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The map label for this gene is gltX

Identifier: 159897286

GI number: 159897286

Start: 859688

End: 861175

Strand: Reverse

Name: gltX

Synonym: Haur_0757

Alternate gene names: 159897286

Gene position: 861175-859688 (Counterclockwise)

Preceding gene: 159897287

Following gene: 159897285

Centisome position: 13.57

GC content: 52.08

Gene sequence:

>1488_bases
ATGTCGGATCGTCCAACACCAGCCCGCACGCGCTTTGCGCCAAGTCCGACGGGCTATCTGCATATCGGTAGTTTACGTAC
CGTGTTATTTAGCTGGTTGTGGGCTCGCCACACTGGCGGCCAATTTTTATTACGGATTGAAGATACCGATCGCAAGCGCT
TTGTCGAAGGCGCAGAAGAGCAATTAACCAGTTCGTTGCAAGCGATCGGCCTAATGTGGGATGAAGGTCCAATTGTTGGC
GGTCCTCACGCACCCTATAAACAATCCGAACGCTTGGAAATTTACCAAGCTCACGCCCAAGCCTTGATCGATAAAGGTGT
GGCCTATCGTTCGTATGCTACTGCCGACGAAATTGCGGCGATCAATGCCGAACGTGAAGCTCGCGGCGAGCCAAAGCTCT
TGGTGTTTCGCAATTTGCCAGGCATCGACGATGCCGCTCGTGAAGCCGCAGGCGCTGACTATAACGTGCGGCTAAGCCTC
AAAACGACTGGCCAAACCGTCGTTCAGGATCTTGTGCGCGGCCAAATCGTCTTTGATAATGCTGCCTTGAAAATGCCCGA
CCCAGTGTTGTTGAAAACCGATGGCTTCCCAACCTATGCCCTCGCTGCCATGGTCGATGATCACTTGATGGGGATCACCC
ATGTGCTGCGGGCTGATGAGTGGATTCCAACCTGGCCAATTCATCATCAAATTTACGAGGCCTTCGGTTGGGAGCAACCA
GTTTGGGTGCATGTGCCGCAAGTCTTGGGCAGCGATGGCAAGAAGCTTTCCAAACGCCACGGCGATACATCGGTCACCGA
GTATATCGATTTAGGCTTTGTGCCCGAAGCCATTATCAACTATTTGGCCTTGATCGGCTGGTCATACGATGATAAAACAG
AGTTTATGACCCTTGAAGAGTTGATCGAGCGCTTTGATCTGAATCGGATTCGGCCATCGGGCGGGGTGTTTGATCGCGAT
AAGCTTTTGCATTTCAATGGGGTTTATCTGCGCAACATGGCTCCTGCTGAGTTGGCGCAACGGGTTGCACCCTATCTCAG
CAAGGCAGGCTTGATCAGCGCCGAACCAACTGCTGCCGAATTGGCCAAAATTACCGAATATTTGCCATTGGTGCAAGATC
GCCTAAAATTGCTCAGTGAAGCGCCAGAATTGCTCGATTTCTTCTTTGTAGACCCACAAGGCTACGACCCAGCCTTGCTT
GTGCCAAAGAAGGGCGATCCGGCGCAAACGGTTGAGATACTGGGCCAAGTCAAAGCTAGTTTTGAGGCTGTCGAAACTTG
GGATGCCCCCAGTTTAGACAAGCTCTTGCATGATTTTGTCAATCAACTTGGGCTGAAAATTCCCCAAGTGTTTATGCCTA
TTCGCGTTGCCATCAGTGGGCGCACGACCTCGCCAGGCCTATTCGAAACCTTGGCGGTTTTAGGTAAAGCTGTGACGCTC
GCCCGAATCAGCACTGCTGCTGCTGCGCTTTCCTCTGCCTCTGTATAG

Upstream 100 bases:

>100_bases
CTACTTGTCAACGTGGCGTAGCTGTTTAACGCGAGAAGGGTTCCGCGCCAATATCACCGCCAGCAATGGCTATGGTATAA
TCGCGACGCAAGGAGGTATT

Downstream 100 bases:

>100_bases
TTCGATTAGTCAGGAGGATGTTCAATGACGCTTGACGAAGCCAAAACCGAATTGACCAACTTGGTCTTGGGAGTTTCGCC
CGATGCCGTGTTACGCTATA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSDRPTPARTRFAPSPTGYLHIGSLRTVLFSWLWARHTGGQFLLRIEDTDRKRFVEGAEEQLTSSLQAIGLMWDEGPIVG
GPHAPYKQSERLEIYQAHAQALIDKGVAYRSYATADEIAAINAEREARGEPKLLVFRNLPGIDDAAREAAGADYNVRLSL
KTTGQTVVQDLVRGQIVFDNAALKMPDPVLLKTDGFPTYALAAMVDDHLMGITHVLRADEWIPTWPIHHQIYEAFGWEQP
VWVHVPQVLGSDGKKLSKRHGDTSVTEYIDLGFVPEAIINYLALIGWSYDDKTEFMTLEELIERFDLNRIRPSGGVFDRD
KLLHFNGVYLRNMAPAELAQRVAPYLSKAGLISAEPTAAELAKITEYLPLVQDRLKLLSEAPELLDFFFVDPQGYDPALL
VPKKGDPAQTVEILGQVKASFEAVETWDAPSLDKLLHDFVNQLGLKIPQVFMPIRVAISGRTTSPGLFETLAVLGKAVTL
ARISTAAAALSSASV

Sequences:

>Translated_495_residues
MSDRPTPARTRFAPSPTGYLHIGSLRTVLFSWLWARHTGGQFLLRIEDTDRKRFVEGAEEQLTSSLQAIGLMWDEGPIVG
GPHAPYKQSERLEIYQAHAQALIDKGVAYRSYATADEIAAINAEREARGEPKLLVFRNLPGIDDAAREAAGADYNVRLSL
KTTGQTVVQDLVRGQIVFDNAALKMPDPVLLKTDGFPTYALAAMVDDHLMGITHVLRADEWIPTWPIHHQIYEAFGWEQP
VWVHVPQVLGSDGKKLSKRHGDTSVTEYIDLGFVPEAIINYLALIGWSYDDKTEFMTLEELIERFDLNRIRPSGGVFDRD
KLLHFNGVYLRNMAPAELAQRVAPYLSKAGLISAEPTAAELAKITEYLPLVQDRLKLLSEAPELLDFFFVDPQGYDPALL
VPKKGDPAQTVEILGQVKASFEAVETWDAPSLDKLLHDFVNQLGLKIPQVFMPIRVAISGRTTSPGLFETLAVLGKAVTL
ARISTAAAALSSASV
>Mature_494_residues
SDRPTPARTRFAPSPTGYLHIGSLRTVLFSWLWARHTGGQFLLRIEDTDRKRFVEGAEEQLTSSLQAIGLMWDEGPIVGG
PHAPYKQSERLEIYQAHAQALIDKGVAYRSYATADEIAAINAEREARGEPKLLVFRNLPGIDDAAREAAGADYNVRLSLK
TTGQTVVQDLVRGQIVFDNAALKMPDPVLLKTDGFPTYALAAMVDDHLMGITHVLRADEWIPTWPIHHQIYEAFGWEQPV
WVHVPQVLGSDGKKLSKRHGDTSVTEYIDLGFVPEAIINYLALIGWSYDDKTEFMTLEELIERFDLNRIRPSGGVFDRDK
LLHFNGVYLRNMAPAELAQRVAPYLSKAGLISAEPTAAELAKITEYLPLVQDRLKLLSEAPELLDFFFVDPQGYDPALLV
PKKGDPAQTVEILGQVKASFEAVETWDAPSLDKLLHDFVNQLGLKIPQVFMPIRVAISGRTTSPGLFETLAVLGKAVTLA
RISTAAAALSSASV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=499, Percent_Identity=31.6633266533066, Blast_Score=242, Evalue=4e-64,
Organism=Escherichia coli, GI1788743, Length=486, Percent_Identity=35.8024691358025, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=34.4, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786895, Length=374, Percent_Identity=25.1336898395722, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=36.1746361746362, Blast_Score=268, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6324540, Length=495, Percent_Identity=33.5353535353535, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24665321, Length=510, Percent_Identity=34.9019607843137, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_HERA2 (A9AXJ1)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001543533.1
- ProteinModelPortal:   A9AXJ1
- SMR:   A9AXJ1
- GeneID:   5732641
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_0757
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- BioCyc:   HAUR316274:HAUR_0757-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54778; Mature: 54647

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRPTPARTRFAPSPTGYLHIGSLRTVLFSWLWARHTGGQFLLRIEDTDRKRFVEGAEE
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCHHH
QLTSSLQAIGLMWDEGPIVGGPHAPYKQSERLEIYQAHAQALIDKGVAYRSYATADEIAA
HHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHC
INAEREARGEPKLLVFRNLPGIDDAAREAAGADYNVRLSLKTTGQTVVQDLVRGQIVFDN
CCCHHHCCCCCCEEEEECCCCCCHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCEEEECC
AALKMPDPVLLKTDGFPTYALAAMVDDHLMGITHVLRADEWIPTWPIHHQIYEAFGWEQP
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
VWVHVPQVLGSDGKKLSKRHGDTSVTEYIDLGFVPEAIINYLALIGWSYDDKTEFMTLEE
EEEEEHHHHCCCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LIERFDLNRIRPSGGVFDRDKLLHFNGVYLRNMAPAELAQRVAPYLSKAGLISAEPTAAE
HHHHHCCCCCCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHH
LAKITEYLPLVQDRLKLLSEAPELLDFFFVDPQGYDPALLVPKKGDPAQTVEILGQVKAS
HHHHHHHHHHHHHHHHHHHHCHHHHHEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHH
FEAVETWDAPSLDKLLHDFVNQLGLKIPQVFMPIRVAISGRTTSPGLFETLAVLGKAVTL
HHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHH
ARISTAAAALSSASV
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDRPTPARTRFAPSPTGYLHIGSLRTVLFSWLWARHTGGQFLLRIEDTDRKRFVEGAEE
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCHHH
QLTSSLQAIGLMWDEGPIVGGPHAPYKQSERLEIYQAHAQALIDKGVAYRSYATADEIAA
HHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHC
INAEREARGEPKLLVFRNLPGIDDAAREAAGADYNVRLSLKTTGQTVVQDLVRGQIVFDN
CCCHHHCCCCCCEEEEECCCCCCHHHHHHCCCCEEEEEEEECCHHHHHHHHHCCEEEECC
AALKMPDPVLLKTDGFPTYALAAMVDDHLMGITHVLRADEWIPTWPIHHQIYEAFGWEQP
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
VWVHVPQVLGSDGKKLSKRHGDTSVTEYIDLGFVPEAIINYLALIGWSYDDKTEFMTLEE
EEEEEHHHHCCCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
LIERFDLNRIRPSGGVFDRDKLLHFNGVYLRNMAPAELAQRVAPYLSKAGLISAEPTAAE
HHHHHCCCCCCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHH
LAKITEYLPLVQDRLKLLSEAPELLDFFFVDPQGYDPALLVPKKGDPAQTVEILGQVKAS
HHHHHHHHHHHHHHHHHHHHCHHHHHEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHH
FEAVETWDAPSLDKLLHDFVNQLGLKIPQVFMPIRVAISGRTTSPGLFETLAVLGKAVTL
HHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHH
ARISTAAAALSSASV
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA