Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 159897768

GI number: 159897768

Start: 1443142

End: 1444236

Strand: Reverse

Name: rfbB [H]

Synonym: Haur_1239

Alternate gene names: 159897768

Gene position: 1444236-1443142 (Counterclockwise)

Preceding gene: 159897769

Following gene: 159897767

Centisome position: 22.76

GC content: 49.86

Gene sequence:

>1095_bases
ATGCGCATTGCAGTTACCGGCGGGGCAGGCTTTATTGGCTCCAACTTTGTGCGATATTGGATGGATACAAATCCTGCTGA
TGAGGTTGTGGTGATTGATGCCTTGACCTATGCTGGCCATCTGAGTAATTTGGCAGGCTACCACGAACGCGCCAATTGCC
AATTTGTGCAGGCTGATATTTGCGATTACCCAACCATGCTCAAGGTATTGGCGGGGGTCAATTTGGTAGTACACTTTGCC
GCCGAAACCCACGTTGATCGCTCGTTGGGTGAGTTTGAAATGGAGCGTCAATTTTATCGCTCGAATATCGAAGGTACTGC
CAGCTTACTACGAGCTTCCCGCGAGGCTGGAGTTGGGCATTTTCACCATGTTTCGACCGATGAAGTGTTTGGCGATTTAG
ACTTTGATGATCCGCAAAAATTCCACGAAACCTATCCCTATAATCCCAGCAGCCCTTATGCGGTCAGCAAAGCAGCCTCG
GATCATGTGGTGCGAGCCTTTGCCCACACCCACAAATACCCAATCACCATCACCAACTGCACCAACAATTATGGCCCATT
CCAAACTCCTGAAAAGCTGATTCCCCGTTCAATTGCCTTGTTACTGGCCGGCCAAAAAGTGAAGCTCTATACTGATGCCG
AGGGCATTCCAGGCCGTAACATCCGCGATTGGTTGCATGTGCAAGATCACTGTGAAGCGATTGCTTTGGTGATTCAAAAA
GGTCGGATTGGTGAAACCTATGGGATTGGCGGCGAGGCCGAGCTTTCCAACTATCACTTGGTCGAAACCATGCTCGATAT
TATGAGCGAATATTTAGATCGCACGTTGACAATTGAAAATAGCGTCGAATTTGTGGCTGATCGCCCAGGCCACGACCGCC
GTTATGCCATGGATTTGAGCAAAATCAAGCGTGAGTTGGGCTGGCAGCCACGTTATTCGTTCCAACAAGGCTTTTTAGAA
ACCGTGCAATGGTACACATCGCCCGAAGGTCAAGCATGGCTGGCAAGCTTGAACGATCGTACCAGCGATGTCCGCGCCAA
CCAAGAGCAAGTCGTTGCTGTGCGCGAAAACTGGCAAGCTGAGCATCAATCCTAA

Upstream 100 bases:

>100_bases
GCGCACTCATTAGTTGACAACTACCCCGCTATCATCCACAATGCAGCGACTATTGACGACAATTCAGGCATTCCAATGAT
AGAAAGGTTCCAAGGTTTTT

Downstream 100 bases:

>100_bases
TTAGGGCATGTCCTCACCCCCAACCCCTCTCTTTGAAGGGTCGGTTATTAACAATGGCTAGATGGAATTCAACGTTGATT
AGCATACCCTCACCCCTAGC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MRIAVTGGAGFIGSNFVRYWMDTNPADEVVVIDALTYAGHLSNLAGYHERANCQFVQADICDYPTMLKVLAGVNLVVHFA
AETHVDRSLGEFEMERQFYRSNIEGTASLLRASREAGVGHFHHVSTDEVFGDLDFDDPQKFHETYPYNPSSPYAVSKAAS
DHVVRAFAHTHKYPITITNCTNNYGPFQTPEKLIPRSIALLLAGQKVKLYTDAEGIPGRNIRDWLHVQDHCEAIALVIQK
GRIGETYGIGGEAELSNYHLVETMLDIMSEYLDRTLTIENSVEFVADRPGHDRRYAMDLSKIKRELGWQPRYSFQQGFLE
TVQWYTSPEGQAWLASLNDRTSDVRANQEQVVAVRENWQAEHQS

Sequences:

>Translated_364_residues
MRIAVTGGAGFIGSNFVRYWMDTNPADEVVVIDALTYAGHLSNLAGYHERANCQFVQADICDYPTMLKVLAGVNLVVHFA
AETHVDRSLGEFEMERQFYRSNIEGTASLLRASREAGVGHFHHVSTDEVFGDLDFDDPQKFHETYPYNPSSPYAVSKAAS
DHVVRAFAHTHKYPITITNCTNNYGPFQTPEKLIPRSIALLLAGQKVKLYTDAEGIPGRNIRDWLHVQDHCEAIALVIQK
GRIGETYGIGGEAELSNYHLVETMLDIMSEYLDRTLTIENSVEFVADRPGHDRRYAMDLSKIKRELGWQPRYSFQQGFLE
TVQWYTSPEGQAWLASLNDRTSDVRANQEQVVAVRENWQAEHQS
>Mature_364_residues
MRIAVTGGAGFIGSNFVRYWMDTNPADEVVVIDALTYAGHLSNLAGYHERANCQFVQADICDYPTMLKVLAGVNLVVHFA
AETHVDRSLGEFEMERQFYRSNIEGTASLLRASREAGVGHFHHVSTDEVFGDLDFDDPQKFHETYPYNPSSPYAVSKAAS
DHVVRAFAHTHKYPITITNCTNNYGPFQTPEKLIPRSIALLLAGQKVKLYTDAEGIPGRNIRDWLHVQDHCEAIALVIQK
GRIGETYGIGGEAELSNYHLVETMLDIMSEYLDRTLTIENSVEFVADRPGHDRRYAMDLSKIKRELGWQPRYSFQQGFLE
TVQWYTSPEGQAWLASLNDRTSDVRANQEQVVAVRENWQAEHQS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=37.9204892966361, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=45.6090651558074, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788353, Length=345, Percent_Identity=45.2173913043478, Blast_Score=287, Evalue=6e-79,
Organism=Escherichia coli, GI1786974, Length=357, Percent_Identity=25.7703081232493, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=37.1601208459215, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI115532424, Length=325, Percent_Identity=32.3076923076923, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319493, Length=348, Percent_Identity=25.5747126436782, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19923002, Length=363, Percent_Identity=24.7933884297521, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=23.9644970414201, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41133; Mature: 41133

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVTGGAGFIGSNFVRYWMDTNPADEVVVIDALTYAGHLSNLAGYHERANCQFVQADI
CEEEEECCCCCHHCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEHHH
CDYPTMLKVLAGVNLVVHFAAETHVDRSLGEFEMERQFYRSNIEGTASLLRASREAGVGH
CCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
FHHVSTDEVFGDLDFDDPQKFHETYPYNPSSPYAVSKAASDHVVRAFAHTHKYPITITNC
EEECCHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEECC
TNNYGPFQTPEKLIPRSIALLLAGQKVKLYTDAEGIPGRNIRDWLHVQDHCEAIALVIQK
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GRIGETYGIGGEAELSNYHLVETMLDIMSEYLDRTLTIENSVEFVADRPGHDRRYAMDLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCEEECCCCCCHHHHHHHH
KIKRELGWQPRYSFQQGFLETVQWYTSPEGQAWLASLNDRTSDVRANQEQVVAVRENWQA
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHEEEEHHCCCC
EHQS
CCCC
>Mature Secondary Structure
MRIAVTGGAGFIGSNFVRYWMDTNPADEVVVIDALTYAGHLSNLAGYHERANCQFVQADI
CEEEEECCCCCHHCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCEEEEHHH
CDYPTMLKVLAGVNLVVHFAAETHVDRSLGEFEMERQFYRSNIEGTASLLRASREAGVGH
CCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
FHHVSTDEVFGDLDFDDPQKFHETYPYNPSSPYAVSKAASDHVVRAFAHTHKYPITITNC
EEECCHHHHCCCCCCCCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEECC
TNNYGPFQTPEKLIPRSIALLLAGQKVKLYTDAEGIPGRNIRDWLHVQDHCEAIALVIQK
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GRIGETYGIGGEAELSNYHLVETMLDIMSEYLDRTLTIENSVEFVADRPGHDRRYAMDLS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCEEECCCCCCHHHHHHHH
KIKRELGWQPRYSFQQGFLETVQWYTSPEGQAWLASLNDRTSDVRANQEQVVAVRENWQA
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHEEEEHHCCCC
EHQS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]