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The map label for this gene is lysX [H]

Identifier: 159041863

GI number: 159041863

Start: 1371848

End: 1372663

Strand: Reverse

Name: lysX [H]

Synonym: Cmaq_1298

Alternate gene names: 159041863

Gene position: 1372663-1371848 (Counterclockwise)

Preceding gene: 159041864

Following gene: 159041859

Centisome position: 66.07

GC content: 44.36

Gene sequence:

>816_bases
GTGATTTCTATTGAAGTCCACATCCTCTACGACATAATTAGACTGGAGGAGAAGCTTCTTATTAATGAGCTGGGTAAGCG
AGGATTAAGCGTTAGGCCTATTAGGGCTGAATCCATTAATATTAGTAATACTAGCATTAATGCATTCGGGTTAATTAGGG
TGGCTGGTAGGAGTAAGGTTTTGCCATTATCATTCACCTACCAGGAACTAGGGCTTGGGTCAATTAACCCATTCAACAGC
CTATACATAACGCACGATAAGTTCCTCACCCTAGTTAGGTTGATTAAAGCTGGTGTACCGGTACCGAGGTCAATGCTCCT
ACTGGATCATGAGGAGGGGGCTTTAAGTGAACTTGGGGCACCGTTAATAATAAAGCCAACTGATGGTTCATGGGGTAGGT
TAGTTGCCTTAGCCAACACTGCCAGGGAAGCTGGTTTAATAATTAGGAGTTACAGTGATGATGTATTACTGGGTCAGGAG
TATGTTAGGAAGCCTAAGCGGGACATTAGGGTTACTGTTGTTGGTGATAGGGCAGTGGCCGCAATCTATAGGTATAGTGA
TGATTGGAGAACCAACACTGCAAGGGGTGGTTCAGCAGCCCCTGTTAAGATTGACCCTGAGCTTGAGGATATTTCAGTTA
AGGCCAGTAAGGCTACTGGTTCAGTGTACTCAGGTGTGGATGTTGTTGAGTCTGATAGGGGTTACCTTGTTCTTGAGGTT
AATGGTGTGCCTGAATTCAAGAATGTTCAAAGGGTTACTGGGATTAATGTTGCCGGTGAAATAGCTGAGGAGGTGGCTAG
GTTAATCATCTCTTAA

Upstream 100 bases:

>100_bases
CAGTTGCCCAACATGCGGTACAAAGTACATTGTGAAGATTAGTAAGGATAAGCTTGAACTCGAGGAGTTTAAGGGGGATG
TTGAGGATTACGGTGAATAG

Downstream 100 bases:

>100_bases
TCCACTCACTGACAGCCTCAGCCAGCTTAAAACTAACCCTAACACCACTGACCTTGCTTATTGCCTTAAATTCAGGGATG
CCGTTAATCTCACTTATATA

Product: lysine biosynthesis enzyme LysX

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MISIEVHILYDIIRLEEKLLINELGKRGLSVRPIRAESINISNTSINAFGLIRVAGRSKVLPLSFTYQELGLGSINPFNS
LYITHDKFLTLVRLIKAGVPVPRSMLLLDHEEGALSELGAPLIIKPTDGSWGRLVALANTAREAGLIIRSYSDDVLLGQE
YVRKPKRDIRVTVVGDRAVAAIYRYSDDWRTNTARGGSAAPVKIDPELEDISVKASKATGSVYSGVDVVESDRGYLVLEV
NGVPEFKNVQRVTGINVAGEIAEEVARLIIS

Sequences:

>Translated_271_residues
MISIEVHILYDIIRLEEKLLINELGKRGLSVRPIRAESINISNTSINAFGLIRVAGRSKVLPLSFTYQELGLGSINPFNS
LYITHDKFLTLVRLIKAGVPVPRSMLLLDHEEGALSELGAPLIIKPTDGSWGRLVALANTAREAGLIIRSYSDDVLLGQE
YVRKPKRDIRVTVVGDRAVAAIYRYSDDWRTNTARGGSAAPVKIDPELEDISVKASKATGSVYSGVDVVESDRGYLVLEV
NGVPEFKNVQRVTGINVAGEIAEEVARLIIS
>Mature_271_residues
MISIEVHILYDIIRLEEKLLINELGKRGLSVRPIRAESINISNTSINAFGLIRVAGRSKVLPLSFTYQELGLGSINPFNS
LYITHDKFLTLVRLIKAGVPVPRSMLLLDHEEGALSELGAPLIIKPTDGSWGRLVALANTAREAGLIIRSYSDDVLLGQE
YVRKPKRDIRVTVVGDRAVAAIYRYSDDWRTNTARGGSAAPVKIDPELEDISVKASKATGSVYSGVDVVESDRGYLVLEV
NGVPEFKNVQRVTGINVAGEIAEEVARLIIS

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=212, Percent_Identity=28.3018867924528, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787076, Length=211, Percent_Identity=32.7014218009479, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 29649; Mature: 29649

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIEVHILYDIIRLEEKLLINELGKRGLSVRPIRAESINISNTSINAFGLIRVAGRSKV
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEECCCCCCCEEEEEEECCCCEE
LPLSFTYQELGLGSINPFNSLYITHDKFLTLVRLIKAGVPVPRSMLLLDHEEGALSELGA
EEEEEEHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCC
PLIIKPTDGSWGRLVALANTAREAGLIIRSYSDDVLLGQEYVRKPKRDIRVTVVGDRAVA
CEEEEECCCCCCEEEEEECCCHHCCEEEEECCCCEEECHHHHHCCCCCEEEEEECCCEEE
AIYRYSDDWRTNTARGGSAAPVKIDPELEDISVKASKATGSVYSGVDVVESDRGYLVLEV
EEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHCCCEEEECCCCEEEEEE
NGVPEFKNVQRVTGINVAGEIAEEVARLIIS
CCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISIEVHILYDIIRLEEKLLINELGKRGLSVRPIRAESINISNTSINAFGLIRVAGRSKV
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEECEEECCCCCCCEEEEEEECCCCEE
LPLSFTYQELGLGSINPFNSLYITHDKFLTLVRLIKAGVPVPRSMLLLDHEEGALSELGA
EEEEEEHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCC
PLIIKPTDGSWGRLVALANTAREAGLIIRSYSDDVLLGQEYVRKPKRDIRVTVVGDRAVA
CEEEEECCCCCCEEEEEECCCHHCCEEEEECCCCEEECHHHHHCCCCCEEEEEECCCEEE
AIYRYSDDWRTNTARGGSAAPVKIDPELEDISVKASKATGSVYSGVDVVESDRGYLVLEV
EEEEECCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHCCCEEEECCCCEEEEEE
NGVPEFKNVQRVTGINVAGEIAEEVARLIIS
CCCCCHHCCHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]