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The map label for this gene is ansA [H]

Identifier: 159898361

GI number: 159898361

Start: 2136393

End: 2137358

Strand: Direct

Name: ansA [H]

Synonym: Haur_1837

Alternate gene names: 159898361

Gene position: 2136393-2137358 (Clockwise)

Preceding gene: 159898360

Following gene: 159898374

Centisome position: 33.66

GC content: 52.38

Gene sequence:

>966_bases
ATGCACAACGTTGTGGTTATTACAACGGGCGGCACGATTTCGACCAAACGCACACCGCAATGGAGCGGCGCAGTTCCAAT
GCTCAAAGGCGATGATTTTTCGGCGATGCTCCCACGCGGCGAGTTTAATGTTAGCTTTCAAGATTTTACCAATTTGCCAA
GTAGCCATATTACCCCAACCATGGCCCTCGATTTAGCGCAACGGGTCAATAGTTTGTTGCTAGGCGAGGCTGATGGGGTG
GTGGTGACCCATGGCACCGATACCTTGGAAGAAAGCGCCTTTTTATGCGATTTGTTGATCGATTCGAATAAACCGGTGGT
GTTTACTGGCTCGATGCGGATTGCTACCGATGTGGGTTATGATGGCGTGACCAATTTAACTGGGGCGATTCGCGCCGCCG
CTGCTCCGCAAAGCCGTGGGCTTGGCGTGATGGTCGCTTTCAATGATCGTTTGTTTTCAGCTTCGACGGTGCAAAAAATC
GATTCACAAACTGCTGATGCCTTCGATGCGCCTGGTTTTGGCCCCGTTGGCAGTTTGACAGGCGCAACCGTGCGGATTCA
TCATCAGCCCAGCGGTCGCCAATATATCCCATGTTCGCGGCTTGCTGAGCGAGTTGATTTAATTTATGCCACTCAAGGAG
CCGATGATCGTCAACTGCGGGCGGCGATTGATACCAATTCACAAGGCATCGTGATTGCGGCGTTTGGTGGTGGGCGCGTG
CCGCCGTGGTGGTTGCCAACCATCCAAGAAGCGCTCAACAAACGGATTCCGGTGGTGGTGACGACGCGCTGCTTGAAGGG
CATTTGCTACGATGAATATGGCTATGTTGGCGAATATCATGATCTCAAACGGCTTGGTGTGATGTTTGCTCAGCAGCTTT
CGCCAGTCAAAGCACGCATCAAATTGATGGTTGCCTTGGGTGCAGCCGCCCATCCCCAAGAATTGCGTGAGTGGTTTGCT
GAATAA

Upstream 100 bases:

>100_bases
CCCCTCGCCCTTCAATCCTCGCTTGACGTGAGCAGCAAGCTTCATTACACTCAATTGCTGAAGCCACTTACGATTCACAG
CCTTCGGGCAGGGAGACTAT

Downstream 100 bases:

>100_bases
TTCCTAAAATAAGTACGCCCTGCTCAGTTATTATAATGATGCAGGGCGTACTCACGTTTACGTTTAATTGTAAGAATTAG
GGAAATCGGCCTGCCAATGC

Product: asparaginase/glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MHNVVVITTGGTISTKRTPQWSGAVPMLKGDDFSAMLPRGEFNVSFQDFTNLPSSHITPTMALDLAQRVNSLLLGEADGV
VVTHGTDTLEESAFLCDLLIDSNKPVVFTGSMRIATDVGYDGVTNLTGAIRAAAAPQSRGLGVMVAFNDRLFSASTVQKI
DSQTADAFDAPGFGPVGSLTGATVRIHHQPSGRQYIPCSRLAERVDLIYATQGADDRQLRAAIDTNSQGIVIAAFGGGRV
PPWWLPTIQEALNKRIPVVVTTRCLKGICYDEYGYVGEYHDLKRLGVMFAQQLSPVKARIKLMVALGAAAHPQELREWFA
E

Sequences:

>Translated_321_residues
MHNVVVITTGGTISTKRTPQWSGAVPMLKGDDFSAMLPRGEFNVSFQDFTNLPSSHITPTMALDLAQRVNSLLLGEADGV
VVTHGTDTLEESAFLCDLLIDSNKPVVFTGSMRIATDVGYDGVTNLTGAIRAAAAPQSRGLGVMVAFNDRLFSASTVQKI
DSQTADAFDAPGFGPVGSLTGATVRIHHQPSGRQYIPCSRLAERVDLIYATQGADDRQLRAAIDTNSQGIVIAAFGGGRV
PPWWLPTIQEALNKRIPVVVTTRCLKGICYDEYGYVGEYHDLKRLGVMFAQQLSPVKARIKLMVALGAAAHPQELREWFA
E
>Mature_321_residues
MHNVVVITTGGTISTKRTPQWSGAVPMLKGDDFSAMLPRGEFNVSFQDFTNLPSSHITPTMALDLAQRVNSLLLGEADGV
VVTHGTDTLEESAFLCDLLIDSNKPVVFTGSMRIATDVGYDGVTNLTGAIRAAAAPQSRGLGVMVAFNDRLFSASTVQKI
DSQTADAFDAPGFGPVGSLTGATVRIHHQPSGRQYIPCSRLAERVDLIYATQGADDRQLRAAIDTNSQGIVIAAFGGGRV
PPWWLPTIQEALNKRIPVVVTTRCLKGICYDEYGYVGEYHDLKRLGVMFAQQLSPVKARIKLMVALGAAAHPQELREWFA
E

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=285, Percent_Identity=30.1754385964912, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788065, Length=278, Percent_Identity=26.2589928057554, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558278, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=343, Percent_Identity=29.4460641399417, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323187, Length=343, Percent_Identity=29.4460641399417, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323186, Length=343, Percent_Identity=29.4460641399417, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323184, Length=343, Percent_Identity=29.4460641399417, Blast_Score=119, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320527, Length=332, Percent_Identity=29.2168674698795, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24645530, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639609, Length=249, Percent_Identity=27.3092369477912, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34633; Mature: 34633

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNVVVITTGGTISTKRTPQWSGAVPMLKGDDFSAMLPRGEFNVSFQDFTNLPSSHITPT
CCCEEEEEECCEEECCCCCCCCCCCCEEECCCCCEECCCCCCEEEHHHHHCCCCCCCCHH
MALDLAQRVNSLLLGEADGVVVTHGTDTLEESAFLCDLLIDSNKPVVFTGSMRIATDVGY
HHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHEEEEEEECCCCCEEEECCEEEEEECCC
DGVTNLTGAIRAAAAPQSRGLGVMVAFNDRLFSASTVQKIDSQTADAFDAPGFGPVGSLT
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
GATVRIHHQPSGRQYIPCSRLAERVDLIYATQGADDRQLRAAIDTNSQGIVIAAFGGGRV
CCEEEEEECCCCCEECCHHHHHHHHEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCC
PPWWLPTIQEALNKRIPVVVTTRCLKGICYDEYGYVGEYHDLKRLGVMFAQQLSPVKARI
CCCCCHHHHHHHHCCCCEEEEHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHE
KLMVALGAAAHPQELREWFAE
EEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MHNVVVITTGGTISTKRTPQWSGAVPMLKGDDFSAMLPRGEFNVSFQDFTNLPSSHITPT
CCCEEEEEECCEEECCCCCCCCCCCCEEECCCCCEECCCCCCEEEHHHHHCCCCCCCCHH
MALDLAQRVNSLLLGEADGVVVTHGTDTLEESAFLCDLLIDSNKPVVFTGSMRIATDVGY
HHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHEEEEEEECCCCCEEEECCEEEEEECCC
DGVTNLTGAIRAAAAPQSRGLGVMVAFNDRLFSASTVQKIDSQTADAFDAPGFGPVGSLT
CCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
GATVRIHHQPSGRQYIPCSRLAERVDLIYATQGADDRQLRAAIDTNSQGIVIAAFGGGRV
CCEEEEEECCCCCEECCHHHHHHHHEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCC
PPWWLPTIQEALNKRIPVVVTTRCLKGICYDEYGYVGEYHDLKRLGVMFAQQLSPVKARI
CCCCCHHHHHHHHCCCCEEEEHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHE
KLMVALGAAAHPQELREWFAE
EEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]