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The map label for this gene is cysW [H]

Identifier: 159896790

GI number: 159896790

Start: 301129

End: 301938

Strand: Reverse

Name: cysW [H]

Synonym: Haur_0257

Alternate gene names: 159896790

Gene position: 301938-301129 (Counterclockwise)

Preceding gene: 159896791

Following gene: 159896788

Centisome position: 4.76

GC content: 50.74

Gene sequence:

>810_bases
ATGAATTGGCGCAAATGGCTGTTGATCAGCATTGTCGTAGGCTACATGGGCATTTTGATCATCGCCCCGCTGGGTGCAAT
TGTGTTTGGTGCGTTTCGCGAAGGCGCTCCGGCAGTGCTAGAGGCCTTGCAACATCCCCATGTGTTACGCTCATTTGGCC
TAACCTTGATCATCTGTTTGGTAGTAGTGTCAATTCATGGCATCTTTGGCACGCTAGTGGCTTGGGTACTGGTGCGTCAT
CAATTTGTCGGAAAAGCCTTGGTTAATCGATTGCTCGATTTGCCATTCGCTATGTCGCCAGTGATCGTTGGTTTCACCTT
AATTGTGCTGTTTGGGCGTAATGGTGTGCTCAAGCCACTAACCGAGGCGCTTGATTGGCAAATTGCCTTTGCTTTGCCTG
GAATGATTTTGGCGACCTTGTTTGTAACCTTGCCGTTTATGGTGCGCGAGTTGATGCCTGTGCTTGAGGCATTTGGGCGC
AAACAGGAAGAGGCTGCTGCAACCTTGGGTGCCAGTGGCTGGCAAACCTTCCGCTTAGTCACATTTCCAGCCTTGCGTTG
GGCTTTTTTATATGGTTTAGGCCTGACCTGTGCCCGCGCCTTGGGCGAATTTGGGGCGGTGCTGGTGATCAGCGGTGGAA
TTCAAGGACGCACCGAAACTGCCACGGTCTACATCTTTCGGGCGCTCGATGATCGCCAATATGCCGGAGCTTATAGTGCA
GCCTTGCTACTAGGGGCAATTTCATTAGTCTTAGTATTAGTCATCGATGTGTTTCATCGGCGTTCACAAAAGAGTATAGA
GCAACGCTAG

Upstream 100 bases:

>100_bases
GCCAACGCGATGCTAGCGCGATGTCGTTGGTGATGTTGGCAATTGCGCTTGGCACGATGAGCTTGGTCGATTGGCTCAGT
CGGCGGCGGGAGGTGCAAGC

Downstream 100 bases:

>100_bases
GGATTAGAGGTCAGGGATGAGGGGCCAGGTGTAATGGTTTATAACGTTAACAACGCCCAGCATGCCCTCACCCCCAGCCC
CTCTCACACGGCGGCGGGCG

Product: sulfate ABC transporter inner membrane subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNWRKWLLISIVVGYMGILIIAPLGAIVFGAFREGAPAVLEALQHPHVLRSFGLTLIICLVVVSIHGIFGTLVAWVLVRH
QFVGKALVNRLLDLPFAMSPVIVGFTLIVLFGRNGVLKPLTEALDWQIAFALPGMILATLFVTLPFMVRELMPVLEAFGR
KQEEAAATLGASGWQTFRLVTFPALRWAFLYGLGLTCARALGEFGAVLVISGGIQGRTETATVYIFRALDDRQYAGAYSA
ALLLGAISLVLVLVIDVFHRRSQKSIEQR

Sequences:

>Translated_269_residues
MNWRKWLLISIVVGYMGILIIAPLGAIVFGAFREGAPAVLEALQHPHVLRSFGLTLIICLVVVSIHGIFGTLVAWVLVRH
QFVGKALVNRLLDLPFAMSPVIVGFTLIVLFGRNGVLKPLTEALDWQIAFALPGMILATLFVTLPFMVRELMPVLEAFGR
KQEEAAATLGASGWQTFRLVTFPALRWAFLYGLGLTCARALGEFGAVLVISGGIQGRTETATVYIFRALDDRQYAGAYSA
ALLLGAISLVLVLVIDVFHRRSQKSIEQR
>Mature_269_residues
MNWRKWLLISIVVGYMGILIIAPLGAIVFGAFREGAPAVLEALQHPHVLRSFGLTLIICLVVVSIHGIFGTLVAWVLVRH
QFVGKALVNRLLDLPFAMSPVIVGFTLIVLFGRNGVLKPLTEALDWQIAFALPGMILATLFVTLPFMVRELMPVLEAFGR
KQEEAAATLGASGWQTFRLVTFPALRWAFLYGLGLTCARALGEFGAVLVISGGIQGRTETATVYIFRALDDRQYAGAYSA
ALLLGAISLVLVLVIDVFHRRSQKSIEQR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=269, Percent_Identity=42.0074349442379, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI1788764, Length=221, Percent_Identity=38.4615384615385, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1786980, Length=189, Percent_Identity=37.5661375661376, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786254, Length=224, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29445; Mature: 29445

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50928 ABC_TM1 ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWRKWLLISIVVGYMGILIIAPLGAIVFGAFREGAPAVLEALQHPHVLRSFGLTLIICL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHH
VVVSIHGIFGTLVAWVLVRHQFVGKALVNRLLDLPFAMSPVIVGFTLIVLFGRNGVLKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
TEALDWQIAFALPGMILATLFVTLPFMVRELMPVLEAFGRKQEEAAATLGASGWQTFRLV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHH
TFPALRWAFLYGLGLTCARALGEFGAVLVISGGIQGRTETATVYIFRALDDRQYAGAYSA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHEEEEEECCCCHHHHHHHH
ALLLGAISLVLVLVIDVFHRRSQKSIEQR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNWRKWLLISIVVGYMGILIIAPLGAIVFGAFREGAPAVLEALQHPHVLRSFGLTLIICL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHH
VVVSIHGIFGTLVAWVLVRHQFVGKALVNRLLDLPFAMSPVIVGFTLIVLFGRNGVLKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
TEALDWQIAFALPGMILATLFVTLPFMVRELMPVLEAFGRKQEEAAATLGASGWQTFRLV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHH
TFPALRWAFLYGLGLTCARALGEFGAVLVISGGIQGRTETATVYIFRALDDRQYAGAYSA
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHEEEEEECCCCHHHHHHHH
ALLLGAISLVLVLVIDVFHRRSQKSIEQR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]