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The map label for this gene is yugF [H]

Identifier: 159897788

GI number: 159897788

Start: 1469420

End: 1470274

Strand: Direct

Name: yugF [H]

Synonym: Haur_1259

Alternate gene names: 159897788

Gene position: 1469420-1470274 (Clockwise)

Preceding gene: 159897787

Following gene: 159897789

Centisome position: 23.15

GC content: 51.93

Gene sequence:

>855_bases
ATGCCAATCTATACCGTCAACGATCAATCGATTCATGTGTGGGAAGAGGGTGCAAGCGATAAACAAGTAGCCTTGTTAAT
TCATGGCTGGTCAAGCTCATGGTTTGCCATGTCGCCGTTGTTTCCCATTTTGTCGCGCTATCGCTGTTTGGCGGTCGATT
TGCCAGGCTACGGCCAATCGCCTAAGGGTGATGATCCGGTCAGCATTGTGGCCTATACCGATCTGTTGGCCGACCTTTTG
CGCCAAGTCACCGATCAACCGGCAGTGTTGATTGGCCATTCGATGGGTGGCATGATTAGCTTGACCATGAGTTTGCGTCA
CCCTGAGCTAATCGAGCGCATGGTCTTAATTTGCCCAACGATCAGCGGGCGTTTATCGATGTTTATCAATATGTTTCTTG
GGCCGTTGGTAGTGTTGGAGCGCGTGCCTTTTGCCGATCGAGTAACTTCGCTGATTGAAACGCGTATCTGGCGAATTACC
GACCGCGTGATGAAACCAGCCTCGTATGCCGAGCGCAGCGCCATTAGTAGCGAAGCCCATGAACGTATCAAGGCCGATGT
GCGGCGGCCAGGTCAAGGCCAAATTCGCGCTGAATGCTACCGTGCCATGCGCGAAAACGATTTGCGTGGGCAATTGCAAA
AAATTAGCCATCCTTCGCTCTATTTATGGGGCATGGAAGATAACACAGTGCCCTTGCGCGATGCCAGCAGCGTCGTCGCC
GAATTGCCCGATGCCGATTTGCGGATTATTCCCAACGCTGGGCATTGGCCCCATTTTGAAGCCCTCGTTACCACTCAACG
CTATGTTCGGGCATTTCTTAGCACCCCGATTAAGTTGCTCAAGGCACAATTTTAG

Upstream 100 bases:

>100_bases
ATTCTTTCGTAATTTGCAAGAAAAAGCCCGTGAAGCCCGTCAACGCCGTGATCGCCGCCCGCCTGACCAAACCAGCTAAC
CACCAGCGGAGGCTCCAGCT

Downstream 100 bases:

>100_bases
CTGTGGAGTTGGTTTATGGATAGTTCAGCCGAACCAAGTGGCGGCCTTAGTTCCTTAGATTTTGAGCATTTATCGTTGGC
CGAGCGCACCGTGATTCGGG

Product: alpha/beta hydrolase fold-domain containing protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPIYTVNDQSIHVWEEGASDKQVALLIHGWSSSWFAMSPLFPILSRYRCLAVDLPGYGQSPKGDDPVSIVAYTDLLADLL
RQVTDQPAVLIGHSMGGMISLTMSLRHPELIERMVLICPTISGRLSMFINMFLGPLVVLERVPFADRVTSLIETRIWRIT
DRVMKPASYAERSAISSEAHERIKADVRRPGQGQIRAECYRAMRENDLRGQLQKISHPSLYLWGMEDNTVPLRDASSVVA
ELPDADLRIIPNAGHWPHFEALVTTQRYVRAFLSTPIKLLKAQF

Sequences:

>Translated_284_residues
MPIYTVNDQSIHVWEEGASDKQVALLIHGWSSSWFAMSPLFPILSRYRCLAVDLPGYGQSPKGDDPVSIVAYTDLLADLL
RQVTDQPAVLIGHSMGGMISLTMSLRHPELIERMVLICPTISGRLSMFINMFLGPLVVLERVPFADRVTSLIETRIWRIT
DRVMKPASYAERSAISSEAHERIKADVRRPGQGQIRAECYRAMRENDLRGQLQKISHPSLYLWGMEDNTVPLRDASSVVA
ELPDADLRIIPNAGHWPHFEALVTTQRYVRAFLSTPIKLLKAQF
>Mature_283_residues
PIYTVNDQSIHVWEEGASDKQVALLIHGWSSSWFAMSPLFPILSRYRCLAVDLPGYGQSPKGDDPVSIVAYTDLLADLLR
QVTDQPAVLIGHSMGGMISLTMSLRHPELIERMVLICPTISGRLSMFINMFLGPLVVLERVPFADRVTSLIETRIWRITD
RVMKPASYAERSAISSEAHERIKADVRRPGQGQIRAECYRAMRENDLRGQLQKISHPSLYLWGMEDNTVPLRDASSVVAE
LPDADLRIIPNAGHWPHFEALVTTQRYVRAFLSTPIKLLKAQF

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=283, Percent_Identity=26.1484098939929, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17554012, Length=278, Percent_Identity=21.9424460431655, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 32022; Mature: 31890

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIYTVNDQSIHVWEEGASDKQVALLIHGWSSSWFAMSPLFPILSRYRCLAVDLPGYGQS
CCEEECCCCEEEEEECCCCCCEEEEEEECCCCCCEEHHHHHHHHHCCEEEEEECCCCCCC
PKGDDPVSIVAYTDLLADLLRQVTDQPAVLIGHSMGGMISLTMSLRHPELIERMVLICPT
CCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHCCC
ISGRLSMFINMFLGPLVVLERVPFADRVTSLIETRIWRITDRVMKPASYAERSAISSEAH
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ERIKADVRRPGQGQIRAECYRAMRENDLRGQLQKISHPSLYLWGMEDNTVPLRDASSVVA
HHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
ELPDADLRIIPNAGHWPHFEALVTTQRYVRAFLSTPIKLLKAQF
HCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCC
>Mature Secondary Structure 
PIYTVNDQSIHVWEEGASDKQVALLIHGWSSSWFAMSPLFPILSRYRCLAVDLPGYGQS
CEEECCCCEEEEEECCCCCCEEEEEEECCCCCCEEHHHHHHHHHCCEEEEEECCCCCCC
PKGDDPVSIVAYTDLLADLLRQVTDQPAVLIGHSMGGMISLTMSLRHPELIERMVLICPT
CCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHCCC
ISGRLSMFINMFLGPLVVLERVPFADRVTSLIETRIWRITDRVMKPASYAERSAISSEAH
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ERIKADVRRPGQGQIRAECYRAMRENDLRGQLQKISHPSLYLWGMEDNTVPLRDASSVVA
HHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHH
ELPDADLRIIPNAGHWPHFEALVTTQRYVRAFLSTPIKLLKAQF
HCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]