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The map label for this gene is rpoN [H]

Identifier: 159896658

GI number: 159896658

Start: 157444

End: 158829

Strand: Direct

Name: rpoN [H]

Synonym: Haur_0125

Alternate gene names: 159896658

Gene position: 157444-158829 (Clockwise)

Preceding gene: 159896656

Following gene: 159896659

Centisome position: 2.48

GC content: 49.86

Gene sequence:

>1386_bases
ATGGAATTTCGAATGGGTATGTCGCCGGAGGCGCTAGCACAACAAGCCATGTCAACCAAAGCCTCGCCCGCTTTGATTGC
ACTTAACAATATGCTGGTGCTTTCAACCCACGAATTGCAACAATTGATCCAACAAGAGCTTGAGGAGAATCCCGCTTTAG
AAAATGTTGACCTAGAGCCAGATGAAACCTGCTCGCGTTGTGGCCGCCCCTTGATTGAGATGCTCTGTTTATACTGCGTT
CATGAAGATCAGCGCTTGGCCGAGGCTGAACGTGGCGATTATACCGCTGCTGGCGAAGACGATGAATTTGATCCATTGAT
GATGGTGGCGACTCCCGCTCAACTTAGTGAGGTGTTGCTACGCGATTTACGCGCTTCGTTGCCTGAGCAAGATTATTTTA
TCGCTGAATATTTGGTTGGCTGTTTGGATGACCAAGGCTTTCTCGATGCCAATACCGGCGATATTGCGGCAACCCTGACG
ATCGATGAAGAACGGGTGATTGCAGTCTTGCTCAAATTGCAAGATATTGCTCCAGTTGGAGTTGGGGCGCGTGATGTGCA
AGAATGTTTGTTGTTGCAGTTGCGCCGTTTGGATGAAGAAGATGTGCGCCATCCCTATGTTGAACGAGTGATCACCGAGT
TTTGGAATGATTTGGCTGAGCACCGCTATGGGGCAATTGCCCAGGCGCTCAATGCCGAATACGATGCTGTGGTTGAAGTG
CGCGAGTTTATTCGTGAACATTTGCGCCCCTATCCGTTGGCGCTCAGTGGCGAAGGCTCAGCCCGCACCAACTATATTAT
GCCCGATGTGATTATTCGTGAGAACGAGGGCAAACTCGAGGCCGAAGTGCTGATGTCGGATCGCAGCTTTTTGCGGATTA
ATCCGCTATATCAATCGTTGGTCAAACGTGGGCGCGAAGCTGAGGCTGAAATGTCTTCCGAAGATCGCGATCATCTTAGT
CAGTATGTGGCACGTGCTCAAATGTTCCTGACCAATATTCGCCAACGCCGCGAAACTATCCGTCGGATTAGTGAATTTTT
GATTGAAACTCAAGAAGATTTCTTGCGCCAAGGCGTGCGGTTCTTGCGGCCAATGACCCGCGCTGAAGTGGCTGATGGCA
TTGGTGTCCACGAATCGACCGTGAGCCGTGCGACCGCCAATAAATATGTCCAATTGCCCAATCGCTCGGTAATTCCGTTT
AGCCATTTCTTCACGGCCTCGTTGAATGTCAAGGATGTGCTGCTAGAATTGGTGCAAAATGAAGTGCGACCACTCACCGA
CCAAGAATTAGTCGAATTGTTGGCTGAACGTGGCTTTGAGGTGGCGCGGCGCACGATTGCCAAATATCGTAATCAATTGA
ATATTTTGCCCTCAACCTTGCGCTAA

Upstream 100 bases:

>100_bases
GCAGTCGTTCTACGATGGTTTACCGTATAATAACAATAGCATTTGCATGTTTGCCAGCATCATGTGTTGGCCAAGCACCA
ACCTATGGAGGGGTTGCGCC

Downstream 100 bases:

>100_bases
GCAGCGGTTTGAGGAAAGGGTGTGGGATGCAGCGTTGGCGACATTGGCTCGAATGGTTGGTGCTCGGCGGATTAAGTGTA
TTAATCGCCCGTTTGATGTT

Product: RNA polymerase sigma-54 subunit RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MEFRMGMSPEALAQQAMSTKASPALIALNNMLVLSTHELQQLIQQELEENPALENVDLEPDETCSRCGRPLIEMLCLYCV
HEDQRLAEAERGDYTAAGEDDEFDPLMMVATPAQLSEVLLRDLRASLPEQDYFIAEYLVGCLDDQGFLDANTGDIAATLT
IDEERVIAVLLKLQDIAPVGVGARDVQECLLLQLRRLDEEDVRHPYVERVITEFWNDLAEHRYGAIAQALNAEYDAVVEV
REFIREHLRPYPLALSGEGSARTNYIMPDVIIRENEGKLEAEVLMSDRSFLRINPLYQSLVKRGREAEAEMSSEDRDHLS
QYVARAQMFLTNIRQRRETIRRISEFLIETQEDFLRQGVRFLRPMTRAEVADGIGVHESTVSRATANKYVQLPNRSVIPF
SHFFTASLNVKDVLLELVQNEVRPLTDQELVELLAERGFEVARRTIAKYRNQLNILPSTLR

Sequences:

>Translated_461_residues
MEFRMGMSPEALAQQAMSTKASPALIALNNMLVLSTHELQQLIQQELEENPALENVDLEPDETCSRCGRPLIEMLCLYCV
HEDQRLAEAERGDYTAAGEDDEFDPLMMVATPAQLSEVLLRDLRASLPEQDYFIAEYLVGCLDDQGFLDANTGDIAATLT
IDEERVIAVLLKLQDIAPVGVGARDVQECLLLQLRRLDEEDVRHPYVERVITEFWNDLAEHRYGAIAQALNAEYDAVVEV
REFIREHLRPYPLALSGEGSARTNYIMPDVIIRENEGKLEAEVLMSDRSFLRINPLYQSLVKRGREAEAEMSSEDRDHLS
QYVARAQMFLTNIRQRRETIRRISEFLIETQEDFLRQGVRFLRPMTRAEVADGIGVHESTVSRATANKYVQLPNRSVIPF
SHFFTASLNVKDVLLELVQNEVRPLTDQELVELLAERGFEVARRTIAKYRNQLNILPSTLR
>Mature_461_residues
MEFRMGMSPEALAQQAMSTKASPALIALNNMLVLSTHELQQLIQQELEENPALENVDLEPDETCSRCGRPLIEMLCLYCV
HEDQRLAEAERGDYTAAGEDDEFDPLMMVATPAQLSEVLLRDLRASLPEQDYFIAEYLVGCLDDQGFLDANTGDIAATLT
IDEERVIAVLLKLQDIAPVGVGARDVQECLLLQLRRLDEEDVRHPYVERVITEFWNDLAEHRYGAIAQALNAEYDAVVEV
REFIREHLRPYPLALSGEGSARTNYIMPDVIIRENEGKLEAEVLMSDRSFLRINPLYQSLVKRGREAEAEMSSEDRDHLS
QYVARAQMFLTNIRQRRETIRRISEFLIETQEDFLRQGVRFLRPMTRAEVADGIGVHESTVSRATANKYVQLPNRSVIPF
SHFFTASLNVKDVLLELVQNEVRPLTDQELVELLAERGFEVARRTIAKYRNQLNILPSTLR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 a

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=474, Percent_Identity=29.957805907173, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 52581; Mature: 52581

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRMGMSPEALAQQAMSTKASPALIALNNMLVLSTHELQQLIQQELEENPALENVDLEP
CCCCCCCCHHHHHHHHHHCCCCCCEEEECCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCC
DETCSRCGRPLIEMLCLYCVHEDQRLAEAERGDYTAAGEDDEFDPLMMVATPAQLSEVLL
CHHHHHHCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHH
RDLRASLPEQDYFIAEYLVGCLDDQGFLDANTGDIAATLTIDEERVIAVLLKLQDIAPVG
HHHHHHCCCCHHHHHHHHHHHCCCCCCEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCC
VGARDVQECLLLQLRRLDEEDVRHPYVERVITEFWNDLAEHRYGAIAQALNAEYDAVVEV
CCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
REFIREHLRPYPLALSGEGSARTNYIMPDVIIRENEGKLEAEVLMSDRSFLRINPLYQSL
HHHHHHHCCCCCEEECCCCCCCCCEECCCEEEECCCCCHHHHHHHCCCCCEEECHHHHHH
VKRGREAEAEMSSEDRDHLSQYVARAQMFLTNIRQRRETIRRISEFLIETQEDFLRQGVR
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRPMTRAEVADGIGVHESTVSRATANKYVQLPNRSVIPFSHFFTASLNVKDVLLELVQN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCCCCCHHHHEECCCHHHHHHHHHHH
EVRPLTDQELVELLAERGFEVARRTIAKYRNQLNILPSTLR
HCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEFRMGMSPEALAQQAMSTKASPALIALNNMLVLSTHELQQLIQQELEENPALENVDLEP
CCCCCCCCHHHHHHHHHHCCCCCCEEEECCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCC
DETCSRCGRPLIEMLCLYCVHEDQRLAEAERGDYTAAGEDDEFDPLMMVATPAQLSEVLL
CHHHHHHCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHH
RDLRASLPEQDYFIAEYLVGCLDDQGFLDANTGDIAATLTIDEERVIAVLLKLQDIAPVG
HHHHHHCCCCHHHHHHHHHHHCCCCCCEECCCCCEEEEEEECHHHHHHHHHHHHHCCCCC
VGARDVQECLLLQLRRLDEEDVRHPYVERVITEFWNDLAEHRYGAIAQALNAEYDAVVEV
CCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
REFIREHLRPYPLALSGEGSARTNYIMPDVIIRENEGKLEAEVLMSDRSFLRINPLYQSL
HHHHHHHCCCCCEEECCCCCCCCCEECCCEEEECCCCCHHHHHHHCCCCCEEECHHHHHH
VKRGREAEAEMSSEDRDHLSQYVARAQMFLTNIRQRRETIRRISEFLIETQEDFLRQGVR
HHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRPMTRAEVADGIGVHESTVSRATANKYVQLPNRSVIPFSHFFTASLNVKDVLLELVQN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCCCCCHHHHEECCCHHHHHHHHHHH
EVRPLTDQELVELLAERGFEVARRTIAKYRNQLNILPSTLR
HCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2602128; 12534463 [H]