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The map label for this gene is pspA [C]

Identifier: 159896765

GI number: 159896765

Start: 268064

End: 268723

Strand: Reverse

Name: pspA [C]

Synonym: Haur_0232

Alternate gene names: 159896765

Gene position: 268723-268064 (Counterclockwise)

Preceding gene: 159896773

Following gene: 159896759

Centisome position: 4.23

GC content: 49.7

Gene sequence:

>660_bases
ATGTGGAAACGAATCAAAGACCTTATCCGCGCTTGGTTGCACGCCTTGTTTGATCGCGCTGAAAACCCCGTAACCATGGC
CGATGAGTATATTCGCCAGCTTGAGGCGCAATATGGCGAGGCCAAACGTGCAACGGCTATGGCCATGGGTCAGGCCACAC
GTATGGAGCAAAAGCGCGATGAAAACCGTAAGCAAGCCGAGGATTGGGAACGCAAAGCTGTCAATGCGCTTGAACGTGGC
GACGAAGAATTGGCTCGTTCAGCTTTGGAACGCAAGCGCCACTTCAGCGCTGCCGCCAGCGAATATGCAACCCAATCGGA
AACTCAAAATGCCCAAGTTAAAGATTTTCGGAATTCGATCAGCCGTTTGGAACTGCAAATTAATGAAGCTCGCGCCAAAC
GCGATTTGATCAAAGCTAAAACTAGCCGTGCCGATTCGCAAGAAGCCGCCACTCGTGCGCTGCAAAATATGCAAGGCAGT
GGCGAATTGGGCGATCGCTTGGGAGCAATGGAAGAACGAATTGATGATCGGTTGTACAAAGCTGAAGCCATGGCCAAATT
AGAAGGCGATTCGTTGGAAAATAAATTTCGCAATTTGGAGCAAGAAACCTCGCTCGAATCAGAATTGGCAGCGCTCAAAG
CTAAAGTCAATCAAAGCTAA

Upstream 100 bases:

>100_bases
GGTTGATCCTTGCGTTTGCTCTTGCAGATCAGGTATGCTTAAGCAACATTCCAAAGGCTGGCCCGTACTACCCAACAGTA
GTCAGTAGGAGCAAAACGCT

Downstream 100 bases:

>100_bases
ATCTGTTAATTTGATTGAGCACAAAACCCTCTGTTTGTTGATCGCAGAGGGTTTTTAATTATTGTTACGTGCCAGCCAGT
ACATCGGCCACGCTCAGCCA

Product: phage shock protein PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MWKRIKDLIRAWLHALFDRAENPVTMADEYIRQLEAQYGEAKRATAMAMGQATRMEQKRDENRKQAEDWERKAVNALERG
DEELARSALERKRHFSAAASEYATQSETQNAQVKDFRNSISRLELQINEARAKRDLIKAKTSRADSQEAATRALQNMQGS
GELGDRLGAMEERIDDRLYKAEAMAKLEGDSLENKFRNLEQETSLESELAALKAKVNQS

Sequences:

>Translated_219_residues
MWKRIKDLIRAWLHALFDRAENPVTMADEYIRQLEAQYGEAKRATAMAMGQATRMEQKRDENRKQAEDWERKAVNALERG
DEELARSALERKRHFSAAASEYATQSETQNAQVKDFRNSISRLELQINEARAKRDLIKAKTSRADSQEAATRALQNMQGS
GELGDRLGAMEERIDDRLYKAEAMAKLEGDSLENKFRNLEQETSLESELAALKAKVNQS
>Mature_219_residues
MWKRIKDLIRAWLHALFDRAENPVTMADEYIRQLEAQYGEAKRATAMAMGQATRMEQKRDENRKQAEDWERKAVNALERG
DEELARSALERKRHFSAAASEYATQSETQNAQVKDFRNSISRLELQINEARAKRDLIKAKTSRADSQEAATRALQNMQGS
GELGDRLGAMEERIDDRLYKAEAMAKLEGDSLENKFRNLEQETSLESELAALKAKVNQS

Specific function: The Phage Shock Protein (Psp) Operon (Pspabce) May Play A Significant Role In The Competition For Survival Under Nutrient- Or Energy-Limited Conditions. Pspa Has A Role As A Negative Regulator, Is Required For Efficient Translocation And Has An Unknown Fu

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=221, Percent_Identity=25.3393665158371, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25065; Mature: 25065

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKRIKDLIRAWLHALFDRAENPVTMADEYIRQLEAQYGEAKRATAMAMGQATRMEQKRD
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENRKQAEDWERKAVNALERGDEELARSALERKRHFSAAASEYATQSETQNAQVKDFRNSI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SRLELQINEARAKRDLIKAKTSRADSQEAATRALQNMQGSGELGDRLGAMEERIDDRLYK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AEAMAKLEGDSLENKFRNLEQETSLESELAALKAKVNQS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWKRIKDLIRAWLHALFDRAENPVTMADEYIRQLEAQYGEAKRATAMAMGQATRMEQKRD
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENRKQAEDWERKAVNALERGDEELARSALERKRHFSAAASEYATQSETQNAQVKDFRNSI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SRLELQINEARAKRDLIKAKTSRADSQEAATRALQNMQGSGELGDRLGAMEERIDDRLYK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AEAMAKLEGDSLENKFRNLEQETSLESELAALKAKVNQS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]