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The map label for this gene is nikB [H]

Identifier: 159896901

GI number: 159896901

Start: 441790

End: 442740

Strand: Reverse

Name: nikB [H]

Synonym: Haur_0369

Alternate gene names: 159896901

Gene position: 442740-441790 (Counterclockwise)

Preceding gene: 159896902

Following gene: 159896900

Centisome position: 6.98

GC content: 54.47

Gene sequence:

>951_bases
ATGAGCACGCTATTGCGGCAATCTGCGGGGCGCTTGCTCAGTGGGCTAATGGTCTTATGGATGGTCAGCATGCTGGCATT
TCTGATCATCCATATGGCTCCAGGTGATCCAGCGCGAATGTTGTTGAGCGCCAGCGGCCTTGATGCACCACCCGCCGAAG
CTGTTGCAGCCAAACGACGTGAGCTAGGGCTTGATCGTCCGCTGCTCAGCCAATATGTCAGCTGGCTTGGCGCGGTGGTG
CAGGGCGATTTTGGGGTCAGCTATCGTTCGGGCAAACCAGTTTGGTCGATGTATCGCGAACGGTTGCCCGCCACCTTACT
GATGGCGGGCTTGGTGCTAACAGTTTCGCTGAGCATTGCCATTCCTTTGGGATTGATCAGCGCGATCAAGCGCCATAGTT
TGTTGGATCATGCGGCGCAATTTTTGGCCGTGATTGGCGCGGCAATTCCTGGCTTTTGGATTGCGCTGGTGTTGATTTTG
GTGTTTGCGGCCAAATTGCGTTGGTTACCAGCCCTCGGCAGCGCCACATTTAAAGGCATGCTGCTGCCAGCCATCGTTTT
AGCCTTGCCGAATATAGCGCTAACCTCACGTTTGGTACGCGCAACCACCCTCGACGTGCTAAATCAACAGTATATCGTGA
CGGCTCGAGCCAAAGGGCGCTCCGAATATGGTTTGCTCTTTGGGCATGCGCTTCCCAACATTGCGGCCAGTTTGATCACC
CCAATCAGCCTAGAATTGGCCTTTTTGCTAACTGGCACGGTAATTATTGAAACAGTATTTGCCTATCCTGGGGTTGGGCG
GATGGCGGTTGAGGCAGCCTTGGTCGGCGATATGCCAGTTTTGGGCTTGTCAGTCTTGATCGCAGGGGGAATTTATGTTA
TCGCTAACTGGTGCGCTGACCTCGGCATGGCAGCGCTTGACCCACGGCTCCGCCAAAACGCCAATAATTGA

Upstream 100 bases:

>100_bases
GTCCACCACTGATCAGCGCCTATCGCAAGGGCAGCATCAGTGGCTATGTGCCCAATCCCAACGATGTCTATTTGATTACC
AAGGAATTGAAACTCAATCA

Downstream 100 bases:

>100_bases
GCAGCCCGAGCAACGTGCCAAGCCACAACTAAGCCGCAGCGGCAAATTTGCCGCTGGCGGATTGTTGTTTATGCTGATAG
CGGTGATCGTTGGCCCATGG

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSTLLRQSAGRLLSGLMVLWMVSMLAFLIIHMAPGDPARMLLSASGLDAPPAEAVAAKRRELGLDRPLLSQYVSWLGAVV
QGDFGVSYRSGKPVWSMYRERLPATLLMAGLVLTVSLSIAIPLGLISAIKRHSLLDHAAQFLAVIGAAIPGFWIALVLIL
VFAAKLRWLPALGSATFKGMLLPAIVLALPNIALTSRLVRATTLDVLNQQYIVTARAKGRSEYGLLFGHALPNIAASLIT
PISLELAFLLTGTVIIETVFAYPGVGRMAVEAALVGDMPVLGLSVLIAGGIYVIANWCADLGMAALDPRLRQNANN

Sequences:

>Translated_316_residues
MSTLLRQSAGRLLSGLMVLWMVSMLAFLIIHMAPGDPARMLLSASGLDAPPAEAVAAKRRELGLDRPLLSQYVSWLGAVV
QGDFGVSYRSGKPVWSMYRERLPATLLMAGLVLTVSLSIAIPLGLISAIKRHSLLDHAAQFLAVIGAAIPGFWIALVLIL
VFAAKLRWLPALGSATFKGMLLPAIVLALPNIALTSRLVRATTLDVLNQQYIVTARAKGRSEYGLLFGHALPNIAASLIT
PISLELAFLLTGTVIIETVFAYPGVGRMAVEAALVGDMPVLGLSVLIAGGIYVIANWCADLGMAALDPRLRQNANN
>Mature_315_residues
STLLRQSAGRLLSGLMVLWMVSMLAFLIIHMAPGDPARMLLSASGLDAPPAEAVAAKRRELGLDRPLLSQYVSWLGAVVQ
GDFGVSYRSGKPVWSMYRERLPATLLMAGLVLTVSLSIAIPLGLISAIKRHSLLDHAAQFLAVIGAAIPGFWIALVLILV
FAAKLRWLPALGSATFKGMLLPAIVLALPNIALTSRLVRATTLDVLNQQYIVTARAKGRSEYGLLFGHALPNIAASLITP
ISLELAFLLTGTVIIETVFAYPGVGRMAVEAALVGDMPVLGLSVLIAGGIYVIANWCADLGMAALDPRLRQNANN

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=291, Percent_Identity=39.5189003436426, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1787053, Length=310, Percent_Identity=34.5161290322581, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789965, Length=323, Percent_Identity=31.5789473684211, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787761, Length=316, Percent_Identity=34.8101265822785, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787497, Length=305, Percent_Identity=29.5081967213115, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=29.0441176470588, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788504, Length=356, Percent_Identity=27.247191011236, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33611; Mature: 33480

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLLRQSAGRLLSGLMVLWMVSMLAFLIIHMAPGDPARMLLSASGLDAPPAEAVAAKRR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
ELGLDRPLLSQYVSWLGAVVQGDFGVSYRSGKPVWSMYRERLPATLLMAGLVLTVSLSIA
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
IPLGLISAIKRHSLLDHAAQFLAVIGAAIPGFWIALVLILVFAAKLRWLPALGSATFKGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LLPAIVLALPNIALTSRLVRATTLDVLNQQYIVTARAKGRSEYGLLFGHALPNIAASLIT
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
PISLELAFLLTGTVIIETVFAYPGVGRMAVEAALVGDMPVLGLSVLIAGGIYVIANWCAD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGMAALDPRLRQNANN
CCHHHCCHHHHCCCCC
>Mature Secondary Structure 
STLLRQSAGRLLSGLMVLWMVSMLAFLIIHMAPGDPARMLLSASGLDAPPAEAVAAKRR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
ELGLDRPLLSQYVSWLGAVVQGDFGVSYRSGKPVWSMYRERLPATLLMAGLVLTVSLSIA
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
IPLGLISAIKRHSLLDHAAQFLAVIGAAIPGFWIALVLILVFAAKLRWLPALGSATFKGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LLPAIVLALPNIALTSRLVRATTLDVLNQQYIVTARAKGRSEYGLLFGHALPNIAASLIT
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
PISLELAFLLTGTVIIETVFAYPGVGRMAVEAALVGDMPVLGLSVLIAGGIYVIANWCAD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LGMAALDPRLRQNANN
CCHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]