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The map label for this gene is hisS [H]

Identifier: 159898767

GI number: 159898767

Start: 2862943

End: 2864247

Strand: Reverse

Name: hisS [H]

Synonym: Haur_2246

Alternate gene names: 159898767

Gene position: 2864247-2862943 (Counterclockwise)

Preceding gene: 159898768

Following gene: 159898766

Centisome position: 45.13

GC content: 53.26

Gene sequence:

>1305_bases
ATGCCAATTACTCCTCGCGCCTACAAAGGCATGCGCGATCACTTGCCCGAAGCTATGCGCTTGCGGCGTTTTATCACCGA
TACCTTGATTGGTATTTTAGAGCGCTATGGCTTTGAGCCACTTTCCACACCGATTGTCGAATATTCGGAAACGCTCGAAG
GCAAGATTGGCGATGAAGAAAAATTGCTGTATCGTTTGAAATATGGCGATGATGCTTTGACCTTGCGTTACGACCAAACT
GTGCCCTTGGCGCGGGTGGTGGCCCAAAACGAAGGCAAATTAACTATGCCCTTCAAACGCTATGCGCTCGGCCAATCGTA
TCGCGGCGAACGCCAAGCTCGTGGTCGCTACCGCGAATTTTGGCAGCTTGATGCCGATATTGTTGGGGTAGATAGCCCGA
TTGCCGATGCTGAAATTGTGGCGGTGGTCGTTGAAGGCTTGCGAGCTTTAGGCTTTACTGGTGCAAAAGTCTTGCTCAAT
CACCGCGAAATCTTGAGTGGGTTGGCGCGGGTAGCAGGTGTGCCCGAAAACGAGGCAGGTGGAGTATATCGCGCGATCGA
TAAGCTCGATAAAATTGGCAATGACGGTGTGCGCAACGAATTGCTCAAAAGTGGCGTGAGTGCCGAAGCTGCTGAGCGTG
TGTTGCACTTCGTCGGGATTAGCGGCTCGATCGAAGCGGTGTTGGCCGAAATGGAAAGCGTGCTAGCCAACGATCCGCCA
GCTTTGGCAGCGGTTGCGGCCTTGCGCACCATCTGCGAGGTATTGACGAGCTTTGGCGTGCCAGCCGATAGTTTTACGAT
TGCGCCTAGCTTGGCTCGCGGCTTATCCTACTATACGGGCTGTGTGTTCGAGGCCGTGCTCGATTCGCCACCGATGGGTT
CGTTATTGGGCGGTGGCCGCTACGACAATTTGGTGGGTATGTTTAGCAAACGCTCGTTGCCGACGGTTGGCTGTGCTTTT
GGGCTTGAACGCTTGTTTGATTTGATGCTTGAGCTTAATATGGGGCCACGCCCAGAGCGCACGATCGATGCCTATGTTAC
CTTATTTGCTGGCGATTTTCAAAATGAGAGCCTGCGCTTGGCGGGCGAATTGCGGGTAGCTGGCCTGAGTGTATTGACCG
CGTATAGTCCAGTCAAAATTGCCAACCAGTTCAAAGAGGCAGATCGCAAGGGTGCGAATTTTGCCTTGGTGCTTGGCCCC
GATGATTTGGCTGCAGGCGTAGTACAGCTCAAAGATTTACGCTCGGGTCAGCAACAAGCTGTAGCGCGTGATGCGATCGT
AGCGGCAATCAAAGCGGCCCAATAA

Upstream 100 bases:

>100_bases
GTTACATGATTACGGCTTTGGGTGAGCTAGATTTAACTGGAGTTTCGCTCAAACGCTAGCGCTTGTGGGCTGATGCCCGT
TTGGATAAGGAACTAAAATT

Downstream 100 bases:

>100_bases
TCCAACTTTTGGCGCTGAGCTAAACCCTCAGCGCCCCAACAGGAACCATCAATGAAGCGATTTCGTGGCTTTCGTCCATC
TCGACTTTCGTTACAACCAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPITPRAYKGMRDHLPEAMRLRRFITDTLIGILERYGFEPLSTPIVEYSETLEGKIGDEEKLLYRLKYGDDALTLRYDQT
VPLARVVAQNEGKLTMPFKRYALGQSYRGERQARGRYREFWQLDADIVGVDSPIADAEIVAVVVEGLRALGFTGAKVLLN
HREILSGLARVAGVPENEAGGVYRAIDKLDKIGNDGVRNELLKSGVSAEAAERVLHFVGISGSIEAVLAEMESVLANDPP
ALAAVAALRTICEVLTSFGVPADSFTIAPSLARGLSYYTGCVFEAVLDSPPMGSLLGGGRYDNLVGMFSKRSLPTVGCAF
GLERLFDLMLELNMGPRPERTIDAYVTLFAGDFQNESLRLAGELRVAGLSVLTAYSPVKIANQFKEADRKGANFALVLGP
DDLAAGVVQLKDLRSGQQQAVARDAIVAAIKAAQ

Sequences:

>Translated_434_residues
MPITPRAYKGMRDHLPEAMRLRRFITDTLIGILERYGFEPLSTPIVEYSETLEGKIGDEEKLLYRLKYGDDALTLRYDQT
VPLARVVAQNEGKLTMPFKRYALGQSYRGERQARGRYREFWQLDADIVGVDSPIADAEIVAVVVEGLRALGFTGAKVLLN
HREILSGLARVAGVPENEAGGVYRAIDKLDKIGNDGVRNELLKSGVSAEAAERVLHFVGISGSIEAVLAEMESVLANDPP
ALAAVAALRTICEVLTSFGVPADSFTIAPSLARGLSYYTGCVFEAVLDSPPMGSLLGGGRYDNLVGMFSKRSLPTVGCAF
GLERLFDLMLELNMGPRPERTIDAYVTLFAGDFQNESLRLAGELRVAGLSVLTAYSPVKIANQFKEADRKGANFALVLGP
DDLAAGVVQLKDLRSGQQQAVARDAIVAAIKAAQ
>Mature_433_residues
PITPRAYKGMRDHLPEAMRLRRFITDTLIGILERYGFEPLSTPIVEYSETLEGKIGDEEKLLYRLKYGDDALTLRYDQTV
PLARVVAQNEGKLTMPFKRYALGQSYRGERQARGRYREFWQLDADIVGVDSPIADAEIVAVVVEGLRALGFTGAKVLLNH
REILSGLARVAGVPENEAGGVYRAIDKLDKIGNDGVRNELLKSGVSAEAAERVLHFVGISGSIEAVLAEMESVLANDPPA
LAAVAALRTICEVLTSFGVPADSFTIAPSLARGLSYYTGCVFEAVLDSPPMGSLLGGGRYDNLVGMFSKRSLPTVGCAFG
LERLFDLMLELNMGPRPERTIDAYVTLFAGDFQNESLRLAGELRVAGLSVLTAYSPVKIANQFKEADRKGANFALVLGPD
DLAAGVVQLKDLRSGQQQAVARDAIVAAIKAAQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=444, Percent_Identity=36.7117117117117, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI15029520, Length=445, Percent_Identity=35.7303370786517, Blast_Score=240, Evalue=2e-63,
Organism=Escherichia coli, GI1788861, Length=445, Percent_Identity=31.0112359550562, Blast_Score=175, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71993686, Length=458, Percent_Identity=32.9694323144105, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI71993693, Length=458, Percent_Identity=32.9694323144105, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6325290, Length=476, Percent_Identity=33.4033613445378, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6320489, Length=312, Percent_Identity=24.6794871794872, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24643061, Length=477, Percent_Identity=34.1719077568134, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24643059, Length=496, Percent_Identity=32.8629032258064, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47028; Mature: 46896

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITPRAYKGMRDHLPEAMRLRRFITDTLIGILERYGFEPLSTPIVEYSETLEGKIGDEE
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHH
KLLYRLKYGDDALTLRYDQTVPLARVVAQNEGKLTMPFKRYALGQSYRGERQARGRYREF
HEEEEEEECCCEEEEEECCCCHHHHHHHCCCCCEECCHHHHHCCCCCCCHHHHHHHHHHH
WQLDADIVGVDSPIADAEIVAVVVEGLRALGFTGAKVLLNHREILSGLARVAGVPENEAG
HHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVYRAIDKLDKIGNDGVRNELLKSGVSAEAAERVLHFVGISGSIEAVLAEMESVLANDPP
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCH
ALAAVAALRTICEVLTSFGVPADSFTIAPSLARGLSYYTGCVFEAVLDSPPMGSLLGGGR
HHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
YDNLVGMFSKRSLPTVGCAFGLERLFDLMLELNMGPRPERTIDAYVTLFAGDFQNESLRL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCEEE
AGELRVAGLSVLTAYSPVKIANQFKEADRKGANFALVLGPDDLAAGVVQLKDLRSGQQQA
EHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHH
VARDAIVAAIKAAQ
HHHHHHHHHHHCCC
>Mature Secondary Structure 
PITPRAYKGMRDHLPEAMRLRRFITDTLIGILERYGFEPLSTPIVEYSETLEGKIGDEE
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHH
KLLYRLKYGDDALTLRYDQTVPLARVVAQNEGKLTMPFKRYALGQSYRGERQARGRYREF
HEEEEEEECCCEEEEEECCCCHHHHHHHCCCCCEECCHHHHHCCCCCCCHHHHHHHHHHH
WQLDADIVGVDSPIADAEIVAVVVEGLRALGFTGAKVLLNHREILSGLARVAGVPENEAG
HHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
GVYRAIDKLDKIGNDGVRNELLKSGVSAEAAERVLHFVGISGSIEAVLAEMESVLANDPP
HHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCH
ALAAVAALRTICEVLTSFGVPADSFTIAPSLARGLSYYTGCVFEAVLDSPPMGSLLGGGR
HHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
YDNLVGMFSKRSLPTVGCAFGLERLFDLMLELNMGPRPERTIDAYVTLFAGDFQNESLRL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCEEE
AGELRVAGLSVLTAYSPVKIANQFKEADRKGANFALVLGPDDLAAGVVQLKDLRSGQQQA
EHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHH
VARDAIVAAIKAAQ
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]