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The map label for this gene is tvaII [H]

Identifier: 159898956

GI number: 159898956

Start: 3118970

End: 3120325

Strand: Direct

Name: tvaII [H]

Synonym: Haur_2435

Alternate gene names: 159898956

Gene position: 3118970-3120325 (Clockwise)

Preceding gene: 159898955

Following gene: 159898957

Centisome position: 49.14

GC content: 49.26

Gene sequence:

>1356_bases
ATGCATCCATGGGCCAACGACGTACAATGTTATCAAATTTATCCCTTAGGGCTTTGCGGCGCACCAATTCGCAATGATCA
AACCAGCGAACCGATTGCGCGGCTCAAGCAATTGCACACATGGATTGAGCACTTACAACATTTAGGCAGCAACTTGCTCT
ACCTTGGGCCAGTGTTTGAATCGACAGCTCATGGCTACGACACAATTGATTATTTCACGGTTGATCGGCGGCTAGGTAGC
AACAACGATTTACAACAGTTGATTGCAGCATTCCACGCGGCGGGCATTCGTGTGTTACTCGATGGCGTGTTTAACCATGT
TGGCCGCGATTTTTGGGCCTTCCGCGATGTACAAAGCCATGGGCAGGCTTCGAGCTATAGCGATTGGTTTGCGGGCTTGG
ATTTCAATCAACACAGCCCATATGGCGATCAGTTTAGCTATCAAGGTTGGCATGGGCACTACGATTTAGTCAAATTGAAT
TTACACAATCCGGCAGTGCGCGAACATCTTTTCCAAGCAGTCAGCCAATGGATTGCACAATTTGGCATCGATGGCTTACG
GCTTGATGCTGCGGATCAGATCGATCATGATTTTTTGGCGGCGCTGGCAGCTCATTGCAAAAGCCTGCGCAGTGATTTTC
TATTGATTGGCGAAGTGGTGCATGGCGATTATCGCCAATGGGCCAACCCAACGATGCTCGATAGCGTGACCAACTATGAA
GCCTACAAAGGCCTGTATTCCAGCCTGAATGATCGCAACTATTTTGAAATTGCTTACAGTCTCAATCGTCAATTTGGCGC
TGGTGGTATCTATCGGGCTATGCCGTTGTATAACTTTGTTGATAATCATGATGTCGATCGAATCGCCAGCACCTTGCACA
ATCCAGCCCATCTCTACCCTTTACATCTATTGCTTTACACCATGCCAGGAATGCCTGCGCTGTACTATGGCAGCGAATGG
GGCTTGCTCGGCCAGAAAACCGCCACCAGCGACCAAGCCTTGCGACCAGCCTTGCCTCAACCCGACCAAATTCAGGCCCA
ACAACCAGATTTACTGGCAGCGATTCGCCAATTGAGCCAGCTACGCCACGAATATGCTGCCTTACGTTATGGCGAATATG
CCCAAATATATGTACAGGCTGAACAGCTTGTGTTTGTGCGTTGGACTAAGCAGCAAACAATTGTGGTCGCATTGAATGCC
GCCCCAACCGCCCAAACAATTCGATTTGTTGTGCCATTTGGTGAAGGCTCACTGCTTGAAGATCGCTTGAATGGCGGGGA
AATCAAGGTGCAGCATGGCCAATTACAGCTGACAATTCCCGCAACCTGGGGCCAAATCTGGCAGCTGATTGATTGA

Upstream 100 bases:

>100_bases
CCGCACATCATTCACCAAGCAGTTCAGCAATTTTCATATGGCATTTATTCCTAAATTTTTTTAGCCATCACACTATACCG
ATTGGTATACAGGAGAAGTT

Downstream 100 bases:

>100_bases
GTGTGAAGCCTAAACCTAATCACGGAATCCGAACGTCTCGAAAACTATCTTTATACGATACCCTTCCTCGGGGCGTTGAT
TGAAATTTTCTCGTGCATTT

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: Alpha-amylase II; TVA II [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MHPWANDVQCYQIYPLGLCGAPIRNDQTSEPIARLKQLHTWIEHLQHLGSNLLYLGPVFESTAHGYDTIDYFTVDRRLGS
NNDLQQLIAAFHAAGIRVLLDGVFNHVGRDFWAFRDVQSHGQASSYSDWFAGLDFNQHSPYGDQFSYQGWHGHYDLVKLN
LHNPAVREHLFQAVSQWIAQFGIDGLRLDAADQIDHDFLAALAAHCKSLRSDFLLIGEVVHGDYRQWANPTMLDSVTNYE
AYKGLYSSLNDRNYFEIAYSLNRQFGAGGIYRAMPLYNFVDNHDVDRIASTLHNPAHLYPLHLLLYTMPGMPALYYGSEW
GLLGQKTATSDQALRPALPQPDQIQAQQPDLLAAIRQLSQLRHEYAALRYGEYAQIYVQAEQLVFVRWTKQQTIVVALNA
APTAQTIRFVVPFGEGSLLEDRLNGGEIKVQHGQLQLTIPATWGQIWQLID

Sequences:

>Translated_451_residues
MHPWANDVQCYQIYPLGLCGAPIRNDQTSEPIARLKQLHTWIEHLQHLGSNLLYLGPVFESTAHGYDTIDYFTVDRRLGS
NNDLQQLIAAFHAAGIRVLLDGVFNHVGRDFWAFRDVQSHGQASSYSDWFAGLDFNQHSPYGDQFSYQGWHGHYDLVKLN
LHNPAVREHLFQAVSQWIAQFGIDGLRLDAADQIDHDFLAALAAHCKSLRSDFLLIGEVVHGDYRQWANPTMLDSVTNYE
AYKGLYSSLNDRNYFEIAYSLNRQFGAGGIYRAMPLYNFVDNHDVDRIASTLHNPAHLYPLHLLLYTMPGMPALYYGSEW
GLLGQKTATSDQALRPALPQPDQIQAQQPDLLAAIRQLSQLRHEYAALRYGEYAQIYVQAEQLVFVRWTKQQTIVVALNA
APTAQTIRFVVPFGEGSLLEDRLNGGEIKVQHGQLQLTIPATWGQIWQLID
>Mature_451_residues
MHPWANDVQCYQIYPLGLCGAPIRNDQTSEPIARLKQLHTWIEHLQHLGSNLLYLGPVFESTAHGYDTIDYFTVDRRLGS
NNDLQQLIAAFHAAGIRVLLDGVFNHVGRDFWAFRDVQSHGQASSYSDWFAGLDFNQHSPYGDQFSYQGWHGHYDLVKLN
LHNPAVREHLFQAVSQWIAQFGIDGLRLDAADQIDHDFLAALAAHCKSLRSDFLLIGEVVHGDYRQWANPTMLDSVTNYE
AYKGLYSSLNDRNYFEIAYSLNRQFGAGGIYRAMPLYNFVDNHDVDRIASTLHNPAHLYPLHLLLYTMPGMPALYYGSEW
GLLGQKTATSDQALRPALPQPDQIQAQQPDLLAAIRQLSQLRHEYAALRYGEYAQIYVQAEQLVFVRWTKQQTIVVALNA
APTAQTIRFVVPFGEGSLLEDRLNGGEIKVQHGQLQLTIPATWGQIWQLID

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=444, Percent_Identity=27.9279279279279, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1790687, Length=373, Percent_Identity=25.4691689008043, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322241, Length=211, Percent_Identity=21.3270142180095, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=211, Percent_Identity=21.3270142180095, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324416, Length=211, Percent_Identity=21.3270142180095, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24586591, Length=208, Percent_Identity=25.4807692307692, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24586587, Length=214, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583747, Length=398, Percent_Identity=23.6180904522613, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583749, Length=391, Percent_Identity=23.7851662404092, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45549022, Length=209, Percent_Identity=25.8373205741627, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24586599, Length=388, Percent_Identity=21.9072164948454, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 51178; Mature: 51178

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPWANDVQCYQIYPLGLCGAPIRNDQTSEPIARLKQLHTWIEHLQHLGSNLLYLGPVFE
CCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHH
STAHGYDTIDYFTVDRRLGSNNDLQQLIAAFHAAGIRVLLDGVFNHVGRDFWAFRDVQSH
HCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GQASSYSDWFAGLDFNQHSPYGDQFSYQGWHGHYDLVKLNLHNPAVREHLFQAVSQWIAQ
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
FGIDGLRLDAADQIDHDFLAALAAHCKSLRSDFLLIGEVVHGDYRQWANPTMLDSVTNYE
HCCCCEEECCHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHHCCHHHCCCCHHHHHHHHHH
AYKGLYSSLNDRNYFEIAYSLNRQFGAGGIYRAMPLYNFVDNHDVDRIASTLHNPAHLYP
HHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
LHLLLYTMPGMPALYYGSEWGLLGQKTATSDQALRPALPQPDQIQAQQPDLLAAIRQLSQ
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LRHEYAALRYGEYAQIYVQAEQLVFVRWTKQQTIVVALNAAPTAQTIRFVVPFGEGSLLE
HHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCHH
DRLNGGEIKVQHGQLQLTIPATWGQIWQLID
HCCCCCEEEEEECEEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MHPWANDVQCYQIYPLGLCGAPIRNDQTSEPIARLKQLHTWIEHLQHLGSNLLYLGPVFE
CCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHH
STAHGYDTIDYFTVDRRLGSNNDLQQLIAAFHAAGIRVLLDGVFNHVGRDFWAFRDVQSH
HCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
GQASSYSDWFAGLDFNQHSPYGDQFSYQGWHGHYDLVKLNLHNPAVREHLFQAVSQWIAQ
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
FGIDGLRLDAADQIDHDFLAALAAHCKSLRSDFLLIGEVVHGDYRQWANPTMLDSVTNYE
HCCCCEEECCHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHHCCHHHCCCCHHHHHHHHHH
AYKGLYSSLNDRNYFEIAYSLNRQFGAGGIYRAMPLYNFVDNHDVDRIASTLHNPAHLYP
HHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHH
LHLLLYTMPGMPALYYGSEWGLLGQKTATSDQALRPALPQPDQIQAQQPDLLAAIRQLSQ
HHHHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LRHEYAALRYGEYAQIYVQAEQLVFVRWTKQQTIVVALNAAPTAQTIRFVVPFGEGSLLE
HHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCHH
DRLNGGEIKVQHGQLQLTIPATWGQIWQLID
HCCCCCEEEEEECEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7763540; 10222200; 12051850 [H]