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The map label for this gene is nfo

Identifier: 159898151

GI number: 159898151

Start: 1886872

End: 1887753

Strand: Direct

Name: nfo

Synonym: Haur_1627

Alternate gene names: 159898151

Gene position: 1886872-1887753 (Clockwise)

Preceding gene: 159898145

Following gene: 159898152

Centisome position: 29.73

GC content: 51.93

Gene sequence:

>882_bases
ATGCCCAATTTAGGAGCGCATGTTTCAACCGTTGGTGGGTTGCAGACCGCCTTTGAGCGAGCGCAAGCCACGACCTGTCA
TGTGATTCAAATTTTTACCAAAAGCCAACGCCAATGGAATGCCAAACCGCTGACTGCTGCCGATTGTGCCAATTTTCAAG
CGGCCCATCGTGACGCTGGCATGCCACCCTTGATTGCCCACGATTCCTATTTGATCAACTTGGGCAGCCCCGACAATGGC
TTATGGGAAAAATCGATTGCAGCCTTTCGCGTTGAGCTAGAGCGTTGTCAGCAATTGGGAGTTGGCTCGTTGGTGACCCA
TCCTGGGGCGCATGTTGGCTCAGGCGAGGCCGCTGGCCTTGATCGGGTTGGCGCAGCCTTGCGGCGTTTACTGGCCGAAG
ACGTTGGCGGCGAAACCCAGATTTTGCTCGAAATTACTGCTGGACAAGGCACGGCCTTAGGTCATAGTTTCGAGCATTTG
GCTCGCTTGATCGAGCTGTGCGATGGTCATCCACGGCTTGGAATTTGCTTTGATACCTGCCATGGCTTAGCTGCTGGCTA
CGATTTTCGCACTGCTGAAGGCTACCAAACCACCTTTGATCATTTTGATCGTTTAATTGGGATTGATCGGCTGCGGGCGT
TTCATCTTAACGATTCGAAAAACGATCTTGGCAGTCGGGTTGATCGGCATACCCATATTGGTGAGGGATTTGTGGGCTTA
GAAGGGTTTGAACTCATTATGAACGATCCTCGCTTTCAAACGATTCCGATGGCCTTAGAGACTCCCAAAGAACCCGATGA
AAGCGCAGATATTCGCAATTTGGCAACGTTGCGCGGTTTGCGCCGCGCAAGCGTTAGTGCATCACCACAAAGCGAGGTAT
AA

Upstream 100 bases:

>100_bases
CAAAATAAAAATCATCATGAACTATTACCCACGCCTAATCTCAACAACGGCTTGAAAGTGCTATAATTGCAGGCGACACT
TGTTTGGAGTGAGAAACCCC

Downstream 100 bases:

>100_bases
ATGGGAAACCAACCAAAAAGCAAACGCAAGTTGTGGGTATCGTTATTGTTGGGCTTGGCCGCTGTGGTGGTGATTGTGCG
GCGCAAGCTGCATCCGATCG

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPNLGAHVSTVGGLQTAFERAQATTCHVIQIFTKSQRQWNAKPLTAADCANFQAAHRDAGMPPLIAHDSYLINLGSPDNG
LWEKSIAAFRVELERCQQLGVGSLVTHPGAHVGSGEAAGLDRVGAALRRLLAEDVGGETQILLEITAGQGTALGHSFEHL
ARLIELCDGHPRLGICFDTCHGLAAGYDFRTAEGYQTTFDHFDRLIGIDRLRAFHLNDSKNDLGSRVDRHTHIGEGFVGL
EGFELIMNDPRFQTIPMALETPKEPDESADIRNLATLRGLRRASVSASPQSEV

Sequences:

>Translated_293_residues
MPNLGAHVSTVGGLQTAFERAQATTCHVIQIFTKSQRQWNAKPLTAADCANFQAAHRDAGMPPLIAHDSYLINLGSPDNG
LWEKSIAAFRVELERCQQLGVGSLVTHPGAHVGSGEAAGLDRVGAALRRLLAEDVGGETQILLEITAGQGTALGHSFEHL
ARLIELCDGHPRLGICFDTCHGLAAGYDFRTAEGYQTTFDHFDRLIGIDRLRAFHLNDSKNDLGSRVDRHTHIGEGFVGL
EGFELIMNDPRFQTIPMALETPKEPDESADIRNLATLRGLRRASVSASPQSEV
>Mature_292_residues
PNLGAHVSTVGGLQTAFERAQATTCHVIQIFTKSQRQWNAKPLTAADCANFQAAHRDAGMPPLIAHDSYLINLGSPDNGL
WEKSIAAFRVELERCQQLGVGSLVTHPGAHVGSGEAAGLDRVGAALRRLLAEDVGGETQILLEITAGQGTALGHSFEHLA
RLIELCDGHPRLGICFDTCHGLAAGYDFRTAEGYQTTFDHFDRLIGIDRLRAFHLNDSKNDLGSRVDRHTHIGEGFVGLE
GFELIMNDPRFQTIPMALETPKEPDESADIRNLATLRGLRRASVSASPQSEV

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=269, Percent_Identity=44.9814126394052, Blast_Score=214, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17531193, Length=276, Percent_Identity=40.9420289855072, Blast_Score=213, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6322735, Length=267, Percent_Identity=35.9550561797753, Blast_Score=182, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_HERA2 (A9B5N7)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001544398.1
- ProteinModelPortal:   A9B5N7
- SMR:   A9B5N7
- GeneID:   5733499
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_1627
- HOGENOM:   HBG565018
- OMA:   QIALETM
- BioCyc:   HAUR316274:HAUR_1627-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31751; Mature: 31620

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLGAHVSTVGGLQTAFERAQATTCHVIQIFTKSQRQWNAKPLTAADCANFQAAHRDAG
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHEEHHHHHHCCCCCCCHHHHHCHHHHHHCCC
MPPLIAHDSYLINLGSPDNGLWEKSIAAFRVELERCQQLGVGSLVTHPGAHVGSGEAAGL
CCCEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
DRVGAALRRLLAEDVGGETQILLEITAGQGTALGHSFEHLARLIELCDGHPRLGICFDTC
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHH
HGLAAGYDFRTAEGYQTTFDHFDRLIGIDRLRAFHLNDSKNDLGSRVDRHTHIGEGFVGL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCC
EGFELIMNDPRFQTIPMALETPKEPDESADIRNLATLRGLRRASVSASPQSEV
CCHHEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PNLGAHVSTVGGLQTAFERAQATTCHVIQIFTKSQRQWNAKPLTAADCANFQAAHRDAG
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHEEHHHHHHCCCCCCCHHHHHCHHHHHHCCC
MPPLIAHDSYLINLGSPDNGLWEKSIAAFRVELERCQQLGVGSLVTHPGAHVGSGEAAGL
CCCEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
DRVGAALRRLLAEDVGGETQILLEITAGQGTALGHSFEHLARLIELCDGHPRLGICFDTC
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHH
HGLAAGYDFRTAEGYQTTFDHFDRLIGIDRLRAFHLNDSKNDLGSRVDRHTHIGEGFVGL
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCC
EGFELIMNDPRFQTIPMALETPKEPDESADIRNLATLRGLRRASVSASPQSEV
CCHHEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA