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The map label for this gene is nadB [H]

Identifier: 159898830

GI number: 159898830

Start: 2948969

End: 2950492

Strand: Reverse

Name: nadB [H]

Synonym: Haur_2309

Alternate gene names: 159898830

Gene position: 2950492-2948969 (Counterclockwise)

Preceding gene: 159898831

Following gene: 159898829

Centisome position: 46.49

GC content: 55.51

Gene sequence:

>1524_bases
ATGAACGATTATATTATTGTTGGCTCAGGCATCGCTGGTTTATACACTGCCCAACTTGCTGCTCAACATAATCTCAAAAT
TGTGTTGGTGACCAAAGAGGCAGTTGAGGAATGTAACACGCGCTATGCCCAAGGTGGAATTGCCGCCGCGATTGGTGAGC
ACGATTCGCCCGAATTGCACATTGAAGATACTTTGGTCGCTGGCGCTGGCATCAGCGATCCAGCAGCCGTGCGCGTTTTG
TGCGAAGAAGGCCCAGCGCGGGTGCACGAGTTGATCAATTTGGGTGTGCCGTTTGATACCCACGATGGTGCAATCGCTCT
GACTCGTGAAGCCGCCCACTCCGAACGCCGCATTTTGCACGCTGGTGGTGATGCAACAGGCGCAGCGATCGAAACAACCT
TGGCAGGCGTGGTTGCGAATACCAATGTTGATGTGCGGGCTTGGACTTTAGCCACCGAAATTATGCTAGAAAACGGCCAA
GCGGTTGGTGTGCGAACGCTTGATGCGCGGACTGGCGAGCGCGGCGAGGTTCGCGCCAAGCGGATTGTGCTAGCAACTGG
TGGGGCTGGCCAATTATTCCAAGCTACAACCAACCCAACTGTCGCCACTGGCGATGGCGTAGCCTTGGCCTATCGCGCTG
GTGCGGGTGTCTCAAATATGGAGTTCTTCCAATTTCACCCAACGGCTTTGCGGTTGGCTGGCGCACCTGGCTTTTTGATT
TCCGAGGCAGTGCGCGGCGAAGGCGGCTTTTTGCGCAATGTGGCAGGCGAACGCTTTATGCTCAAATTGCACCCCGATGC
TGAATTAGCGCCACGTGATGTGGTTGCACGGGGGATTGCCGCCGAAATGGTTTCAACCGATGCTGATCATGTCTATCTTG
ATGTGACGCACTTGCCAGCCGATTTGGTGCTGTTGCGCTTCCCTACGATTGCCAAAGTCTGTCGCAGCCATGGCTTGGAT
ATTACCAAAGAACCAATTCCGGTTGCGCCTGCTGCTCACTACATGACTGGCGGCGTGGTCACCGATTTGCATGGCCGCAC
CAGCGTGCCCAATTTATTTGCGGTTGGCGAAGTTTCGATGACAGGCGTGCATGGAGCCAACCGTTTGGCCTCGAACTCCT
TGCTCGAGGTGTTGGTTTGGGCCAAACGGGTGATTGAAGCTCCTGATGGCCCAATCGAAGCACCAACCTTTCCCCAAACG
ACCCAAGCGATCACCATGGCCGCCGAAGCCGATGATACGCCATTTAGTCGGGCTGCTTTGCAACGCCTGATGTGGCGCAA
TGTGGGCATTGTGCGCGAAGCCGATGGTTTGGGTTCGGCCTTGCGGCAGTTGGTTGCTTGGCGTGATGCCCAAGCCGAGC
CAGCCACTGTTCGCGAGTATGAAGATGCTAATTTGTTGGTAACCGCAACCCTGTTGACCTATGCCGCCTTATTGCGCGAG
GAAAGCCGTGGAGCGCATGCCCGCCGCGATTTCACTGAAACCCGCGAGGTTTGGCAACGCTACTTGGTCTTGAAGCAAGG
CTAA

Upstream 100 bases:

>100_bases
GCCTTGAGGCGGGACGGAAGGGTGGAGAATCCTAATCTTTCAGCTATAGAATCAAGAACATATATCCTGGTGTTTCATCA
CGAAAGGATTTACCCGTTCT

Downstream 100 bases:

>100_bases
TTATTTATTTACAGCGAGAATGGTGGATCAAACTTGATCCACCATTTTGTATTTAATTATTGCCATACATGTTCATTATT
TGCTAGCACTTTAGCACTTG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNDYIIVGSGIAGLYTAQLAAQHNLKIVLVTKEAVEECNTRYAQGGIAAAIGEHDSPELHIEDTLVAGAGISDPAAVRVL
CEEGPARVHELINLGVPFDTHDGAIALTREAAHSERRILHAGGDATGAAIETTLAGVVANTNVDVRAWTLATEIMLENGQ
AVGVRTLDARTGERGEVRAKRIVLATGGAGQLFQATTNPTVATGDGVALAYRAGAGVSNMEFFQFHPTALRLAGAPGFLI
SEAVRGEGGFLRNVAGERFMLKLHPDAELAPRDVVARGIAAEMVSTDADHVYLDVTHLPADLVLLRFPTIAKVCRSHGLD
ITKEPIPVAPAAHYMTGGVVTDLHGRTSVPNLFAVGEVSMTGVHGANRLASNSLLEVLVWAKRVIEAPDGPIEAPTFPQT
TQAITMAAEADDTPFSRAALQRLMWRNVGIVREADGLGSALRQLVAWRDAQAEPATVREYEDANLLVTATLLTYAALLRE
ESRGAHARRDFTETREVWQRYLVLKQG

Sequences:

>Translated_507_residues
MNDYIIVGSGIAGLYTAQLAAQHNLKIVLVTKEAVEECNTRYAQGGIAAAIGEHDSPELHIEDTLVAGAGISDPAAVRVL
CEEGPARVHELINLGVPFDTHDGAIALTREAAHSERRILHAGGDATGAAIETTLAGVVANTNVDVRAWTLATEIMLENGQ
AVGVRTLDARTGERGEVRAKRIVLATGGAGQLFQATTNPTVATGDGVALAYRAGAGVSNMEFFQFHPTALRLAGAPGFLI
SEAVRGEGGFLRNVAGERFMLKLHPDAELAPRDVVARGIAAEMVSTDADHVYLDVTHLPADLVLLRFPTIAKVCRSHGLD
ITKEPIPVAPAAHYMTGGVVTDLHGRTSVPNLFAVGEVSMTGVHGANRLASNSLLEVLVWAKRVIEAPDGPIEAPTFPQT
TQAITMAAEADDTPFSRAALQRLMWRNVGIVREADGLGSALRQLVAWRDAQAEPATVREYEDANLLVTATLLTYAALLRE
ESRGAHARRDFTETREVWQRYLVLKQG
>Mature_507_residues
MNDYIIVGSGIAGLYTAQLAAQHNLKIVLVTKEAVEECNTRYAQGGIAAAIGEHDSPELHIEDTLVAGAGISDPAAVRVL
CEEGPARVHELINLGVPFDTHDGAIALTREAAHSERRILHAGGDATGAAIETTLAGVVANTNVDVRAWTLATEIMLENGQ
AVGVRTLDARTGERGEVRAKRIVLATGGAGQLFQATTNPTVATGDGVALAYRAGAGVSNMEFFQFHPTALRLAGAPGFLI
SEAVRGEGGFLRNVAGERFMLKLHPDAELAPRDVVARGIAAEMVSTDADHVYLDVTHLPADLVLLRFPTIAKVCRSHGLD
ITKEPIPVAPAAHYMTGGVVTDLHGRTSVPNLFAVGEVSMTGVHGANRLASNSLLEVLVWAKRVIEAPDGPIEAPTFPQT
TQAITMAAEADDTPFSRAALQRLMWRNVGIVREADGLGSALRQLVAWRDAQAEPATVREYEDANLLVTATLLTYAALLRE
ESRGAHARRDFTETREVWQRYLVLKQG

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=518, Percent_Identity=35.1351351351351, Blast_Score=241, Evalue=2e-63,
Organism=Escherichia coli, GI1788928, Length=517, Percent_Identity=42.5531914893617, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1786942, Length=529, Percent_Identity=34.4045368620038, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1790597, Length=509, Percent_Identity=30.0589390962672, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17550100, Length=525, Percent_Identity=36, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17505833, Length=522, Percent_Identity=34.0996168582375, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71986328, Length=457, Percent_Identity=28.6652078774617, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322416, Length=559, Percent_Identity=33.4525939177102, Blast_Score=246, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6322701, Length=540, Percent_Identity=32.037037037037, Blast_Score=237, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6320788, Length=470, Percent_Identity=25.9574468085106, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137288, Length=519, Percent_Identity=34.2967244701349, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24655642, Length=519, Percent_Identity=34.2967244701349, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24655647, Length=519, Percent_Identity=34.2967244701349, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24663005, Length=544, Percent_Identity=32.5367647058824, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 54058; Mature: 54058

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYIIVGSGIAGLYTAQLAAQHNLKIVLVTKEAVEECNTRYAQGGIAAAIGEHDSPELH
CCCEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCEE
IEDTLVAGAGISDPAAVRVLCEEGPARVHELINLGVPFDTHDGAIALTREAAHSERRILH
EEEEEEECCCCCCCHHEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCCCEEEE
AGGDATGAAIETTLAGVVANTNVDVRAWTLATEIMLENGQAVGVRTLDARTGERGEVRAK
ECCCCCCHHHHHHHHHEEECCCCCEEEEEEEEEEHCCCCCEEEEEEECCCCCCCCCEEEE
RIVLATGGAGQLFQATTNPTVATGDGVALAYRAGAGVSNMEFFQFHPTALRLAGAPGFLI
EEEEEECCCCCEEEECCCCEEECCCCEEEEEECCCCCCCCEEEEECCCEEEECCCCCHHH
SEAVRGEGGFLRNVAGERFMLKLHPDAELAPRDVVARGIAAEMVSTDADHVYLDVTHLPA
HHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCH
DLVLLRFPTIAKVCRSHGLDITKEPIPVAPAAHYMTGGVVTDLHGRTSVPNLFAVGEVSM
HEEEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCEEEECCEEE
TGVHGANRLASNSLLEVLVWAKRVIEAPDGPIEAPTFPQTTQAITMAAEADDTPFSRAAL
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
QRLMWRNVGIVREADGLGSALRQLVAWRDAQAEPATVREYEDANLLVTATLLTYAALLRE
HHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCCCHHEECCCCCEEEHHHHHHHHHHHHH
ESRGAHARRDFTETREVWQRYLVLKQG
HCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDYIIVGSGIAGLYTAQLAAQHNLKIVLVTKEAVEECNTRYAQGGIAAAIGEHDSPELH
CCCEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCEE
IEDTLVAGAGISDPAAVRVLCEEGPARVHELINLGVPFDTHDGAIALTREAAHSERRILH
EEEEEEECCCCCCCHHEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCCCEEEE
AGGDATGAAIETTLAGVVANTNVDVRAWTLATEIMLENGQAVGVRTLDARTGERGEVRAK
ECCCCCCHHHHHHHHHEEECCCCCEEEEEEEEEEHCCCCCEEEEEEECCCCCCCCCEEEE
RIVLATGGAGQLFQATTNPTVATGDGVALAYRAGAGVSNMEFFQFHPTALRLAGAPGFLI
EEEEEECCCCCEEEECCCCEEECCCCEEEEEECCCCCCCCEEEEECCCEEEECCCCCHHH
SEAVRGEGGFLRNVAGERFMLKLHPDAELAPRDVVARGIAAEMVSTDADHVYLDVTHLPA
HHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCH
DLVLLRFPTIAKVCRSHGLDITKEPIPVAPAAHYMTGGVVTDLHGRTSVPNLFAVGEVSM
HEEEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCEEEECCEEE
TGVHGANRLASNSLLEVLVWAKRVIEAPDGPIEAPTFPQTTQAITMAAEADDTPFSRAAL
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHH
QRLMWRNVGIVREADGLGSALRQLVAWRDAQAEPATVREYEDANLLVTATLLTYAALLRE
HHHHHHCCCCEEECCHHHHHHHHHHHHCCCCCCCCHHEECCCCCEEEHHHHHHHHHHHHH
ESRGAHARRDFTETREVWQRYLVLKQG
HCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]