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The map label for this gene is yqiG [H]

Identifier: 159897025

GI number: 159897025

Start: 575668

End: 576774

Strand: Reverse

Name: yqiG [H]

Synonym: Haur_0493

Alternate gene names: 159897025

Gene position: 576774-575668 (Counterclockwise)

Preceding gene: 159897026

Following gene: 159897023

Centisome position: 9.09

GC content: 54.02

Gene sequence:

>1107_bases
ATGTCATTGTTCGAGCCATATACGCTGCCTGTTAGCGGTGTTACCCTGCCCAATCGTCTCGTTCTCGCCCCCATGACCAC
CTATTCGGGCACGCCTGAAGGCCAAATTAGCCCAGGTGAAGTGGCTTTATTGCAACGCCGTGCCGCTGGCGGCTTTGGAA
CCGTTATCACCGCTGCTTGTTACGTTGATAGGGCTGGCCAAGCCTTTATCAATCAATGGGGCTGCGATAATGACGACAAG
CTCGATTCATTACGTCAAGCAGCTAGCGCCATCAAGGCCGAAGGCGCAATCGCCATTCTGCAAATTCACGATGGCGGGCG
CATGGCCGACCCCAAGGTGTTGATCGAGCCAGCGCCACGCTCAGCCTCAGCCGTTGCCGCCGAACGCCAAGGCTCCCTCG
AACCTCGGGCAATGAGCGCTGCTGAAGTCGAAAAAAGCATCGCCGATTTTGTGGCAGCCGCCAAACGGGCAGCAGTCGCA
GGCTTCGATGGCGTTGAAATTCACGGCGCAAATACCTATTTGATTCAACAATTTTTCTCGCCGCATTCCAACCGCCGCGA
CGACGAATGGGGTGGCGACTTGGAACGACGCTTGCGCTACCCAATTACTTTAGCCAAAGCGGTGCGCGAAGCCGTTGGCG
ATCAGTTTATTGTGGGCTATCGCTTCTCGCCAGAGGAGATCGAAAATCCAGGCATTACGATTGAAGATACCTTAGTTTTG
GTTGATCGTTTAGCCGATTGCCGCTTGGATTACTTGCACATTTCTTTATGGAATTATGCGGCTGGCTCGATGCGCAACCC
CGAATCAACCCAACGACTGAGCCCTTTAGTCATTGAAAAATTGGCTGGGCGGGTTCCCTTAATGGCAGTCGGCAGCATCG
AAACGCCTGAACAAGCCGAATTACCCTTGCACGATGGAGCCGAACTCGTGGCACTTGGACGGGTCGCTTTGTGGGAGCCA
GACTGGCCAAATAAGGTTAAAGCAGGCCGCAGTGATTTACGCCAAGCCTTGCCCGCCAAAGATGGCGATGTGATTTGTAG
CTTACCAAAGCCATTGTATGAGCTGTTAATCCAACGCACTGGCTGGGTCAAAGTCGCCGCTGAGTAA

Upstream 100 bases:

>100_bases
AATTCTTCTCTAGTCTGCCCGTTTTCAAAATCGACTTGTGGCACAATATATTGGGATACGCATATCTGCGTAATTCAATA
TATTGTGTTACGAGGAATTT

Downstream 100 bases:

>100_bases
GGAGCAAGCTTTAACGCAGAGGCGCAGAGTGGATCATTTCTTTCCCTCACCCCCTCGCCCCCTCCTTACAAGGGTAGGTT
GTTAACAATGCTTGAGTGGG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSLFEPYTLPVSGVTLPNRLVLAPMTTYSGTPEGQISPGEVALLQRRAAGGFGTVITAACYVDRAGQAFINQWGCDNDDK
LDSLRQAASAIKAEGAIAILQIHDGGRMADPKVLIEPAPRSASAVAAERQGSLEPRAMSAAEVEKSIADFVAAAKRAAVA
GFDGVEIHGANTYLIQQFFSPHSNRRDDEWGGDLERRLRYPITLAKAVREAVGDQFIVGYRFSPEEIENPGITIEDTLVL
VDRLADCRLDYLHISLWNYAAGSMRNPESTQRLSPLVIEKLAGRVPLMAVGSIETPEQAELPLHDGAELVALGRVALWEP
DWPNKVKAGRSDLRQALPAKDGDVICSLPKPLYELLIQRTGWVKVAAE

Sequences:

>Translated_368_residues
MSLFEPYTLPVSGVTLPNRLVLAPMTTYSGTPEGQISPGEVALLQRRAAGGFGTVITAACYVDRAGQAFINQWGCDNDDK
LDSLRQAASAIKAEGAIAILQIHDGGRMADPKVLIEPAPRSASAVAAERQGSLEPRAMSAAEVEKSIADFVAAAKRAAVA
GFDGVEIHGANTYLIQQFFSPHSNRRDDEWGGDLERRLRYPITLAKAVREAVGDQFIVGYRFSPEEIENPGITIEDTLVL
VDRLADCRLDYLHISLWNYAAGSMRNPESTQRLSPLVIEKLAGRVPLMAVGSIETPEQAELPLHDGAELVALGRVALWEP
DWPNKVKAGRSDLRQALPAKDGDVICSLPKPLYELLIQRTGWVKVAAE
>Mature_367_residues
SLFEPYTLPVSGVTLPNRLVLAPMTTYSGTPEGQISPGEVALLQRRAAGGFGTVITAACYVDRAGQAFINQWGCDNDDKL
DSLRQAASAIKAEGAIAILQIHDGGRMADPKVLIEPAPRSASAVAAERQGSLEPRAMSAAEVEKSIADFVAAAKRAAVAG
FDGVEIHGANTYLIQQFFSPHSNRRDDEWGGDLERRLRYPITLAKAVREAVGDQFIVGYRFSPEEIENPGITIEDTLVLV
DRLADCRLDYLHISLWNYAAGSMRNPESTQRLSPLVIEKLAGRVPLMAVGSIETPEQAELPLHDGAELVALGRVALWEPD
WPNKVKAGRSDLRQALPAKDGDVICSLPKPLYELLIQRTGWVKVAAE

Specific function: Catalyzes The NADP-Dependent Reduction Of 2,4-Dienoyl- CoA To Yield Trans-2- Enoyl-CoA. [C]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=337, Percent_Identity=28.7833827893175, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787939, Length=343, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=359, Percent_Identity=30.9192200557103, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17540738, Length=353, Percent_Identity=28.6118980169972, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17564188, Length=346, Percent_Identity=29.1907514450867, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=347, Percent_Identity=28.5302593659942, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI72001454, Length=339, Percent_Identity=28.023598820059, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17565138, Length=346, Percent_Identity=25.7225433526012, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17566914, Length=327, Percent_Identity=27.5229357798165, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17566916, Length=347, Percent_Identity=26.5129682997118, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=352, Percent_Identity=27.8409090909091, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321973, Length=357, Percent_Identity=26.890756302521, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 39905; Mature: 39774

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFEPYTLPVSGVTLPNRLVLAPMTTYSGTPEGQISPGEVALLQRRAAGGFGTVITAAC
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
YVDRAGQAFINQWGCDNDDKLDSLRQAASAIKAEGAIAILQIHDGGRMADPKVLIEPAPR
HHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCC
SASAVAAERQGSLEPRAMSAAEVEKSIADFVAAAKRAAVAGFDGVEIHGANTYLIQQFFS
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHC
PHSNRRDDEWGGDLERRLRYPITLAKAVREAVGDQFIVGYRFSPEEIENPGITIEDTLVL
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCHHHHHHH
VDRLADCRLDYLHISLWNYAAGSMRNPESTQRLSPLVIEKLAGRVPLMAVGSIETPEQAE
HHHHHHCCEEEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
LPLHDGAELVALGRVALWEPDWPNKVKAGRSDLRQALPAKDGDVICSLPKPLYELLIQRT
CCCCCCCCEEEECCEEEECCCCCCHHHCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHCC
GWVKVAAE
CCEEEECC
>Mature Secondary Structure 
SLFEPYTLPVSGVTLPNRLVLAPMTTYSGTPEGQISPGEVALLQRRAAGGFGTVITAAC
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
YVDRAGQAFINQWGCDNDDKLDSLRQAASAIKAEGAIAILQIHDGGRMADPKVLIEPAPR
HHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCCC
SASAVAAERQGSLEPRAMSAAEVEKSIADFVAAAKRAAVAGFDGVEIHGANTYLIQQFFS
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHC
PHSNRRDDEWGGDLERRLRYPITLAKAVREAVGDQFIVGYRFSPEEIENPGITIEDTLVL
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCHHHHHHH
VDRLADCRLDYLHISLWNYAAGSMRNPESTQRLSPLVIEKLAGRVPLMAVGSIETPEQAE
HHHHHHCCEEEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
LPLHDGAELVALGRVALWEPDWPNKVKAGRSDLRQALPAKDGDVICSLPKPLYELLIQRT
CCCCCCCCEEEECCEEEECCCCCCHHHCCHHHHHHHCCCCCCCEEECCCHHHHHHHHHCC
GWVKVAAE
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]