Click here to switch to the map view.

The map label for this gene is ddeIM [H]

Identifier: 159897859

GI number: 159897859

Start: 1543504

End: 1544751

Strand: Direct

Name: ddeIM [H]

Synonym: Haur_1333

Alternate gene names: 159897859

Gene position: 1543504-1544751 (Clockwise)

Preceding gene: 159897858

Following gene: 159897862

Centisome position: 24.32

GC content: 38.14

Gene sequence:

>1248_bases
ATGCCGATTACCCTTAAGACAATAGAATTATTTGCTGGAGCTGGAGGCCTCGGGCTAGGGTTTCTTCTCGCAAATCATCC
AGGTGTCAATTTTAGGCCTTTATGTGCAGTCGATTTTAACGTAGACGCATGTACTAGCTATAATATGAATATGCAATGGC
TGCATCAGAATGCTCCTCATTTACAGACAACACAAGCTTCTAAGGCTTATCTGCGGAAAGTTGAATCCTTAAACGTTAAT
GCAGTGAAGAGGCTTTTCCAGTTACAACAAGGTGATCTCGATATTTTAATGGGTGGTCCTCCTTGTCAAGGATATTCATC
TTCAAATCGCCAGGCATCAAAAGAAACACGCGATGAACTTAATAATATGGTGAAATCCTTTCTTGATCGAGTTCAAGATT
TTTCACCAAAAATGTTTCTCTTAGAAAATGTCCAAGGAGTCACATGGACTGCCTCGACTGACGAAATGAGAATACCTAGT
GAGCAATTATCCTTTATAGATAATGAAGAGATTGCTGATGTTAAAGACTATTTAGTTCATAGAGCACGCGAGCTGGGTTA
TCACATATGGTATTCGGTGCTTGATGCAGCGGATTTTGGTGTCCCTCAACATAGAAAACGATTTTTTCTTTTTGGTATTC
GTACAGACTTGACAACTGACCCAAATATTCGGCTTGAAAAATTTATCAATCCTTATAGAACGAGCACACTTACAACAGTT
GCCCAAGCTATTGAGGATCTTCCTGTTATTAATAATGGCGAGCATTGGAAAGGTAATAACTATAATCCGGTGGCGAATGG
GTATATCACTATGATGCGTAGCTTTATGAATAATAATGTTTTATTTGACCACTTTACAACAAATCATCAAGAATATGTTC
TTGAGCGTTTCAGAAATATTCCTGAAGGCGAAAATTGGAAATCTATAAAAAATATTATGAATACGTATAAAAATGTAAAC
AAAACCCATAGTAATATTTATAGAAGATTACAACGGAATGCCCCATCGCATACTATTAGTCATTACCGCAAAGCAATGAC
TATCCATCCTGTACAGAATAGAGGATTATCATTTAGAGAAGCCTGTAGATTGCAGTCTTTTCCAGACTGGTATCGATTTA
GTGGAACAAGAGAAAGTGCCCAACAGCAACTAGCGAATGCAGTGCCACCTTTGCTTTCGTCAAAGGTGGCACTGGCTATC
GCAGATTATTGGTTATCTCTGCCACATAATGCTCTTATGAAAGATTAA

Upstream 100 bases:

>100_bases
ACTCTGGTATATTATGATGCCTATTTCTGCTTAATTTTTTATTAATTATTTACACTTAGTAATGTAGTCTCATGTAAGTA
ATTAAGACTGCTGTATTTCT

Downstream 100 bases:

>100_bases
AGACAAAAGTATATTTGTCTTTTGTATCTGGAAATGTTATTGCAGGTATCCAAAAAGGTTTTCCATTCCAGTTTTCGCCA
GTAACTTTGAATATAAATAA

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.DdeI; Cytosine-specific methyltransferase DdeI [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MPITLKTIELFAGAGGLGLGFLLANHPGVNFRPLCAVDFNVDACTSYNMNMQWLHQNAPHLQTTQASKAYLRKVESLNVN
AVKRLFQLQQGDLDILMGGPPCQGYSSSNRQASKETRDELNNMVKSFLDRVQDFSPKMFLLENVQGVTWTASTDEMRIPS
EQLSFIDNEEIADVKDYLVHRARELGYHIWYSVLDAADFGVPQHRKRFFLFGIRTDLTTDPNIRLEKFINPYRTSTLTTV
AQAIEDLPVINNGEHWKGNNYNPVANGYITMMRSFMNNNVLFDHFTTNHQEYVLERFRNIPEGENWKSIKNIMNTYKNVN
KTHSNIYRRLQRNAPSHTISHYRKAMTIHPVQNRGLSFREACRLQSFPDWYRFSGTRESAQQQLANAVPPLLSSKVALAI
ADYWLSLPHNALMKD

Sequences:

>Translated_415_residues
MPITLKTIELFAGAGGLGLGFLLANHPGVNFRPLCAVDFNVDACTSYNMNMQWLHQNAPHLQTTQASKAYLRKVESLNVN
AVKRLFQLQQGDLDILMGGPPCQGYSSSNRQASKETRDELNNMVKSFLDRVQDFSPKMFLLENVQGVTWTASTDEMRIPS
EQLSFIDNEEIADVKDYLVHRARELGYHIWYSVLDAADFGVPQHRKRFFLFGIRTDLTTDPNIRLEKFINPYRTSTLTTV
AQAIEDLPVINNGEHWKGNNYNPVANGYITMMRSFMNNNVLFDHFTTNHQEYVLERFRNIPEGENWKSIKNIMNTYKNVN
KTHSNIYRRLQRNAPSHTISHYRKAMTIHPVQNRGLSFREACRLQSFPDWYRFSGTRESAQQQLANAVPPLLSSKVALAI
ADYWLSLPHNALMKD
>Mature_414_residues
PITLKTIELFAGAGGLGLGFLLANHPGVNFRPLCAVDFNVDACTSYNMNMQWLHQNAPHLQTTQASKAYLRKVESLNVNA
VKRLFQLQQGDLDILMGGPPCQGYSSSNRQASKETRDELNNMVKSFLDRVQDFSPKMFLLENVQGVTWTASTDEMRIPSE
QLSFIDNEEIADVKDYLVHRARELGYHIWYSVLDAADFGVPQHRKRFFLFGIRTDLTTDPNIRLEKFINPYRTSTLTTVA
QAIEDLPVINNGEHWKGNNYNPVANGYITMMRSFMNNNVLFDHFTTNHQEYVLERFRNIPEGENWKSIKNIMNTYKNVNK
THSNIYRRLQRNAPSHTISHYRKAMTIHPVQNRGLSFREACRLQSFPDWYRFSGTRESAQQQLANAVPPLLSSKVALAIA
DYWLSLPHNALMKD

Specific function: This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=336, Percent_Identity=26.7857142857143, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI4503351, Length=336, Percent_Identity=26.7857142857143, Blast_Score=96, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 47654; Mature: 47522

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITLKTIELFAGAGGLGLGFLLANHPGVNFRPLCAVDFNVDACTSYNMNMQWLHQNAPH
CCCEEEEHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCC
LQTTQASKAYLRKVESLNVNAVKRLFQLQQGDLDILMGGPPCQGYSSSNRQASKETRDEL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
NNMVKSFLDRVQDFSPKMFLLENVQGVTWTASTDEMRIPSEQLSFIDNEEIADVKDYLVH
HHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHH
RARELGYHIWYSVLDAADFGVPQHRKRFFLFGIRTDLTTDPNIRLEKFINPYRTSTLTTV
HHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCCCCCCHHHHHCCHHHHHHHHHH
AQAIEDLPVINNGEHWKGNNYNPVANGYITMMRSFMNNNVLFDHFTTNHQEYVLERFRNI
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCC
PEGENWKSIKNIMNTYKNVNKTHSNIYRRLQRNAPSHTISHYRKAMTIHPVQNRGLSFRE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCHHCCCCCHHH
ACRLQSFPDWYRFSGTRESAQQQLANAVPPLLSSKVALAIADYWLSLPHNALMKD
HHHHCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
PITLKTIELFAGAGGLGLGFLLANHPGVNFRPLCAVDFNVDACTSYNMNMQWLHQNAPH
CCEEEEHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCC
LQTTQASKAYLRKVESLNVNAVKRLFQLQQGDLDILMGGPPCQGYSSSNRQASKETRDEL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
NNMVKSFLDRVQDFSPKMFLLENVQGVTWTASTDEMRIPSEQLSFIDNEEIADVKDYLVH
HHHHHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHH
RARELGYHIWYSVLDAADFGVPQHRKRFFLFGIRTDLTTDPNIRLEKFINPYRTSTLTTV
HHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCCCCCCHHHHHCCHHHHHHHHHH
AQAIEDLPVINNGEHWKGNNYNPVANGYITMMRSFMNNNVLFDHFTTNHQEYVLERFRNI
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCC
PEGENWKSIKNIMNTYKNVNKTHSNIYRRLQRNAPSHTISHYRKAMTIHPVQNRGLSFRE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCHHCCCCCHHH
ACRLQSFPDWYRFSGTRESAQQQLANAVPPLLSSKVALAIADYWLSLPHNALMKD
HHHHCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2823226 [H]