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The map label for this gene is gacS [H]

Identifier: 159898664

GI number: 159898664

Start: 2699207

End: 2701474

Strand: Direct

Name: gacS [H]

Synonym: Haur_2143

Alternate gene names: 159898664

Gene position: 2699207-2701474 (Clockwise)

Preceding gene: 159898662

Following gene: 159898665

Centisome position: 42.53

GC content: 46.74

Gene sequence:

>2268_bases
ATGCAAGCCTCAATCCAGCGCTGGCTTAATACCATTAAAATCTCCGACCCTGTGCAACGTGAACAAGCTGTCCTCCTTCA
AATCATGCTGATTGGATTAAGCATCATTTGTTTCTTCACAGTTCCCACTGCATTTATTGTGTCAAGTACTGTTGGCAGTG
CATTTCGTGCCAGTCTAACAAGTATATTGATTTTAATCTTCTTTGTAATCGCCGTCATGAATTTGCGTCAAGGCCATTTT
CGGCGCTCAGTTACACTGGCATCAAGTGGTCTAGTCGTTGGTTTGTCGATTATTCTTTTACTTGAAGGTTTACGCAATAG
TGCTTGGATTCTTTCGGCTTTCATGATGCCAATCACGCTATCGGGTTTGTTGACTGGCCGCAAGGGTATTAAAACAATTG
CTGGTGCCAGTATTATCGTAATCGCCATCCTTGGCGGATTACAGCTATTTAATCTGCAATTTGTTGGGGCTTATCCGCGC
CTCGGCGATCTTTTGCCACCAACCATTAGCACGTTTATATTTAGTGTGTTGATTGTAGGCTTCTTCTTTCATCGTTTTAG
CGCCTCGTTAAATGATGCCCTCAGCCGCGCTTTGCAACGTGAATCCGAACTCCAACAGATTCGCGATAAACTTGAAATTC
TGGTTGATCAGCGAACCGCAGCCTTGCAAGATGCTTTACACGCGGTCGAAGAGCGTGAAGGTCGGCTCGAAAAAACCAAT
ATCGAGCTAGCCGCCGCCAAAACTGCTGCCGAGGAAGCTAATCAACTCAAGTCGCGCTTCTTGGCCAATATGAGCCACGA
GTTGCGCACCCCGCTCAATGCAATTATTAACTTCACGGCCTTTCTTGATCGGTATGGTGAGTTCTCTGAGCGCCAACGTG
AGCTGCAAGAACGGGTGCTCTACAACGCTGATCATTTGCTTGGTTTGATCAACGACATTCTCGACCTCTCGAAAATTGAG
GCTGGACGCATGGAGTTGATGTATGAGAAAACTCAACTTGAGCCAATCATCACTGGGGTCATGGCGACTGCCAGCGGGCT
AACCCGTGATAAAGGGCTTGAGCTTGAACTCGATATGCCCGAAGAACTACCAACGATGGTGATTGATAAAGTGCGGATTC
GCCAAGTATTGCTCAATTTGCTTTCAAACGCCGTCAAATTTACTGAGCAAGGCCAAATTAATGTGACGATCAGCCAGCCC
GATCCTGAAACTGTCCAAATTTCCGTCCAAGATAGTGGAATTGGGATCGCTCCTGAGCATCAACAACAAATTTTCGAAGA
ATTTCAACAAGTGCAAAATGAAGTTACCCACCAATATCAAGGCACTGGTTTAGGCCTGCCAATTAGTAAATTGTTGGTGG
AGATGCACGGCGGCAATATGTGGCTCGAAAGCACGCCAGGCAAAGGTTCAACCTTCTTCTTTAGTTTGCCGATTACCAAT
AAATTACCCGAAACGACCACGATTGATTTAACTGCCCCACCAAGTTTGGCCGAACTTGAGGCAGAACCAAGCAACCCAAC
AACCATTGAGATTGTGGTGATTGATGATAATCCTGATGCCCATGAGACCTTCCGGGTCATGCTAGAATCAGGAGGGTATC
GCGTGCATAGCGTGCTTGATAGTCGCTTGGCAATCAGCACAATCAAACAAATTGAGCCACGCTTGATTATTACCGATGTG
CAAATGCCCAATCTTGATGGCTGGGAATTGCTGGCCCAGATCAAGAATGATCCAACGATTGCCCATATTCCCATCGTCGT
CTGCTCAGTTGTTGATCAAGGGACGATTGGGCTGGTTCTAGGGGCAAGGAAACATATTGTTAAACCTGTACGTGAGGAGG
TATTGCTGGCTGTTGTTCAAGAATGTGTCGAACCAACTGCCGAGATCTTGGTCGTTGATGATAACCCTGATGCTCGTCAA
GTGATTCAGCACATCCTCGCAGCACGTGATTATGCCATCAGTGAGGCCGCCGATGGGATCGAAGCGATTGCCATGATCGA
ACAATCAAAACCCGATCTGGTCATCCTTGATCTCATGATGCCACAAATGGATGGCTTTGAAGTGCTCGAAAACCTGCGGC
ATAGCCCTAATTATGCCGATATTCCGGTGATTATTGTCAGCGCCAAGGATCTTGATCAACAGGAACATGATTGGCTGCGC
ACTCGCGCCCAAGGGGTCATTGCTAAACGCCAACTTAGTGAGGAAGAATTTCTTAGACGCATTAATGAGATCTTTAACCA
AGGAGTAGAGTATGCCCGTGGCAAGTAA

Upstream 100 bases:

>100_bases
TTTGCTCCGCGCATCAATTCAAGCAGATCATTGTGGTTGCTGATGGAAGAGTCGTTAATCTTTGGCAGTGCATAGCAGGC
GCTATGCTGAAGGAGCAAGC

Downstream 100 bases:

>100_bases
TGAATATTTTGTTCCCGTTGTACCGAGCGACGCATCTGTGATTATTGTTGAGGATAACCCCGACAATTTGTTCGTTTTGG
AGATTCTGCTCCGCGAAGAT

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MQASIQRWLNTIKISDPVQREQAVLLQIMLIGLSIICFFTVPTAFIVSSTVGSAFRASLTSILILIFFVIAVMNLRQGHF
RRSVTLASSGLVVGLSIILLLEGLRNSAWILSAFMMPITLSGLLTGRKGIKTIAGASIIVIAILGGLQLFNLQFVGAYPR
LGDLLPPTISTFIFSVLIVGFFFHRFSASLNDALSRALQRESELQQIRDKLEILVDQRTAALQDALHAVEEREGRLEKTN
IELAAAKTAAEEANQLKSRFLANMSHELRTPLNAIINFTAFLDRYGEFSERQRELQERVLYNADHLLGLINDILDLSKIE
AGRMELMYEKTQLEPIITGVMATASGLTRDKGLELELDMPEELPTMVIDKVRIRQVLLNLLSNAVKFTEQGQINVTISQP
DPETVQISVQDSGIGIAPEHQQQIFEEFQQVQNEVTHQYQGTGLGLPISKLLVEMHGGNMWLESTPGKGSTFFFSLPITN
KLPETTTIDLTAPPSLAELEAEPSNPTTIEIVVIDDNPDAHETFRVMLESGGYRVHSVLDSRLAISTIKQIEPRLIITDV
QMPNLDGWELLAQIKNDPTIAHIPIVVCSVVDQGTIGLVLGARKHIVKPVREEVLLAVVQECVEPTAEILVVDDNPDARQ
VIQHILAARDYAISEAADGIEAIAMIEQSKPDLVILDLMMPQMDGFEVLENLRHSPNYADIPVIIVSAKDLDQQEHDWLR
TRAQGVIAKRQLSEEEFLRRINEIFNQGVEYARGK

Sequences:

>Translated_755_residues
MQASIQRWLNTIKISDPVQREQAVLLQIMLIGLSIICFFTVPTAFIVSSTVGSAFRASLTSILILIFFVIAVMNLRQGHF
RRSVTLASSGLVVGLSIILLLEGLRNSAWILSAFMMPITLSGLLTGRKGIKTIAGASIIVIAILGGLQLFNLQFVGAYPR
LGDLLPPTISTFIFSVLIVGFFFHRFSASLNDALSRALQRESELQQIRDKLEILVDQRTAALQDALHAVEEREGRLEKTN
IELAAAKTAAEEANQLKSRFLANMSHELRTPLNAIINFTAFLDRYGEFSERQRELQERVLYNADHLLGLINDILDLSKIE
AGRMELMYEKTQLEPIITGVMATASGLTRDKGLELELDMPEELPTMVIDKVRIRQVLLNLLSNAVKFTEQGQINVTISQP
DPETVQISVQDSGIGIAPEHQQQIFEEFQQVQNEVTHQYQGTGLGLPISKLLVEMHGGNMWLESTPGKGSTFFFSLPITN
KLPETTTIDLTAPPSLAELEAEPSNPTTIEIVVIDDNPDAHETFRVMLESGGYRVHSVLDSRLAISTIKQIEPRLIITDV
QMPNLDGWELLAQIKNDPTIAHIPIVVCSVVDQGTIGLVLGARKHIVKPVREEVLLAVVQECVEPTAEILVVDDNPDARQ
VIQHILAARDYAISEAADGIEAIAMIEQSKPDLVILDLMMPQMDGFEVLENLRHSPNYADIPVIIVSAKDLDQQEHDWLR
TRAQGVIAKRQLSEEEFLRRINEIFNQGVEYARGK
>Mature_755_residues
MQASIQRWLNTIKISDPVQREQAVLLQIMLIGLSIICFFTVPTAFIVSSTVGSAFRASLTSILILIFFVIAVMNLRQGHF
RRSVTLASSGLVVGLSIILLLEGLRNSAWILSAFMMPITLSGLLTGRKGIKTIAGASIIVIAILGGLQLFNLQFVGAYPR
LGDLLPPTISTFIFSVLIVGFFFHRFSASLNDALSRALQRESELQQIRDKLEILVDQRTAALQDALHAVEEREGRLEKTN
IELAAAKTAAEEANQLKSRFLANMSHELRTPLNAIINFTAFLDRYGEFSERQRELQERVLYNADHLLGLINDILDLSKIE
AGRMELMYEKTQLEPIITGVMATASGLTRDKGLELELDMPEELPTMVIDKVRIRQVLLNLLSNAVKFTEQGQINVTISQP
DPETVQISVQDSGIGIAPEHQQQIFEEFQQVQNEVTHQYQGTGLGLPISKLLVEMHGGNMWLESTPGKGSTFFFSLPITN
KLPETTTIDLTAPPSLAELEAEPSNPTTIEIVVIDDNPDAHETFRVMLESGGYRVHSVLDSRLAISTIKQIEPRLIITDV
QMPNLDGWELLAQIKNDPTIAHIPIVVCSVVDQGTIGLVLGARKHIVKPVREEVLLAVVQECVEPTAEILVVDDNPDARQ
VIQHILAARDYAISEAADGIEAIAMIEQSKPDLVILDLMMPQMDGFEVLENLRHSPNYADIPVIIVSAKDLDQQEHDWLR
TRAQGVIAKRQLSEEEFLRRINEIFNQGVEYARGK

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=542, Percent_Identity=30.9963099630996, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788713, Length=384, Percent_Identity=31.5104166666667, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI87081816, Length=423, Percent_Identity=32.1513002364066, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI48994928, Length=476, Percent_Identity=28.1512605042017, Blast_Score=143, Evalue=5e-35,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=35.9183673469388, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI1786912, Length=235, Percent_Identity=31.9148936170213, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=33.1838565022422, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=32.3008849557522, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788393, Length=260, Percent_Identity=29.6153846153846, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786783, Length=232, Percent_Identity=30.1724137931034, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=30.4932735426009, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=29.0598290598291, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082128, Length=225, Percent_Identity=28.8888888888889, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789808, Length=231, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=232, Percent_Identity=25, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322044, Length=387, Percent_Identity=24.5478036175711, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 83894; Mature: 83894

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASIQRWLNTIKISDPVQREQAVLLQIMLIGLSIICFFTVPTAFIVSSTVGSAFRASLT
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILILIFFVIAVMNLRQGHFRRSVTLASSGLVVGLSIILLLEGLRNSAWILSAFMMPITL
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SGLLTGRKGIKTIAGASIIVIAILGGLQLFNLQFVGAYPRLGDLLPPTISTFIFSVLIVG
HHHHCCCHHHHHHHCHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
FFFHRFSASLNDALSRALQRESELQQIRDKLEILVDQRTAALQDALHAVEEREGRLEKTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IELAAAKTAAEEANQLKSRFLANMSHELRTPLNAIINFTAFLDRYGEFSERQRELQERVL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YNADHLLGLINDILDLSKIEAGRMELMYEKTQLEPIITGVMATASGLTRDKGLELELDMP
HCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
EELPTMVIDKVRIRQVLLNLLSNAVKFTEQGQINVTISQPDPETVQISVQDSGIGIAPEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCHH
QQQIFEEFQQVQNEVTHQYQGTGLGLPISKLLVEMHGGNMWLESTPGKGSTFFFSLPITN
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCC
KLPETTTIDLTAPPSLAELEAEPSNPTTIEIVVIDDNPDAHETFRVMLESGGYRVHSVLD
CCCCCEEEEEECCCCHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHH
SRLAISTIKQIEPRLIITDVQMPNLDGWELLAQIKNDPTIAHIPIVVCSVVDQGTIGLVL
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEEE
GARKHIVKPVREEVLLAVVQECVEPTAEILVVDDNPDARQVIQHILAARDYAISEAADGI
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
EAIAMIEQSKPDLVILDLMMPQMDGFEVLENLRHSPNYADIPVIIVSAKDLDQQEHDWLR
HHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHH
TRAQGVIAKRQLSEEEFLRRINEIFNQGVEYARGK
HHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCC
>Mature Secondary Structure
MQASIQRWLNTIKISDPVQREQAVLLQIMLIGLSIICFFTVPTAFIVSSTVGSAFRASLT
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILILIFFVIAVMNLRQGHFRRSVTLASSGLVVGLSIILLLEGLRNSAWILSAFMMPITL
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SGLLTGRKGIKTIAGASIIVIAILGGLQLFNLQFVGAYPRLGDLLPPTISTFIFSVLIVG
HHHHCCCHHHHHHHCHHHHHHHHHCCHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
FFFHRFSASLNDALSRALQRESELQQIRDKLEILVDQRTAALQDALHAVEEREGRLEKTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IELAAAKTAAEEANQLKSRFLANMSHELRTPLNAIINFTAFLDRYGEFSERQRELQERVL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YNADHLLGLINDILDLSKIEAGRMELMYEKTQLEPIITGVMATASGLTRDKGLELELDMP
HCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
EELPTMVIDKVRIRQVLLNLLSNAVKFTEQGQINVTISQPDPETVQISVQDSGIGIAPEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCHH
QQQIFEEFQQVQNEVTHQYQGTGLGLPISKLLVEMHGGNMWLESTPGKGSTFFFSLPITN
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCC
KLPETTTIDLTAPPSLAELEAEPSNPTTIEIVVIDDNPDAHETFRVMLESGGYRVHSVLD
CCCCCEEEEEECCCCHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHH
SRLAISTIKQIEPRLIITDVQMPNLDGWELLAQIKNDPTIAHIPIVVCSVVDQGTIGLVL
HHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEEE
GARKHIVKPVREEVLLAVVQECVEPTAEILVVDDNPDARQVIQHILAARDYAISEAADGI
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
EAIAMIEQSKPDLVILDLMMPQMDGFEVLENLRHSPNYADIPVIIVSAKDLDQQEHDWLR
HHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHH
TRAQGVIAKRQLSEEEFLRRINEIFNQGVEYARGK
HHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]