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The map label for this gene is cysW [H]

Identifier: 159896791

GI number: 159896791

Start: 301935

End: 302774

Strand: Reverse

Name: cysW [H]

Synonym: Haur_0258

Alternate gene names: 159896791

Gene position: 302774-301935 (Counterclockwise)

Preceding gene: 159896792

Following gene: 159896790

Centisome position: 4.77

GC content: 53.33

Gene sequence:

>840_bases
ATGACGACTCAAACAATTGAACGCACGGCCCCACGTTTACCGTGGGGCCGTTGGAGTATCCGTGGCGCTGCCTTAACCTA
TTTGGCAGTGTTTTTGGTATTGCCGTTAATTGTAGTCGTCGTCGATGGATTGGGCGCTGGCGTTGAATCGATCGTCAGCG
TGGTTAGCTCGCCAATTGCCCGCCACGCAATTTGGTTGACGATTTGGACTGGCCTCGTGATGTCGTTGATCAATGCAGTA
ATGGGCTTATTGACAGCATTCGTTTTGGTGCGTTATGAGTTTCCGGGCAAAACCTTATTGAATGCAGTCGTTGATTTGCC
GTTTGCTATCCCCACCTTGGTCACGGGCGTGATGCTGGTGATTTTGTATGGGCCACAAGCGGCAATCGGCGGCTGGCTCA
ATCAACAGTTGGGTTGGCAAGTGATTTTTGCGCCCTCTGGCATCATTTTGGCCTTGCTGTTTACCTCATTTCCATTCGTG
GTGCGCTCGGTTCAGCCAGTCTTGATCAACCTTGATCGTTCGAGCGAAGAGGCAGCGGCAACGCTTGGCGCACCTGCTTG
GCAAATTTTTTGGCGCGTGACTCTGCCAGCCTTGTTGCCAGCCTTGGTTTCGGGCACATTGCTGAGTTTTGCGCGAGCCA
TTGGCGAATTTGGTGCGATTGTCGTGGTTGCTGGCAATATTCCGCTGCGCTCACAAACTGCCGCTGTCTATGTTTATGGC
GAGATTGAATCGGGCAGCCAACGCGATGCTAGCGCGATGTCGTTGGTGATGTTGGCAATTGCGCTTGGCACGATGAGCTT
GGTCGATTGGCTCAGTCGGCGGCGGGAGGTGCAAGCATGA

Upstream 100 bases:

>100_bases
ATTTGTTTACAATCAACGAATTTGGTGGTTGGAGCAAAGCCACGCCTGAATACTTTGGCGATGACGGTGTCTATGCCAAA
GTGCTAGCGCAGGTACAACA

Downstream 100 bases:

>100_bases
ATTGGCGCAAATGGCTGTTGATCAGCATTGTCGTAGGCTACATGGGCATTTTGATCATCGCCCCGCTGGGTGCAATTGTG
TTTGGTGCGTTTCGCGAAGG

Product: sulfate ABC transporter inner membrane subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTTQTIERTAPRLPWGRWSIRGAALTYLAVFLVLPLIVVVVDGLGAGVESIVSVVSSPIARHAIWLTIWTGLVMSLINAV
MGLLTAFVLVRYEFPGKTLLNAVVDLPFAIPTLVTGVMLVILYGPQAAIGGWLNQQLGWQVIFAPSGIILALLFTSFPFV
VRSVQPVLINLDRSSEEAAATLGAPAWQIFWRVTLPALLPALVSGTLLSFARAIGEFGAIVVVAGNIPLRSQTAAVYVYG
EIESGSQRDASAMSLVMLAIALGTMSLVDWLSRRREVQA

Sequences:

>Translated_279_residues
MTTQTIERTAPRLPWGRWSIRGAALTYLAVFLVLPLIVVVVDGLGAGVESIVSVVSSPIARHAIWLTIWTGLVMSLINAV
MGLLTAFVLVRYEFPGKTLLNAVVDLPFAIPTLVTGVMLVILYGPQAAIGGWLNQQLGWQVIFAPSGIILALLFTSFPFV
VRSVQPVLINLDRSSEEAAATLGAPAWQIFWRVTLPALLPALVSGTLLSFARAIGEFGAIVVVAGNIPLRSQTAAVYVYG
EIESGSQRDASAMSLVMLAIALGTMSLVDWLSRRREVQA
>Mature_278_residues
TTQTIERTAPRLPWGRWSIRGAALTYLAVFLVLPLIVVVVDGLGAGVESIVSVVSSPIARHAIWLTIWTGLVMSLINAVM
GLLTAFVLVRYEFPGKTLLNAVVDLPFAIPTLVTGVMLVILYGPQAAIGGWLNQQLGWQVIFAPSGIILALLFTSFPFVV
RSVQPVLINLDRSSEEAAATLGAPAWQIFWRVTLPALLPALVSGTLLSFARAIGEFGAIVVVAGNIPLRSQTAAVYVYGE
IESGSQRDASAMSLVMLAIALGTMSLVDWLSRRREVQA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=282, Percent_Identity=35.1063829787234, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788764, Length=222, Percent_Identity=43.2432432432432, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1786980, Length=213, Percent_Identity=35.2112676056338, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786254, Length=218, Percent_Identity=25.6880733944954, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787369, Length=237, Percent_Identity=25.7383966244726, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787368, Length=208, Percent_Identity=27.8846153846154, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29923; Mature: 29792

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTIERTAPRLPWGRWSIRGAALTYLAVFLVLPLIVVVVDGLGAGVESIVSVVSSPIA
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RHAIWLTIWTGLVMSLINAVMGLLTAFVLVRYEFPGKTLLNAVVDLPFAIPTLVTGVMLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
ILYGPQAAIGGWLNQQLGWQVIFAPSGIILALLFTSFPFVVRSVQPVLINLDRSSEEAAA
HHCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
TLGAPAWQIFWRVTLPALLPALVSGTLLSFARAIGEFGAIVVVAGNIPLRSQTAAVYVYG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEE
EIESGSQRDASAMSLVMLAIALGTMSLVDWLSRRREVQA
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTQTIERTAPRLPWGRWSIRGAALTYLAVFLVLPLIVVVVDGLGAGVESIVSVVSSPIA
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RHAIWLTIWTGLVMSLINAVMGLLTAFVLVRYEFPGKTLLNAVVDLPFAIPTLVTGVMLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
ILYGPQAAIGGWLNQQLGWQVIFAPSGIILALLFTSFPFVVRSVQPVLINLDRSSEEAAA
HHCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
TLGAPAWQIFWRVTLPALLPALVSGTLLSFARAIGEFGAIVVVAGNIPLRSQTAAVYVYG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEEE
EIESGSQRDASAMSLVMLAIALGTMSLVDWLSRRREVQA
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]