVHLGVDDLPLDLARASFKGLIGYSPEGVADAQRAEKLGVDYLGVGPFAATTTKLDAGAPLGQAGLRAIVEAVDCPVV
AIGGIAQHNVAEVRACGVAGIVVVSALLNATNPTQACREFLAHYKE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=197, Percent_Identity=33.502538071066, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=219, Percent_Identity=30.5936073059361, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_HERA2 (A9AZD9)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001545211.1
- ProteinModelPortal:   A9AZD9
- SMR:   A9AZD9
- GeneID:   5734324
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_2443
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- BioCyc:   HAUR316274:HAUR_2443-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21240; Mature: 21240

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQIDWRLYAVLDTATLGSRDPLAMTAALLAGGIGVLQLRAKNLSVRQTAQLAQAILPLT
CCEECEEEEEEEEHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
KIAQIPLIINDDLGLALAVGADGVHLGVDDLPLDLARASFKGLIGYSPEGVADAQRAEKL
HHHHCCEEEECCCCEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GVDYLGVGPFAATTTKLDAGAPLGQAGLRAIVEAVDCPVVAIGGIAQHNVAEVRACGVAG
CCCEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCHHH
IVVVSALLNATNPTQACREFLAHYKE
HHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMQIDWRLYAVLDTATLGSRDPLAMTAALLAGGIGVLQLRAKNLSVRQTAQLAQAILPLT
CCEECEEEEEEEEHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
KIAQIPLIINDDLGLALAVGADGVHLGVDDLPLDLARASFKGLIGYSPEGVADAQRAEKL
HHHHCCEEEECCCCEEEEECCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GVDYLGVGPFAATTTKLDAGAPLGQAGLRAIVEAVDCPVVAIGGIAQHNVAEVRACGVAG
CCCEECCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHCCHHH
IVVVSALLNATNPTQACREFLAHYKE
HHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA