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The map label for this gene is cofH2 [H]

Identifier: 159898684

GI number: 159898684

Start: 2728446

End: 2729549

Strand: Reverse

Name: cofH2 [H]

Synonym: Haur_2163

Alternate gene names: 159898684

Gene position: 2729549-2728446 (Counterclockwise)

Preceding gene: 159898685

Following gene: 159898683

Centisome position: 43.01

GC content: 47.19

Gene sequence:

>1104_bases
ATGGCTATTCTGTTGGCGAAGTCGCCACTCTCTGATATTGCTGAAAAAGTTGAAGCAGGCGAACGCCTTTCGTTTGACGA
TGGAATGCGTTTGTATCAAACTAACGATATTTTAGCCTTGGGTAAATTGGCCGATACGGTGAATCGACGCAAAAATGGCG
ATGTGGTGTATTTTGTGCAAAATCACCGCATTACACCAACCAATGTTTGTGCATTTCACTGTAATTTCTGCTCGTTTCGG
CGTAATGGCAACGAACCCGATGCCTTTGTGCGCACTCCCGAACAAATTATCGATCACGTTGGGCGTTTGTTTAGCGAACG
TACCCGTGAATTTCATATTGTCGGCGGTTTAGTGCCCGATCTCGATGTTGAATATTATGCCGATATCATTCGTGAATTGA
AGGATCATTATCCCAATGTTCACGTCAAAGCCTTTACGGCAGTTGAAATTGATTATATGGCTCAAATTTCGCATCTTGAT
TGGCGCACAACCCTTGAGATTTTGCGCAAGGCTGGGCTGGATGCCTTGCCTGGTGGCGGTGCTGAAATTTTCCATCCAGC
GGTGCGCCGTAAAATCTGCCCCGAAAAGGTTGATGGCGATGGTTGGTTGGAAATTCATGGCATTGCTCACGAATTAGGCA
TCAAAACCAATGCCACTATGCTCTATGGCCATATCGAAACCCTCGAACAACGGGTTGATCACTTGTTACGTTTGCGCGAA
CAGCAAGATAAAACTGGCGGTTTTGTAACCTACATTCCGCTGGCTTTCCACCCCGAAAACAACAATTTAGGGCGGGTCAA
AAAGCTCGATTGGACGACAGGCTTCGAGGATTTGAAGAATTTGGCGATTGGCCGTTTGTTGCTCGACAACTTTGCCCATG
TCAAAGCCTATTGGATCTCGCTCACGCCACGGTTGGCCCAAGTCGCTTTGTCGTTTGGGGTTTCCGATGTTGACGGCACG
GTGATCGAAGAAGAAATCTATCACGCTGCTGGGGCTAAAACCGAACAAGGTATCTCACGGGCAGAATTAGTTCATCTGGT
GACGACTGCTGGCAAAACCGCAGTTGAGCGGGATGCACTTTATAATCACATCGCTGTGAACTAA

Upstream 100 bases:

>100_bases
TGTGCTTTGCCAACTGCTGCCCAAATATGCTGAAAAGTGGCTCGAAAATCAAAATAACATTGTGATATAATGAACACAGT
AGCGCGGAAAGGAACTGTGT

Downstream 100 bases:

>100_bases
TAGCAGGGTTTGGGGCGCTTGCCCAAAGCCTTGCCTATCGCGTGAGTCGCCTAGCGTACTCACGCTTTTAAATTTTATGC
TTGGTGTAATCGATTATCTC

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 2 [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MAILLAKSPLSDIAEKVEAGERLSFDDGMRLYQTNDILALGKLADTVNRRKNGDVVYFVQNHRITPTNVCAFHCNFCSFR
RNGNEPDAFVRTPEQIIDHVGRLFSERTREFHIVGGLVPDLDVEYYADIIRELKDHYPNVHVKAFTAVEIDYMAQISHLD
WRTTLEILRKAGLDALPGGGAEIFHPAVRRKICPEKVDGDGWLEIHGIAHELGIKTNATMLYGHIETLEQRVDHLLRLRE
QQDKTGGFVTYIPLAFHPENNNLGRVKKLDWTTGFEDLKNLAIGRLLLDNFAHVKAYWISLTPRLAQVALSFGVSDVDGT
VIEEEIYHAAGAKTEQGISRAELVHLVTTAGKTAVERDALYNHIAVN

Sequences:

>Translated_367_residues
MAILLAKSPLSDIAEKVEAGERLSFDDGMRLYQTNDILALGKLADTVNRRKNGDVVYFVQNHRITPTNVCAFHCNFCSFR
RNGNEPDAFVRTPEQIIDHVGRLFSERTREFHIVGGLVPDLDVEYYADIIRELKDHYPNVHVKAFTAVEIDYMAQISHLD
WRTTLEILRKAGLDALPGGGAEIFHPAVRRKICPEKVDGDGWLEIHGIAHELGIKTNATMLYGHIETLEQRVDHLLRLRE
QQDKTGGFVTYIPLAFHPENNNLGRVKKLDWTTGFEDLKNLAIGRLLLDNFAHVKAYWISLTPRLAQVALSFGVSDVDGT
VIEEEIYHAAGAKTEQGISRAELVHLVTTAGKTAVERDALYNHIAVN
>Mature_366_residues
AILLAKSPLSDIAEKVEAGERLSFDDGMRLYQTNDILALGKLADTVNRRKNGDVVYFVQNHRITPTNVCAFHCNFCSFRR
NGNEPDAFVRTPEQIIDHVGRLFSERTREFHIVGGLVPDLDVEYYADIIRELKDHYPNVHVKAFTAVEIDYMAQISHLDW
RTTLEILRKAGLDALPGGGAEIFHPAVRRKICPEKVDGDGWLEIHGIAHELGIKTNATMLYGHIETLEQRVDHLLRLREQ
QDKTGGFVTYIPLAFHPENNNLGRVKKLDWTTGFEDLKNLAIGRLLLDNFAHVKAYWISLTPRLAQVALSFGVSDVDGTV
IEEEIYHAAGAKTEQGISRAELVHLVTTAGKTAVERDALYNHIAVN

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41269; Mature: 41138

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILLAKSPLSDIAEKVEAGERLSFDDGMRLYQTNDILALGKLADTVNRRKNGDVVYFVQ
CEEEEECCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEHHHHHHHHHCCCCCCEEEEEE
NHRITPTNVCAFHCNFCSFRRNGNEPDAFVRTPEQIIDHVGRLFSERTREFHIVGGLVPD
CCCCCCCCEEEEEECHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCC
LDVEYYADIIRELKDHYPNVHVKAFTAVEIDYMAQISHLDWRTTLEILRKAGLDALPGGG
CCHHHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
AEIFHPAVRRKICPEKVDGDGWLEIHGIAHELGIKTNATMLYGHIETLEQRVDHLLRLRE
HHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHH
QQDKTGGFVTYIPLAFHPENNNLGRVKKLDWTTGFEDLKNLAIGRLLLDNFAHVKAYWIS
HCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEE
LTPRLAQVALSFGVSDVDGTVIEEEIYHAAGAKTEQGISRAELVHLVTTAGKTAVERDAL
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHH
YNHIAVN
HHHHCCC
>Mature Secondary Structure 
AILLAKSPLSDIAEKVEAGERLSFDDGMRLYQTNDILALGKLADTVNRRKNGDVVYFVQ
EEEEECCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEHHHHHHHHHCCCCCCEEEEEE
NHRITPTNVCAFHCNFCSFRRNGNEPDAFVRTPEQIIDHVGRLFSERTREFHIVGGLVPD
CCCCCCCCEEEEEECHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCC
LDVEYYADIIRELKDHYPNVHVKAFTAVEIDYMAQISHLDWRTTLEILRKAGLDALPGGG
CCHHHHHHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
AEIFHPAVRRKICPEKVDGDGWLEIHGIAHELGIKTNATMLYGHIETLEQRVDHLLRLRE
HHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHH
QQDKTGGFVTYIPLAFHPENNNLGRVKKLDWTTGFEDLKNLAIGRLLLDNFAHVKAYWIS
HCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEEE
LTPRLAQVALSFGVSDVDGTVIEEEIYHAAGAKTEQGISRAELVHLVTTAGKTAVERDAL
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHH
YNHIAVN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA