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The map label for this gene is lip2 [H]

Identifier: 159898661

GI number: 159898661

Start: 2694452

End: 2695372

Strand: Reverse

Name: lip2 [H]

Synonym: Haur_2140

Alternate gene names: 159898661

Gene position: 2695372-2694452 (Counterclockwise)

Preceding gene: 159898663

Following gene: 159898660

Centisome position: 42.47

GC content: 52.23

Gene sequence:

>921_bases
ATGCAGCTTTCAATAGTCGCTCCTGAATTGCAACGCTATGTCCGCCGCATTCCACCTTTGCCGTTGGGCAGCGCTTGGGG
ACGGCGTTTTGTACGTTGGTTAGGCAGTCGCATTCCGGCCAAGCAGTTTGATGGAGTGACGATTGAGCAACGCAACGATT
TGAATCCCCCAGTACGCATCTATCGCCCCACTGTTTGTCACTCAACGGCTGCCTTGTTTTGGATTCATGGCGGCGGGATG
ATTATTGGTCAGGCGGCACAAGATGATCAATGGTGTGCCACGACGGCGCAACGCTTAGGAATTGTGGTGGTTTCGGTGGA
ATATCGATTGGCTCCCGAACACCCGTTTCCTGCGCCGCTTGACGATTGTTTGGCTGGTTGGCAGTGGCTTCAGCAGCAGG
CCACGACCCTAGCGATTGATCCAGCGCGGGTGGTGCTTGGTGGCGAAAGTGCTGGTGGTGGCTTGGCGGCAAGTTTGGCG
CAACGCTTGGTTGATCTTGGCACGACCCAGCCTCTGGCTCAATTGCTATTTTGCCCAATGCTTGATGACCGCACAACCAC
CAAGGCTGAATTGACTGCAATTGAGCATTTTGTCTGGAATAACCGCAAAAATGTGATTGGCTGGCAAGCCTATTTGGCGA
CCAAACCTGGCATTGATGCAATTCCAGCCTATGCAGTGCCTGCGCGACGCGAAAACCTGCAAGGCTTGCCTCCCACTTGG
ATCGGTGTTGGCGATATTGATTTGTTTTACCAAGAAGATCGTTTGTATGCTGAACGTTTACAAGTAGTTGACGTTGACGT
AACGTTTGAGATTGTAGCTGGTGGGCCGCATGGTTTTCAGAATTGGGCCTTTGCTAGTGCAATTGCCCAAGCGTTTATTG
CCAAAGCCCATGCGTGGATTCAAACACAGATTGGATGCTAG

Upstream 100 bases:

>100_bases
TTTTTGCTGGTAAATCACTGCCATTTACTGTACAAATTGGGCTTAATCTGCTATTTAGCAGGTTAATTGAGCATGATTTT
TGGCTTGAAAGGGGTTGTCG

Downstream 100 bases:

>100_bases
TTGCAGCCGTTTGGGTATAAGTAGAATGATGAATCTCAGCGATAAGGAATTGATGGATGGCTTCTAATGCTGTTATTGTT
GTCATCACCGGTGCGCCTGG

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQLSIVAPELQRYVRRIPPLPLGSAWGRRFVRWLGSRIPAKQFDGVTIEQRNDLNPPVRIYRPTVCHSTAALFWIHGGGM
IIGQAAQDDQWCATTAQRLGIVVVSVEYRLAPEHPFPAPLDDCLAGWQWLQQQATTLAIDPARVVLGGESAGGGLAASLA
QRLVDLGTTQPLAQLLFCPMLDDRTTTKAELTAIEHFVWNNRKNVIGWQAYLATKPGIDAIPAYAVPARRENLQGLPPTW
IGVGDIDLFYQEDRLYAERLQVVDVDVTFEIVAGGPHGFQNWAFASAIAQAFIAKAHAWIQTQIGC

Sequences:

>Translated_306_residues
MQLSIVAPELQRYVRRIPPLPLGSAWGRRFVRWLGSRIPAKQFDGVTIEQRNDLNPPVRIYRPTVCHSTAALFWIHGGGM
IIGQAAQDDQWCATTAQRLGIVVVSVEYRLAPEHPFPAPLDDCLAGWQWLQQQATTLAIDPARVVLGGESAGGGLAASLA
QRLVDLGTTQPLAQLLFCPMLDDRTTTKAELTAIEHFVWNNRKNVIGWQAYLATKPGIDAIPAYAVPARRENLQGLPPTW
IGVGDIDLFYQEDRLYAERLQVVDVDVTFEIVAGGPHGFQNWAFASAIAQAFIAKAHAWIQTQIGC
>Mature_306_residues
MQLSIVAPELQRYVRRIPPLPLGSAWGRRFVRWLGSRIPAKQFDGVTIEQRNDLNPPVRIYRPTVCHSTAALFWIHGGGM
IIGQAAQDDQWCATTAQRLGIVVVSVEYRLAPEHPFPAPLDDCLAGWQWLQQQATTLAIDPARVVLGGESAGGGLAASLA
QRLVDLGTTQPLAQLLFCPMLDDRTTTKAELTAIEHFVWNNRKNVIGWQAYLATKPGIDAIPAYAVPARRENLQGLPPTW
IGVGDIDLFYQEDRLYAERLQVVDVDVTFEIVAGGPHGFQNWAFASAIAQAFIAKAHAWIQTQIGC

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=332, Percent_Identity=26.5060240963855, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI206597554, Length=298, Percent_Identity=27.5167785234899, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI157041239, Length=111, Percent_Identity=35.1351351351351, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68051721, Length=279, Percent_Identity=25.4480286738351, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI61966717, Length=145, Percent_Identity=28.2758620689655, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21328446, Length=104, Percent_Identity=32.6923076923077, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=223, Percent_Identity=25.1121076233184, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=128, Percent_Identity=39.84375, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17540028, Length=129, Percent_Identity=35.6589147286822, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=153, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=143, Percent_Identity=35.6643356643357, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533410, Length=103, Percent_Identity=33.0097087378641, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=111, Percent_Identity=31.5315315315315, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656084, Length=115, Percent_Identity=33.0434782608696, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656076, Length=115, Percent_Identity=33.0434782608696, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130169, Length=115, Percent_Identity=33.0434782608696, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 33753; Mature: 33753

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSIVAPELQRYVRRIPPLPLGSAWGRRFVRWLGSRIPAKQFDGVTIEQRNDLNPPVRI
CEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCCEEE
YRPTVCHSTAALFWIHGGGMIIGQAAQDDQWCATTAQRLGIVVVSVEYRLAPEHPFPAPL
ECCHHHCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
DDCLAGWQWLQQQATTLAIDPARVVLGGESAGGGLAASLAQRLVDLGTTQPLAQLLFCPM
HHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCC
LDDRTTTKAELTAIEHFVWNNRKNVIGWQAYLATKPGIDAIPAYAVPARRENLQGLPPTW
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCE
IGVGDIDLFYQEDRLYAERLQVVDVDVTFEIVAGGPHGFQNWAFASAIAQAFIAKAHAWI
EECCCEEEEEEHHHHHHHHEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QTQIGC
EEECCC
>Mature Secondary Structure
MQLSIVAPELQRYVRRIPPLPLGSAWGRRFVRWLGSRIPAKQFDGVTIEQRNDLNPPVRI
CEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCEEEECCCCCCCCEEE
YRPTVCHSTAALFWIHGGGMIIGQAAQDDQWCATTAQRLGIVVVSVEYRLAPEHPFPAPL
ECCHHHCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
DDCLAGWQWLQQQATTLAIDPARVVLGGESAGGGLAASLAQRLVDLGTTQPLAQLLFCPM
HHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCC
LDDRTTTKAELTAIEHFVWNNRKNVIGWQAYLATKPGIDAIPAYAVPARRENLQGLPPTW
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCE
IGVGDIDLFYQEDRLYAERLQVVDVDVTFEIVAGGPHGFQNWAFASAIAQAFIAKAHAWI
EECCCEEEEEEHHHHHHHHEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
QTQIGC
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]