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The map label for this gene is wcaL [C]

Identifier: 159896767

GI number: 159896767

Start: 269886

End: 271043

Strand: Direct

Name: wcaL [C]

Synonym: Haur_0234

Alternate gene names: 159896767

Gene position: 269886-271043 (Clockwise)

Preceding gene: 159896766

Following gene: 159896768

Centisome position: 4.25

GC content: 51.47

Gene sequence:

>1158_bases
ATGTTCTATAATGTGCTCAGCAATTATTTGAGCGCGAGTATCACCCTAGCAATGCATATTCTTCATGTCTATAAAGATTA
TTTTCCAGTGCTTGGTGGCATGGAAAACCATATTCGGGTAGTGGCTGAGGGCTTGGCTGAACGCGGCCATCAAGTTACGG
TGGCAGTCAGCAATACCTACCCAAAAACCGAAATCGAGCGCCGTAATGGTGTAGCGATTATTAAAGCAGCCCAATGGTTG
CGCAAGGCATCAACCCCGATTAGCCCGATGAGTTTGCCATTAAGTTGGCGTGTACCCGCCGATATTATCCATTTGCATCA
TCCCTTCCCACCTGGCGATTTGCTGTATTGGCTGCGTGGTGGCAAGGCTAAATTGGTGATTACTTATCAAAGCGATATTG
TGCGCCAACGCCGTTTGTTGCAACTCTATCGACCATTGCTTACCCGTACTTTGAACGCCGCCGATCGAATTATCGCGGCC
AGCCCGCAGTATATCCAAACCTCGCCATGGTTGGCTCCTCATGCCGCCAAATGCCGCGTAATTCCCTTGAGCGTCGATAC
CGAGCGCTTCAATCAACTTGATCATGCGGCGATTCAGGCGTTGCGTGAGCAGGTTGCAGCACCCATGGTGTTGTTTGTTG
GGCGCTTCCGCCATTACAAAGGCCTGCACTTTTTGCTCGAAGCCTTGCCAAAAATTCCCAAGGCCAAATTGGTGTTGGTC
GGCATTGGCCCTGAGGAAGCTCGTTTGCGCGAGTTGGCGCAACGCTTGGGTGTTGGCGAACGTATTATATGGGCTGGCGA
AGTCCCGGATCAAGCCTTACCAAATTACTATGCCGCTGCCGATGTATTTGTGCTACCATCTCATTTACGAGCAGAAGCAT
TTGGCATCGTGCAACTCGAAGCATTAGCCGCTGGAATTCCAATTGTCAGCACTGAGTTGGGCACTGGCACAAGTTTTGTC
AACGCCCACGGCCAAACTGGGTTTGTTGTGCCACCAGCCGATCCGGCGGCACTGGCGCGGGCAATCACTGTGCTGTTGGA
AAATCCAGGCTTGCGGGCGCAATTTGGAGCTAACGGTCGTCAACGCGCGAGCAGCACGTTCAGTCCACAGCGCATGCTCG
ATCAGATTGAAGAACTTTATCGTGAGATTGTGAGTTAG

Upstream 100 bases:

>100_bases
TAATCTCTGTTCTTTGCGCCGCTGCGTTAAACCTGCCTACGATCCCTCGACCCTTCCCTCCCATTTCATCCTTCATCCCT
CATCCCTCATCCTTTTCTCA

Downstream 100 bases:

>100_bases
GTATGTTGCTTCAAGCAGGAACCGAATTGTTGGGGCGTTATCACATCGTGCGGGCAATCGGCCAAGGCGGTATGAGCGCT
GTCTATGCAGCGTTTGATCG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MFYNVLSNYLSASITLAMHILHVYKDYFPVLGGMENHIRVVAEGLAERGHQVTVAVSNTYPKTEIERRNGVAIIKAAQWL
RKASTPISPMSLPLSWRVPADIIHLHHPFPPGDLLYWLRGGKAKLVITYQSDIVRQRRLLQLYRPLLTRTLNAADRIIAA
SPQYIQTSPWLAPHAAKCRVIPLSVDTERFNQLDHAAIQALREQVAAPMVLFVGRFRHYKGLHFLLEALPKIPKAKLVLV
GIGPEEARLRELAQRLGVGERIIWAGEVPDQALPNYYAAADVFVLPSHLRAEAFGIVQLEALAAGIPIVSTELGTGTSFV
NAHGQTGFVVPPADPAALARAITVLLENPGLRAQFGANGRQRASSTFSPQRMLDQIEELYREIVS

Sequences:

>Translated_385_residues
MFYNVLSNYLSASITLAMHILHVYKDYFPVLGGMENHIRVVAEGLAERGHQVTVAVSNTYPKTEIERRNGVAIIKAAQWL
RKASTPISPMSLPLSWRVPADIIHLHHPFPPGDLLYWLRGGKAKLVITYQSDIVRQRRLLQLYRPLLTRTLNAADRIIAA
SPQYIQTSPWLAPHAAKCRVIPLSVDTERFNQLDHAAIQALREQVAAPMVLFVGRFRHYKGLHFLLEALPKIPKAKLVLV
GIGPEEARLRELAQRLGVGERIIWAGEVPDQALPNYYAAADVFVLPSHLRAEAFGIVQLEALAAGIPIVSTELGTGTSFV
NAHGQTGFVVPPADPAALARAITVLLENPGLRAQFGANGRQRASSTFSPQRMLDQIEELYREIVS
>Mature_385_residues
MFYNVLSNYLSASITLAMHILHVYKDYFPVLGGMENHIRVVAEGLAERGHQVTVAVSNTYPKTEIERRNGVAIIKAAQWL
RKASTPISPMSLPLSWRVPADIIHLHHPFPPGDLLYWLRGGKAKLVITYQSDIVRQRRLLQLYRPLLTRTLNAADRIIAA
SPQYIQTSPWLAPHAAKCRVIPLSVDTERFNQLDHAAIQALREQVAAPMVLFVGRFRHYKGLHFLLEALPKIPKAKLVLV
GIGPEEARLRELAQRLGVGERIIWAGEVPDQALPNYYAAADVFVLPSHLRAEAFGIVQLEALAAGIPIVSTELGTGTSFV
NAHGQTGFVVPPADPAALARAITVLLENPGLRAQFGANGRQRASSTFSPQRMLDQIEELYREIVS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=222, Percent_Identity=26.5765765765766, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42661; Mature: 42661

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYNVLSNYLSASITLAMHILHVYKDYFPVLGGMENHIRVVAEGLAERGHQVTVAVSNTY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCC
PKTEIERRNGVAIIKAAQWLRKASTPISPMSLPLSWRVPADIIHLHHPFPPGDLLYWLRG
CCCHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCEECCCCHHHEEECCCCCCCCEEEEEEC
GKAKLVITYQSDIVRQRRLLQLYRPLLTRTLNAADRIIAASPQYIQTSPWLAPHAAKCRV
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCCCEEE
IPLSVDTERFNQLDHAAIQALREQVAAPMVLFVGRFRHYKGLHFLLEALPKIPKAKLVLV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GIGPEEARLRELAQRLGVGERIIWAGEVPDQALPNYYAAADVFVLPSHLRAEAFGIVQLE
ECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHHHHEEECCHHHHHHHHHEEHH
ALAAGIPIVSTELGTGTSFVNAHGQTGFVVPPADPAALARAITVLLENPGLRAQFGANGR
HHHCCCCEEEECCCCCCCEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCH
QRASSTFSPQRMLDQIEELYREIVS
HHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFYNVLSNYLSASITLAMHILHVYKDYFPVLGGMENHIRVVAEGLAERGHQVTVAVSNTY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCC
PKTEIERRNGVAIIKAAQWLRKASTPISPMSLPLSWRVPADIIHLHHPFPPGDLLYWLRG
CCCHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCEECCCCHHHEEECCCCCCCCEEEEEEC
GKAKLVITYQSDIVRQRRLLQLYRPLLTRTLNAADRIIAASPQYIQTSPWLAPHAAKCRV
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCCCEEE
IPLSVDTERFNQLDHAAIQALREQVAAPMVLFVGRFRHYKGLHFLLEALPKIPKAKLVLV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
GIGPEEARLRELAQRLGVGERIIWAGEVPDQALPNYYAAADVFVLPSHLRAEAFGIVQLE
ECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHHHHEEECCHHHHHHHHHEEHH
ALAAGIPIVSTELGTGTSFVNAHGQTGFVVPPADPAALARAITVLLENPGLRAQFGANGR
HHHCCCCEEEECCCCCCCEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCH
QRASSTFSPQRMLDQIEELYREIVS
HHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]