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The map label for this gene is yisK [H]

Identifier: 159897022

GI number: 159897022

Start: 571828

End: 572697

Strand: Reverse

Name: yisK [H]

Synonym: Haur_0490

Alternate gene names: 159897022

Gene position: 572697-571828 (Counterclockwise)

Preceding gene: 159897023

Following gene: 159897021

Centisome position: 9.02

GC content: 50.11

Gene sequence:

>870_bases
ATGCGATTTGTAACCCTCAAAACTGCGGTTGGGCCACGTCCAGGCATTGTGCTTGGCGATCGGGTGATGTTGCTCGATGG
CTATCAATCGCTCCAAGCCTTAATCGAAGCAGGCGATGCTGGCTTGGACACAATCAAGGCGGCCTTGCCCGACTATCAAA
GCCGTGCAGGCATCCAATTAAATATGGATCAATTGTTGGCTCCCTTGCCCCGCCCATTGAAAAATGTGTTTTGTGTGGGC
CTTAATTATGCAGCCCACGCTCGTGAATCGTTGCAAGCCAAAGGGCTAGAAGTCAAAATGCCCGAGCATCCGGTATTTTT
CACCAAACCACCAACCGCCATCAACAGCCCAACTGGCGAAATTGTGATTGATCCAGCAGTTTCCGAACGGATTGATTGGG
AAGTTGAATTGGGTGTGGTGATCGGCAAAGCTGGCAAAAACATCAGCCACGAGCAAGCCATGGAGCATGTTTGGGGCTAT
ACGGTGATTAACGATGTTTCGGCGCGAGATTTACAAATGCGCCATCAGCAATTTTTCAAAGGCAAAGCGCTCGATGGCTC
ATGCCCAATGGGGCCGTGGATCATCACCAGCGATGAGTTGACCGACCCGCATAATTTGGTGGTTCGGCTACGCGTCAACG
GCGAGATCAAACAGGAGTCTAATACCAACGATCTTATTTTTAATATTCCTACATTAATTCATGTGCTTTCCCAAGGCATG
ACCCTTGAGCCAGGCGATATTATTGCAACTGGCACACCGGCTGGCGTAGGTTTTGCGCGTACTCCCCAAGAATTTTTACG
CCCAGGCGATTTGCTCGAAACCGAAGTTGAGGGCATTGGTATTCTTCGTAACCCTGTCGTGGCAGGCTAA

Upstream 100 bases:

>100_bases
GCGTAGCATCACCAGAAACGAATATCGTTATAATGCAACTATAGTTTTTTTGCCAAACGATAATGTGAAACCGTTATCAT
GGCGATGTTGCGGAGAAACC

Downstream 100 bases:

>100_bases
TGTTTATAAAAACAGCATTCTGACAAAACTACATTATTTCCAGAATGCTGTTTTTTTGTGGCCTAAATCAGATATTTAAT
CATTCTATCGCCATAATAAA

Product: 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRFVTLKTAVGPRPGIVLGDRVMLLDGYQSLQALIEAGDAGLDTIKAALPDYQSRAGIQLNMDQLLAPLPRPLKNVFCVG
LNYAAHARESLQAKGLEVKMPEHPVFFTKPPTAINSPTGEIVIDPAVSERIDWEVELGVVIGKAGKNISHEQAMEHVWGY
TVINDVSARDLQMRHQQFFKGKALDGSCPMGPWIITSDELTDPHNLVVRLRVNGEIKQESNTNDLIFNIPTLIHVLSQGM
TLEPGDIIATGTPAGVGFARTPQEFLRPGDLLETEVEGIGILRNPVVAG

Sequences:

>Translated_289_residues
MRFVTLKTAVGPRPGIVLGDRVMLLDGYQSLQALIEAGDAGLDTIKAALPDYQSRAGIQLNMDQLLAPLPRPLKNVFCVG
LNYAAHARESLQAKGLEVKMPEHPVFFTKPPTAINSPTGEIVIDPAVSERIDWEVELGVVIGKAGKNISHEQAMEHVWGY
TVINDVSARDLQMRHQQFFKGKALDGSCPMGPWIITSDELTDPHNLVVRLRVNGEIKQESNTNDLIFNIPTLIHVLSQGM
TLEPGDIIATGTPAGVGFARTPQEFLRPGDLLETEVEGIGILRNPVVAG
>Mature_289_residues
MRFVTLKTAVGPRPGIVLGDRVMLLDGYQSLQALIEAGDAGLDTIKAALPDYQSRAGIQLNMDQLLAPLPRPLKNVFCVG
LNYAAHARESLQAKGLEVKMPEHPVFFTKPPTAINSPTGEIVIDPAVSERIDWEVELGVVIGKAGKNISHEQAMEHVWGY
TVINDVSARDLQMRHQQFFKGKALDGSCPMGPWIITSDELTDPHNLVVRLRVNGEIKQESNTNDLIFNIPTLIHVLSQGM
TLEPGDIIATGTPAGVGFARTPQEFLRPGDLLETEVEGIGILRNPVVAG

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=298, Percent_Identity=41.6107382550336, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI40786394, Length=298, Percent_Identity=40.9395973154362, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI215422413, Length=188, Percent_Identity=40.9574468085106, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI66348062, Length=214, Percent_Identity=38.785046728972, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI13654274, Length=188, Percent_Identity=40.9574468085106, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1787428, Length=195, Percent_Identity=38.4615384615385, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=40.1069518716578, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324161, Length=237, Percent_Identity=27.0042194092827, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28572127, Length=298, Percent_Identity=41.2751677852349, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24663695, Length=224, Percent_Identity=37.5, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28571789, Length=205, Percent_Identity=36.5853658536585, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 31437; Mature: 31437

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFVTLKTAVGPRPGIVLGDRVMLLDGYQSLQALIEAGDAGLDTIKAALPDYQSRAGIQL
CEEEEEEECCCCCCCEEECCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCHHCCCCEE
NMDQLLAPLPRPLKNVFCVGLNYAAHARESLQAKGLEVKMPEHPVFFTKPPTAINSPTGE
CHHHHHHCCCHHHHHHEEEECCHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCCCCC
IVIDPAVSERIDWEVELGVVIGKAGKNISHEQAMEHVWGYTVINDVSARDLQMRHQQFFK
EEECCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCHHEECCCCHHHHHHHHHHHHC
GKALDGSCPMGPWIITSDELTDPHNLVVRLRVNGEIKQESNTNDLIFNIPTLIHVLSQGM
CCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCEEECCCCCCCEEEEHHHHHHHHHCCC
TLEPGDIIATGTPAGVGFARTPQEFLRPGDLLETEVEGIGILRNPVVAG
EECCCCEEEECCCCCCCCCCCCHHHCCCCCHHHHHHCCCEEECCCCCCC
>Mature Secondary Structure
MRFVTLKTAVGPRPGIVLGDRVMLLDGYQSLQALIEAGDAGLDTIKAALPDYQSRAGIQL
CEEEEEEECCCCCCCEEECCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCHHCCCCEE
NMDQLLAPLPRPLKNVFCVGLNYAAHARESLQAKGLEVKMPEHPVFFTKPPTAINSPTGE
CHHHHHHCCCHHHHHHEEEECCHHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCCCCCC
IVIDPAVSERIDWEVELGVVIGKAGKNISHEQAMEHVWGYTVINDVSARDLQMRHQQFFK
EEECCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCHHEECCCCHHHHHHHHHHHHC
GKALDGSCPMGPWIITSDELTDPHNLVVRLRVNGEIKQESNTNDLIFNIPTLIHVLSQGM
CCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCEEECCCCCCCEEEEHHHHHHHHHCCC
TLEPGDIIATGTPAGVGFARTPQEFLRPGDLLETEVEGIGILRNPVVAG
EECCCCEEEECCCCCCCCCCCCHHHCCCCCHHHHHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]