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The map label for this gene is nadB [C]

Identifier: 159898249

GI number: 159898249

Start: 2008940

End: 2010595

Strand: Direct

Name: nadB [C]

Synonym: Haur_1725

Alternate gene names: 159898249

Gene position: 2008940-2010595 (Clockwise)

Preceding gene: 159898247

Following gene: 159898253

Centisome position: 31.65

GC content: 52.9

Gene sequence:

>1656_bases
ATGCACTATGATGCCGATGTGGTCATTGTTGGAGCAGGTTTGGCAGGGCTGGTGGCCGCTGCTGAGCTAGCCGATGCCGG
CAAAAAGGTGATCTTGGTTGATCAAGAATCCGAGCAAAATCTTGGTGGTCAAGCATTTTGGTCGTTTGGTGGTTTATTTC
TCGTCGATTCCCCAGAACAACGTCGCCTCAAAATCAAAGATTCCTATGAATTAGCGTTACAAGATTGGCTAGGAACTGCC
GCGTTTGATCGGCCTGAAGATTATTGGCCGCGCAAGTGGGCTGAGGCGTATTTGGCCTTTGCTGCTGGCGAAAAACGCCA
ATGGCTCTATGATCAAGGGATGCGCTTTTTTCCTGTGGTTGGCTGGGCTGAACGTGGTGGCTATGGGGCAATTGGCCATG
GCAATTCGGTGCCACGCTTTCATATTACGTGGGGCACTGGCCCAGGTGTCGTTGCACCATTCGAGCGCCGCGTGCGAGCT
GCCGCCCAAAAAGGTTTGATCACGCTTAAATTTCGCCATCGGGTCAATCAATTATTGCTCAATGGCGGGGCAATCGCGGG
CATCGAGGGCGATATTTTGGAGCCAAGCAGCCTGCCACGCGGTGCACCAAGCTCGCGCAAGGTCGTCGGCAGCTTCACAT
TTCATGCACAAGCCGTAATTGTAACTTCAGGCGGTATCGGCGCAAACCACGATTTAGTACGCAAAAATTGGCCTGAACGG
CTGGGCCAAGCTCCCAAAACCATGGTTGCTGGTGTGCCCGATTATGTTGATGGCCGCATGCTGGCAATTACCGAGGCTGC
TGGTGGCACGATTATCAACCCTGATCGGATGTGGCATTATACCGAGGGCTTGCAAAATTGGAAGCCAATCTGGACTAACC
ATGGGATTCGGATTTTGCCCGGGCCATCGTCGATGTGGTTCGATGCGCTTGGTCAGCGATTACCAGTGCCACTTTTCCCT
GGCTTCGATAGCCTTGGCACACTCAAACATTTGATGCACACTGGCTACGACTATAGCTGGTTTATTCTGACTCAAAAAAT
TATCGAAAAAGAATTTGCGCTCTCTGGATCGGAGCAAAACCCCGATTTGACCAACAAAAGCGTACGCCAAGTGCTCGGTC
GGGTCTTACCGGGCGCAACTCCGCCAGTCGAAGCATTCAAACGCCATGGAGCCGATTTTGTTGTGCGCGATAATTTGGCC
GATTTGATCAAAGGTATGAATGCCCTGACCGCAGAGCCATTGCTGGACGCGGCGGCGATTGAGCGCGAAATTCAGGCACG
CGACCGCGAAATGACCAACCCATTTACCAAAGATATGCAAATTACGGCTTTGCGCGGTGCTCGTAACTACATCGGCGACA
AATTGATTCGGGTTGCCAAACCGCACAAAATTTTAGATCGCAGCGCTGGGCCATTGATTGCGGTGCGTTTGAATATTCTG
ACCCGCAAAAGCTTAGGTGGCCTGCAAACCGATCTTTCGGCACGGGTGATTGGCCAAGATGGACAGCCGATCAGCGGTTT
ATATGCTGCTGGTGAGGTAGCAGGCTTTGGCGGCGGTGGCATGCATGGCTATCGTTCGCTCGAAGGCACTTTCCTTGGGG
GCTGCATTTTCTCTGGGCGCACCGCCGGAAGGGCAGCGGCTGAGGCTTTAGCTTAA

Upstream 100 bases:

>100_bases
CCATTCACTCAAACCGCGAAGCTTGGCCAAATTTGAGCATAGTAGTGCCCAAAATCGCGTATAATCAACCAAATACCTAA
TGCAGCAGAATGGAGCACCC

Downstream 100 bases:

>100_bases
TTATTTGAGCGGCTTCGATCAAAAACTTGAAGCCGCTCAAATTCAGCTGATCATTTAAAGCACATTAATCACTATTTTAC
CCTGAGCATGGCCAGCCCCA

Product: putative FAD-binding dehydrogenase

Products: oxaloacetate; NH3; H2O2; iminoaspartate [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MHYDADVVIVGAGLAGLVAAAELADAGKKVILVDQESEQNLGGQAFWSFGGLFLVDSPEQRRLKIKDSYELALQDWLGTA
AFDRPEDYWPRKWAEAYLAFAAGEKRQWLYDQGMRFFPVVGWAERGGYGAIGHGNSVPRFHITWGTGPGVVAPFERRVRA
AAQKGLITLKFRHRVNQLLLNGGAIAGIEGDILEPSSLPRGAPSSRKVVGSFTFHAQAVIVTSGGIGANHDLVRKNWPER
LGQAPKTMVAGVPDYVDGRMLAITEAAGGTIINPDRMWHYTEGLQNWKPIWTNHGIRILPGPSSMWFDALGQRLPVPLFP
GFDSLGTLKHLMHTGYDYSWFILTQKIIEKEFALSGSEQNPDLTNKSVRQVLGRVLPGATPPVEAFKRHGADFVVRDNLA
DLIKGMNALTAEPLLDAAAIEREIQARDREMTNPFTKDMQITALRGARNYIGDKLIRVAKPHKILDRSAGPLIAVRLNIL
TRKSLGGLQTDLSARVIGQDGQPISGLYAAGEVAGFGGGGMHGYRSLEGTFLGGCIFSGRTAGRAAAEALA

Sequences:

>Translated_551_residues
MHYDADVVIVGAGLAGLVAAAELADAGKKVILVDQESEQNLGGQAFWSFGGLFLVDSPEQRRLKIKDSYELALQDWLGTA
AFDRPEDYWPRKWAEAYLAFAAGEKRQWLYDQGMRFFPVVGWAERGGYGAIGHGNSVPRFHITWGTGPGVVAPFERRVRA
AAQKGLITLKFRHRVNQLLLNGGAIAGIEGDILEPSSLPRGAPSSRKVVGSFTFHAQAVIVTSGGIGANHDLVRKNWPER
LGQAPKTMVAGVPDYVDGRMLAITEAAGGTIINPDRMWHYTEGLQNWKPIWTNHGIRILPGPSSMWFDALGQRLPVPLFP
GFDSLGTLKHLMHTGYDYSWFILTQKIIEKEFALSGSEQNPDLTNKSVRQVLGRVLPGATPPVEAFKRHGADFVVRDNLA
DLIKGMNALTAEPLLDAAAIEREIQARDREMTNPFTKDMQITALRGARNYIGDKLIRVAKPHKILDRSAGPLIAVRLNIL
TRKSLGGLQTDLSARVIGQDGQPISGLYAAGEVAGFGGGGMHGYRSLEGTFLGGCIFSGRTAGRAAAEALA
>Mature_551_residues
MHYDADVVIVGAGLAGLVAAAELADAGKKVILVDQESEQNLGGQAFWSFGGLFLVDSPEQRRLKIKDSYELALQDWLGTA
AFDRPEDYWPRKWAEAYLAFAAGEKRQWLYDQGMRFFPVVGWAERGGYGAIGHGNSVPRFHITWGTGPGVVAPFERRVRA
AAQKGLITLKFRHRVNQLLLNGGAIAGIEGDILEPSSLPRGAPSSRKVVGSFTFHAQAVIVTSGGIGANHDLVRKNWPER
LGQAPKTMVAGVPDYVDGRMLAITEAAGGTIINPDRMWHYTEGLQNWKPIWTNHGIRILPGPSSMWFDALGQRLPVPLFP
GFDSLGTLKHLMHTGYDYSWFILTQKIIEKEFALSGSEQNPDLTNKSVRQVLGRVLPGATPPVEAFKRHGADFVVRDNLA
DLIKGMNALTAEPLLDAAAIEREIQARDREMTNPFTKDMQITALRGARNYIGDKLIRVAKPHKILDRSAGPLIAVRLNIL
TRKSLGGLQTDLSARVIGQDGQPISGLYAAGEVAGFGGGGMHGYRSLEGTFLGGCIFSGRTAGRAAAEALA

Specific function: Able to catalyze the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids, but the ketosteroid dehydrogenase activity is low compared to KsdD in the cholesterol degradation process. The low activ

COG id: COG3573

COG function: function code R; Predicted oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.4.3.16 [C]

Molecular weight: Translated: 59863; Mature: 59863

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYDADVVIVGAGLAGLVAAAELADAGKKVILVDQESEQNLGGQAFWSFGGLFLVDSPEQ
CCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCCEEEEECCCC
RRLKIKDSYELALQDWLGTAAFDRPEDYWPRKWAEAYLAFAAGEKRQWLYDQGMRFFPVV
CEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEEE
GWAERGGYGAIGHGNSVPRFHITWGTGPGVVAPFERRVRAAAQKGLITLKFRHRVNQLLL
EECCCCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHH
NGGAIAGIEGDILEPSSLPRGAPSSRKVVGSFTFHAQAVIVTSGGIGANHDLVRKNWPER
CCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCHHHCCCHHH
LGQAPKTMVAGVPDYVDGRMLAITEAAGGTIINPDRMWHYTEGLQNWKPIWTNHGIRILP
HCCCCHHHHCCCCCHHCCEEEEEEECCCCCEECCCHHHHHHHHHCCCCCEECCCCEEEEE
GPSSMWFDALGQRLPVPLFPGFDSLGTLKHLMHTGYDYSWFILTQKIIEKEFALSGSEQN
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCC
PDLTNKSVRQVLGRVLPGATPPVEAFKRHGADFVVRDNLADLIKGMNALTAEPLLDAAAI
CCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCHHHHHHCCHHHHHHHHHHHHHH
EREIQARDREMTNPFTKDMQITALRGARNYIGDKLIRVAKPHKILDRSAGPLIAVRLNIL
HHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEH
TRKSLGGLQTDLSARVIGQDGQPISGLYAAGEVAGFGGGGMHGYRSLEGTFLGGCIFSGR
HHHHCCCCCCCCEEEEECCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
TAGRAAAEALA
CCCHHHHHHCC
>Mature Secondary Structure
MHYDADVVIVGAGLAGLVAAAELADAGKKVILVDQESEQNLGGQAFWSFGGLFLVDSPEQ
CCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCCEEEEECCCC
RRLKIKDSYELALQDWLGTAAFDRPEDYWPRKWAEAYLAFAAGEKRQWLYDQGMRFFPVV
CEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCEEEEEE
GWAERGGYGAIGHGNSVPRFHITWGTGPGVVAPFERRVRAAAQKGLITLKFRHRVNQLLL
EECCCCCEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHH
NGGAIAGIEGDILEPSSLPRGAPSSRKVVGSFTFHAQAVIVTSGGIGANHDLVRKNWPER
CCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCCCCCHHHCCCHHH
LGQAPKTMVAGVPDYVDGRMLAITEAAGGTIINPDRMWHYTEGLQNWKPIWTNHGIRILP
HCCCCHHHHCCCCCHHCCEEEEEEECCCCCEECCCHHHHHHHHHCCCCCEECCCCEEEEE
GPSSMWFDALGQRLPVPLFPGFDSLGTLKHLMHTGYDYSWFILTQKIIEKEFALSGSEQN
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCC
PDLTNKSVRQVLGRVLPGATPPVEAFKRHGADFVVRDNLADLIKGMNALTAEPLLDAAAI
CCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEECCHHHHHHCCHHHHHHHHHHHHHH
EREIQARDREMTNPFTKDMQITALRGARNYIGDKLIRVAKPHKILDRSAGPLIAVRLNIL
HHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEH
TRKSLGGLQTDLSARVIGQDGQPISGLYAAGEVAGFGGGGMHGYRSLEGTFLGGCIFSGR
HHHHCCCCCCCCEEEEECCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
TAGRAAAEALA
CCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: L-Aspartate [C]

Kcat value (1/min): 2 [C]

Specific activity: NA

Km value (mM): 4.1 {L-aspartate}} 0.5 {L-aspartate}} [C]

Substrates: @ASPA02txt*L-Aspartate!; H2O; O2; L-aspartate [C]

Specific reaction: @ASOA02.txt*L-Aspartate! + H2O + O2 = oxaloacetate + NH3 + H2O2 O2 + L-aspartate = H2O2 + iminoaspartate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: Iodoacetate; L-Aspartate; Mesotartrate; NAD+; Tetranitromethane; Urea [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA