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The map label for this gene is aglA [H]

Identifier: 159041780

GI number: 159041780

Start: 1281040

End: 1282425

Strand: Reverse

Name: aglA [H]

Synonym: Cmaq_1215

Alternate gene names: 159041780

Gene position: 1282425-1281040 (Counterclockwise)

Preceding gene: 159041781

Following gene: 159041777

Centisome position: 61.73

GC content: 43.8

Gene sequence:

>1386_bases
ATGAGCGAACAAATTAAGAACATAAAGATTTGTATAATAGGTGGAGGCAGTCATACGTTTATAGCAAGTATACTTAGGGA
TATTGCATTAACAAAGAGTATTCATGGAATCACACTAACCCTAATGGATATTGATGAACATAGATTAGCTAGAAGCTACA
TGCTGGCCAGGAAGTATTTCGATGAACTTAAGGTACCCATTAATCTAGAAAGAACCACTGATACTAAGGCTTGTATTGAA
GGCGCCTCCTTCGTAATTAACCTAGCCTTCGCAATAGGTTACGATCACTGGGGCATTCAGGTTGAGGCTGCTGAGAGGCA
TGGGTACTATAGGGGTATTGATGCAACTGAGTGGAATATGGTGTGCTGCTACCCATCATTAACCGGGTTTAAGCAGTATA
ATGTGGCGTTGAAAATAGCAGGCATAATGGATGAGATTAATAGGGATGCTTGGTTAATTCAAGTCTCCAACCCAGTTCTC
GAAACAACAACTTTAGTACATAGGCAGTATCCTAAGCTTAAAATTATTGGTTACTGCCATGGAGCGCCCGGCGGTGTTAG
ATTATTGGTTGAGAAGGCGTTGAAACTTGATATGAGGAGGATTGAGTGGCAGGCGGTTGGCTTAAATCACGTGGTGTTTC
TAACTAGGTTTAAGTATAATGGTGAAGACGCCTACCACTTGATTGATGAGTGGATTGAGAAGAAGGCTGAGGAATTCTGG
GCTAGTTACGTGCCTGGCCCATGGGAAGAGACCTTGAGTAGGGCTGCTGTGGACATGTATAGACTCTACGGCCTATACCC
ACTTGGTGACACGGCTAGGAGTGGGACGTGGAAGTACCATAGGGATCTTAAAACCAAGATATATTGGTATGGACCCATTG
GTGGTGTTGATTCTGAGGTAGGGTGGGGGATTAGGATGCTTAGGAATCAGGAGGCTGAGGCTAAGTTGGAGAATGCTGCA
TTCAACCCAAGCATTAAGGCCACTGAGGCTTATCCACCGGTTAAGAGTGGTGAGCAGATTATTGATTTCATAGATAGTGT
TGTTAATAACGTTGAGAGGAGAATGATACTAAACATACCCAATAATGGCGTATTACCTAGACTACCAAGCGACGCCATAG
TTGAGGCGCCGGTGTACGTTAAGGGTGAGGTAATTAGGCCTGAGGCCATTGAGAATGTACCAAATAAAATGTACTCATAC
GTATGGTACCCTAGAATAGCCGTCACTGAGAGGGCACTTGAAGCCTACTTAGCTGGTAGCAAGGAGTTACTTATTGAAGC
ATTGATGTTCGACCCAAGGACCAAGAGCACTGAACAGGCGAGGGAGGTTATTGATGAAATACTGAACCTACCGTTTAACG
AGGATATGAAGAAGCACTATAAGTGA

Upstream 100 bases:

>100_bases
ACTCATTTTAATGCATATCAATATTATTATCCTTAAACATGTTCAGTATCGTAAGTGTTCTACTACATGCTTATTTAAGG
GCACCTGTAACGCAGTAACT

Downstream 100 bases:

>100_bases
CTGCTACAAGGAACATATCCATTCATAAGCTAAGTTAATAACCCTAATCCACATATCCCATTGTATCAAATGCAACTAGA
ATTACAGCAACCAGGTAGCC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Maltase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSEQIKNIKICIIGGGSHTFIASILRDIALTKSIHGITLTLMDIDEHRLARSYMLARKYFDELKVPINLERTTDTKACIE
GASFVINLAFAIGYDHWGIQVEAAERHGYYRGIDATEWNMVCCYPSLTGFKQYNVALKIAGIMDEINRDAWLIQVSNPVL
ETTTLVHRQYPKLKIIGYCHGAPGGVRLLVEKALKLDMRRIEWQAVGLNHVVFLTRFKYNGEDAYHLIDEWIEKKAEEFW
ASYVPGPWEETLSRAAVDMYRLYGLYPLGDTARSGTWKYHRDLKTKIYWYGPIGGVDSEVGWGIRMLRNQEAEAKLENAA
FNPSIKATEAYPPVKSGEQIIDFIDSVVNNVERRMILNIPNNGVLPRLPSDAIVEAPVYVKGEVIRPEAIENVPNKMYSY
VWYPRIAVTERALEAYLAGSKELLIEALMFDPRTKSTEQAREVIDEILNLPFNEDMKKHYK

Sequences:

>Translated_461_residues
MSEQIKNIKICIIGGGSHTFIASILRDIALTKSIHGITLTLMDIDEHRLARSYMLARKYFDELKVPINLERTTDTKACIE
GASFVINLAFAIGYDHWGIQVEAAERHGYYRGIDATEWNMVCCYPSLTGFKQYNVALKIAGIMDEINRDAWLIQVSNPVL
ETTTLVHRQYPKLKIIGYCHGAPGGVRLLVEKALKLDMRRIEWQAVGLNHVVFLTRFKYNGEDAYHLIDEWIEKKAEEFW
ASYVPGPWEETLSRAAVDMYRLYGLYPLGDTARSGTWKYHRDLKTKIYWYGPIGGVDSEVGWGIRMLRNQEAEAKLENAA
FNPSIKATEAYPPVKSGEQIIDFIDSVVNNVERRMILNIPNNGVLPRLPSDAIVEAPVYVKGEVIRPEAIENVPNKMYSY
VWYPRIAVTERALEAYLAGSKELLIEALMFDPRTKSTEQAREVIDEILNLPFNEDMKKHYK
>Mature_460_residues
SEQIKNIKICIIGGGSHTFIASILRDIALTKSIHGITLTLMDIDEHRLARSYMLARKYFDELKVPINLERTTDTKACIEG
ASFVINLAFAIGYDHWGIQVEAAERHGYYRGIDATEWNMVCCYPSLTGFKQYNVALKIAGIMDEINRDAWLIQVSNPVLE
TTTLVHRQYPKLKIIGYCHGAPGGVRLLVEKALKLDMRRIEWQAVGLNHVVFLTRFKYNGEDAYHLIDEWIEKKAEEFWA
SYVPGPWEETLSRAAVDMYRLYGLYPLGDTARSGTWKYHRDLKTKIYWYGPIGGVDSEVGWGIRMLRNQEAEAKLENAAF
NPSIKATEAYPPVKSGEQIIDFIDSVVNNVERRMILNIPNNGVLPRLPSDAIVEAPVYVKGEVIRPEAIENVPNKMYSYV
WYPRIAVTERALEAYLAGSKELLIEALMFDPRTKSTEQAREVIDEILNLPFNEDMKKHYK

Specific function: Alpha-glycosidase with a very broad specificity. Hydrolyzes maltose and other small maltooligosaccharides but is inactive against the polymeric substrate starch. AglA is not specific with respect to the configuration at the C-4 position of its substrates

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=453, Percent_Identity=23.841059602649, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 52674; Mature: 52543

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIKNIKICIIGGGSHTFIASILRDIALTKSIHGITLTLMDIDEHRLARSYMLARKYF
CCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
DELKVPINLERTTDTKACIEGASFVINLAFAIGYDHWGIQVEAAERHGYYRGIDATEWNM
HHCCCCEEEECCCCHHHHHCCCHHEEEEEHHCCCCCCCEEEEECCCCCEECCCCCCCCCE
VCCYPSLTGFKQYNVALKIAGIMDEINRDAWLIQVSNPVLETTTLVHRQYPKLKIIGYCH
EEECCCCCCCEEECEEEEEEHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEE
GAPGGVRLLVEKALKLDMRRIEWQAVGLNHVVFLTRFKYNGEDAYHLIDEWIEKKAEEFW
CCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
ASYVPGPWEETLSRAAVDMYRLYGLYPLGDTARSGTWKYHRDLKTKIYWYGPIGGVDSEV
HHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCC
GWGIRMLRNQEAEAKLENAAFNPSIKATEAYPPVKSGEQIIDFIDSVVNNVERRMILNIP
CCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECC
NNGVLPRLPSDAIVEAPVYVKGEVIRPEAIENVPNKMYSYVWYPRIAVTERALEAYLAGS
CCCCCCCCCCCCEEECCEEEECCEECCHHHHHCHHHHHHHEECCHHHHHHHHHHHHHCCC
KELLIEALMFDPRTKSTEQAREVIDEILNLPFNEDMKKHYK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
SEQIKNIKICIIGGGSHTFIASILRDIALTKSIHGITLTLMDIDEHRLARSYMLARKYF
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHH
DELKVPINLERTTDTKACIEGASFVINLAFAIGYDHWGIQVEAAERHGYYRGIDATEWNM
HHCCCCEEEECCCCHHHHHCCCHHEEEEEHHCCCCCCCEEEEECCCCCEECCCCCCCCCE
VCCYPSLTGFKQYNVALKIAGIMDEINRDAWLIQVSNPVLETTTLVHRQYPKLKIIGYCH
EEECCCCCCCEEECEEEEEEHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEE
GAPGGVRLLVEKALKLDMRRIEWQAVGLNHVVFLTRFKYNGEDAYHLIDEWIEKKAEEFW
CCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
ASYVPGPWEETLSRAAVDMYRLYGLYPLGDTARSGTWKYHRDLKTKIYWYGPIGGVDSEV
HHCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCC
GWGIRMLRNQEAEAKLENAAFNPSIKATEAYPPVKSGEQIIDFIDSVVNNVERRMILNIP
CCHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECC
NNGVLPRLPSDAIVEAPVYVKGEVIRPEAIENVPNKMYSYVWYPRIAVTERALEAYLAGS
CCCCCCCCCCCCEEECCEEEECCEECCHHHHHCHHHHHHHEECCHHHHHHHHHHHHHCCC
KELLIEALMFDPRTKSTEQAREVIDEILNLPFNEDMKKHYK
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571; 10972187; 12062450; 12588867 [H]